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Citations for Millipore Sigma :
3301 - 3350 of 10000+ citations for NKX2 4 Blocking Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2023Quote: ... Affinity purified FH-Ago2 bound with miRNA (most population bound with miR-122 due to its overexpression) was eluted from anti-FLAG beads by competing with 3X FLAG peptide (Sigma) as per the manufacturer’s protocol in a purification buffer (30mM HEPES pH 7.4 ...
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bioRxiv - Plant Biology 2023Quote: ... A binary gradient was used for peptide elution, where mobile phase A consisted of water (Tedia, Fairfield, Ohio, USA) with 0.1% formic acid (Sigma-Aldrich), and mobile phase B consisted of acetonitrile (Sigma-Aldrich ...
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bioRxiv - Molecular Biology 2023Quote: ... we cleaved the peptides from beads with standard TFA cleavage cocktail (comprising 95% TFA, 2.5% water and 2.5% Triisopropylsilane (Sigma, Cat #233781) for 2-4 hours at room temperature ...
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bioRxiv - Molecular Biology 2023Quote: ... Reh1-bound ribosomes were eluted in 100 ul of IP buffer supplemented with 0.1% Igepal and 150 ug/ml 3xFLAG peptide (Sigma Milipore) for 15 min at 4°C ...
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bioRxiv - Immunology 2023Quote: ... beads were washed with IP buffer thrice and the bead bound FLAG-HA-STX5/truncated STX5 was eluted using 3x FLAG peptide (Sigma) as per the manufacturer’s instructions in a purification buffer (30 mM HEPES pH 7.4 ...
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bioRxiv - Immunology 2023Quote: ... Samples were then washed with lysis buffer containing 0.1% DDM and incubated with lysis buffer containing 1% DDM and 3×Flag peptide (100 µg/ml, Sigma #F4799) overnight for elution ...
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bioRxiv - Cell Biology 2023Quote: ... were fused with mNeongreen at their C-terminus with a flexible peptide linker and cloned into pET28a vector (Millipore; 69864). Protein expression in Escherichia coli BL21(DE3 ...
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bioRxiv - Microbiology 2024Quote: ... The protein was finally eluted with 50 μL of 300 ng/μL 3x FLAG peptide (Sigma-Aldrich, St. Louis, MO) for 45 minutes at 4°C ...
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bioRxiv - Biophysics 2024Quote: ... NEDD4-2 HECT domain was subsequently isolated from the supernatant with anti-FLAG antibody (M2) affinity chromatography and eluted with 100 μg/mL FLAG peptide (Sigma Millipore) in 50 mM TrisHCl ...
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bioRxiv - Immunology 2024Quote: ... of each of the 32 CC strains were immunized subcutaneously with 200 µg MOG35-55 (New England Peptide) emulsified in CFA (Sigma), supplemented with additional 4 mg/ml heat-killed Mycobacterium tuberculosis (Difco Laboratories) ...
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bioRxiv - Immunology 2024Quote: ... T2 cells were pulsed overnight at 37°C with 100 μM of individual peptides in serum-free medium (TexMacs) supplemented with 100 ng/µL of human β2-microglobulin (Sigma), followed by the indicated chase kinetics performed in the presence of Brefeldin A (Sigma ...
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bioRxiv - Molecular Biology 2024Quote: ... Beads were then washed four times with cold lysis buffer and immune complexes were eluted with 75 μL of 3XFLAG peptide (Sigma) (200 mg/mL ...
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bioRxiv - Plant Biology 2024Quote: ... Samples were digested by using trypsin at 10 ng/µL at 37°C overnight and peptides were harvested by with 50% ACN/0.1% FA (formic acid, Sigma #06473), lyophilized and resuspended with 0.1% FA ...
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bioRxiv - Genetics 2024Quote: ... cOmplete proteinase inhibitor) to remove nonspecific-binding proteins and then HA-tagged proteins were eluted with HA peptides (Sigma-Aldrich). WT samples (not HA tagged ...
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bioRxiv - Developmental Biology 2020Quote: ... Ovary of both medaka and trout were fixed overnight at 4°C by 4% paraformaldehyde (PFA) in 0,01 M phosphate buffer saline pH 7.4 (PBS, Sigma-Aldrich P4417). Ovaries were washed in PBS and conserved in PBS + 0.5% (w/v ...
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bioRxiv - Neuroscience 2021Quote: ... DRGs were centrifuged for 4 min at 500 xg at 4°C and incubated in 1 ml dissociation buffer (2.5mg/ml collagenase, Sigma Aldrich, C9722 ...
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bioRxiv - Neuroscience 2020Quote: ... followed by 4% paraformaldehyde in 0.1 M phosphate buffer (4% PFA-PB; pH 7.4; Sigma-Aldrich, St Louis, MO, USA). Lumbar spinal cords were collected and placed into 4% PFA-PB for 2 hours at 4 °C ...
