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Citations for Millipore Sigma :
251 - 300 of 10000+ citations for Recombinant Mouse PPAR gamma Protein His tag since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2022Quote: ... It was cloned in the expression vector pET30 Ek/lic containing an N-terminal His-tag with the help of the ligation independent Ek/lic system according to the protocol proposed by Novagen, UK ...
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bioRxiv - Immunology 2020Quote: ... The elimination of the N-terminal His tag was performed through the cleavage with biotinylated thrombin included in the Thrombin Cleavage Capture Kit (Merck, Millipore) following manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2022Quote: ... Grids were blocked in 2% BSA in TBST for 30 minutes and then moved into a 20 µl drop of anti-His tag primary antibody (Sigma) diluted 1:100 ...
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bioRxiv - Biochemistry 2023Quote: The NP gene from strain A/WSN/33 (H1N1) with a His-tag at its C-terminal was cloned in the pET22 plasmid (Novagen). Single point mutation R416A was introduced through the Quick-Change kit (Stratagene ...
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bioRxiv - Molecular Biology 2023Quote: Wild type and 2xSE variant of MpRBOHBN with an N-terminal 6 × His tag were expressed in Escherichia coli Rosetta-gami 2(DE3) (Novagen). Cells were grown at 37°C in lysogeny broth (LB ...
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bioRxiv - Biochemistry 2023Quote: Expression of myrArf1 proteins with C-terminal 6x His tags was accomplished via co-expression with yeast N-myristoyltransferase (yNMT) using the pETDuet-1 vector (Novagen) in E ...
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bioRxiv - Molecular Biology 2024Quote: ... Protein purification steps were analyzed by 12.5% SDS-PAGE and WB with the His-Tag Antibody HRP Conjugate Kit (Merck-Millipore, Germany). Protein concentration was determined using the Bradford method (Bio-Rad ...
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bioRxiv - Plant Biology 2024Quote: ... 1035 bp) were cloned with Platinum™ SuperFi™ DNA Polymerase (www.thermofisher.com) into a pCDFDuet His-tag vector (Novagen, www.merckmillipore.com) using two forward primers containing SacI site and a common reverse primer with KpnI site (5′-ATTGAGCTCGATGGCGGCGAGCGAGGGCGT-3′ and 5′-ATTGAGCTCGGCCAGCAGCGGCTACGAAGGGA-3′ ...
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bioRxiv - Cancer Biology 2024Quote: PC4 cDNAs wild type or carrying the W89A mutation and with a N-terminal 6x His-tag sequence were cloned into a pET-Duet1 plasmid (Merck Millipore) and transformed into competent BL21 cells (Invitrogen) ...
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bioRxiv - Genetics 2023Quote: ... Protein expression was verified and quantified by Western blot using recombinant GST protein (Sigma, G5663) as expression standards ...
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bioRxiv - Biophysics 2020Quote: ... the soluble His-tagged proteins were produced and purified after binding to a Ni-NTA His-Bind resin (Sigma-Aldrich, MO, US) as described by Martínez-Martínez (19) ...
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bioRxiv - Cancer Biology 2022Quote: ... horseradish peroxidase (HRP)-conjugated mouse anti-FLAG tag antibody (A8592, Sigma-Aldrich) was used to detect VH binding ...
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bioRxiv - Microbiology 2024Quote: ... HRP conjugated anti-FLAG-tag mouse mAb (M2; Sigma-Aldrich, MO, USA) was used in Figs ...
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bioRxiv - Developmental Biology 2024Quote: ... mouse anti c-myc tag (Clone 9E10 1:100) from Sigma-Aldrich; rabbit anti DsRed (Polyclonal 1 ...
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bioRxiv - Cell Biology 2021Quote: ... P3 2M (AMS biotechnology #GSC-6201G 2M or #GSC-6101G 7M) and LIF ESGRO® Recombinant Mouse LIF Protein (1000 units/mL) (Millipore # ESG1107). For induction of the transgene Stemolecule Doxycycline hyclate 10 mg (Stemgent#04-0016 ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were incubated with primary antibody in blocking solution (1:500 mouse anti-gamma tubulin (Sigma Aldrich, GTU-88), 1:3000 mouse anti-ARL13B (Biolegend ...
