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Citations for Millipore Sigma :
3001 - 3050 of 10000+ citations for Integrin alpha 4 Rabbit Recombinant mAb since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2019Quote: ... using a VP-ITC microcalorimeter (MicroCal) as described [34] after extensive dialysis of the purified recombinant protein in Chelex 100 (Sigma) to remove contaminating divalent cations ...
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bioRxiv - Developmental Biology 2020Quote: ... The trophectoderm was removed by immunosurgery and “epiblasts” were grown in gelatinised dishes in the presence of NDiff + 2i and ESGRO (recombinant mouse LIF Protein, Millipore) to establish ESC lines.
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bioRxiv - Cancer Biology 2021Quote: Primary HPMECs in EBM2 medium or ST1.6R cells in DMEM/1% FBS were cultured overnight and stimulated with 2 μg/ml of recombinant TNC (Merck Millipore) for 24 h ...
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bioRxiv - Cell Biology 2019Quote: ... Immune sera were generated by immunization of 6 weeks-old female NMRI mice (Charles Liver Laboratories) via subcutaneous injection of 100 µg recombinant protein emulsified in Freund’s incomplete adjuvant (Sigma-Aldrich) followed by a boost after 4 weeks with 50 µg of recombinant protein ...
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bioRxiv - Biochemistry 2019Quote: ... STAT1 (0.3 µM) (Biosource, PHF0011) was phosphorylated in vitro by incubation with recombinant human cdk1/cyclinB (0.2 µM) (Millipore, 14-450) at 30 °C for 90 min in the presence of 1 mM ATP ...
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bioRxiv - Biochemistry 2019Quote: ... Proteins were digested for at least 14 h (overnight) with an animal-free recombinant bovine trypsin expressed in corn (Trypzean T3568 from Sigma) at 1/50 w/w and RT ...
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bioRxiv - Bioengineering 2020Quote: ... incubated 30 seconds in plasma cleaner then coated with cell-specific adhesive surface: for at least 1 hour with 10 μg/ml recombinant human fibronectin (Sigma) for Hela and SHSy-5Y cells ...
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bioRxiv - Microbiology 2021Quote: ... kinetics was recorded as the HA loaded sensors were dipped into 1.5-fold serially diluted concentrations of recombinant GM2 (Sigma Aldrich). The reaction was carried out in a 1x kinetics buffer comprising of 1x PBS ...
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bioRxiv - Physiology 2020Quote: ... tau/NTF or N-terminal (243-441) tau/CTF cDNA was used to produce recombinant tau protein in Escherichia coli BL21 (DE3) (Merck Millipore). Purification was performed as described previously (Hasegawa et al. ...
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bioRxiv - Biophysics 2021Quote: Catalytic activity was evaluated in vitro using 20 pmol of purified recombinant proteins in a 200 µl reaction buffer supplemented with 20 mM p-nitrophenyl phosphate (Sigma) as substrate ...
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bioRxiv - Microbiology 2021Quote: ... Purification of the recombinant mucoricin was performed by Ni-NTA matrix affinity purification according to the manufacturers’ instructions (Sigma-Aldrich). The purity of the protein was confirmed by SDS-PAGE and quantified by a modified Lowry protein assay (Pierce).
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bioRxiv - Molecular Biology 2022Quote: ... and 1 μg of FhNEJ-Teg or recombinant protein in the presence or absence of 15 ng t-PA (Sigma) or 10 ng u-PA (Sigma) ...
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bioRxiv - Neuroscience 2023Quote: ... we randomly assigned Ai9:Tmem119cre mice to receive an intravenous injection of control solution or 0.05 mg/kg of recombinant IFNγ from mouse (Sigma #I4777), minutes before initiating the rupture of cortical capillaries and then 12h later ...
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bioRxiv - Immunology 2022Quote: ... expanded in the presence of recombinant human IL-2 (1000 U/mL, Proleukin) and rapamycin (50 nmol/L, Sigma-Aldrich), and transduced after 2 days ...
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bioRxiv - Cancer Biology 2022Quote: ... PDAC cells were treated with human recombinant FasL (MiaPaCa2: 50 ng/mL; TKCC10: 100 ng/mL) (Sigma-Aldrich, cat. SRP3036), 48 hours post transfection ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Supplemented IVM media for OSC-IVM consisted of IVM media (Medicult, Origio) supplemented with 75 mIU/ml of recombinant human FSH (Millipore), 100 mIU/ml recombinant human chorionic gonadotropin (hCG ...