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bioRxiv - Systems Biology 2020Quote: ... and ddH2O and stained for cell volume with 12.5 μg/ml bis(2,2’-bipyridine)-4’-methyl-4-carboxybipyridine-ruthenium-N-succidimyl ester-bis(hexafluorophosphate) (96Ru, 98-102Ru, 104Ru, Sigma Aldrich) in 0.1 M sodium hydrogen carbonate ...
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bioRxiv - Cancer Biology 2019Quote: ... The endocrine-resistant cells were kept in phenol-red free medium supplemented with 10% heat-inactivated charcoal-stripped-FBS and 1% P/S with the addition of 100 nM 4-hydroxytamoxifen (4-OHT, H7904, Sigma). All cells were kept at 37°C in a humified incubator with 5% CO2.
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... SAG1.3 (3-Chloro-N-[trans-4-(methylamino)cyclohexyl]-N-[[3-(4-pyridinyl)phenyl]methyl]benzo[b]thiophene-2-carboxamide) was from Sigma. Cyclopamine-KAAD and SANT-1 ((4-Benzyl-piperazin-1-yl)-(3,5-dimethyl-1-phenyl-1H-pyrazol-4-ylmethylene)-amine ...
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bioRxiv - Developmental Biology 2020Quote: ... Sma-CreERT2 or Cx40-CreERT2 males were crossed with R26-Confetti females and low doses of 4-Hydroxytamoxifen (4-OHT H6278, Sigma) was injected intraperitoneally to pregnant females in a single injection ...
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bioRxiv - Biochemistry 2021Quote: ... and 4) the substrate solution (nitro blue tetrazolium/5-bromo-4-chloro-3-indolyl phosphate, Sigma-Aldrich, St. Louis, MO). Other details were as described previously [Duk M. ...
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bioRxiv - Neuroscience 2021Quote: ... Cells were subsequently fixed in paraformaldehyde (PFA) 4% with 4% sucrose for 10 min and permeabilized with 0,1% Triton X100 (Sigma-Aldrich) for 5 min ...
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bioRxiv - Neuroscience 2020Quote: 4% hydrogel monomer solution (HMS) was prepared for perfusion by mixing 40% (w/v) acrylamide (4% final concentration; V900845, Sigma), 2% (w/v ...
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bioRxiv - Neuroscience 2020Quote: ... Neurons were incubated at 37°C for 30 minutes prior to fixation with 4% PFA (Electron Microscopy Services) containing 4% sucrose (Sigma) for 30 min at 37°C ...
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bioRxiv - Biochemistry 2020Quote: ... Extracted membranes were separated from insoluble debris by centrifugation (130,000 × g for 1 h at 4 °C) and incubated for 2h with 4 mL of Ni2+-NTA beads (Novagen) equilibrated in buffer III (25 mM Tris-HCl ...
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bioRxiv - Neuroscience 2020Quote: Cells were washed with phosphate buffered saline (PBS) and fixed for 30 min at room temperature with 4% paraformaldehyde (containing 4% sucrose; Sigma). Cells were permeabilized in 0.5% NP40 in PBS for 5 min at room temperature ...
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bioRxiv - Microbiology 2020Quote: ... and 0.26g (12.48 x 10−4 mol, 1:4 stoichiometric ratio) cross-linking agent tetraethyl orthosilicate (TEOS – Sigma-Aldrich, 131903) were mixed at 3500rpm for 1 min using a DAC 150FV2-K speedmixer ...
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bioRxiv - Cell Biology 2021Quote: ... Chromatin was pelleted by spinning 5 minutes at 10,000 g at 4°C and subsequently incubated for 1 hour at 4°C in HEPES buffer containing 500 units of Benzonase (Millipore) and 2 µg Pol II antibody (ab5095 ...
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bioRxiv - Cell Biology 2020Quote: ... Samples were loaded onto self-made 4–13% or 4-10% polyacrylamide gradient gels separated and transferred onto PFDV membranes (Merck Millipore) followed by detection by specific antibodies ...
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bioRxiv - Microbiology 2022Quote: ... Samples were centrifuged 1800 rpm for 6 minutes at 4°C and pellets were resuspended n 3 mL RBC lysis for 4 minutes (Sigma). Samples were centrifuged 1800 rpm for 6 minutes at 4°C and the pellets were resuspended in 200 μl of PBS + 2% FBS + 2 mM EDTA ...
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bioRxiv - Neuroscience 2020Quote: ... NestinCreERT2 - /+ TDT +/+ IR fl/fl generated secondary spheres were allowed to grow for 4 DIV and then 0.5 µM of 4-hydroxy tamoxifen (Sigma-Aldrich) or vehicle (ethanol ...
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bioRxiv - Neuroscience 2021Quote: ... in either 4-well plates or P35 dishes (ThermoScientific) precoated for 4 h with 50 μg/ml poly-D-lysine (Sigma). Neurons were plated in DMEM containing 10 unit/mL penicillin ...
