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Citations for Millipore Sigma :
2951 - 3000 of 10000+ citations for Vertical Electrophoresis Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: ... After transduction cells were cultured in with media supplemented with supernatant from B16 cells expressing GM-CSF and 10uM estradiol (Sigma E8875) to generate macrophage progenitor cell lines or in media supplemented with supernatant from B16 cells expressing FLT3L and 10uM estradiol (Sigma E8875 ...
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bioRxiv - Immunology 2020Quote: In the reconstitution experiments the percentage of live GFP+/propidium iodide− cells was determined by flow cytometry by labelling transfected cells with 0.5 μg/ml of propidium iodide (Sigma-Aldrich, #537059).
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bioRxiv - Immunology 2020Quote: Whole-cell extracts from BMDM and microglia per condition were prepared in ice-cold RIPA cell lysis buffer (Millipore, 20-188). Cell lysates were centrifuged at 13,000 rpm for 10 min at 4 °C and equal amounts of protein were resolved with a 4 to 20 % Bis-tris Gel/MOPS running buffer system (Invitrogen ...
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bioRxiv - Immunology 2020Quote: ... High Five™ Cells (BTI-TN-5B1-4) (Trichoplusia ni) were maintained at 28°C in EX-CELL 405 medium (Sigma) supplemented with penicillin and streptomycin.
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bioRxiv - Genetics 2021Quote: ... cells used for immunocytochemistry were seeded at 5-8 x 104 cells per cm2 onto poly-D-lysine (PDL) (Sigma, P7405) and laminin-coated 12 mm glass coverslips in 24-well plates or PDL and laminin-coated clear bottom imaging 96-well plates ...
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bioRxiv - Cancer Biology 2022Quote: ... cells were incubated overnight in mammary epithelial cell media (DMEM/F12 supplemented with 10% fetal bovine serum (Sigma Aldrich, F2442-500ML) 100 U/mL penicillin-streptomycin (Sigma Aldrich ...
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bioRxiv - Immunology 2022Quote: ADCP assay was performed using ExpiCHO cells stably expressing full-length wild-type SARS-CoV-2 S (target cells) and labelled with PKH67 (Sigma Aldrich) as targets ...
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bioRxiv - Cancer Biology 2022Quote: ... The resulting material was filtered twice on 40-μm cell strainers and depleted of erythrocytes using a red blood cell lysis buffer (Sigma, R7757).
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bioRxiv - Cancer Biology 2022Quote: ... 7×103 cells were resuspended in 100μL of appropriate cell-culture media containing 1μg/mL hydrocortisone (Sigma, H0888, lot no. SLBG4963V) and 5μg/mL insulin (Sigma ...
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bioRxiv - Biochemistry 2022Quote: NAD total levels in MT-4 cells and MT-4 cells infected by HIV-1 were assessed using the NAD/NADH Quantification Kit (Sigma-Aldrich) according to the manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2022Quote: ... was used on cell cultures for 2 hours before the fixation of the cells overnight in 10% neutral buffered formalin solution (Sigma, HT501128). EdU was visualized using a Click-iT Plus EdU Cell Proliferation Kit (Thermo Fisher Scientific ...
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bioRxiv - Genomics 2022Quote: ... a single thymidine block was used to first arrest cells in S phase: cells were plated at 4×10^6 cells per 15 cm plate in medium containing 2mM Thymidine (Sigma, T1895), and were grown for 24 hours ...
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bioRxiv - Immunology 2022Quote: ... T cells were cultured with X-VIVO 15 Cell Medium (Cultek, BE02-060Q) supplemented with 5% AB human serum (Sigma, H4522), penicillin-streptomycin (100 mg/mL) ...
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bioRxiv - Microbiology 2022Quote: ... 10 μl of a 100-fold dilution of beads–protein was incubated 2h at 37°C with 100μl 900-fold diluted plasma samples before addition of THP-1 cells (25,000 cells per well; Millipore Sigma, Burlington, MA). After 19h incubation at 37°C ...
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bioRxiv - Neuroscience 2022Quote: ... ≈ 3×106 human embryonic kidney 293T cells (ATCC, #CRL-1573) were plated on 10-cm cell culture dishes in Dulbecco’s Modified Eagle Medium (DMEM) (Sigma, D-7777) supplemented with 10% fetal bovine serum (Gibco ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were plated either on 13 mm glass coverslips (40,000 cells) or three-compartments MFCs (100,000 cells; Figure 1A) coated with 1 mg/ml poly-L-lysine (Sigma-Aldrich, #P2636) in borate buffer (0.15 M ...