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bioRxiv - Genetics 2023Quote: ... and the basal body was stained with mouse anti-gamma tubulin antibody (clone GTU-88 1:5000, Sigma Aldrich). Following overnight incubation at 4°C with primary antibodies diluted in PBS 1X buffer supplemented with 3% BSA and 0.1% Triton X-100 ...
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bioRxiv - Biochemistry 2021Quote: ... Recombinant proteins were induced in Rosetta™ (DE3) Competent Cells (Novagen) with 0.02% (w/v ...
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bioRxiv - Biophysics 2021Quote: ... The recombinant proteins were first purified by Ni2+-affinity column (Novagen) under native conditions and subsequently in-gel cleavage by TEV protease was conducted ...
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bioRxiv - Cell Biology 2020Quote: ... To induce recombinant proteins isopropyl-β-D-thiogalactoside (IPTG, Sigma-Aldrich) was added (0.1 mM ...
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bioRxiv - Cell Biology 2021Quote: Recombinant protein expression vectors were constructed from the pET28a series (Novagen) encoding an N-terminal hexa-histidine tag and thrombin cleavage site.
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bioRxiv - Developmental Biology 2024Quote: ... Recombinant protein was produced in Escherichia coli strain BL21(DE3) (Novagen). The cells were grown in LB medium at 37°C and the protein expression was induced at OD600 of 0.7 with 0.2 mM IPTG (Isopropyl β-d-1-thiogalactopyranoside ...
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bioRxiv - Biophysics 2022Quote: ... The recombinant proteins were first purified by Ni2+-affinity column (Novagen) under native conditions and subsequently in-gel cleavage by TEV protease was conducted ...
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bioRxiv - Plant Biology 2023Quote: ... The recombinant fusion protein was purified with Glutathione Sepharose beads (Sigma). Definite probes were designed for different cis-elements and their mutated versions (Table S3) ...
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bioRxiv - Evolutionary Biology 2024Quote: All recombinant proteins were expressed using pET-11a (Sigma #69436-3) based vectors in BL21(DE3 ...
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bioRxiv - Developmental Biology 2022Quote: ... and Gamma Tubulin 1:200 (Sigma, GTU-88); rat antibody against DE-cadherin 1:20 (DSHB ...
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bioRxiv - Developmental Biology 2021Quote: ... 100 nM gamma-secretase inhibitor XX (EMD Millipore) was added to the expansion medium and was replenished every other day.
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bioRxiv - Cell Biology 2022Quote: ... anti-gamma tubulin (Sigma Aldrich T6557; 1:500); anti-Smo (Santa Cruz sc-166685 ...
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bioRxiv - Neuroscience 2022Quote: ... Rabbit polyclonal gamma tubulin antibody (Sigma, Cat#-T5192). Goat polyclonal ankyrin-G antibody (Paez-Gonzalez et al. ...
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bioRxiv - Cell Biology 2023Quote: ... rabbit polyclonal anti-gamma-tubulin (T3559, Sigma-Aldrich), rabbit polyclonal anti-PRC1 (sc-8356 ...
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bioRxiv - Developmental Biology 2023Quote: ... and Gamma Tubulin 1:200 (Sigma, GTU-88); and chick antibody against GFP 1:1000 (Aves Labs ...
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bioRxiv - Cell Biology 2024Quote: ... anti-gamma-tubulin (Sigma-Aldrich T6557; 1:500). AlexaFluor-labeled secondary antibodies (Thermo Fisher ...
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bioRxiv - Biochemistry 2020Quote: ... N-terminally His-tagged MpPA14 was incubated with the array and binding of the lectin to the polysaccharides was detected by an anti-His tag secondary antibody conjugated to alkaline phosphatase (Sigma-Aldrich). Microarray probing and quantification were performed as previously described(41).
