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Citations for Millipore Sigma :
2851 - 2900 of 10000+ citations for 6 METHOXY 2 METHYL 1 PHENYLSULFONYL 1H INDOLE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2021Quote: ... free-floating VTA brain sections were incubated 1h at 4°C in a blocking solution of phosphate-buffered saline (PBS) containing 3% Bovine Serum Albumin (BSA, Sigma A4503) and 0.2% Triton X-100 and then incubated overnight at 4°C with a mouse anti-tyrosine hydroxylase antibody (TH ...
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An innovative hematopoietic stem cell gene therapy approach benefits CLN1 disease in the mouse modelbioRxiv - Neuroscience 2022Quote: ... sections were washed trice with PBS and incubated for 1h at RT with Avidin-Biotin complex (Vector) and then with DAB/H2O2 mix (Sigma-Aldrich).
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bioRxiv - Cancer Biology 2020Quote: ... with/without anti-Flag antibody then incubated (1h at RT) with nanogold-coupled secondary antibody and finally fixed with 2.5% glutaraldehyde EM grade (Sigma Aldrich, #16210) for 1 h ...
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bioRxiv - Cancer Biology 2019Quote: ... Samples were analyzed with either a 3h 15 min non-linear gradient or a 1h 23 min linear gradient from 2.4% acetonitrile (ACN, Sigma, 34998-4L), 0.1% FA to 32% ACN ...
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bioRxiv - Cell Biology 2019Quote: ... The horse radish peroxidase-conjugated antibody was then added for 1h at room temperature and the HRP activity was measured by adding o-phenylenediamine dihydrochloride (Sigma–Aldrich). The reaction was stopped by adding 0.6 M HCl followed by absorbance measurement at 492nm using a SpectraMax 190 plate reader (Molecular Devices).
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bioRxiv - Cell Biology 2019Quote: ... treated (5 Gy) fixed (1h post IR) with formaldehyde (3.7% in PBS) and the PLA assays (Duolink PLA technology, Sigma-Aldrich, DUO92014) performed using manufacturer’s protocol with antibody staining performed as in the standard immunofluorescence procedure with antibodies against V5 tag and Treacle or NBS1 and Treacle
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bioRxiv - Molecular Biology 2023Quote: ... Newly synthesized RNA was then labelled by incubating the cells for 1h with 100 µM 4-thiouridine (4-sU, Sigma-Aldrich). RNA extraction was performed with peqGOLD TriFast Reagent (VWR ...
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bioRxiv - Cancer Biology 2023Quote: ... the tissue fragments were washed with ice cold phosphate-buffered saline (PBS) and were subsequently enzymatically digested at 37°C for 1h using 5 mg/mL collagenase II (Sigma-Aldrich), 10 μM Y-27632 (Cayman Chemicals ...
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bioRxiv - Microbiology 2023Quote: ... was added for 1h at RT followed by incubation with OPD substrate revealing reaction for 10 min (Sigma-Aldrich, MO, USA). Absorbances were analyzed at 495 vs 620 nm on an ELISA plate reader (Berthold ...
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bioRxiv - Biochemistry 2023Quote: ... The protein was incubated for 1h at 4 °C in the presence or absence of 20 or 100 µM FTY720 (Sigma-Aldrich) or fumonisin B1 (Sigma-Aldrich) ...
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bioRxiv - Animal Behavior and Cognition 2024Quote: ... 0-7h old females were acclimatised in the cups for 1h and sugar spiked with Rhodamine B (1mg/25ml of sugar; Sigma R6626) was presented for 2.5-3h at dusk ...
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bioRxiv - Neuroscience 2023Quote: ... Serial sections were placed on glass slides (Matsunami, Osaka, Japan) and stained with 4’,6-diamidino-2-phenylindole (DAPI; D9542, Sigma-Aldrich, St. Louis, MO) for 5 min ...
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bioRxiv - Bioengineering 2019Quote: 1 mM 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES)-NaOH buffer pH 7 (Sigma-Aldrich)
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bioRxiv - Microbiology 2020Quote: ... mouse anti-α-tubulin (1:2,000; clone B-5-1-2, Sigma-Aldrich, cat. # T5168) and mouse anti-MIC2 (1:2500 ...
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bioRxiv - Cell Biology 2021Quote: ... 2 µg pSPAX2 and 1 µg pMD2.G with 1 mg/mL polyethyenimine (PEI, Sigma) in serum-free DMEM ...
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bioRxiv - Cell Biology 2023Quote: ... A total of 2 ml of washing solution 1 (WS1: 1X PBS, 1% (BSA, Sigma), 0.1% Triton X ...
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bioRxiv - Neuroscience 2023Quote: ... the fish were anesthetized or euthanized using 1:1000 or 1:500 2-phenoxyethanol (Sigma), respectively.