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bioRxiv - Microbiology 2023Quote: ... Cells were initially diluted to 0.5×106 cells/mL in 1.2 L EX-CELL Advanced CHO Fed-batch Medium (Sigma, Cat. No. 14366C). Starting from day 3 ...
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bioRxiv - Cancer Biology 2024Quote: ... cells were seeded in 96-well plates (2 × 103 cells/well) and analyzed with the MTT Cell Growth Assay Kit II (Sigma-Aldrich) according to the manufacturer’s instruction ...
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bioRxiv - Molecular Biology 2022Quote: ... cut into small pieces (< 3mm3) and single cell suspensions were generated by mechanical separation (40 μm Corning cell strainer, Sigma-Aldrich). Pellets were washed once on ice-cold PBS/5 mM EDTA/1x GlutaMax/10 μM Y27632 ...
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bioRxiv - Cancer Biology 2022Quote: ... C2C12 cell line differentiation condition was set up by supplementing the C2C12 cells with 10% Horse Serum (Sigma Aldrich, H1138-100ML) rather than FBS for 5 days ...
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bioRxiv - Bioengineering 2024Quote: ... The HUVECs were transduced to be GFP+ and the MDA-MB-231 were stained with red live cell tracker prior to printing (PKH26 Red Fluorescent Cell Linker, Sigma-Aldrich). Immediately following printing ...
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bioRxiv - Cancer Biology 2023Quote: Protein lysates from cell lines were prepared using the CelLytic™ M Cell Lysis Reagent (Sigma-Aldrich, St. Louis, MO, USA), while CelLytic™ MT Mammalian Tissue Lysis Reagent (Sigma-Aldrich ...
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bioRxiv - Cancer Biology 2024Quote: ... cytoplasmic (cytosolic + microsomal fractions (as described in “cell fractionation” of Methods section) cell extracts were digested with Benzonase (Millipore 70746-3) and precleared with protein-sepharose beads for 1 h at room temperature ...
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bioRxiv - Cancer Biology 2024Quote: ... cytoplasmic (cytosolic + microsomal fractions (as described in “cell fractionation” of Methods section) cell extracts were digested with Benzonase (Millipore 70746-3) and DNase I (Thermo Fisher Scientific EN0521 ...
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bioRxiv - Cell Biology 2024Quote: ... 24h post-treatment cells were washed using room temperature PBS 1X and transferred to an Attofluor Cell Chamber (Sigma Aldrich, A7816), 5 μM of DHE was added in HBBS medium (Hanks’ Balanced Salt Solution ...
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bioRxiv - Molecular Biology 2024Quote: ... and MEFs whole cell extracts were prepared by lysing cells in 4xLDS buffer (-EDTA) containing β-mercaptoethanol and 500 U Benzonase (Millipore) at 10,000 cells / µL for 10-15 min at RT ...
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bioRxiv - Neuroscience 2024Quote: ... One day thereafter remaining virus particles were removed by rinsing the cells with PBS and then the cells were detached using trypsin-EDTA (5 min incubation at 37 °C; Sigma-Aldrich) and reseeded in double-coated 384-well multi-well-plates one day after transduction ...
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bioRxiv - Immunology 2024Quote: ... 100,000 to 200,000 T-allo10 cells were cultured in a 96-well plate for 6h in total of 200 uL of the base T cell medium (see Primary Cells section) with 3 ug/mL brefeldin A (Sigma Aldrich), 2 uM monensin (BD ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: Murine glioblastoma cell line (GL261, Cell Bank from Federal University of Rio de Janeiro - UFRJ, Brazil) was cultured in DMEM (Sigma Aldrich) supplemented with 10 % FBS (Cultilab ...
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bioRxiv - Developmental Biology 2023Quote: ... The cells were counted again and 100,000 cells were seeded onto 5 μg/mL Humanplasma Fibronectin (Sigma Aldrich,1 mg/mL) in PBS coated (1 hour at 37℃ ...
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bioRxiv - Neuroscience 2023Quote: ... for co-immunoprecipitation analysis and into 96-well cell culture plates (3×104 cells/well) coated with 0.01% poly-L-lysine (Sigma-Aldrich, #P9404) for ELISA ...
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bioRxiv - Neuroscience 2022Quote: ... coverslips containing the cells were inverted cell-side down onto pre-cooled gelation chambers (using silicon rings (13mm diameter, 120μL volume, Sigma, Cat# GBL664107)) ...
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bioRxiv - Immunology 2023Quote: ... Staining was conducted in PBS / 1 % BSA (CHO cells and huPBMCs) or PBS / 1 % BSA / 1 % normal mouse serum (bovine cells) (Sigma-Aldrich) for 40 min at 4°C ...
