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Citations for Millipore Sigma :
2951 - 3000 of 10000+ citations for 6 6 DIMETHYL 4 OXO 4 5 6 7 TETRAHYDRO 1 BENZOFURAN 3 CARBOXYLIC ACID since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2022Quote: ... Cells were plated on 6-well plates pre-coated with poly-L-lysine (10 μg ml-1, Sigma-Aldrich, St. Louis, MO, USA) and cultivated in DMEM medium (PAA Laboratories GmbH ...
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bioRxiv - Molecular Biology 2021Quote: ... male WT (GR fl/fl, Cre/−) and GR knockout (GR fl/fl, Cre/+) mice (N=6 per group) were administered tamoxifen (T5648, Sigma, 5 mg/kg ip in corn oil) once daily for 2 d ...
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bioRxiv - Plant Biology 2023Quote: CFDA: A 100 μl drop of 5(6)-carboxyfluorescein diacetate (CFDA, optimum excitation 490 nm, emission 515 nm) (C8166, Sigma, stock solution 50 mg/ml in acetone) was applied to the cut edge of a single cotyledon at a concentration of 0.1 mg/ml in distilled water ...
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bioRxiv - Neuroscience 2021Quote: ... sections were incubated for 3 days at 4°C with rabbit anti-AVP (1:2,000, Sigma, PC234L) or rabbit anti-Ntng1 (1:1,000 ...
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bioRxiv - Cell Biology 2021Quote: ... cells were passaged at 1/8 ratio every 3 or 4 days with Accumax (Millipore, ref. SCR006).
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bioRxiv - Pathology 2019Quote: ... a cell-permeant NO scavenger 2-(4-car-boxyphenyl)-4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide (cPTIO) (Sigma) at a high concentration of 500 μM was included ...
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bioRxiv - Biochemistry 2019Quote: ... 3 µl 16 mM Tris[(1-benzyl-1H-1,2,3-triazol-4-yl)methyl]amine (TBTA) (Sigma-Aldrich) prepared in 20% DMSO and 80% t-butanol (Sigma) ...
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bioRxiv - Plant Biology 2021Quote: ... The samples were prepared in 1:1 mixture of H2O-ACN with 0.1% TFA by mixing an equal amount of peptide fractions (0.5 μl) with α-cyano-4-hydroxycinnamic acid (CCA)/2,5-dihydroxy benzoic acid (DHB) matrix (Sigma) and spotted on a stainless-steel plate and air-dried ...
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bioRxiv - Immunology 2023Quote: ... 5 ml of PBS and 5 ml of 4% Dextran (Sigma-Aldrich, 31392-50G) in PBS (Gibco ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were then blocked in 1% BSA for 20 minutes before being incubated in a primary antibody against acetylated α tubulin (1:4000 dilution, clone 6-11B-1, Sigma-Aldrich, St. Louis, MO, USA), GFP (1:1000 ...
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bioRxiv - Developmental Biology 2022Quote: ... Antibodies used in this study were: anti-acetylated α-tubulin (Antibody registry ID: AB_477585, clone 6-11B-1, mouse IgG2b, Sigma, diluted 1:250 in blocking solution), anti-SV2 (AB_2315387 ...
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bioRxiv - Bioengineering 2021Quote: ... we PEGylated our microspheres by functionalizing carboxylic acid groups on the microsphere’s surface with 2,000 Da Poly(ethylene)glycol (PEG) diamine molecules (Sigma, 753084) via EDC chemistry ...
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bioRxiv - Cell Biology 2024Quote: ... Auxin-inducible degradation of proteins was induced by treatment with 0.5 mM indole-3-acetic acid (IAA) (Sigma, I2886-5G, CAS: 87-51-4) and 4 µM phytic acid dipotassium salt (Sigma ...
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bioRxiv - Pathology 2024Quote: ... concentrated 10 times using Amicon Ultra-4 Ultracel-3 3 kDa centrifugal filter units (Millipore, UFC800308). Aliquots of concentrated media and flowthrough media were frozen at −80°C.
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bioRxiv - Pathology 2024Quote: ... concentrated 10 times using Amicon Ultra-4 Ultracel-3 3 kDa centrifugal filter units (Millipore, UFC800308). Aliquots of concentrated medium and flowthrough medium were frozen at −80°C.
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bioRxiv - Physiology 2022Quote: ... hatching was induced by placing embryos in chilled (4°C) 1 g/L humic acid solution (Sigma-Aldrich, 53680) and incubating them in that solution overnight at room temperature ...
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bioRxiv - Physiology 2019Quote: ... general Cl− transport was inhibited with 4,4’-diisothiocyano-2,2’-stilbenedisulfonic acid (DIDS, 100 μmol L−1, N = 4) (all chemicals from Sigma Aldrich, St ...