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bioRxiv - Neuroscience 2020Quote: ... Induction of recombination in Ai9 mice electroporated with Cre inducible plasmids was achieved by intraperitoneal injection of pregnant dams with the faster-acting and shorter-lived tamoxifen metabolite 4-hydroxy-tamoxifentamoxifen (4-OHT) (Sigma) (1 mg/20 g of body weight ...
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A critical role for heme synthesis and succinate in the regulation of pluripotent states transitionsbioRxiv - Developmental Biology 2022Quote: Cells were seeded on coated glass cover (Assistent) slips 2 days before fixation with 4 % paraformaldehyde (Sigma, 30525-89-4) for 15 minutes ...
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bioRxiv - Immunology 2022Quote: ... 0.6μL was deposited on a thin layer of an air-dried saturated solution (0.6μL) of the matrix alpha-cyano-4-hydroxycinnamic Acid (4-HCCA, Sigma Aldrich, France) in pure acetone ...
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bioRxiv - Biochemistry 2019Quote: ... The selected fractions of the SEC peak corresponding to the purified Dispatched were concentrated to 4 mg/ml using an Amicon Ultra-4 concentrator (100 kDa cut-off, Millipore).
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bioRxiv - Microbiology 2019Quote: ... aliquots of 100 μl of the supernatants were mixed with an equal volume of 4 % w/v Ehrlich’s reagent 4-(dimethylamino) benzaldehyde (156477, Sigma-Aldrich) in glacial acetic acid (Fisher Chemical A/0360/PB15) ...
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bioRxiv - Cancer Biology 2019Quote: ... The vDISCO pipeline was started immediately or whole mouse bodies were stored in PBS at 4 °C for up to 4 weeks or in PBS containing 0.05% sodium azide (Sigma, 71290) for up to 6 months.
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bioRxiv - Microbiology 2019Quote: ... The cells were cultured for 24 hrs post transfection before addition of fresh media containing 100 µM 4-thiouridine (4-SU, Sigma), a photo-crosslinkable ribonucleoside analogue ...
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bioRxiv - Microbiology 2020Quote: ... and the resulting adhered bacteria on the cover slip were stained with 200 μL of the membrane stain N-(3-Triethylammoniumpropyl)-4-(6-(4-(Diethylamino) Phenyl) Hexatrienyl) Pyridinium Dibromide (FM4-64) (Sigma) at a final concentration of 20 μg/mL for 5 minutes ...
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bioRxiv - Bioengineering 2020Quote: ... the brain was kept in 4% PFA overnight at 4□°C and then placed into PBS containing 0.02% sodium azide (Sigma S8032). The brain was serially sectioned into 50 μm transverse slices on a Campden Instruments 5100mz vibratome ...
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bioRxiv - Cell Biology 2021Quote: ... cells were stained with 12.5 μg/ml Bis(2,2′-bipyridine)-4′-methyl-4-carboxybipyridine-ruthenium-N-succidimyl ester-bis(hexafluorophos-phate) (96Ru, 98-102Ru, 104Ru, Sigma Aldrich) in 0.1 M sodium hydrogen carbonate (Sigma Aldrich ...
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bioRxiv - Synthetic Biology 2020Quote: ... the supernatant was collected and then 4 fractions of 4mL were concentrated 8 times using 10 kDa Amicon Ultra-4 10K Centrifugal filters (Millipore) after centrifugation (20 min ...
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bioRxiv - Microbiology 2020Quote: ... Flagella preparations were incubated at 70°C for 15 min then filtered by centrifugation at 5000 × g at 4°C for 30 min using 100 kDa 4 ml filter units (Millipore). Filtrates were confirmed by size-exclusion HPLC to be exclusively monomeric.
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bioRxiv - Microbiology 2021Quote: ... GM130 (12480, cell signaling), PI(4)P (Z-P004), PI(4)KB (06-578, Milipore) and anti-actin (A5316, Sigma). Secondary antibodies were as followed ...
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bioRxiv - Neuroscience 2020Quote: ... Srff/f and Srff/fCaMKCreERT2 pups were intraperitoneally injected every other day with three doses of 0.25 mg 4-hydroxytamoxifen (4-OHT; Sigma, #7904) or one dose of 0.75 mg of this compound in sunflower oil ...
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bioRxiv - Genetics 2020Quote: ... and the colour reaction was carried out with NBT/BCIP (4-nitroblue tetrazolium chloride/bromo-4-chloro-3-indolyl phosphate; Sigma). Sections were dehydrated and mounted with Vectamount (Vector Laboratories ...
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bioRxiv - Immunology 2021Quote: PBMCs and tissue MNC suspensions were treated with 5 μM Chk2 inhibitor II or 2-[4-(4-chlorophenoxy)phenyl]-1H-benzimidazole-5-carboxamide (Sigma) prepared in final volume of 1% bovine serum albumin (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2021Quote: Oocyte live cell imaging was done in 4-well Labtek chambers (ref. 155383) in 4 μl drops of M16 medium covered with mineral oil (Sigma) in a 5% CO2 environmental microscope incubator at 37 °C (Pecon) ...