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Citations for Millipore Sigma :
3151 - 3200 of 4915 citations for Recombinant Human CTLA4 Fc His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2024Quote: ... 10% (v/v) heat-inactivated human male AB serum (Sigma-Aldrich) and 6,000 IU/mL recombinant human IL-2 (rhIL-2 ...
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bioRxiv - Cell Biology 2024Quote: ... The coverslips were coated with either 1x Fibronectin human plasma (Sigma) or poly-D-lysine (20 µg/mL) ...
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bioRxiv - Cancer Biology 2024Quote: ... fibrinogen (final conc. of 7.35 mg/mL, from human plasma, Sigma), thrombin (from human plasma ...
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bioRxiv - Immunology 2024Quote: Human umbilical vein endothelial cells (HUVECs, #CRL-1730™, Sigma-Aldrich) were maintained in Endothelial Cell Growth Medium (#211-500 ...
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bioRxiv - Biochemistry 2024Quote: 16HBE14o- (human bronchial airway epithelial cells) were purchased from EMD Millipore Corporation (Temecula ...
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bioRxiv - Biochemistry 2024Quote: ... and human CNS tissues were extracted with RIPA lysis buffer (Millipore) containing the protease/phosphatase inhibitor cocktail (Thermo Fisher) ...
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bioRxiv - Neuroscience 2024Quote: ... Human cell line samples: mouse anti-β-Actin (Sigma-Aldrich, A1978); rabbit anti-PARP (Cell Signalling Technology ...
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bioRxiv - Immunology 2024Quote: Human renal proximal tubule epithelial cells (RPTECs, SA7K clone, MTOX1030; Sigma) were cultured on PureCol-coated T75 flasks (431464U ...
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bioRxiv - Cell Biology 2024Quote: ... were cultured with Human Mammary Epithelial Cell Growth Medium (Sigma-Aldrich). HeLa (ATCC ...
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bioRxiv - Neuroscience 2024Quote: ... blocked in 1% human serum albumin in TBS (HSA; Sigma-Aldrich) containing 0.05% Triton X-100 (Bio-Rad Laboratories ...
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bioRxiv - Neuroscience 2024Quote: ... and FGF (Fibroblast growth factor basic human, Sigma-Aldrich, Cat#SRP6159) at 37°C ...
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bioRxiv - Immunology 2024Quote: ... 4 μl/ml of human fibronectin (1 mg/ml; Sigma-Aldrich), and 10 μl of 1 N NaOH (Sigma-Aldrich ...
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Characterization of pyruvate dehydrogenase complex E1 alpha and beta subunits of Mycoplasma synoviaebioRxiv - Microbiology 2019Quote: ... Recombinant rMSPDHA and rMSPDHB were then expressed through 1.0 mM isopropyl β-D-1-thiogalactopyranoside (IPTG) induction and purified using Ni-NTA His-Bind® Resin Kit (Novagen, San Diego, USA). The purified protein products were quantified by using BCA Protein Assay Kit (Thermo Scientific-Pierce ...
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bioRxiv - Plant Biology 2019Quote: ... and axr3-3 were expressed as 6X His-tag (N-terminal) fusion proteins in Escherichia coli strain Rosetta™ DE3 competent cells (Supplementary Table 4; Novagen, product code: 70954). These proteins were expressed in minimal media with 13C D-glucose and 15N ammonium chloride ...
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bioRxiv - Molecular Biology 2019Quote: ... and zinc binding proteins were eluted according to the supplied protocol using HIS-Select wash and elution buffers purchased from Sigma-Aldrich (Saint Louis, MO).
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bioRxiv - Genomics 2022Quote: ... or T-175 flasks (Hi-C, CUT&RUN) at a density of 1 × 105 cells/mL and treated with 25 nM PMA (Sigma-Aldrich, cat # P1585-1MG). After 24h ...
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bioRxiv - Cancer Biology 2020Quote: ... All hTERT-HME1-derived cells lines were maintained in DMEM/F12 medium (Pan-Biotech, Germany) supplemented with 10% FCS (Sigma-Aldrich, Germany), 2mM L-glutamine (Pan-Biotech ...
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bioRxiv - Immunology 2020Quote: Cell lines were grown at 37°C in either RPMI 1640 medium supplemented with 10% heat-inactivated fetal calf serum (FCS) (Sigma-Aldrich, UK), 100 U/ml Penicillin-Streptomycin ...
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bioRxiv - Cell Biology 2020Quote: ... Emulsions were formed by flow-focusing the collagen/cell solution and oil (fluorocarbon oil FC-40 (Sigma Aldrich, St. Louis, MO, USA)) with 2% FluoroSurfactant (RAN Biotechnologies ...