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bioRxiv - Microbiology 2023Quote: ... 21 and 42 with 50 μg of recombinant LMW630 mixed with 200 ng/mouse pertussis toxin (Sigma-Aldrich, MO, USA). Enzyme-linked immunosorbent assay was performed to measure serum responses to antigen (see methods below ...
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bioRxiv - Molecular Biology 2024Quote: ... Further confirmation of purification of the recombinant protein was obtained by immunoblots probed with mouse anti-polyhistidine IgG (Sigma-Aldrich) and goat anti-mouse alkaline phosphatase-conjugated antibodies (Thermo Fisher Scientific) ...
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bioRxiv - Molecular Biology 2022Quote: ... Jurkat cells were infected with the recombinant viruses in the presence of 8 µg/ml polybrene (hexadimethrine bromide, Sigma-Aldrich) for 15 hours at 37°C ...
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bioRxiv - Microbiology 2023Quote: ... were subcutaneously injected with 100 µg of the pure recombinant protein emulsified in Freund’s incomplete adjuvant (Sigma Aldrich, Taufkirchen, DE) followed by a boost after 4 weeks ...
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bioRxiv - Cell Biology 2022Quote: ... and Mirg-/-littermates injected subcutaneously with Growth factor-reduced Matrigel BD) with 400 ng mL-1 recombinant human bFGF (Millipore). After 7 days Matrigel plugs ...
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bioRxiv - Microbiology 2023Quote: ... All the recombinant viruses were rescued by transfecting BAC DNA into Vero cells using lipofectamine DNA transfection reagent (Millipore-Sigma) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... All the recombinant viruses were rescued by transfecting BAC DNA into Vero cells using lipofectamine DNA transfection reagent (Millipore-Sigma) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... GST-tagged recombinant protein was eluted by three sequential incubations of GSH-beads with 50mM reduced L-GSH (SIGMA, G4251) in 100mM Tris-HCl [pH8.0],150mM NaCl ...
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bioRxiv - Developmental Biology 2024Quote: Bovine OVGP1 (bOVGP1) and murine OVGP1 (mOVGP1) recombinant proteins were purified using the Amicon Pro Purification System (Merck Millipore Ltd) by combining metal chelation chromatography with an Amicon concentrator membrane ...
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bioRxiv - Systems Biology 2024Quote: ... 60µg of total protein per sample and 5µg recombinant Tau (rTau) (2N4RTau and 1N3R Tau) were digested with either Trypsin Protease (Pierce) or LysargiNase (EMD Millipore) at 1:50 w/w overnight at room temperature ...
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bioRxiv - Cancer Biology 2020Quote: ... FBXW7 rabbit monoclonal (SP-237) (Spring Bioscience, CA) and the USP28 rabbit polyclonal (HPA006778) (Sigma Aldrich) antibody staining were performed using the Ventana Bench Mark ULTRA™ automated staining platform and the Optiview™ Detection kit (12) ...
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bioRxiv - Cell Biology 2021Quote: ... anti-ADAM10 rabbit polyclonal and anti-APP C-terminal rabbit polyclonal antibodies were from Sigma-Aldrich Company Ltd ...
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bioRxiv - Pathology 2019Quote: ... rabbit anti-mouse von-Willebrand factor (vWF) and rabbit anti-mouse podocin (Sigma, St. Louis, MO). All secondary IgG class antibodies were from Thermofisher (Waltham ...
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bioRxiv - Neuroscience 2024Quote: ... #L-1516)/1:1000 rabbit/goat anti-PV (Swant, #PV27, #PVG-214)/ rabbit anti-ACAN (Merck Millipore, #ab1031), in block ...
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Juxtacrine DLL4-NOTCH1 signaling between astrocytes drives neuroinflammation via the IL-6-STAT3 axisbioRxiv - Neuroscience 2023Quote: ... Anti-human-DLL4 (Rabbit) and anti-LAM (rabbit) were from Sigma Aldrich (St. Louis, MO, USA). Anti β-ACTIN (rabbit) ...
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bioRxiv - Developmental Biology 2021Quote: ... At the relevant timepoint embryos were fixed overnight at 4°C using 4 % (w/v) paraformaldehyde (Sigma-Aldrich, UK) in phosphate buffered saline (PBS) ...
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bioRxiv - Evolutionary Biology 2021Quote: ... 0.1% w/v Ferrozine (Disodium-4-[3-pyridin-2-yl-6-(4-sulfonatophenyl)-1,2,4-triazin-5-yl]benzosulfonate (Sigma-Aldrich) in dd ...
