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Citations for Millipore Sigma :
3001 - 3050 of 10000+ citations for 6 METHOXY 2 METHYL 1 PHENYLSULFONYL 1H INDOLE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... 1 M tris(2-carboxyethyl)phosphine hydrochloride (TCEP, Sigma-Aldrich, C4706), and 0.6 M 2-chloroacetamide (CAM ...
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bioRxiv - Molecular Biology 2024Quote: ... and anti-α-Tubulin (B-5-1-2, Merck Millipore/Sigma) antibodies were purchased ...
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bioRxiv - Neuroscience 2024Quote: ... combined with rabbit anti- MAP-2 (Merck Millipore, #AB5622, 1:800), followed by goat anti-mouse Dylight405 (Jackson ImmunoResearch ...
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bioRxiv - Developmental Biology 2024Quote: ... MPM-2 Mitotic Protein Monoclonal mouse 1:100 (Millipore 05-368), Lamin mouse 1:300 (DSHB ADL67.10) ...
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bioRxiv - Developmental Biology 2024Quote: ... Embryos were incubated in 0.003% 1-phenyl 2-thiourea (Sigma P7629) in E3 embryo media to inhibit pigment synthesis ...
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bioRxiv - Molecular Biology 2024Quote: ... and anti-α-Tubulin (B-5-1-2, Merck Millipore/Sigma) antibodies were purchased ...
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bioRxiv - Neuroscience 2024Quote: ... 2% horse serum (Hyclone SH30074.03) and 1 mM pyruvate (Sigma S8636). The cultures were supplemented with 45 μg/ml of E18 chicken leg extract kept at 37 ℃ and 5% CO2 ...
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bioRxiv - Neuroscience 2024Quote: (-)-1-(2,5-dimethoxy 4-iodophenyl)-2-aminopropane hydrochloride (DOI, Sigma-Aldrich) was and dissolved in sterile water at a concentration of 1mg/mL ...
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bioRxiv - Molecular Biology 2021Quote: ... cells (5-6 x 106 in 10 cm dishes) were cross-linked with 10 ml 1% formaldehyde (Sigma) in medium for 5 min at room temperature and then incubated in 10 ml 200mM glycine in medium for 5 min ...
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bioRxiv - Biophysics 2020Quote: ... sulforhodamine B acid chloride (SRB) and 6-mer-capto-1-hexanol were purchased from Sigma-Aldrich (Taufkirchen, Germany). Atto488 maleimide and Atto655/488/390-1,2-dipalmitoyl-sn-glycero-3-phosphoethanolamine (Atto655/488/390 DPPE ...
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bioRxiv - Plant Biology 2020Quote: ... The symplasmic tracer 5(6)-carboxyfluorescein diacetate (CFDA; 50 mg mL-1 in dimethyl sulfoxide [DMSO]; Sigma, USA) was diluted with sterile water to 500 μg mL-1 to generate a working solution ...
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bioRxiv - Cancer Biology 2022Quote: ... For treatment with chemotherapeutic agents cells were treated with either Doxorubicin 1-6 mg/ml (Sigma, cat #D1515), NCS 1-150ng/ml (Sigma ...
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bioRxiv - Molecular Biology 2020Quote: ... 1 μl of T4 DNA ligase buffer (10x stock) and 6 μl of PEG-4000 (Sigma Aldrich, 50%) per amplicon pool reaction ...
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bioRxiv - Cell Biology 2021Quote: To measure symplasmic connectivity using the HPTS (8-Hydroxypyrene-1, 3, 6-trisulfonic acid trisodium salt, SIGMA-ALDRICH) dye movement assay ...
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bioRxiv - Genomics 2019Quote: ... About 5×10^5 to 5×10^6 cells were crosslinked with final 1% formaldehyde (Sigma, F8775-500ML) for 10 min ...
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bioRxiv - Cancer Biology 2021Quote: ... We also used primary mouse monoclonal antibodies against anti-α-acetylated tubulin (clone 6-11B-1, Sigma Aldrich) for detecting mitotic spindles as described in Maiorova and Odintsova ...
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bioRxiv - Molecular Biology 2022Quote: ... the nuclear pellet was resuspended in buffer C (40 mM HEPES pH 7.6, 4 mM MgCl2, 0.6% Triton X-100, 0.5% IGEPAL CA-630, 20% Glycerol, 1 mM DTT, Sigma cOmplete EDTA-free Protease Inhibitor Cocktail ...