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bioRxiv - Microbiology 2022Quote: ... At 1 h post infection (hpi) the infection medium was exchanged with DMEM/10% FCS containing 100 µg/ml gentamicin (GEN, Sigma-Aldrich G1397) and increasing concentrations of CIP (0 ...
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bioRxiv - Neuroscience 2019Quote: ... Fixed brain samples were placed securely one at a time in a custom-made MR-compatible holder and immersed in proton-free susceptibility matching fluid (FluorinertTM FC-70; Sigma-Aldrich, UK). Samples were scanned in a random order ...
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bioRxiv - Cell Biology 2020Quote: ... 24h post-plating the cardiomyocytes were fed with fresh medium supplemented with 2% FCS and with 10-5 M Isoproterenol (Sigma I-2760). To induce proliferation ...
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bioRxiv - Biophysics 2020Quote: ... supplemented with 10% heat inactivated fetal calf serum (FCS; PAA, Cölbe, Germany) and 50 U/ml penicillin plus 5 μg/ml streptomycin (Sigma-Aldrich, Munich, Germany). Peripheral blood mononuclear cell (PBMCs ...
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bioRxiv - Immunology 2019Quote: Small intestinal biopsies were collected at the time of endoscopy and transported in R10 (RPMI-1640 [Lonza] + 10% FCS [Sigma-Aldrich] + 1% penicillin/streptomycin [Sigma-Aldrich]), before cryopreservation in freezing medium (10% DMSO [Sigma-Aldrich] ...
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bioRxiv - Microbiology 2022Quote: ... 25 µL 5% FC-40 oil (RAN Biotechnologies, CN 008-FluoroSurfactant-5wtF) was added to the bottom and 50 µL mineral oil (Sigma, CN M5904) was added on the top ...
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bioRxiv - Neuroscience 2022Quote: ... containing 10 % fetal calf serum (FCS) as well as Pen/Strep (50 U/ml penicillin + 50 μg/ml streptomycin; Sigma-Aldrich, USA). After centrifugation at 900 g ...
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bioRxiv - Immunology 2022Quote: ... and transferred in the digestion medium (HBSS with Ca2+ Mg2+ - Fetal Calf serum (FCS) 2% - DNase I 0.1mg/mL (Sigma-Aldrich, ref 11284932001) – Collagenase IV 0.5mg/mL (Sigma-Aldrich ...
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bioRxiv - Microbiology 2024Quote: ... the infected cells were washed twice and further incubated in DMEM/10% FCS containing 100 μg/ml gentamicin (GEN, Sigma-Aldrich G1397) to remove extracellular bacteria ...
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bioRxiv - Immunology 2023Quote: ... Antibody binding to S1 and RBD antigen was detected using a three-step approach consisting of anti-mouse or antirat Fc biotin (Sigma Aldrich B7401) followed by streptavidin-HRPO (Life Technologies cat# SA1007 ...
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bioRxiv - Immunology 2023Quote: ... CD44-FUT7-Fc collected from cell culture medium was concentrated 200-fold using Amicon® Ultra-15 centrifugal filter units (Millipore Sigma), and 10µl of this concentrate was added to block the HUVEC substrate for 1h before performing the rolling assay at 1dyn/cm2 ...
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bioRxiv - Bioengineering 2023Quote: ... with 10% (v/v) Fetal Calf Serum (FCS, First Link, UK) and 1% (v/v) 10 mM L-Glutamine (Sigma-Aldrich, UK). Trypsin with 1 mM ethylenediaminetetraacetic acid (EDTA ...
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bioRxiv - Immunology 2023Quote: ... bone marrow from C57BL/6J mice was treated with ACK and cultured in DC medium (RPMI-1640 [Thermo Fisher Scientific], 10% FCS, 100 U/mL Penicillin, 100 μg/mL Streptomycin, 2 mM L-glutamine [Sigma-Aldrich]) with GM-CSF (20 ng/ml ...
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bioRxiv - Cell Biology 2024Quote: ... were thawed and washed with Dulbecco’s Phosphate-Buffered Saline (DPBS) supplemented with 5% foetal calf serum (FCS) once before incubating with 1mg/ml collagenase P (Sigma Aldrich, UK) and 100μg/ml DNase I (Sigma Aldrich ...
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bioRxiv - Immunology 2024Quote: ... MB49 was cultured in RPMI media (Biochrom GmbH) supplemented with 10% FCS (Biochrom GmbH) and 50 μg/ml Gentamycin (Sigma, Darmstadt, Germany).