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bioRxiv - Genetics 2023Quote: ... 1 µg ml–1 natural mouse laminin and 2 µM arabinosylcytosine (Ara-C, Sigma-Aldrich). After 48 h ...
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bioRxiv - Neuroscience 2024Quote: ... tissue supernatant and serum were incubated 1:1 in 2% Trichloroacetic acid (Millipore Sigma: T9159) overnight (4°C) ...
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bioRxiv - Neuroscience 2024Quote: Neuronal processes in octopus arm were labeled with mouse monoclonal antibody 6–11B-1 (1:500 dilution of ascites fluid; Sigma-Aldrich, Cat#: T6793) and mouse monoclonal antibody SMI-31 (1:500 dilution ...
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bioRxiv - Neuroscience 2021Quote: ... We then prepared a solution of 10 mg/mL 6-hydroxydopamine (6-OHDA; Sigma-Aldrich, H116-5MG) and 0.2% ascorbic acid in saline (0.9% NaCL ...
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bioRxiv - Neuroscience 2024Quote: ... We additionally prepared a solution of 10 mg/mL 6-hydroxydopamine (6-OHDA; Sigma-Aldrich, H116-5MG) and 0.2% ascorbic acid in saline (0.9% NaCL ...
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bioRxiv - Biochemistry 2024Quote: ... Coverslips were rinsed in distilled water and mounted using Mowiöl (12 ml of 0.2 M Tris buffer pH 7.5, 6 ml distilled water, 6 g glycerol, 2.4 g Mowiöl 4-88, Merck Millipore). Samples were imaged immediately or within the next 48 h ...
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bioRxiv - Neuroscience 2022Quote: ... Samples were incubated with primary antibodies overnight at 4C (DARPP-32 1:200 Abcam ab40801; FoxP1 1:500 Abcam ab16645; GAD65/67 1:500 Millipore AB1511; MAP-2 1:1000 Millipore MAB3418) and then washed three times with PBS before an overnight 4C incubation in secondary antibody (Alexa-fluor rabbit 555 IgG ...
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bioRxiv - Neuroscience 2022Quote: ... 2 mM DTT, 1 mM EGTA, 2 mM NaF, 1 μM microcystin, 1 mM benzamidine, 1 mM sodium orthovanadate, and Sigma-Aldrich protease and phosphatase inhibitor cocktails) ...
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bioRxiv - Biochemistry 2020Quote: ... 2-2-2 trifluoroethanol (TFE) (Sigma, #T63002), and 200 mM dithiothreitol (DTT ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Both fins of each genotyped larva were dissected and transferred to a drop of Methyl Cellulose 3% (M0387, Sigma-Aldrich) on a slide for documentation using an Olympus SZX16 (Model SZX2-ILLB ...
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bioRxiv - Microbiology 2022Quote: ... embryos were mechanically dechorionated if necessary and anaesthetized in 0.02 % (w/v) buffered 3-aminobenzoic acid methyl ester (pH 7.0) (Tricaine; Sigma-Aldrich, A5040). The bacterial suspension was then injected into the hindbrain ventricle of casper zebrafish larvae at 2 dpf ...
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bioRxiv - Molecular Biology 2022Quote: ... Cells were allowed to grow for 96 h in drug containing medium (methyl methanesulfonate, Acros Organics 156890250, mitomycin C, Sigma M4287 ...
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bioRxiv - Systems Biology 2020Quote: ... and ddH2O and stained for cell volume with 12.5 μg/ml bis(2,2’-bipyridine)-4’-methyl-4-carboxybipyridine-ruthenium-N-succidimyl ester-bis(hexafluorophosphate) (96Ru, 98-102Ru, 104Ru, Sigma Aldrich) in 0.1 M sodium hydrogen carbonate ...
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bioRxiv - Microbiology 2021Quote: ... the samples were dissolved in 50 μL of 20 mg/ml methoxyamine hydrochloride in pyridine for 1.5 h at 33°C followed by derivatization with N-Methyl-N-(trymethylsolyl)trifluoroacetamide (MSTFA, Sigma Aldrich) for 2 h at 35°C.
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bioRxiv - Neuroscience 2020Quote: ... The lesions were made by injecting N-Methyl-D-aspartic acid (10 μg/μL NMDA in 0.9% saline; Sigma, Germany) into the dorsoventral and mediolateral hippocampus at 8 sites bilaterally at a flow rate of 0.15 μL/min (see (Table 1 for coordinates) ...
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bioRxiv - Biophysics 2021Quote: D-glucose and 3-O-methyl-D-glucose powder for in vitro studies were obtained from Sigma-Aldrich (Milan, Italy). Solutions of D-glucose and 3OMG for in vitro studies were prepared in 10 mM phosphate-buffered saline (PBS 1X) ...
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bioRxiv - Plant Biology 2021Quote: ... residual trypsin activity was measured by monitoring the change in absorbance at 247 nm in presence of increasing concentration of recombinant purified Kunitz Trypsin inhibitor (KTI) when incubated with p-toluene-sulfonyl-L-Arg methyl ester (Sigma). To study the inhibitory action of P235 on KTI action ...
