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Citations for Millipore Sigma :
2951 - 3000 of 7599 citations for Zika Virus Envelope Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2019Quote: ... Samples were precleared by a 30 minute-incubation with Protein G agarose (Sigma), and then incubated for 2 hours at 4°C with Protein G agarose and 5 µg of indicated antibodies ...
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bioRxiv - Biochemistry 2019Quote: ... and the protein concentration was assessed using a BCA kit (Sigma-Aldrich, USA). Samples were diluted to equal concentrations and separated using the Novex Native PAGE Bis–Tris Gel system (4–16% ...
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bioRxiv - Immunology 2019Quote: ... Proteins were detected using Luminata™ Forte Western HRP Substrate (Millipore, Watford, UK) visualized on Hyperfilm™ (GE Healthcare ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... proteins were electro-transferred to a polyvinylidene difluoride membrane (Merck Millipore, Darmstadt, Germany), which was incubated in the blocking buffer (5% non-fat milk ...
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bioRxiv - Immunology 2019Quote: ... SDS-PAGE separated proteins were transferred onto Immobilon-FL PVDF membranes (EMD Millipore) at 100V for 1h at 4°C ...
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bioRxiv - Cell Biology 2020Quote: ... Immunoreactive proteins were visualized by Immobilion western chemiluminescence substrate (Merck-Millipore cat#WBKLS0500) [7].
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bioRxiv - Biophysics 2020Quote: ... and 10 μL of 5 mg∙mL−1 of protein A (Sigma-Aldrich), were added to 1 mL of 10 mg∙mL−1 of N-(3-dimethylaminopropyl)-N’-ethylcabodiimidie hydrochloride (EDC ...
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bioRxiv - Immunology 2020Quote: Protein lysates were subjected to SDS-PAGE and transferred to PVDF membranes (Millipore). Membranes were blocked and probed with one of the following antibodies ...
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bioRxiv - Bioengineering 2019Quote: ... Antibodies were purified from medium using protein G gravitrap (Sigma, 28-9852-55) according to manufacturer protocol ...
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bioRxiv - Microbiology 2019Quote: ... proteins precipitated on the beads were eluted using Laemmli’s buffer containing dithiothreitol (Sigma) boiled 5 min at 95 °C ...
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bioRxiv - Developmental Biology 2020Quote: ... protein synthesis was inhibited by treating cells with 350 µM cycloheximide (Sigma Aldrich).
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bioRxiv - Cell Biology 2019Quote: ... and DNA loaded proteins were released by incubation with S7 nuclease (Sigma Aldrich) in complete CSK at room temperature (RT ...
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bioRxiv - Immunology 2019Quote: ... Streptomyces hyaluronlyticus hyaluronidase (HA’se) and biotinylated HA-binding protein (HABP) were from Millipore, and streptavidin was from Thermo Fisher Scientific or eBioscience ...
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bioRxiv - Microbiology 2019Quote: ... Proteins were detected by western blot using anti-FLAG (1:1,000 dilution; Sigma), anti-hemagglutinin (HA ...
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bioRxiv - Molecular Biology 2019Quote: ... FLAG-CTCF-6xHis protein was induced in Rosseta-Gami B-cells (EMD Millipore) with 0.2 M of IPTG at room temperature and was purified with Ni-NTA resin (Qiagen ...
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bioRxiv - Biochemistry 2019Quote: ... The protein was then concentrated with a 10K Amicon Ultra-15 concentrator (Millipore) and diluted into Storage Buffer (50 mM Tris pH 7.5 ...
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bioRxiv - Immunology 2020Quote: CD200 and 41BB FAST proteins were digested overnight with TEV protease (Sigma # T4455) at 4 °C in PBS ...
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bioRxiv - Neuroscience 2019Quote: ... anti-microtubule associated protein 2 (MAP2, 1:500, mouse monoclonal, Sigma, St.Louis, MO), GluN2B (1:1000 ...
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bioRxiv - Cancer Biology 2020Quote: ... The protein fractions were isolated and concentrated using 3kDa centrifugal filters (Merck Millipore). LC MS/MS was performed on the concentrated culture CMed and spectral analysis was performed ...
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bioRxiv - Biochemistry 2019Quote: ... and protein concentration was measured using a Bradford assay (Sigma-Aldrich, Taufkirchen, Germany). Proteins were separated with a 10% SDS-PAGE ...
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bioRxiv - Plant Biology 2020Quote: ... cells were harvested and lysed using BugBuster Protein Extraction Reagent (Novagen, Nottingham, UK), soluble fractions were used over gravity flow Ni2+-NTA sepharose columns (1 ml ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Total proteins were spotted (10 μg) onto nitrocellulose membranes (Merck Millipore, Darmstadt, Germany) and left to dry ...
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bioRxiv - Genomics 2019Quote: ... and then protein bands were detected using Horseradish peroxidase-conjugated secondary antibodies (Sigma) and Clarity Western ECL Substrate according to manufacturer’s instructions (BioRad) ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Total proteins were spotted (10 μg) onto nitrocellulose membranes (Merck Millipore, Darmstadt, Germany) and left to dry ...
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bioRxiv - Neuroscience 2019Quote: ... and the proteins were transferred to a pre-activated PVDF membrane (Millipore, IPFL00005) at −70 mV for 3 to 4 hours ...
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bioRxiv - Cell Biology 2019Quote: ... Proteins were transferred to a polyvinylidene difluoride (PVDF) membrane (EMD Millipore, Toronto, ON), and blocked in 5% skim milk or BSA ...
