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Citations for Millipore Sigma :
2951 - 3000 of 10000+ citations for Recombinant Human SGSH Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2020Quote: ... Proteins were transferred to a nitrocellulose membrane (Merck Millipore) for 1h at 20V ...
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bioRxiv - Molecular Biology 2021Quote: ... Protein G Sepharose (Millipore Sigma Cat. GE17-0618-01) preblocked in 5% BSA was added and incubated for 1 additional hour rotating at 4°C ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... Protein concentration was determined using Bradford reagent (Sigma-Aldrich). Total protein extracts (30 μg ...
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bioRxiv - Cancer Biology 2019Quote: ... phosphorylated SR proteins (clone 1H4, 1:500, Millipore, #MABE50), total SR proteins (1;1000 ...
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bioRxiv - Developmental Biology 2019Quote: ... Protein concentration was determined using Direct Detect spectrometer (Millipore). 20 µg of protein per sample was denatured at 70°C for 10 mins in 1× LDS loading buffer ...
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bioRxiv - Bioengineering 2020Quote: ... After concentrating the protein solution with Centriprep concentrators (Millipore), the final working concentration of protein was found to be 100 μg/ml using a BCA Protein Assay kit (Thermo Scientific) ...
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bioRxiv - Cell Biology 2019Quote: ... Proteins were eluted off by stripping buffer (Millipore 2504) and re-probed for total Cx43 using the Sigma anti-rabbit antibody ...
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bioRxiv - Plant Biology 2019Quote: ... the protein concentration was estimated by Bradford (Sigma-Aldrich) and SDS-PAGE analysis (Supplementary Figure S1).
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bioRxiv - Biochemistry 2019Quote: ... Protein was then estimated using Bradford’s reagent (Sigma, USA).
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bioRxiv - Molecular Biology 2019Quote: EZ-Magna RNA-binding protein immunoprecipitation kit (Millipore, USA) was performed according to the manufacture’s protocol ...
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bioRxiv - Molecular Biology 2019Quote: ... Protein emulsion was made in complete Freund’s adjuvant (Sigma) for subcutaneous immunization in two rabbits followed by three successive boosters in incomplete adjuvant at four week intervals.
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bioRxiv - Biophysics 2019Quote: ... Protein estimation was done using Bradford reagent (Sigma Inc.) and stored in −80°C after flash freezing in liquid nitrogen.
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bioRxiv - Biochemistry 2019Quote: ... The supernatants were passed through a Protein A (Sigma) affinity column ...
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bioRxiv - Animal Behavior and Cognition 2019Quote: ... Proteins were transferred to Immobilon-FL PVDF membranes (Millipore) followed by incubation in Odyssey blocking buffer (LI-COR ...
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bioRxiv - Plant Biology 2019Quote: ... After incubation with Protein A agarose beads (Millipore, USA), enriched DNA fragments used as the templates for PCR were eluted out and purified with phenol/chloroform (1:1 ...
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bioRxiv - Cell Biology 2019Quote: ... Protein G Sepharose 4 Fast Flow (P3296 Sigma-Aldrich) were incubated with 2 µg of GFP antibody raised in Goat for 2h at 4°C and were then washed with lysis buffer ...
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bioRxiv - Plant Biology 2019Quote: ... and Protein G magnetic beads (EMD Millipore, Burlington MA) pre-treated with 0.1% (w/v ...
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bioRxiv - Immunology 2019Quote: ... and 20 μl of Magna protein A beads (Millipore). Bound Saa3 promoter sequences were quantified using SYBR Green-based real-time PCR (target sequence and primers listed in Table S4) ...
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bioRxiv - Bioengineering 2019Quote: ... microtubule associated protein 2 (MAP2) (1:500, Sigma-Aldrich). The cells were subsequently washed 6 x 5 min each with PBS/0,1% Triton-X 100 ...
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bioRxiv - Biochemistry 2019Quote: ... Protein was eluted with 10 mM reduced GSH (Sigma) dissolved in 10 mM Tris-HCl ...
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bioRxiv - Plant Biology 2021Quote: ... The eluted protein was concentrated using amicon filters (Millipore). The concentrated protein was then passed through a Q-sepharose (Ion-exchange column ...
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bioRxiv - Genetics 2020Quote: Cellular protein was extracted using RIPA buffer (Sigma, #R0278) with protease inhibitors (Roche ...
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bioRxiv - Cancer Biology 2020Quote: ... Protein concentration was quantified by Bradford (Sigma B6916-500mL) according to manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2020Quote: ... Biotinylated hyaluronan-binding protein (HABP) was supplied by Millipore-Sigma ...