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bioRxiv - Plant Biology 2021Quote: ... The sample was then derivatized by adding 60 μL N-methyl-N-(trimethylsilyl)trifluoro acetamide (MSTFA) (Cat# 69479; Sigma-Aldrich) and incubating for 30 min at 37 °C ...
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bioRxiv - Cell Biology 2021Quote: ... cells were stained with 12.5 μg/ml Bis(2,2′-bipyridine)-4′-methyl-4-carboxybipyridine-ruthenium-N-succidimyl ester-bis(hexafluorophos-phate) (96Ru, 98-102Ru, 104Ru, Sigma Aldrich) in 0.1 M sodium hydrogen carbonate (Sigma Aldrich ...
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bioRxiv - Physiology 2021Quote: ... from the “AngII + L-NAME” group (n=8) were given Nω-nitro-L-arginine methyl ester (L-NAME, Sigma-Aldrich) in drinking water (40 mg/kg/d ...
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bioRxiv - Neuroscience 2021Quote: ... Fatty acids were converted to fatty acid methyl esters (FAME) by heating with 14% boron trifluoride (BF3) in methanol (Sigma) and separated on capillary columns in an HP 5890 Series II GLC equipped with a flame ionization detector ...
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bioRxiv - Neuroscience 2021Quote: ... and 5mM of lysosomal damaging compound and LRRK2 activator (24) L-leucyl-L-leucine methyl ester (LLOMe, Sigma Aldrich #L7393) dissolved in DMSO ...
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bioRxiv - Cell Biology 2021Quote: ... Partial depletion of cholesterol was achieved by incubating the cells in 4 mM methyl-β-cyclodextrin (MβCD, Sigma Aldrich: 332615) at 37°C for 30 min or in 60 μg/ml saponin (Nacalai Tesque ...
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bioRxiv - Genetics 2020Quote: Serial ten-fold dilutions of logarithmic yeast cells were spotted on fresh Synthetic Dextrose (SD)-complete (or SD lacking a specific amino acid to preserve the plasmid) plates with or without different concentrations of Methyl methane sulfonate (MMS)(Sigma) and incubated at 30°C for three days ...
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bioRxiv - Cell Biology 2021Quote: ... sphere (1.5 μm – 3 μm)) coated with 8.45 mM poly(ethylene glycol)methyl ether thiol (Mn 800, Cat. No. 729108, Sigma Aldrich) solution for 2 h at room temperature were used ...
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bioRxiv - Cell Biology 2022Quote: ... Inhibitors and drugs (etoposide, trimethoprim, hydroxyurea, methyl methanesulfonate MMS, HIV protease inhibitors, E64, chloroquine and artemisinin) were from Sigma-Aldrich, Santa Cruz Biotechnology ...
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bioRxiv - Neuroscience 2022Quote: ... All larvae were then anaesthetized in 0.02 % (w/v) buffered 3-aminobenzoic acid methyl ester at pH = 7.0 (Tricaine; Sigma-Aldrich, A5040), immediately de-yolked in de-yolking buffer (55 mM NaCl ...
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bioRxiv - Neuroscience 2022Quote: ... Compounds used in these studies are as follows: N-methyl-D-aspartate (NMDA; 1.5ul of 100mM in PBS; Sigma; M3262), recombinant mouse IL1β (200 ng/dose ...
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bioRxiv - Cancer Biology 2024Quote: ... TRACER scaffolds were manufactured by infiltrating cellulose paper strips (0.5 x 12 cm, Miniminit Products) with poly(methyl methacrylate) (PMMA, 120 kDa) dissolved in acetone (Sigma-Aldrich) at 0.175 g/mL with an Allevi Beta 1 3D printer ...
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bioRxiv - Neuroscience 2024Quote: ... the bound protein was visualized using 100 µl of Tetra-methyl-benzidine liquid substrate (TMB slow kinetic form, Sigma-Aldrich) at RT for 20 min ...
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bioRxiv - Genetics 2024Quote: Serial 10-fold dilutions of logarithmic yeast cells were spotted on fresh synthetic dextrose (SD)-complete (or SD lacking a specific amino acid to preserve the plasmid) plates with or without different concentrations of methyl methanesulfonate (MMS) (Sigma) and incubated at 30°C for 3 days ...
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bioRxiv - Neuroscience 2023Quote: ... the bath application of 30 μM 5-HT together with 50 μM N-methyl-DL-aspartic acid (NMA, Sigma-Aldrich) was applied to the isolated brains ...
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bioRxiv - Immunology 2023Quote: ... HPLC grade methyl tert-butyl ether (MTBE), chloroform, ammonium fluoride (NH4F) (ACS reagent, ≥ 98%) and ammonium formate from Sigma-Aldrich or VWR ...