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Citations for Millipore Sigma :
3051 - 3100 of 10000+ citations for NKX2 4 Blocking Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2021Quote: ... were labelled by calcitonin gene-related peptide (CGRP) immunoreactivity using a monoclonal anti-CGRP antibody (1:5000; Sigma #C7113) raised in mouse ...
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bioRxiv - Immunology 2021Quote: ... peptide cocktails were combined with membrane disrupting antimicrobials effective against relevant pathogens beginning at: 10 μM Cecropin A (Sigma), 500μg/mL ampicillin ...
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bioRxiv - Molecular Biology 2022Quote: ... RNCs were eluted after 3 sequential 10 min incubations at room temperature in the RNC buffer that contained 0.2 mg/mL 3x FLAG peptide (Sigma). The elutions were combined and centrifuged at 186,000 x g at 4 °C for 1.5 h in a (TLA-120.1 ...
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bioRxiv - Developmental Biology 2022Quote: ... Beads were then washed three times with lysis buffer and eluted in lysis buffer supplemented with 0.5mg/mL 3xFLAG peptide (Sigma). Eluted proteins were precipitated by adding 20% TCA followed by overnight incubation on ice ...
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bioRxiv - Cell Biology 2022Quote: ... The beads were washed with IP lysis buffer and incubated with 5 mg ml−1 3× FLAG peptide (Sigma–Aldrich ...
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bioRxiv - Immunology 2022Quote: ... at a concentration of 1.5 μg/ml/peptide at 37 °C for 16-18 h in pre-coated ELISpot plate (MSIPS4W10 from Millipore) coated with AN18 IFNγ (1 µg/ml ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... A 20 μL sample of the supernatant and 1 μL of internal peptide standard (H2016, Sigma-Aldrich, Oakville, ON) were added to a 2 mL screw threaded HPLC vial (Chromatographic Specialties ...
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bioRxiv - Plant Biology 2023Quote: ... The washed beads were resuspended in 300 μL wash buffer containing 300 ng/μL of 3xFLAG peptide (F4799, Sigma) and 20 U/mL RNAsin (Promega ...
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bioRxiv - Immunology 2022Quote: ... and 175,000 were co-cultured with 25,000 OTII T cells with 200 ng/mL OVA323-339 peptide (Sigma-Aldrich) and varying ratios of Tregs ...
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bioRxiv - Neuroscience 2022Quote: ... was used in a concentration of 20 µM and autocamtide-2 related inhibitory peptide II (AIP-II; Merck Millipore) at 4 µM.
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bioRxiv - Developmental Biology 2022Quote: ... and peptide peak area ratios were generated against a spiked internal standard with bovine insulin (Sigma – 11070-73-8).
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bioRxiv - Molecular Biology 2024Quote: ... and the FANCI-FANCD2 complex was eluted with FLAG wash buffer containing 100 µg/mL 3X FLAG peptide (Sigma) and 5% glycerol ...
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bioRxiv - Microbiology 2024Quote: ... The antigen-bound magnetic beads were washed five times with lysis buffer and eluted with 2.5 volumes of 3× FLAG peptide (150 ng/μl, Sigma) for 3 h at 4°C with gently shaking ...
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bioRxiv - Plant Biology 2023Quote: ... TF complexes were eluted with 100 μl IP buffer supplemented with 200 μg/ml 3xFLAG peptide (Merck Millipore F4799). The eluted protein was then immobilized on anti-c-Myc magnetic beads (Thermo Fisher 88843 ...
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Cancer-associated fibroblasts produce matrix-bound vesicles that influence endothelial cell functionbioRxiv - Cancer Biology 2023Quote: ... dried peptides were resuspended in 200 mM ammonium formate adjusted to pH 10 with ammonium hydroxide solution (Sigma-Aldrich). Then ...
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bioRxiv - Molecular Biology 2023Quote: ... The pulldowns were eluted from the FLAG and Strep resins using 0.1 mg/mL 3xFLAG peptide (GlpBio) or 2.5 mM desthiobiotin (Sigma-Aldrich), respectively ...
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bioRxiv - Cell Biology 2023Quote: ... The resin was then washed with 3x10 bed volumes of lysis buffer and eluted with 800 µL (for 60 ml of cell suspension) lysis buffer (without protease inhibitor) supplemented with 0.2 mg/mL 3x Flag peptides (Sigma). The N-terminal 3xFlag tag was removed by incubation with the GST tagged Prescission Protease (GenScript ...