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bioRxiv - Genomics 2024Quote: ... Neurons were observed 4 days after replating aggregates treated with 1 μM retinoic acid (RA, Sigma-Aldrich, reference R2625) for 48 hours ...
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bioRxiv - Neuroscience 2020Quote: ... The signal was determined by nitroblue tetrazolium (NBT) and 5-bromo-4-chloro-3-indolyl phosphate (toluidine salt) (BCIP) (Sigma, 72091).Semithin sections for toluidine blue myelin staining and ultrathin section for TEM imaging were prepared according to our previous protocols (Zhang et al. ...
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bioRxiv - Molecular Biology 2020Quote: ... at 60°C and developed using nitro blue tetrazolium and 5-bromo-4-chloro-3-indolyl phosphate (NBT/BCIP) (Sigma Aldrich) for 60 minutes ...
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bioRxiv - Neuroscience 2019Quote: ... and spinal cords of both sexes (n = 3 each) were fixed in 4% PFA and embedded in 5% agarose (Type IX-A; Sigma-Aldrich) supplemented with 20% sucrose ...
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bioRxiv - Developmental Biology 2020Quote: ... Fertilized oocytes were collected from 3-4 week-old superovulated C57Bl6 females prepared by injecting 5 IU each of pregnant mare serum gonadotrophin (PMSG) (Sigma Aldrich) and human chorionic gonadotropin (hCG ...
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bioRxiv - Microbiology 2020Quote: ... a 1:10,000 anti-rabbit dilution of IgG alkaline phosphatase was used before detection with NBP-BCIP (nitroblue tetrazolium chloride–5-bromo-4-chloro-3′-indolylphosphate; Sigma-Aldrich) as the substrate.
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bioRxiv - Plant Biology 2021Quote: ... immunodetection of the DIG-labelled probes was carried out by using an anti-DIG antibody coupled with alkaline phosphatase and a ready-to-use 5-bromo-4-chloro-3-indolyl phosphate (BCIP)/nitro-blue tetrazolium (NBT) solution (Sigma-Aldrich) as the chromogenic substrate for the enzyme ...
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bioRxiv - Bioengineering 2022Quote: ... Bone-marrow derived hMSCs (hBMSCs) were frozen at passage 3 or 4 with 5*106 cells/ml in freezing medium containing FBS (BCBV7611, Sigma-Aldrich) with 10% DMSO and stored in liquid nitrogen until further use ...
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bioRxiv - Developmental Biology 2020Quote: ... The staining reactions lasted between 2h and 8h at room temperature using nitro-blue tetrazolium (NBT) and 5-bromo-4-chloro-3′-indolyphosphate (BCIP) staining reactions (Sigma Aldrich). Embryos were then rinsed in PBS-T (0.05% Tween-20) ...
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bioRxiv - Plant Biology 2020Quote: ... and placed in Petri dishes filled with 0.5% agarose containing 35 mM β-mercaptoethanol (v/v) and 1.5 mg/mL of 5-bromo-4-chloro-3-indolyl-β-D-galactoside (Sigma). For X-gal staining ...
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bioRxiv - Cell Biology 2019Quote: ... for 1 h at RT and developed in a solution of nitroblue tetrazolium chloride (NBT) and 5-brom-4-chlor-3-indoxylphosphate (BCIP; Sigma-Aldrich) for 5-30 min at RT ...
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bioRxiv - Microbiology 2022Quote: ... Detection was performed by colorimetry using nitro blue tetrazolium chloride / 5-bromo-4-chloro-3-indolyl-phosphate (NBT-BCIP, Sigma-Aldrich) as a substrate.
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bioRxiv - Neuroscience 2024Quote: ... The medium was changed every 3–4 d and enriched with granulocyte-macrophage colony-stimulating factor (5 ng/ml GM-CSF, Sigma-Aldrich). After approximately 14 d at 37°C and 5% CO2 ...
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bioRxiv - Developmental Biology 2024Quote: 4-Hydroxytamoxifen (4-OHT) Treatments and Bromodeoxyuridine Incorporation: 4-OHT (T176, Sigma) treatment was performed as previously described (Mosimann et al. ...
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bioRxiv - Neuroscience 2022Quote: ... Sections were incubated in PBS containing 0.5% normal donkey serum and 0.03% Triton X-100 for 1 h at room temperature (RT, 23 ± 7□) and then stained with primary antibodies overnight at 4□ (rabbit anti-c-Fos, 1:5000, Sigma-Aldrich; rat anti-CTIP2 ...