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bioRxiv - Molecular Biology 2021Quote: ... un-spliced precursor and mutant SufB proteins carrying an N-terminal 6X-His-tag were over-expressed in BL21 (DE3) E.coli cells via IPTG (500µM) (Sigma 367-93-1) induction at 37°C for 4hrs ...
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bioRxiv - Biophysics 2020Quote: ... The lysate was nutated with 200 μL of Ni-NTA resin (Roche cOmplete™ His-Tag purification resin, Millipore Sigma #5893682001) at 4°C for 1 hour ...
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bioRxiv - Molecular Biology 2024Quote: pDest17 plasmids containing an N-terminal 6x-His tag and Flag-tagged ARH3 wildtype or H182R were transformed into Rosetta (DE3)pLysS Competent Cells (Sigma, #70956). An initial 5 mL culture was grown overnight in Lennox LB broth supplemented with 100 μg/mL of ampicillin and 25 μg/mL of chloramphenicol at 37 °C while shaking at 200 rpm ...
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bioRxiv - Microbiology 2024Quote: AdfN and AdfN(K43A) were expressed from pET vectors with a 6x-His tag in Rosetta 2 (DE3) cells (Millipore Sigma). For expression ...
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bioRxiv - Biophysics 2024Quote: ... and H3.1 were subcloned into a pET15b-derived plasmid containing an N-terminal 6×His tag (MGSSHHHHHHSSG) in which the thrombin cleavage site of pET15b (Merck Millipore, 69661) was replaced by the TEV protease recognition sequence ...
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bioRxiv - Biochemistry 2020Quote: ... The Gag-His6 protein was loaded onto a HIS-select affinity column (Sigma), washed (20 mM Tris-HCl ...
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bioRxiv - Biophysics 2021Quote: His-tagged human PCNA protein was purchased from Sigma Aldrich (catalogue number SRP5117), at a concentration of 6.6 μM ...
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bioRxiv - Molecular Biology 2022Quote: These His-tagged protein lysates were combined with 1 ml nickel beads (Sigma) and incubated at 4 °C/2 hours/rolling ...
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bioRxiv - Molecular Biology 2024Quote: ... the protein was re-applied to His-Select Cobalt affinity gel (Sigma-Aldrich). The flow through fractions were pooled and concentrated (Vivaspin Turbo 3,000 MWCO ...
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bioRxiv - Immunology 2020Quote: ... and 5 ng/ml of mouse recombinant IL-4 (Sigma-Aldrich) for CSR to IgG1 ...
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bioRxiv - Immunology 2024Quote: ... and 5 ng/ml of mouse recombinant IL-4 (Sigma-Aldrich) (L-I) ...
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bioRxiv - Microbiology 2024Quote: Recombinant untagged Ssp4 and His-GST tagged Ssp6 (prepared as below) were coupled to cyanogen bromide activated agarose (Sigma Aldrich) based on the method described in57 ...
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bioRxiv - Biophysics 2021Quote: ... Protein was expressed as a His6-tag fusion in BL21(DE3) trxB (Novagen) using autoinduction24 ...
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bioRxiv - Physiology 2020Quote: ... or mouse anti-S-tag (for S-tagged NTD, 1:1500, Sigma, SAB2702204) and rabbit anti-Nav1.5 (1:150 ...
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bioRxiv - Cell Biology 2023Quote: ... anti-FLAG-tag (1:5,000, mouse mAb, F1804, Sigma-Aldrich, St. Louis, MO), anti-alpha-tubulin (1:2,000 ...
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bioRxiv - Microbiology 2023Quote: Antibodies were as follows: commercial mouse monoclonal antibodies to Flag-tag (M2; Sigma), Strep-tag (4F1 ...
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bioRxiv - Plant Biology 2021Quote: ... were inserted between the NdeI and XhoI sites of the bacterial expression vector pET-24a containing a HIS-tag (Novagen, Madison, USA). The ligated products were transformed into TOP10 E ...