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Citations for Millipore Sigma :
3101 - 3150 of 10000+ citations for Human Urocortin 3 UCN3 CLIA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... The human lymphoblastoid line BOLETH was obtained from Sigma (#88052031-1VL). B-cell lines were cultured in GlutaMAXTM supplemented RPMI 1640 medium further modified with 10% FBS ...
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bioRxiv - Bioengineering 2023Quote: ... stained using mouse anti-human fibronectin monoclonal antibody (Sigma-Aldrich, US) and detected with goat anti-mouse Alexa Fluor 488 secondary antibody (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2023Quote: ... and fibronectin from human plasma (Sigma-Aldrich F1056; 1 μg/ml). For some experiments ...
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bioRxiv - Cancer Biology 2023Quote: ... and human samples were cryopreserved in 10% DMSO (Sigma-Aldrich #F4135) in FBS in liquid nitrogen at -196°C until use ...
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bioRxiv - Cancer Biology 2023Quote: ... PC3 human cell line was obtained from Sigma Aldrich (90112714-1VL) and cultured in DMEM medium with 10 % FBS.
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bioRxiv - Cancer Biology 2023Quote: PNT2 human cell line was purchased from Sigma Aldrich (95012613-1VL) and cultured in RPMI 1640 containing 2 mM Glutamine and 10 % Foetal Bovine Serum (FBS) ...
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bioRxiv - Cell Biology 2023Quote: ... containing 1% Human Platelet Poor Plasma-derived Serum (SH, Sigma, P2918), 20 ng/mL FGF2 and 10 uM All-trans Retinoic Acid (RA ...
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bioRxiv - Systems Biology 2023Quote: Human insulin (10 mg/ml) was from Sigma (#91077C, lot: 21M018) and stored at 4°C.
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bioRxiv - Biochemistry 2023Quote: ... USA) Albumin from human serum (HSA) was purchased from Sigma-Aldrich Canada ...
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bioRxiv - Cell Biology 2023Quote: Human recombinant Cdk9/cyclin K and CDK9/cyclin T (Sigma Aldrich) were incubated with the substrate at 30°C for 30 minutes in reaction buffer (25 mM Tris-acetate (pH 7.9) ...
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bioRxiv - Bioengineering 2024Quote: ... 500 μg/mL recombinant human albumin (Sigma-Aldrich, cat. no. A9731), and 213 μg/mL L-ascorbic acid 2-phosphate (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2023Quote: ... purified calpain-1 from human or porcine erythrocytes (8 µg, Millipore) or human or rat recombinant calpain-2 produced by bacterial expression as described by (Elce et al. ...
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bioRxiv - Bioengineering 2024Quote: ... anti-human nuclei (HuNu; 1:100; MAB4383, Merck Millipore, MA, USA), anti-Ku80 (1:100 ...
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bioRxiv - Immunology 2024Quote: ... supplemented with 10% (v/v) human AB-serum (Sigma Aldrich,, Germany) seeded into well culture plates and stimulated with antigens for 18 h in presence of 1 mg/ml of anti-CD40 blocking antibody (both Miltenyi Biotec ...
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bioRxiv - Immunology 2023Quote: ... and mouse anti-human IgG4 biotin (Sigma-Aldrich, St Louis, MO), respectively ...
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bioRxiv - Cell Biology 2024Quote: ... were cultured with Human Mammary Epithelial Cell Growth Medium (Sigma-Aldrich). HeLa (ATCC ...
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bioRxiv - Immunology 2024Quote: Human renal proximal tubule epithelial cells (RPTECs, SA7K clone, MTOX1030; Sigma) were cultured on PureCol-coated T75 flasks (431464U ...
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bioRxiv - Neuroscience 2024Quote: ... blocked in 1% human serum albumin in TBS (HSA; Sigma-Aldrich) containing 0.05% Triton X-100 (Bio-Rad Laboratories ...
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bioRxiv - Cancer Biology 2024Quote: ... 10% (v/v) heat-inactivated human male AB serum (Sigma-Aldrich) and 6,000 IU/mL recombinant human IL-2 (rhIL-2 ...
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bioRxiv - Immunology 2024Quote: ... or isotype-control human IgG Ab (10 μg/ml; Sigma-Aldrich) was incubated on de-paraffinized tissue sections overnight at 4°C ...