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bioRxiv - Genetics 2019Quote: ... 1000 μg whole cell lysates were incubated with the indicated anti-Flag M2 affinity gel and monoclonal anti-HA agarose for 3-4 hr at 4 degree (Millipore Sigma). Immunoprecipitants were washed five times with NETN buffer (20 mMTris ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: For multiphoton microscopy kidneys were fixed in 4% paraformaldehyde overnight at 4 °C and embedded in 3% low-melting point agarose (Sigma- Aldrich). Sections (500 µM ...
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bioRxiv - Immunology 2022Quote: ... 10 µl of hemolymph was mixed with 90 µl of 3, 4-Dihydroxy-L-phenylalanine (L-DOPA, 4 mg/ml)(Sigma Aldrich) dissolved in nuclease free water (Cytiva) ...
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bioRxiv - Cancer Biology 2023Quote: Organoids were seeded in 15-well Ibidi chambers after passaging and fixed 3-4 days later in 4% Paraformaldehyde (Sigma-Aldrich) for 60 minutes at room temperature ...
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bioRxiv - Cancer Biology 2023Quote: ... cleared biopsies were blocked for 3–4 h at room temperature (RT) or overnight at 4°C in blocking buffer (PBS (Sigma-Aldrich), 10% Fetal Bovine Serum (FBS ...
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bioRxiv - Plant Biology 2023Quote: ... Supernatants were incubated for 3-4 h at 4 °C with 15-20 μL of ANTI-FLAG® M2 Affinity Gel (Sigma) and washed 3-4 times with Co-IP wash buffer containing 0.1% Triton-100 but without Phosphatase Inhibitor Cocktails and Protease Inhibitor Cocktail ...
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bioRxiv - Immunology 2024Quote: ... The filtered supernatant was then concentrated using a centrifugal filter unit with a 3 kDA cutoff at 4°C (Amicon Ultra-4 Centrifugal filters, Millipore, USA). Concentrated supernatant was stored in 200 µL aliquots at −80°C until further analysis.
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bioRxiv - Immunology 2019Quote: ... 4°C for 5 min and washed once with a PBS containing 1% BSA (Sigma Aldrich). The single cell suspensions used for 10X Genomics scRNAseq analysis were cleared of red blood cells by using a Red blood cell lysis buffer (eBioscience ...
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bioRxiv - Cell Biology 2023Quote: ... zymosan (life technologies, added at a 1:5 ratio) or R848 (Sigma-Aldrich; 4 μg/ml). After this time ...
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bioRxiv - Immunology 2020Quote: ... resuspended in 4°C cryopreservation medium consisting 90% fetal bovine serum (FBS, HYCLONE, Cat. sh30084.03) and 10% Dimethyl sulfoxide (DMSO, Sigma, Cat. D4540) and then placed in Mr ...
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bioRxiv - Cell Biology 2020Quote: ... 25×106 cells were plated on 6-well culture dishes coated with human fibronectin (Sigma, St Louis, MO) and maintained in endothelial cell medium-2 (EGM-2 - Clonetics ...
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bioRxiv - Molecular Biology 2020Quote: ... here the pH of SM or IMF were adjusted to pH4.6 using 6 N HCl (Sigma-Aldrich, Ireland) to remove caseins ...
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bioRxiv - Immunology 2019Quote: ... 6-mm skin biopsy punch samples were digested for 30 minutes at 37°C using Collagenase VIII (Sigma). At the end of the digestion ...
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bioRxiv - Developmental Biology 2019Quote: ... Samples were cut at a 6 μm thickness and stained with Haematoxylin (Electron Miscroscopy Sciences) and Eosin (Sigma). For Alcian blue PAS staining ...
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bioRxiv - Bioengineering 2019Quote: ... or standard 6-well tissue culture plastic dishes were incubated with human plasma fibronectin (Millipore Sigma, Catalog #FC010) at a concentration of 10 μg/ml diluted in PBS (3 ml/well of ECM protein solution added ...
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bioRxiv - Developmental Biology 2020Quote: ... coverslips were incubated 6 min in PBST containing a final concentration of 2 µg/mL DAPI (Sigma-Aldrich) and washed three times for 10 min with PBST ...
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bioRxiv - Cell Biology 2020Quote: ... the remaining mesenchymal fraction was incubated with 6 mg/ml Dispase II/0.05% trypsin solution (Sigma-Aldrich, 04942078001) supplemented with 1 U/ml DNaseI (Sigma ...
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bioRxiv - Cancer Biology 2020Quote: ... 20 ng/ml hyper interleukin-6 (kindly provided by S. Rose-John) and 0.2% Methylcellulose (Sigma-Aldrich, Germany). Cultures were monitored weekly for sphere growth.