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bioRxiv - Cancer Biology 2021Quote: ... Kinase assays were performed as described in (20) using 200ng of purified EPHA2 recombinant protein (OriGen Technologies) or indicated concentration of purified Enolase (Sigma Aldrich), in the absence or presence of about 50ng of purified Src-GFP (or Src3A-GFP as indicated ...
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bioRxiv - Evolutionary Biology 2022Quote: ... blocked in 10% NGS in PBST at 4°C for 3 hr and immunohistochemistry steps were carried out by incubating the samples with primary anti-pH3 antibody (diluted 1:300 in blocking buffer, REF 05-817R, clone 63-1C-8, recombinant rabbit monoclonal antibody, Millipore, USA) for overnight at 4°C as previously described [37–39] ...
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bioRxiv - Cell Biology 2021Quote: ... Base media for the culture of mESCs were supplemented with 1000 units/mL ESGRO Recombinant mouse LIF protein (EMD Millipore ESG1107), and 2i small molecule inhibitors ...
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bioRxiv - Biochemistry 2021Quote: ... The membrane was cut in two (one half for the commercial and one half for the recombinant anti-FLAG™-M2) and each half was probed with either commercial (Sigma) or recombinant anti-FLAG™-M2 at 1 µg/mL in PBS for 45 minutes ...
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bioRxiv - Microbiology 2021Quote: ... both Bactrian camel and llama AEC cultures were treated with recombinant universal type I and III IFNs (100 IU/ml; Sigma Aldrich) or recombinant type III IFN (100 ng/ml ...
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bioRxiv - Microbiology 2020Quote: ... strain BL21(DE3) was used for the production of recombinant proteins expressed from the pET series of plasmids (Novagen Sigma-Aldrich). All L ...
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bioRxiv - Microbiology 2020Quote: ... strain BL21(DE3) was used for the production of recombinant proteins expressed from the pET series of plasmids (Novagen Sigma-Aldrich). All L ...
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bioRxiv - Microbiology 2020Quote: ... mouse polyclonal antibody was produced immunizing a mouse with 50 mg of purified recombinant MtEPSPS containing Freund’s incomplete adjuvant (Sigma-Aldrich, USA) (total volume of 100 µL ...
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bioRxiv - Developmental Biology 2022Quote: ... The dish was then incubated with 40 µg/mL mouse E-cadherin recombinant protein (8875-EC-050, Bio-Techne) or 40 µg/mL fibronectin (F1141, Sigma-Aldrich) at 4 □ overnight ...
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bioRxiv - Cell Biology 2022Quote: ... were used as a substrate for phospho-PKCα in a 25 µl in vitro kinase reaction using 100 ng of active recombinant PKCα enzyme (Millipore-Sigma, MA), 5 µl of a lipid activator (Millipore-Sigma ...
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bioRxiv - Molecular Biology 2020Quote: ... Fractions containing the respective recombinant proteins were pooled and concentrated using Amicon® Ultra-15 spin concentrators with 10 kDa cut-off (Merck Millipore). Concentrated protein pools were run on a Superdex 200 Increase 10/300 GL ...
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bioRxiv - Immunology 2020Quote: ... extracellular Cn were removed by washing 2X with PBS and the macrophages were cultured for a further 24 h in fresh growth media containing either IFNγ to maintain the M1 polarization state or 100 ng/mL recombinant interleukin-4 (IL-4; Sigma-Aldrich, St ...
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bioRxiv - Neuroscience 2021Quote: ... The recombinant LymCBP HAT domain (200 ng) were incubated with the histone H1 purified from calf thymus (Sigma-Aldrich, 1 μg) and 7.4 kBq of [14C]-acetyl-CoA (Moravek ...
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bioRxiv - Microbiology 2021Quote: ... Sublines expressing a doxycycline-inducible BZLF1 transactivator were produced by culturing 106 LCL cells with the recombinant lentivirus in presence of 8 μg/ml polybrene (TR-1003-G, Sigma-Aldrich) for 24 hours followed by replacement of the infection medium with fresh complete medium ...
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bioRxiv - Neuroscience 2020Quote: ... EAE in bone marrow transplanted male animals was induced in anesthetized 8-10 week-old animals after irradiation by subcutaneous injection at the tail base of 10μg of recombinant rat MOG together with incomplete Freud’s adjuvant 1:1 (Sigma, St Louis, MO). EAE was induced in 10-12weeks old female animals using the same protocol using 10μg MOG ...
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bioRxiv - Biochemistry 2019Quote: ... recombinant mTLR9-ECD was titrated into a solution of oligonucleotide labeled at the 5’ end with Alexa 488 Fluor (Sigma-Aldrich). The oligonucleotide concentration ...