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bioRxiv - Neuroscience 2020Quote: ... Protein concentration was determined using Bradford Reagent (Sigma-Aldrich). At experimental endpoints ...
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bioRxiv - Cell Biology 2021Quote: ... Proteins were transferred to an Immobilon PVDF membrane (Millipore) overnight at 50 milliamps ...
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bioRxiv - Cancer Biology 2021Quote: ... Proteins were then transferred onto PVDF membranes (Immobilon, Millipore) and incubated overnight with primary antibodies at 4°C ...
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bioRxiv - Bioengineering 2020Quote: ... Total protein concentration was assessed using Bradford assay (Sigma) and spectrophotometrically (considering ε515 = 83,000 M-1 cm-1 for Ca2+-free form of cpVenus) ...
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bioRxiv - Biochemistry 2020Quote: ... Proteins were resuspended in 5μL of 8M urea (Sigma) in 100mM ammonium bicarbonate for 10min at 30°C ...
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bioRxiv - Biochemistry 2020Quote: ... protein translation was inhibited with cycloheximide (CHX, Sigma-Aldrich) dissolved in phosphate buffer saline (PBS ...
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bioRxiv - Biochemistry 2020Quote: ... Protein melting was monitored using 1000X SYPRO Orange (Sigma) binding dye ...
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bioRxiv - Biochemistry 2020Quote: ... pH 7.0 and SYPRO Orange protein stain dye (Sigma). Melting curves were recorded with a temperature range of 20 to 95 °C ...
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bioRxiv - Neuroscience 2019Quote: The protein synthesis inhibitor cycloheximide (CHX, Sigma-Aldrich C7698) (Pedreira ...
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bioRxiv - Cell Biology 2021Quote: ... Proteins were electroblotted onto PVDF membranes (IPVH00010, Merck Millipore) via a semi- dry cassette (1703940 ...
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bioRxiv - Cell Biology 2021Quote: ... Proteins were reduced with dithiothreitol (DTT, 5 mM; Sigma) at 55°C for 30 minutes ...
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bioRxiv - Cancer Biology 2020Quote: ... Protein and RNA were degraded by Proteinase K (Sigma) and RNase A (5prime) ...
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bioRxiv - Cancer Biology 2020Quote: ... 40 μl of protein A/G Sepharose beads (Millipore) were added and the lysate was pre-cleared for 1 hr at 4°C ...
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bioRxiv - Neuroscience 2020Quote: ... proteins were transferred to Immobilon-FL PVDF membranes (Millipore) using the NuPage transfer buffer transfer system (Invitrogen ...
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bioRxiv - Neuroscience 2020Quote: ... Npas4 protein was affinity purified on M2 agarose (Sigma) as described previously54 ...
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bioRxiv - Biochemistry 2020Quote: ... For the protein used for electron microscopy thrombin (Sigma) 1:1 w/w was added during dialysis to remove the affinity label) ...
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bioRxiv - Cell Biology 2021Quote: ... proteins were transferred onto a PVDF membrane (Millipore #ISEQ20200) and incubated 1 hour at room temperature with 2% milk powder in PBS ...
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bioRxiv - Plant Biology 2021Quote: All tested proteins and citrate synthase (CS, Sigma, C3260) was dialyzed in 20 mM HEPES-KOH ...
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bioRxiv - Microbiology 2020Quote: ... filtered through a 0.45μm low protein-binding filter (Millipore), and used to transduce Vero cells ...
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bioRxiv - Plant Biology 2021Quote: ... coli BL21 (DE3) for protein production (Novagen, Madison, USA).
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bioRxiv - Plant Biology 2021Quote: ... Retained proteins were alkylated with 50 mM acrylamide (Sigma) in UT buffer for 30 minutes at 20◦C while gently shaking ...
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bioRxiv - Plant Biology 2021Quote: ... proteins were wet-transferred to a nitrocellulose membrane (Millipore). Membranes were blocked in 5% skimmed milk and probed with anti-HA (Roche anti-HA-HRP 3F10) ...
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bioRxiv - Plant Biology 2021Quote: ... The total proteins were quantified by Bradford reagent (Sigma) and purity was analysed by SDS-PAGE ...
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bioRxiv - Microbiology 2020Quote: ... Proteins were transferred onto an immobilon-FL membrane (Millipore) at 75v for 1hr ...
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bioRxiv - Neuroscience 2020Quote: ... rabbit anti-protein kinase C-α (PKCα) (Sigma, P4334); rabbit anti-VSX2 (Chx10 ...
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bioRxiv - Immunology 2021Quote: ... Proteins were then transferred to PVDF membrane (Millipore, 0.45μm). The membranes were first incubated with anti-phospho-Src (Tyr416 ...