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bioRxiv - Cell Biology 2022Quote: ... DNA damage was induced where indicated by treatment with 2 μg/ml 4-Nitroquinoline 1-oxide (4-NQO) (Sigma) for 60 min before harvesting ...
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bioRxiv - Molecular Biology 2019Quote: ... For the first 5 days of remyelination the mice were administered 4-hydroxytamoxifen (4-HT, 1mg/day/mouse, Sigma) as previously described (Baxi ...
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bioRxiv - Biochemistry 2019Quote: Inhibition of WT LcαE7 and the Gly137Asp LcαE7 variant was determined by a competition assay between the native-substrate analogue 4-nitrophenol butyrate (4-NPB, Sigma) and the boronic acid compounds ...
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bioRxiv - Biochemistry 2020Quote: ... 7-amido-4-methyl-coumarin (NHMec) from the commercially available substrate Leucine-7-amido-4-methyl-coumarin (Sigma-Aldrich). For processivity assays ...
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bioRxiv - Cancer Biology 2020Quote: ... Cells were fixed in 4% methanol-free formaldehyde (Thermo, 28906) and permeabilized in .4% Triton X-100 (Sigma, T8787). The Click-iT Cell Reaction Buffer Kit was then used to “click” the biotin alkyne (Thermo ...
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bioRxiv - Cell Biology 2021Quote: ... the cell culture media was replaced with fresh media and supplemented with 1μM 4-OHT (4-hydroxytamoxifen, Sigma, H7904) for both control and mutant cells ...
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bioRxiv - Cell Biology 2022Quote: ... Cre-mediated recombination was induced in embryos by intraperitoneal injection of 0.5 mg (Cdh5-CreERT2) or 2 mg (Vegfr1-CreERT2, Vegfr3-CreERT2) of 4-hydroxytamoxifen (4-OHT, H7904, Sigma), dissolved in peanut oil (10 mg/ml) ...
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bioRxiv - Neuroscience 2021Quote: ... the mice were given an intraperitoneal injection of 10 mg kg-1 of 4-hydroxytamoxifen (4-OHT; Sigma, H6278) dissolved in a 1:4 mixture of castor oil (Sigma ...
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bioRxiv - Immunology 2019Quote: ... mouse peritoneal neutrophils were harvested 4 h after intraperitoneal injection of 2 ml of 4% thioglycollate broth (Sigma-Aldrich). The collected peritoneal neutrophils were cultured in RPMI 1640 (Welgene ...
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bioRxiv - Neuroscience 2020Quote: ... tadpoles were euthanized using MS-222 (1 g/L) and fixed in 4% paraformaldehyde (Sigma - CAS 30525-89-4) for 3 h at room temperature (RT) ...
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bioRxiv - Plant Biology 2021Quote: Around 750 entire-4 (e-4) seeds were treated with the mutagenic substance Ethyl-Methane Sulfonate (EMS, Sigma m0880) at a concentration of 0.6% for 10 hours ...
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bioRxiv - Cell Biology 2021Quote: β-catenin knock-out in βfl/- cells was induced through the addition of 0.1 μg/mL 4-hydroxy-tamoxifen (4-HT, cat. num. H6278, Sigma) to the media every 24 hours for 72 hours ...
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bioRxiv - Neuroscience 2023Quote: ... and the sample was incubated for 1.5h rotating at 4°C before the addition of 1/4 volume of 3.06% cycloamylose (Sigma-Aldrich). The sample was incubated overnight rotating at 4°C ...
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bioRxiv - Cell Biology 2023Quote: ... and fumaric acid-1,4-13C2,2,3-2H2 (≥99 atom% 13C, ≥98 atom %2H, CAS 100858-52-4, cat. no. 752576) from Sigma Aldrich. All chemicals were used as received.
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bioRxiv - Genetics 2023Quote: ... embryos were fixed in 4% paraformaldehyde overnight at 4°C, washed in PBST (1× PBS, 0.1% Tween 20 (Sigma)) ...
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bioRxiv - Neuroscience 2024Quote: ... followed by transcardial perfusion with 1X phosphate-buffered saline (PBS) and then 4% paraformaldehyde (Sigma-Aldrich, 30525-89-4) diluted in 1X PBS ...
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bioRxiv - Neuroscience 2024Quote: ... was centrifugally filtered at 9,100g for 4 h at 4 °C through a 5-kDa cut-off filter (Millipore) to remove proteins ...