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bioRxiv - Cancer Biology 2024Quote: ... fibrinogen (final conc. of 7.35 mg/mL, from human plasma, Sigma), thrombin (from human plasma ...
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bioRxiv - Cell Biology 2024Quote: ... The coverslips were coated with either 1x Fibronectin human plasma (Sigma) or poly-D-lysine (20 µg/mL) ...
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bioRxiv - Cancer Biology 2024Quote: ... Plates were coated with FN (Sigma-Aldrich, Darmstadt, Germany, human plasma) and 5x105 cells were seeded and allowed to attach 5-10 min ...
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bioRxiv - Cell Biology 2024Quote: ... recombinant full-length human active AAK (#14-511 Merck Millipore, Germany) were incubated either in the presence or the absence of 14nM of N-terminal GST-fused active HsMPS1/TTK (SignalChem ...
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bioRxiv - Neuroscience 2024Quote: ... Human cell line samples: mouse anti-β-Actin (Sigma-Aldrich, A1978); rabbit anti-PARP (Cell Signalling Technology ...
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bioRxiv - Immunology 2024Quote: Human umbilical vein endothelial cells (HUVECs, #CRL-1730™, Sigma-Aldrich) were maintained in Endothelial Cell Growth Medium (#211-500 ...
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bioRxiv - Biochemistry 2024Quote: ... and human CNS tissues were extracted with RIPA lysis buffer (Millipore) containing the protease/phosphatase inhibitor cocktail (Thermo Fisher) ...
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bioRxiv - Biochemistry 2024Quote: 16HBE14o- (human bronchial airway epithelial cells) were purchased from EMD Millipore Corporation (Temecula ...
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bioRxiv - Immunology 2024Quote: ... 4 μl/ml of human fibronectin (1 mg/ml; Sigma-Aldrich), and 10 μl of 1 N NaOH (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2024Quote: ... and FGF (Fibroblast growth factor basic human, Sigma-Aldrich, Cat#SRP6159) at 37°C ...
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bioRxiv - Neuroscience 2022Quote: ... and triethyl amine were made in MilliQ ultrapure water (18 megaohm resistance) and 1-octen-3-ol was diluted in paraffin oil (EMD Millipore #PX0045-3). Aliquots of 10 µL of each compound and dilution was pipetted onto a piece of filter paper (5 × 20 mm ...
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bioRxiv - Molecular Biology 2019Quote: ... Cells were then washed 3 times with PBS and incubated with an anti-active Caspase-3 antibody (EMD Millipore, Billerica, MA, 1:200) for 2h at room temperature ...
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bioRxiv - Bioengineering 2021Quote: ... they were anaesthetized in 3-amino benzoic acid ethyl ester (Tricaine/ethyl 3-aminobenzoate; Sigma Aldrich; 168 μg·ml−1 in Tris pH 7) and embedded in 1% low melting point agarose (UltraPure Agarose ...
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bioRxiv - Neuroscience 2020Quote: ... N- (Piperidin-1-yl)-5- (4-iodophenyl)-1- (2,4-dichlorophenyl)-4-methyl-1H-pyrazole-3-carboxamide (AM251, 3 mg/kg; Sigma Aldrich, St. Louis, MO), or vehicle (VEH ...
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bioRxiv - Genetics 2021Quote: ... Mouse neural progenitor cells were routinely passaged 1:2-1:4 every 3-5 days using Accutase and maintained in NS expansion medium on laminin-coated plates (Sigma, 3 μg/ml). Both mESCs and mNPCs were incubated at 37 °C and 5% CO2 ...
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bioRxiv - Genetics 2020Quote: ... containing either tunicamycin only (Enzo Life-Sciences; 2-3 μg/mL) or tunicamycin and auxin (IAA, indole-3-acetic acid, Sigma-Aldrich; 1 mM), and seeded with OP50-1 bacteria ...
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bioRxiv - Microbiology 2022Quote: ... and then transferred to medium differing by the absence of histidine (his) and the presence of 5 mM 3-amino-1,2,4-triazole (3-AT; Sigma-Aldrich, Saint-Louis, MO, USA). Homologous recombination between pPC86 and the PCR product then reconstituted the expression cassette encoding the AD-fused prey protein ...