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bioRxiv - Neuroscience 2023Quote: ... Mouse anti-acetylated tubulin (clone 6-11B-1) was Cat# T6793 (RRID:AB_477585) from Sigma-Aldrich (St. Louis, MO). Rabbit anti-GPSM2 Cat# HPA007327 (RRID ...
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bioRxiv - Molecular Biology 2023Quote: ... Cells were harvested 6 hours post transfection through the addition of 1 mL TRI reagent (Sigma-Aldrich, Merck) directly to the monolayer ...
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bioRxiv - Cell Biology 2023Quote: ... Primary antibodies used were acetylated α−tubulin (1:500, clone 6-11-B1, Sigma-Aldrich, St. Louis, MO), rabbit polyclonal DNALI1 (1:100 ...
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bioRxiv - Biochemistry 2024Quote: ... and after cool down alkylated with 6 μL of freshly prepared 1 M iodoacetamide (IAA, Sigma, #I1149-5G). The alkylation was performed for 15 min at room temperature in darkness ...
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bioRxiv - Physiology 2024Quote: ... and then moved into 6-well-plates containing digestion medium (DMEM + 10% FBS + 1% amphotericin B (A2942, Sigma)) and 10% liberase (Roche ...
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bioRxiv - Cancer Biology 2020Quote: ... the target cells were trypsinized and replated in 6-cm dishes in media containing 2 μg/mL Puromycin (P8833, Sigma-Aldrich Quimica S.A., Madrid, Spain). Selection media was changed every 48-72 h for a minimum of 1 week before cell lines were frozen in 50% FBS and 10% DMSO and stored in liquid nitrogen for later use.
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bioRxiv - Neuroscience 2022Quote: ... To assess either apoptotic and necrotic cell death pycnotic nuclei were identified with 4’,6-diamidino-2-phenylindole (DAPI) staining (Cat# D9542-5MG, Sigma-Aldrich, St Louis, MO, USA).
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Kinetochore individualization in meiosis I is required for centromeric cohesin removal in meiosis IIbioRxiv - Cell Biology 2020Quote: ... Metaphase II oocytes were placed into home-made M16 medium without CaCl2 for 30min to 1h prior to being placed into the activation medium (M16 medium without CaCl2 supplied with 100mM Strontium chloride, Sigma-Aldrich 204463), for 1hour (except for protein detection during early anaphase II in Fig ...
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bioRxiv - Genetics 2019Quote: ... Expression of Scc1(R180D,268D)-HA was induced 1h after starting alpha-factor treatment by addition of newly-made galactose (Sigma-Aldrich, G0750) (2% final ...
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bioRxiv - Genetics 2022Quote: ... Incubation with appropriate secondary HRP-labelled antibody (less than 1h) was followed by detection with Immobilon western chemiluminescent HRP substrate (Merck Millipore, Darmstadt, Germany).
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bioRxiv - Plant Biology 2021Quote: Live-cell imaging of 5-days old Arabidopsis seedlings was performed after 1h treatment with 50 μM BFA (Sigma catalogue no: B7651) and 2 μM FM4-64 (SynaptoRed C2 ...
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bioRxiv - Evolutionary Biology 2020Quote: Whole animals (~100 hatchlings) were dissociated by incubating for 1h at 25°C in a collagenase solution (1mg/mL, Sigma-Aldrich C9722) with continuous agitation ...
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bioRxiv - Biochemistry 2020Quote: ... The DMSO control was then treated with light iodoacetamide while the compound treated sample was incubated with heavy iodoacetamide for 1h each at room temperature (200 μM final concentration, Sigma-Aldrich, 721328). The samples were precipitated by addition of 12.5 µl of 100% (w/v ...
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bioRxiv - Immunology 2023Quote: ... was added for 1h at RT followed by incubation with OPD (o-phenylenediamine dihydrochloride) revelation substrate for 10 min (Sigma-Aldrich, #P8287). Absorbances were analyzed at 492 vs 620 nm on an ELISA plate reader (Berthold).
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bioRxiv - Neuroscience 2023Quote: ... The fixed thin gel cultures were permeabilized in PBST (0.2% (vol/vol) TritonX-100 in PBS) at room temperature for 1h and blocked with blocking buffer (5% (wt/vol) BSA (Sigma, cat. # A2153), 2% (wt/vol ...
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bioRxiv - Cell Biology 2023Quote: ... Fixation was followed by blocking (1h, RT) with 3% bovine serum albumin (BSA/0.01% Tween™20/PBS) (Sigma, Cat#A-7906). Samples were incubated with primary antibodies (overnight ...
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bioRxiv - Cancer Biology 2023Quote: ... and again treated with 20μMZ-VAD-fmk freshly added 1h before adding Gal-9 or Staurosporin (Sigma Aldrich, St. Louis, MO, USA). For long-term assays using patient-derived AML cells (5-7 days) ...
