Labshake search
Citations for Millipore Sigma :
3101 - 3150 of 7599 citations for Zika Virus Envelope Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Biochemistry 2019Quote: ... The purified protein was concentrated with an Amicon Ultra-10 (Millipore, Billerica, MA) for future NMR study ...
-
Removal of Spo11 from meiotic DNA breaks in vitro but not in vivo by Tyrosyl DNA Phosphodiesterase 2bioRxiv - Molecular Biology 2019Quote: ... and Spo11 was immunoprecipitated from total soluble protein using anti-FLAG antibody (Sigma) and protein-G-agarose (Roche) ...
-
bioRxiv - Biophysics 2019Quote: ... The CbAgo protein was expressed in E.coli Bl21(DE3) Rosetta™ 2 (Novagen). Cultures were grown at 37°C in LB medium containing 50μa.g ml-1 kanamycin and 34μg ml-1 chloramphenicol till an ODóOOnm of 0.7 was reached ...
-
bioRxiv - Molecular Biology 2019Quote: ... plasmids for different Cas9 proteins were expressed in Escherichia coli Rosetta2 (DE3) (Novagen). The protein expressing Rosetta2 (DE3 ...
-
bioRxiv - Genetics 2019Quote: Recombinant proteins were expressed in Escherichia coli BL21(DE3)pLys using pET28a+ (Novagen) and purified using Ni-Sepharose affinity medium (GE Healthcare ...
-
bioRxiv - Neuroscience 2019Quote: ... Proteins were then transferred to an Immobilon-FL PVDF membrane (Millipore, Billerica, MA) on ice either for 2 h at 100 V ...
-
bioRxiv - Molecular Biology 2019Quote: ... Myc-tagged proteins were incubated with an anti-Myc primary antibody 9E10 (Millipore) at a 1:2000 dilution while tubulin was incubated with anti-Tubulin antibody B-5-1-2 (Sigma-Aldrich ...
-
bioRxiv - Neuroscience 2019Quote: ... the protein was transferred to a polyvinylidene difluoride (PVDF) membrane (Immobilon-P; Millipore). The membrane was blocked with 5% skimmed milk and incubated with primary antibodies overnight at 4°C ...
-
bioRxiv - Cell Biology 2019Quote: ... Immunoprecipitated protein/DNA complexes were prepared using the Magna ChIP A kit (Millipore) following manufacture’s instructions ...
-
bioRxiv - Neuroscience 2019Quote: ... Proteins were extracted by homogenizing the tissue in ice-cold RIPA buffer (Sigma) and lysates were centrifuged at 16,000 × g at 4°C ...
-
bioRxiv - Pathology 2019Quote: ... Co-IP assays were performed by incubating total proteins with anti-HA (Sigma) agarose followed by column wash and elution according to manufacturer’s instructions (Pierce ...
-
bioRxiv - Pharmacology and Toxicology 2019Quote: ... The equal loading of the blotted protein was verified by Ponceau S (Sigma) staining ...
-
bioRxiv - Biochemistry 2019Quote: ... and 1% NP-40 containing protein phosphatase inhibitor cocktail 1 and 2 (Sigma) and protease inhibitors (Sigma)] ...
-
bioRxiv - Cell Biology 2019Quote: ... after which the proteins were transferred to a PVDF membrane (Immobilon-P, Millipore). Primary antibodies were incubated overnight at 4 °C in 1% milk ...
-
bioRxiv - Biophysics 2019Quote: ... Fractions containing the purified proteins were concentrated using Amicon Ultra concentrators (Millipore Sigma).
-
bioRxiv - Synthetic Biology 2020Quote: ... Total protein concentration in CFE was determined by Bradford reagent (Sigma-Aldrich, USA), and ∼55 mg of total protein in CFE was incubated for 60 min at 4 °C with 2 mL of Ni-NTA agarose (QIAGEN ...
-
bioRxiv - Microbiology 2019Quote: ... The protein concentration in the extract was determined with a Bradford assay (Sigma) and used for normalisation of the SOD activity.
-
bioRxiv - Cell Biology 2020Quote: ... Protein A or G agarose (Pierce, 20333 or Millipore-Sigma 16-201, respectively) was equilibrated in lysis buffer and added to the lysates at 4°C with gentle rotation ...
-
bioRxiv - Cell Biology 2020Quote: ... Protein A or G agarose (Pierce, 20333 or Millipore-Sigma 16-201, respectively) was equilibrated in lysis buffer and added to the lysates at 4°C with gentle rotation ...
-
bioRxiv - Developmental Biology 2019Quote: ... Proteins were detected with the primary antibodies against Flag (1:1000, Sigma F7425), GFP (1:1000 ...
-
bioRxiv - Systems Biology 2019Quote: ... Proteins were precipitated with 1 μg of anti-Flag antibody (F-3165, Sigma) for 2 h at 4°C followed by the addition of 15 μl protein G-Sepharose beads for 1 h ...
-
bioRxiv - Cell Biology 2020Quote: ... The total nuclear protein was lysed with CHAPS buffer containing protease inhibitor (Sigma) and phosphatase inhibitor (Roche Applied Science ...
-
bioRxiv - Plant Biology 2021Quote: ... Sonicated samples were first precleared with Protein A Agarose beads (Millipore #16-157) and then mixed with antibodies ...
-
bioRxiv - Molecular Biology 2020Quote: ... TAP-tagged proteins were detected with peroxidase–anti-peroxidase– soluble complex (P1291, Sigma). α-tubulin was detected with antibody T5168 (Sigma).
-
bioRxiv - Plant Biology 2020Quote: ... antiserum against FLAG fusion proteins was obtained from Sigma-Aldrich (F1804, mouse monoclonal). An anti-rabbit horseradish peroxidase-conjugated antiserum (#7074 ...