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bioRxiv - Cell Biology 2019Quote: ... Total protein bands were reversibly stained with Fast Green FCF (Sigma-Aldrich, F7252) and visualized on ImageQuant LAS 4000 (GE Healthcare Life Sciences ...
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bioRxiv - Biochemistry 2020Quote: ... Protein was eluted using purification buffer supplemented with 5 mM D-desthiobiotin (Sigma). Following affinity purification ...
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bioRxiv - Developmental Biology 2021Quote: ... The proteins were transferred to a polyvinylidene fluoride (PVDF) membrane (Sigma-Aldrich, ISEQ00010) and blocked in 5% fat-free milk for one hour at room temperature ...
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bioRxiv - Plant Biology 2020Quote: ... proteins were eluted with 150 μg.ml-1 3× FLAG peptide (Sigma-Aldrich, F4799) in extraction buffer ...
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Dual signaling via interferon and DNA damage response elicits entrapment by giant PML nuclear bodiesbioRxiv - Microbiology 2021Quote: ... FLAG and Myc tagged-proteins were detected with mAb-FLAG M2 (Sigma-Aldrich) and mAb-Myc 1-9E10.2 (ATCC) ...
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bioRxiv - Biochemistry 2021Quote: ... bound protein was eluted with binding buffer containing 2.5 mM d-Desthiobiotin (Millipore) and dialyzed into dialysis buffer (50 mM Tris-HCl ...
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bioRxiv - Biochemistry 2020Quote: ... The Gag-His6 protein was loaded onto a HIS-select affinity column (Sigma), washed (20 mM Tris-HCl ...
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bioRxiv - Biochemistry 2020Quote: ... The protein was treated with > 10-fold excess of TCEP in DMSO (Sigma) and incubated for an hour in the anaerobic chamber at room temperature ...
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bioRxiv - Cell Biology 2021Quote: ... The concentrated protein samples were filtrated through Millex 0.22-μm syringe filters (Millipore) and loaded onto a Superdex 200 HR 10/30 gel-filtration column (GE Healthcare ...
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bioRxiv - Biochemistry 2020Quote: ... The protein was concentrated with a 100 MWCO Amicon centrifugal filter unit (Millipore) and purified by size exclusion chromatography using a Superose 6 Increase 10/300 GL column (GE Healthcare ...
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bioRxiv - Bioengineering 2021Quote: ... Eluted proteins were buffer exchanged and concentrated with PBS using Amicon tubes (Millipore). The proteins were further purified by size exclusion chromatography (SEC ...
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bioRxiv - Microbiology 2021Quote: ... All proteins were concentrated by ultrafiltration with Amicon Ultra (10 kDa, Millipore, Germany) and protein purity was confirmed by SDS– PAGE ...
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bioRxiv - Biochemistry 2021Quote: ... Proteins were concentrated to > 150 μM using centrifugal filters (Amicon Ultra; Merck Millipore), aliquoted and snap-frozen and stored at −80 °C ...
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bioRxiv - Neuroscience 2020Quote: ... Protein transfer was confirmed with Ponceau S stain (0.1% w/v Sigma-Aldrich). Membranes were blocked for 5 minutes using undiluted EveryBlot Blocking Buffer (Bio-Rad ...
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bioRxiv - Neuroscience 2021Quote: ... proteins were transferred to a PVDF membrane (Immobilon-FL; Merck Millipore, Billerica, MA). Immunoblotting was performed with primary antibodies against Camk2a phosphorylated at pT286 (1:1000 ...
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bioRxiv - Neuroscience 2021Quote: ... Protein concentrations were determined using the BCA method (QuantiPro assay kit; Sigma-Aldrich).
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bioRxiv - Neuroscience 2021Quote: ... and total protein was detected with Amido Black (Sigma-Aldrich, St. Louis, MO) as previously described 51 ...
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bioRxiv - Cancer Biology 2021Quote: ... Protein lysates were prepared in RIPA buffer (containing protease (1:100; Sigma, P8340) and phosphatase inhibitors (1:500 ...
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bioRxiv - Cell Biology 2021Quote: ... Proteins were transferred onto 0.2 μm polyvinylidene fluoride (PVDF) membranes (Millipore, Hertfordshire, UK) for 1 hour at 100V ...
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bioRxiv - Biophysics 2021Quote: His-tagged human PCNA protein was purchased from Sigma Aldrich (catalogue number SRP5117), at a concentration of 6.6 μM ...
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bioRxiv - Cell Biology 2021Quote: ... Protein was transferred onto PVDF paper (Millipore Sigma, Cat # IVPH00010, Billerica, MA, USA) for 90 mins at 0.5 amps with a Power Pac HC power supply (Bio Rad ...
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bioRxiv - Cancer Biology 2020Quote: ... Dried protein hydrolysates were re-dissolved in 16µL HPLC grade pyridine (Sigma 270407) prior to derivatization with 20µL N–methyl–N–(tert–butyldimethylsilyl)trifluoroacetamide + 1% tert–Butyldimethylchlorosilane (Sigma 375934 ...
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bioRxiv - Cancer Biology 2020Quote: ... The protein was then transferred onto a polyvinylidene difluoride membrane (Millipore, Toronto, Canada) and probed using antibodies for PI4KIIIβ (BD Biosciences 611817 ...
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bioRxiv - Biochemistry 2021Quote: ... Viral supernatants were filtered (0.45 μm low protein binding filter unit (EMD Millipore)) and concentrated 10-20-fold (Amicon Ultra-15 concentrator with a 100,000-dalton molecular mass cutoff) ...