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bioRxiv - Cell Biology 2023Quote: ... Enucleated eyes were immersion fixed in 4% PFA diluted in 1x PBS for 1h at RT and incubated in a hydrogel solution: 4% acrylamide (Sigma, Cat# A4058), 2% bis-acrylamide (Alfa Aesar Cat# J63265) ...
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bioRxiv - Neuroscience 2023Quote: ... rats received 40 mg/kg (ip) of entacapone (Combi-Blocks, Switzerland) 1h before the scan and 10 mg/kg (ip) benserazide hydrochloride (Sigma-Aldrich, Switzerland) 30min before the scan ...
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bioRxiv - Biophysics 2024Quote: ... 2D 1H-15N TROSY-HSQC NMR spectra of TRAP samples loaded in Wilmad® 5 mm 541-PP NMR tubes (Sigma-Aldrich) were recorded at 55 °C (328K ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were stained with DAPI before mounting with Mowiol (12 mL of 0.2 M Tris buffer pH8.5, 6 mL distilled water, 6 g glycerol, 2.4 g Mowiol 4-88, Merck Millipore).
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bioRxiv - Neuroscience 2023Quote: ... Coverslips were shortly rinsed in distilled water and mounted using Mowiöl (12 ml of 0.2 M Tris buffer, 6 ml distilled water, 6 g glycerol, 2.4 g Mowiol 4-88, Merck Millipore). Samples were imaged directly or within the next 48h ...
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bioRxiv - Neuroscience 2023Quote: ... 50 mM 6-OHDA exposure were made from a stock solution of 100 mM 6-OHDA (Sigma-Aldrich) in 20 mM L-Ascorbic Acid (AA) ...
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bioRxiv - Biochemistry 2024Quote: ... Fixed cells were stained with DAPI and mounted on Mowiöl (12 ml of 0.2 M Tris buffer, 6 ml distilled water, 6 g glycerol, 2.4 g Mowiol 4-88, Merck Millipore) for imaging.
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bioRxiv - Cell Biology 2021Quote: ... cells were passaged in a 6 well plates and once confluent transduced with LV-EF1α-rtTA3/PGK-Neomycin plus LV-tetO-MyoD/PGK-Puromycin at 1:1 ratio and supplemented with 6 μg/mL of polybrene (Sigma-Aldrich, TR-1003-G). Transduced MEFs were expanded and selected by sequential antibiotics treatment with MEF medium containing either 1 mg/mL of G-418 solution (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2021Quote: ... 132 mM NaCl, 6 mM KCL, 1 mM CaCL2, 1 mM MgSO4, 1.2 mM K2HPO4, 5 mM glucose, Sigma-Aldrich, and 0.4% (w/v) human serum albumin ...
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bioRxiv - Cell Biology 2020Quote: ... they were anaesthetized with E3 solution/0.16 mg/mL tricaine/1% 1-phenyl-2-thiourea (Sigma) and embedded in 0.5-0.8% low melting agarose ...
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bioRxiv - Neuroscience 2021Quote: ... mouse α-tubulin clone B-5-1-2 (1:1000; Sigma Aldrich, San Luis, MO, U.S.). For immunofluorescence primary antibodies were labeled with Alexa-conjugated secondary antibodies Alexa 488 ...
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bioRxiv - Bioengineering 2022Quote: ... primary antibodies were prepared at 1:100 in a 1:4 blocking solution (2% BSA (Sigma), 10% normal goat serum (Life Technologies ...
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bioRxiv - Immunology 2019Quote: ... cells were overlaid with a 1:1 mixture of 2% agarose:2X 199 Media (Sigma-Aldrich) containing 10 μg/mL chymotrypsin ...
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bioRxiv - Microbiology 2020Quote: ... mouse anti-α-tubulin antibody (1:4,000 dilution, clone B5-1-2; Sigma-Aldrich, Taufkirchen, Germany), mouse anti-capsid p24/p27 MAb AG3.0 [92] (1:250 dilution ...
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bioRxiv - Microbiology 2024Quote: ... and 1 μg/ml L-1-tosylamido-2-phenylethyl chloromethyl ketone (TPCK)-treated trypsin (Sigma Aldrich). Cells were incubated for 6 days at 37°C ...
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bioRxiv - Cell Biology 2023Quote: ... embryos were anesthetized with egg water/0.16 mg/ml tricaine/1% 1-phenyl-2-thiourea (Sigma) and embedded in 0.6% low melting agarose ...