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Citations for Millipore Sigma :
3151 - 3200 of 10000+ citations for Recombinant Human SGSH Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2024Quote: ... A mixture of Protein A agarose (Millipore, 16-157) and Protein G agarose (Millipore ...
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bioRxiv - Cancer Biology 2024Quote: ... Proteins were then transferred onto a PVDF membrane (Millipore), and the membrane was blocked with blocking buffer (TBST + 5% non-fat dry milk ...
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bioRxiv - Biochemistry 2023Quote: ... proteins were concentrated in a 3 kDa concentrator (Millipore).
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bioRxiv - Biochemistry 2023Quote: ... The proteins were transferred to Immobilon-FL (Millipore IPFL00010).
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bioRxiv - Biochemistry 2024Quote: ... proteins were expressed in BL21 (DE3)T1R cells (Sigma) at 18 °C overnight after induction with 1 mM IPTG ...
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bioRxiv - Biophysics 2024Quote: ... the proteins were concentrated using 30kDa MWCO Amicon (Millipore) ultra-centrifugal filters to 10 mg/mL and immediately injected in gel filtration column S200 10/300 (Cytiva ...
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bioRxiv - Cell Biology 2024Quote: ... 20 µL of the Protein G agarose bead (Millipore) slurry was washed and added to the antibody-lysate mixture and incubated for 2 h at 4°C ...
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bioRxiv - Cancer Biology 2024Quote: ... followed by protein G beads (Millipore, Cat. #16-266) to the immuno-complex for 2h ...
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bioRxiv - Cell Biology 2024Quote: ... Proteins were transferred to a PVDF membrane (Merck Millipore) and blocked with 5% skimmed milk-TBST (TBS 10x [Sigma-Aldrich] diluted to 1x in distilled water containing 0.1% Tween 20 [Sigma-Aldrich] ...
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bioRxiv - Molecular Biology 2024Quote: Proteins were precipitated with trichloroacetic acid (TCA; Sigma-Aldrich) at a final concentration of 5% and washed twice with ice-cold acetone ...
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bioRxiv - Molecular Biology 2024Quote: ... Proteins were detected by ECL chemiluminescent detection system (Millipore) in Fusion FX (Vilber Lourmat).
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bioRxiv - Microbiology 2024Quote: ... Protein transfer to a PVDF-FL membrane (Millipore, #05317) was achieved at a current of 2.5 mA/cm2 for one hour ...
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bioRxiv - Neuroscience 2024Quote: ... Proteins were transferred onto PVDF membrane (EMD Millipore IPVH00010) for 2.5 hours at 200 mA in ice-cold transfer buffer (25 mM TrisHCl ...
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bioRxiv - Molecular Biology 2023Quote: ... proteins were reduced in 10 mM dithiothreitol (Sigma Aldrich) for 30 min at 37 °C and alkylated in 55 mM iodoacetamide (Sigma Aldrich ...
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bioRxiv - Physiology 2024Quote: ... Total protein concentrations were quantified by Bradford assay (Sigma). An equal amount of protein (20 µg ...
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bioRxiv - Neuroscience 2024Quote: ... Protein quantification was performed using the BCA kit (Sigma), except for insoluble fractions due to due to formic acid’s denaturation effect ...
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bioRxiv - Microbiology 2024Quote: ... Protein concentration was measured with BCA assay (Merck Millipore).
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bioRxiv - Molecular Biology 2024Quote: ... Proteins were subsequently transferred to PVDF membranes (Merk Millipore) and probed with the appropriate indicated antibodies.
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bioRxiv - Genetics 2024Quote: ... Co-purifying proteins were imaged with anti-HA (Sigma) and anti-VSV (Bethyl Laboratories ...
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bioRxiv - Biochemistry 2024Quote: ... protein digested with Proteinase K (RPROTK-RO, Sigma Aldrich) and released RNA isolated via phenol:chlorophorm phase separation ...
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bioRxiv - Synthetic Biology 2023Quote: ... and ReadyBlueTM protein stain were purchased from Sigma-Aldrich. PUREfrex® 1.0 Solutions II (enzymes) ...
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bioRxiv - Developmental Biology 2023Quote: ... Ezview Red Protein G Affinity Gel (Sigma-Aldrich, E3403) was used for negative control samples ...
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bioRxiv - Genomics 2023Quote: ... Proteins were reduced with 50 mM dithiothreitol (Sigma-Aldrich) at 95°C for 10 min and alkylated for 30 min with 40 mM iodoacetamide (Pierce) ...
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bioRxiv - Immunology 2023Quote: ... filtered through 0.45 μm low- protein-binding filters (Millipore) and concentrated via 16 hours centrifugation at 3000 x g at 10 °C ...