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bioRxiv - Biophysics 2023Quote: ... hTERT-RPE-1-mScarlet-H2b + mNeon-CENPA cells were plated on fibronectin coated coverslips at a density of 6000 cells/ cm2 for thymidine (Sigma, T9250) blocked samples and 3000 cells/cm2 for control samples ...
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bioRxiv - Immunology 2022Quote: The murine GBM cancer cell line GL261 was obtained from NCI and the CT-2A cell line was obtained from Millipore Sigma. GL261 and CT-2A cells were cultured in complete (supplemented with 10% FBS ...
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bioRxiv - Physiology 2023Quote: Protein lysates were prepared from primary lung endothelial cells and human umbilical vein endothelial cells using ice cold RIPA buffer (Sigma Aldrich) with proteases and phosphatase inhibitor cocktails (Thermo Fisher) ...
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bioRxiv - Biophysics 2023Quote: ... Cells were trapped at the G1/S boundary by incubating the cells in media containing 1 mM hydroxyurea (Sigma-Aldrich, H8627) for 18 h ...
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bioRxiv - Microbiology 2023Quote: ... Filtered lentiviral supernatant was introduced to MRC5 cells for FLAG-Raf1 experiments or HFF cells for shRNA experiments in the presence of 5 μg/ml Polybrene (Millipore Sigma). Three hours later ...
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bioRxiv - Neuroscience 2023Quote: ... 1×105 cells were plated on a 12-well cell culture multiwell plate containing poly-D lysine-coated coverslips (Sigma-Aldrich). After 24 hours ...
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bioRxiv - Immunology 2023Quote: Five million cells were marked using the PKH-26 Red Fluorescent Cell Linker Kits (Sigma-Aldrich, catalog PKH26GL St. Louis, MO). Following the manufacture instructions ...
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bioRxiv - Microbiology 2023Quote: ... Clonal populations were obtained by limiting dilution and cell culture growth was monitored with an automatic Muse cell analyzer (Merck Millipore, Paris).
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bioRxiv - Systems Biology 2023Quote: The cell toxicity of the drugs was determined using Cell Counting Kit-8 (CCK-8) (Catalog No.: 96992-100TESTS-F, Sigma-Aldrich) according to the manufacturer’s directions ...
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bioRxiv - Immunology 2023Quote: ... These cells can be matured to become robust pro-inflammatory cytokine-producing cells by Phorbol 12-Myristate 13-Acetate (PMA, Sigma-Aldrich). Transduced-THP-1 cells were incubated with PMA (200 ng/mL ...
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bioRxiv - Microbiology 2023Quote: ... at 37°C and 5% CO2. Human BLaER1 cells (a kind gift of Thomas Graf) (Rapino et al. 2013) cells were grown in RPMI (Sigma-Aldrich) supplemented with 10% FCS and 2 mM L-glutamine at 37°C and 5% CO2 ...
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bioRxiv - Cancer Biology 2023Quote: ... at 37°C for 5 min and then seeded onto a Matrigel-coated cell culture dish at 50,000 cell/cm2 in mTeSR1 supplemented with 5 µM ROCK inhibitor Y-27632 (Sigma, Cat#: Y0503) (day-3 ...
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bioRxiv - Immunology 2023Quote: Bone marrow cells were collected from the tibia and femur of B6 mice and treated with red blood cell lysis buffer (Sigma Aldrich). MΦs were generated by incubating bone marrow cells at 37°C with 40 ng/ml M-CSF (Biolegend ...
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bioRxiv - Immunology 2023Quote: ... Human ovarian cancer cell line COV362 obtained from European Collection of Authenticated Cell Cultures (Cat.No. 07071910) were maintained in DMEM (Sigma, Cat.No. D6429) culture medium supplemented with 10% (v/v ...
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bioRxiv - Bioengineering 2023Quote: The effects of MALAT1 siRNA on hMSCs proliferation were characterized with a cell proliferation assay (Cell Counting Kit-8, CCK-8 assay, Sigma Aldrich). hMSCs were seeded in a flat-bottom 96-well tissue culture plate at a concentration of 2000 cells per well with a volume of 100 µL ...
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bioRxiv - Immunology 2023Quote: ... These were subsequently plated in a 96-well V-bottom plate either in T cell media or T cell media containing PMA (50 ng/mL; Sigma-Aldrich), Ionomycin (500ng/mL ...
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bioRxiv - Cancer Biology 2023Quote: ... cells were washed 3 times with 1x PBS and incubated until analysis in cell culture medium supplemented with gentamicin (Sigma, G1272). As positive control ...