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bioRxiv - Molecular Biology 2023Quote: ... and 100 µM Na3VO4) before the protein was eluted with FLAG wash buffer containing 0.5 mg/mL 3xFLAG peptide (Sigma). LIMK1 concentration was determined by running alongside BSA standards on SDS-PAGE with Coomassie staining.
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bioRxiv - Molecular Biology 2023Quote: The FLAG-tagged peptide and Pgk1 were detected using the anti-FLAG M2 (Sigma; cat# F1365; 1:1000 dilution) and anti-Pgk1 22C5D8 (abcam ...
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bioRxiv - Biochemistry 2023Quote: ... with molar ratio of 1:5 (Nucleosome:SUV420H1) or 1:100 (Nucleosome: SUV420H1 C-terminal peptide) in the presence of 40 µM of SAM (Sigma). Samples were incubated on ice for 20 mins ...
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bioRxiv - Biophysics 2023Quote: ... The beads were washed 3 × 1 mL with membrane resuspension buffer for KRas before the beads were eluted with membrane resuspension buffer supplemented with 0.2 mg/mL 3X FLAG peptide (SAE0194, Sigma). Aliquots of samples were collected at each stage (input to anti-FLAG resin ...
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bioRxiv - Cell Biology 2023Quote: ... and FLAG-Sox4 protein was eluted by 5 rounds of competitive elution with 1× column volume (0.6 ml) of 100 μg/ml FLAG peptide (Sigma) in TBS ...
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bioRxiv - Biochemistry 2023Quote: ... The beads were washed three times with buffer E and then mixed with 60 μg of 3xFLAG peptide (SIGMA) in 60 μl of buffer E for 1 h with shaking at 1400 rpm at 4°C ...
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bioRxiv - Biochemistry 2023Quote: ... Proteins were eluted with 200 µL assay buffer supplemented with 0.6 mg∕mL 3× Flag® peptide (Sigma Aldrich), and analysed by Western blotting.
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bioRxiv - Biochemistry 2023Quote: ... dried peptides were dissolved in 20 μl of 2% formic acid supplemented with 20 mM citric acid (Sigma-Aldrich). Subsequently ...
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bioRxiv - Molecular Biology 2023Quote: ... the protein complex was eluted with 500 μL elution buffer (0.4 mg/ml 3X Flag peptide (Millipore Sigma F4799) in wash buffer ...
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bioRxiv - Biochemistry 2024Quote: ... The target protein was eluted twice by incubating the matrix with 0.5 mL of K buffer supplemented with 200 μg/mL FLAG peptide (Sigma) for 0.5 h ...
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bioRxiv - Cell Biology 2024Quote: ... The obtained beads fraction was resuspended with 180 μl of lysis buffer containing Influenza Hemagglutinin (HA) -peptide (Sigma-Aldrich) at the final concentration of 0.2 mg/ml ...
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bioRxiv - Immunology 2024Quote: ... T2 cells were incubated with a titration of each tested peptide and 100 ng/mL human β2-microglobulin (Sigma) in serum-free medium (TexMacs ...
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bioRxiv - Molecular Biology 2024Quote: ... The column was then washed 10 times with 1x Polymix buffer before elution in 200 µl of 1x Polymix supplemented with 0.1 mg/mL FLAG3 peptide (Sigma). 200 µl of the input lysate was saved and incubated at 4°C to use as a control for 5PSeq analysis of pulldown samples ...
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bioRxiv - Developmental Biology 2021Quote: ... At the relevant timepoint embryos were fixed overnight at 4°C using 4 % (w/v) paraformaldehyde (Sigma-Aldrich, UK) in phosphate buffered saline (PBS) ...
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bioRxiv - Evolutionary Biology 2021Quote: ... 0.1% w/v Ferrozine (Disodium-4-[3-pyridin-2-yl-6-(4-sulfonatophenyl)-1,2,4-triazin-5-yl]benzosulfonate (Sigma-Aldrich) in dd ...
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bioRxiv - Cell Biology 2022Quote: ... DNA damage was induced where indicated by treatment with 2 μg/ml 4-Nitroquinoline 1-oxide (4-NQO) (Sigma) for 60 min before harvesting ...