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bioRxiv - Cell Biology 2023Quote: ... Protein bands were blotted onto a nylon membrane (Millipore). Blots were blocked using 5% nonfat dry milk (NFDM ...
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bioRxiv - Systems Biology 2023Quote: ... proteins were transferred to a PVDF membrane (Merck Millipore) after which the membrane was blocked using Odyssey Blocking buffer (LI-COR ...
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bioRxiv - Genomics 2022Quote: ... bound to Protein A-Sepharose beads (Sigma-Aldrich, #P6649). Co-purification was investigated by western blot with an anti-GFP antibody (Roche #11814460001 ...
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bioRxiv - Biochemistry 2022Quote: ... The proteins were transferred to PVDF-FL membranes (Millipore) as previously published (Michele et al. ...
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bioRxiv - Biophysics 2022Quote: All Proteins and fluorophores were procured from Sigma-Aldrich and concentration was measured using Shimazdu uv-1900 spectrophotometer ...
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bioRxiv - Immunology 2022Quote: ... The proteins were transferred onto PVDF membranes (Millipore, USA), then blocked with 5 % non-fat dried milk for 1 h at room temperature (RT) ...
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bioRxiv - Cell Biology 2023Quote: ... levels were normalized to total protein levels (Sigma-Aldrich, #E3138 ...
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bioRxiv - Cell Biology 2023Quote: ... Proteins were transferred onto a Immobilon PVDF membrane (Millipore), which was then blocked in 4% milk in tris-buffered saline containing 0.1% Tween-20 (TBST ...
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bioRxiv - Microbiology 2022Quote: ... Samples were precleared with Protein A agarose beads (Millipore), immunoprecipitated with anti-REV-ERBα (NRIDI ...
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bioRxiv - Cell Biology 2023Quote: ... proteins were reduced in 10mM dithiothreitol (Sigma Aldrich, UK) for 30mins at 37ÅãC and alkylated in 55mM iodoacetamide (Sigma Aldrich ...
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bioRxiv - Biochemistry 2023Quote: ... 60µL of Protein A beads (EMD Millipore, 16-156) were washed in IP lysis buffer then blocked for 1hour in 1%BSA in PBS ...
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bioRxiv - Cell Biology 2023Quote: ... Protein G Sepharose 4B fast flow (P3296, Sigma Aldrich) was used for the Co-immunoprecipitation and ChIP assay ...
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bioRxiv - Cell Biology 2023Quote: ... incubated with ReadyBlue Protein Gel Stain (Sigma RSB-1L) overnight for Coomassie staining ...
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bioRxiv - Cell Biology 2023Quote: Proteins were transferred to Immobilon-FL PVDF membranes (Millipore) using a wet blot transfer system ...
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bioRxiv - Biochemistry 2023Quote: ... Proteins were dimethylated by adding 30 mM formaldehyde (Sigma) and 30 mM sodium cyanoborohydride (Sigma ...
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bioRxiv - Cell Biology 2023Quote: ... Proteins were visualized using enhanced chemiluminescent detection kit (Millipore). To detect changes in the protein glycosylation ...
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bioRxiv - Biochemistry 2023Quote: ... After a preclearing step with Protein G Sepharose (Sigma), equal amounts of proteins were then incubated with anti-Flag Beads (Sigma) ...
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bioRxiv - Cell Biology 2023Quote: ... Total protein concentrations were quantified by Bradford assay (Sigma). An equal amount of protein (20 µg ...
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bioRxiv - Molecular Biology 2023Quote: ... Protein concentration was determined by Bradford assay (Sigma-Aldrich) following manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... Proteins were reduced with 10 mM dithioerythritol (Merck-Millipore) for 1 h at 56°C followed by a washing-drying step as described above ...
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bioRxiv - Immunology 2023Quote: Protein samples were boiled in Laemmli buffer (Sigma-Aldrich) at 95°C for 5 min and separated on a 12% Bis-Tris gel (NuPAGE ...
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bioRxiv - Cell Biology 2023Quote: ... proteins were transferred to Nitrocellulose membranes (GE10600012, Sigma-Aldrich). Nitrocellulose membranes were blocked with 5% (w/v ...
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bioRxiv - Neuroscience 2023Quote: ... Protein transfer was confirmed using Ponceau S (Sigma-Aldrich), and blots were cut into two at relevant molecular weights as needed ...
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bioRxiv - Biochemistry 2023Quote: ... Protein constructs were expressed in BL21 (DE3) cells (Novagen), in 2xYT media ...
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bioRxiv - Neuroscience 2023Quote: ... Protein quantification was determined by Bradford reagent (Sigma Aldrich).
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bioRxiv - Molecular Biology 2023Quote: ... Protein was then transferred to a PVDF membrane (Millipore) overnight with NuPAGE transfer buffer ThermoFisher ...