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bioRxiv - Neuroscience 2024Quote: ... Tissue was mounted on charged slides and dried prior to ethanol washes (70%, 80%, 95%, 100% Ethanol, 3 minutes each) and washed in a histological clearing agent (Xylene substitute; Sigma Aldrich; 3 minutes), and then cover slipped with DPX mounting agent (Sigma Aldrich).
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bioRxiv - Neuroscience 2023Quote: ... The excess fluorescent probe was removed through molecular weight and the buffer was exchanged with PBS (pH 7.4) using 3 kDa centrifugal filter units (UFC500396, Amicon® Ultra – 0.5mL, 3 kDa, Sigma-Aldrich®, Dublin, Ireland). Absorbance was measured at 555 and 280 nm for all tagged samples and calculations were performed according to manufacturer’s instructions using an arbitrary extinction coefficient of 100,000 and molecular mass of 100,000 SI unit ...
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bioRxiv - Biophysics 2022Quote: ... SPR2 DNA encoding residues 649-864 was generated using the polymerase chain reaction method (primers: 5’-GGCAGGACCCATATGGGCAGGAGAGGGTGGGATAATAAAGC-3’ and 5’-GCCGAGCCTGAATTCTTACTTGTCGAACTGTTGGAGATCGATTTC-3’) and individually sub-cloned into pET28 (Millipore Sigma, Burlington, MA) using engineered NdeI and EcoRI restriction endonuclease sites ...
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bioRxiv - Microbiology 2024Quote: ... The chromatic mutants were mixed in different ratios (1:1, 1:3, 3:1) and the mixtures were fixed using 4% paraformaldehyde (Sigma−Aldrich/Merck, Germany) for 4 h at 4°C.
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bioRxiv - Immunology 2022Quote: ... pH 8.0 to a final concentration of 0.2nM and PCR amplified for cloning into T7 phage vector arms (Novagen/EMD Millipore Inc. T7 Select 10-3 Cloning kit). Detailed protocol can be found in dx.doi.org/10.17504/protocols.io.j8nlkkrr5l5r/v1 ...
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bioRxiv - Cell Biology 2023Quote: ... ∼1 kb 5’ homology arm and ∼1 kb 3’ homology arm of msps genomic region were amplified from the genomic DNA (Sigma, Extract-N-Amp™ Tissue PCR Kit), mutated to be insensitive to gRNAs ...
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bioRxiv - Cell Biology 2020Quote: ... or cellulose solution in N-hydroxysuccinimide (NHS; Aldrich; 100 mM) and 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDC, Sigma; 100 mM, 90 min, RT)44 ...
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bioRxiv - Microbiology 2019Quote: ... 5α-androstan-17β-ol-3-one (=17β-hydroxyandrostan-3-one) and 3aα-H-4α(3’-propanoate)-7aβ-methylhexahydro-1,5-indanedione (HIP) were obtained from Sigma-Aldrich (St. Louis, MO, USA). 5α-androstan-3β,17β-diol (=3β,17β-dihydroxyandrostane ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... peptides (<3 kDa) were separated from high molecular size proteins by a 3 kDa cutoff ultrafiltration column (Amicon®, Merck Millipore Ltd., Cork, Ireland). The crude peptides (<3 kDa ...
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bioRxiv - Immunology 2021Quote: The livers of freshly-sacrificed mice were perfused retrogradely via the IVC(3) with 3 ml of PBS and then 10 ml of 2% paraformaldehyde (Sigma, catalogue# 30525-89-4) in PBS ...
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bioRxiv - Immunology 2020Quote: ... injection of 106 hemocytes from late third instar larvae of the lethal(3)malignant blood neoplasm [l(3)mbn1] mutant larvae in Drosophila Ringer’s solution (Sigma-Aldrich, St. Louis, MI, USA). Booster injections were given 4 ...
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bioRxiv - Molecular Biology 2023Quote: ... The expression of 3 × FLAG-tagged MpRBOHB proteins was confirmed by western blot analysis using an anti-3 × FLAG antibody (Sigma-Aldrich, Cat. No. F1804).
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bioRxiv - Biophysics 2023Quote: ... The surface of the coverslips were then coated with a solution of 3% (v/v) (3-Aminopropyl) triethoxysilane (APTES, Sigma-Aldrich, St. Louis, MO) in ethanol for 20 min at room temperature ...