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bioRxiv - Molecular Biology 2019Quote: ... For the first 5 days of remyelination the mice were administered 4-hydroxytamoxifen (4-HT, 1mg/day/mouse, Sigma) as previously described (Baxi ...
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bioRxiv - Biochemistry 2019Quote: Inhibition of WT LcαE7 and the Gly137Asp LcαE7 variant was determined by a competition assay between the native-substrate analogue 4-nitrophenol butyrate (4-NPB, Sigma) and the boronic acid compounds ...
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bioRxiv - Biochemistry 2020Quote: ... 7-amido-4-methyl-coumarin (NHMec) from the commercially available substrate Leucine-7-amido-4-methyl-coumarin (Sigma-Aldrich). For processivity assays ...
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bioRxiv - Cancer Biology 2020Quote: ... Cells were fixed in 4% methanol-free formaldehyde (Thermo, 28906) and permeabilized in .4% Triton X-100 (Sigma, T8787). The Click-iT Cell Reaction Buffer Kit was then used to “click” the biotin alkyne (Thermo ...
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bioRxiv - Cell Biology 2021Quote: ... the cell culture media was replaced with fresh media and supplemented with 1μM 4-OHT (4-hydroxytamoxifen, Sigma, H7904) for both control and mutant cells ...
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bioRxiv - Cell Biology 2022Quote: ... Cre-mediated recombination was induced in embryos by intraperitoneal injection of 0.5 mg (Cdh5-CreERT2) or 2 mg (Vegfr1-CreERT2, Vegfr3-CreERT2) of 4-hydroxytamoxifen (4-OHT, H7904, Sigma), dissolved in peanut oil (10 mg/ml) ...
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bioRxiv - Neuroscience 2021Quote: ... the mice were given an intraperitoneal injection of 10 mg kg-1 of 4-hydroxytamoxifen (4-OHT; Sigma, H6278) dissolved in a 1:4 mixture of castor oil (Sigma ...
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bioRxiv - Immunology 2019Quote: ... mouse peritoneal neutrophils were harvested 4 h after intraperitoneal injection of 2 ml of 4% thioglycollate broth (Sigma-Aldrich). The collected peritoneal neutrophils were cultured in RPMI 1640 (Welgene ...
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bioRxiv - Neuroscience 2020Quote: ... tadpoles were euthanized using MS-222 (1 g/L) and fixed in 4% paraformaldehyde (Sigma - CAS 30525-89-4) for 3 h at room temperature (RT) ...
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bioRxiv - Plant Biology 2021Quote: Around 750 entire-4 (e-4) seeds were treated with the mutagenic substance Ethyl-Methane Sulfonate (EMS, Sigma m0880) at a concentration of 0.6% for 10 hours ...
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bioRxiv - Cell Biology 2021Quote: β-catenin knock-out in βfl/- cells was induced through the addition of 0.1 μg/mL 4-hydroxy-tamoxifen (4-HT, cat. num. H6278, Sigma) to the media every 24 hours for 72 hours ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: Male C57Bl/6J mice were treated with 5% 4-MU (Alpha Aesar) and 2 mg/ml 4-MUG (Sigma) starting at 8 weeks of age for 20 days ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Male C57Bl/6J mice were treated with 5% 4-MU (Alpha Aesar) and 2 mg/ml 4-MUG (Sigma) starting at 8 weeks of age and maintained on this diet until they were euthanized ...
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bioRxiv - Neuroscience 2022Quote: ... data was separated into pre-defined frequency bins (Delta 0.5-4 Hz, Theta 4-8 Hz, Alpha 8-13 Hz, Sigma 12-15 Hz ...
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bioRxiv - Neuroscience 2023Quote: ... and the sample was incubated for 1.5h rotating at 4°C before the addition of 1/4 volume of 3.06% cycloamylose (Sigma-Aldrich). The sample was incubated overnight rotating at 4°C ...
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bioRxiv - Cell Biology 2023Quote: ... and fumaric acid-1,4-13C2,2,3-2H2 (≥99 atom% 13C, ≥98 atom %2H, CAS 100858-52-4, cat. no. 752576) from Sigma Aldrich. All chemicals were used as received.
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bioRxiv - Genetics 2023Quote: ... embryos were fixed in 4% paraformaldehyde overnight at 4°C, washed in PBST (1× PBS, 0.1% Tween 20 (Sigma)) ...