-
bioRxiv - Microbiology 2020Quote: ... The protein-DNA complex was treated with 1 U/μl DNase I (Novagen) to digest non-specific DNA ...
-
bioRxiv - Immunology 2021Quote: ... Protein bands were revealed by enhanced chemiluminescence reagents (ECL™ Prime, Merck Millipore) in a ChemiDoc XRS+ imaging system (BioRad ...
-
bioRxiv - Synthetic Biology 2021Quote: ... Protein was precipitated by adding 1 volume of TCA (trichloroacetic acid, Sigma Aldrich) to 4 volumes of supernatant ...
-
bioRxiv - Microbiology 2020Quote: ... protein containing fractions were pooled and concentrated with an Amicon Ultracel-10K (Millipore) according to the experimental requirements.
-
bioRxiv - Microbiology 2020Quote: ... Proteins in the supernatants were precipitated with 4x volume cold acetone (V800023, Sigma) overnight at −20°C ...
-
bioRxiv - Molecular Biology 2021Quote: ... or proinsulin Ab (CCI-17) bound protein G beads (GE17-0618-01, Sigma) for overnight at 4°C ...
-
bioRxiv - Neuroscience 2020Quote: ... and proteins were transferred onto polyvinylidene difluoride (PVDF) membranes (Merck Millipore, Chengdu, China) for 2 h at 150 mA in 10% transfer buffer [3% Tris–HCl (Aladdin Industrial Corporation ...
-
bioRxiv - Neuroscience 2020Quote: ... A mixture of primary antibodies against enhanced green fluorescent protein (EGFP: chicken, Millipore), human influenza hemagglutinin (HA ...
-
bioRxiv - Neuroscience 2020Quote: ... mouse anti-microtubule associated protein (MAP2; 1:1,000; Sigma‐Aldrich; St Louis, USA); DAPI (1:2,000 ...
-
bioRxiv - Biochemistry 2020Quote: ... ATP11A protein was labeled with mouse anti-Flag antibody (1:2000, Sigma, Germany) and the endoplasmic reticulum (ER ...
-
bioRxiv - Biochemistry 2020Quote: ... Tie2 protein was immunoprecipitated from the lysates by using anti‐Tie2 antibody (Millipore) and protein A/G‐sepharose ...
-
bioRxiv - Neuroscience 2020Quote: ... depending on respective protein molecular weight) and transferred on a PVDF membrane (Millipore). Blots were blocked with 5% milk powder or 5% BSA in TBS + 0.1% Tween-20 (TBST ...
-
bioRxiv - Immunology 2020Quote: ... MaxiSorp plates were coated with 1 μg/mL HIV-GP120 protein (Sigma-Aldrich). Samples were diluted in an eight-point two-fold dilution series ...
-
bioRxiv - Microbiology 2021Quote: ... The lysate was mixed with 40 μL protein G agarose (Millipore, United States) to avoid nonspecific binding for 2 h at 4 °C ...
-
bioRxiv - Molecular Biology 2019Quote: ... Protein concentration was measured using a standard Bradford assay reagent (B6916, Sigma Aldrich).40µg total protein were loaded per samples on a pre-cast gradient 4-12% gel (NW04120 ...
-
bioRxiv - Molecular Biology 2019Quote: ... The proteins were concentrated using Amicon Ultracel-30 Centrifugal Filter Units (Merck Millipore) for wild-type RF2 and RF2-GGP and Ultracel-3 for ResQ62-His6 hereafter referred to as ResQ.
-
bioRxiv - Microbiology 2019Quote: ... Bound His-tagged proteins were detected using HRP-conjugated anti-His antibodies (Sigma) at a 1:500 dilution ...
-
bioRxiv - Immunology 2019Quote: ... 6 μl (=10 μg) total protein was then reacted with streptavidin (Sigma, S4762) in PBS (total reaction volume 24 μl ...
-
bioRxiv - Molecular Biology 2019Quote: Proteins were separated on 5.5% polyacrylamide gel and transferred on PVDF membrane (Millipore) using semi-dry blotting for 50 min ...
-
bioRxiv - Genetics 2020Quote: Proteins were reduced with adding 1.5 μl of 200 mM dithiothreitol (DTT, Sigma) in 500 mM AmBic and incubated at 37 °C for 1 h with shaking at 400 rpm ...
-
bioRxiv - Cell Biology 2020Quote: ... Myc-tagged proteins were immunoprecipitated with 4AG Myc monoclonal agarose beads (EMD Millipore), washed three times in lysis buffer and boiled in 2X Laemmli reducing buffer (Bio-Rad) ...
-
bioRxiv - Cell Biology 2021Quote: ... Proteins were transferred to a Immobilion-P PVDF membrane (Millipore Sigma, MA, USA) at 47V overnight at 4°C using Mini Trans-Blot® Cell (Bio-Rad Laboratories ...
-
bioRxiv - Molecular Biology 2020Quote: ... Fractions containing protein were concentrated in 50 kDa cutoff Amicon centrifugal filters (Millipore). Aliquots were frozen in dry ice and stored at −80 °C.
-
bioRxiv - Molecular Biology 2020Quote: ... fractions containing protein were concentrated in 10 kDa cutoff Amicon centrifugal filters (Millipore). Aliquots were frozen in dry ice and stored at −80 °C.
-
bioRxiv - Neuroscience 2021Quote: ... Proteins were detected by the ECL method (Immobilon Western Chemiluminescent HRP Substrate, Millipore), and loading control of the western blot membrane was performed using the Revert total protein stain (LI-COR Bioscience).