Labshake search
Citations for Millipore Sigma :
151 - 200 of 10000+ citations for Recombinant Mouse PPAR gamma Protein His tag since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Biochemistry 2020Quote: ... The Trx-His(6) tag was cleaved by addition of 300 units of thrombin (Sigma T4648) directly to the eluate and the solution dialyzed (Slide-A-Lyzer dialysis cassettes ...
-
bioRxiv - Biophysics 2020Quote: ... MBP and the His-tag were cleaved from the P domain using HRV 3C protease (Novagen). Cleaved P domain protein eluted from Ni-NTA resin and was further purified by size-exclusion chromatography using a Superdex 16/600 200 pg column (GE Healthcare ...
-
bioRxiv - Plant Biology 2021Quote: ... and GST were fused with a C-terminal octa-His tag in the pET42 vector (Novagen).
-
bioRxiv - Biochemistry 2023Quote: ... The lysate was clarified by centrifugation and applied to cOmplete His-tag purification resin (Sigma Aldrich) for 1 hour at 4 °C with rotation ...
-
bioRxiv - Cell Biology 2022Quote: ... mouse α-His-HRP (A7058, Sigma-Aldrich, 1:10000).
-
bioRxiv - Neuroscience 2022Quote: ... The Flag-tag protein affinity sequence (DYKDDDDK; Sigma-Aldrich) was added to the N-terminal to facilitate confirmation of expression ...
-
bioRxiv - Cancer Biology 2023Quote: ... gamma-secretase inhibitor DAPT (Sigma) at 10µM concentration and recombinant WNT5B protein (7347 ...
-
bioRxiv - Genomics 2023Quote: ... HP1 gamma (Millipore 05-690).
-
bioRxiv - Cell Biology 2020Quote: ... His-tagged proteins were immobilized on Ni-NTA His-Bind® Superflow™ Resin (Merck Millipore, 70691), washed and eluted in SB buffer (30 mM HEPES-NaOH pH 7.5 ...
-
bioRxiv - Microbiology 2020Quote: ... HRP-conjugated mouse anti-FLAG tag Ab (Sigma-Aldrich) was used to detect Fab/VH binding ...
-
bioRxiv - Microbiology 2022Quote: ... mouse monoclonal Flag-tag antibody (Sigma, F3165, 1:1000), rabbit monoclonal HA-tag antibody (CST ...
-
bioRxiv - Microbiology 2020Quote: ... mouse anti-Flag tag (Sigma cat# F1804, 1:1000), rabbit anti-human actin (Sigma cat#A2066 ...
-
bioRxiv - Microbiology 2024Quote: ... FLAG-tag mouse mAb was purchased from Sigma (USA); goat anti-rabbit IgG H&L/HRP was purchased from Bioss (China) ...
-
bioRxiv - Biochemistry 2024Quote: ... 1:1000 mouse anti-FLAG-tag (Sigma Aldrich, F1804), or rabbit anti-CEP290 (Novus ...
-
bioRxiv - Cancer Biology 2024Quote: ... mouse anti-Flag tag (Millipore Sigma, F3165, 1:5000). Membranes were scanned on a LI-COR Odyssey Imager ...
-
bioRxiv - Cell Biology 2024Quote: ... Mouse anti–S-tag was from Novagen (Gibbstown, NJ). Rabbit anti-GFP was from Santa Cruz Biotech ...
-
bioRxiv - Bioengineering 2024Quote: ... mouse anti-FLAG-M2-tag antibody (Sigma–Aldrich, F3165) diluted 1:2000 in 5% milk in PBST or mouse OXPHOS cocktail (abcam ...
-
bioRxiv - Immunology 2020Quote: ... Recombinant SLAMF7 protein (r-SLAMF7) was from Sigma. Purified anti-SLAMF7 (162.1 ...
-
bioRxiv - Cell Biology 2023Quote: ... recombinant IGF-I protein at 10nM (Sigma, #I3769), recombinant IGF-II protein at 10nM (R&D ...
-
bioRxiv - Biophysics 2021Quote: ... followed by cleavage of the His-tag with 3C protease and dephosphorylation with bovine alkaline phosphatase (Sigma). Further purification was performed by ion exchange chromatography (Source Q for WT and the S897E ...
-
bioRxiv - Biochemistry 2021Quote: ... pneumoniae LicB with an N-terminal 10×His affinity tag in a modified pET-19b vector (Novagen) was overexpressed in E ...
-
bioRxiv - Developmental Biology 2024Quote: ... lysed and then incubated with RIP buffer containing magnetic beads conjugated with anti-His tag antibody (Millipore) or corresponding negative control IgG (Millipore) ...
-
bioRxiv - Microbiology 2020Quote: ... Mouse monoclonal HA-Tag and Mouse Monoclonal Flag antibodies purchased from Sigma (USA); Goat anti-mouse IgG horseradish peroxidase-conjugated secondary antibody and goat anti-rabbit IgG horseradish peroxidase-conjugated secondary antibody purchased from Thermo Fisher Scientific (USA).
-
bioRxiv - Microbiology 2021Quote: ... 50 ng/ml mouse recombinant EGF (Sigma-Aldrich), 1X B27 supplement (Gibco) ...
-
bioRxiv - Cell Biology 2022Quote: ... recombinant mouse EGF (0.025μg/ml, Sigma-Aldrich, #SRP3196) and bovine pituitary extract (30μg/ml ...
-
bioRxiv - Cell Biology 2024Quote: ... 50ng/ml mouse recombinant EGF (#SRP3196, Sigma-Aldrich), 1xB27 supplement (#11530536 ...
-
bioRxiv - Cell Biology 2024Quote: ... 103 units/mL recombinant mouse LIF (Sigma-Aldrich) in high-glucose DMEM supplemented with L-Gln ...
-
bioRxiv - Immunology 2021Quote: ... the purified MECU protein was separated on 12% SDS-PAGE and further probed with mouse polyclonal anti-His (Sigma, USA) and rabbit polyclonal anti-H ...
-
bioRxiv - Biochemistry 2024Quote: ... Recombinant human KU70 was detected via an N-terminal FLAG-tag with antibody M2 (Sigma F1804). Recombinant human LIG4 ...
-
bioRxiv - Microbiology 2021Quote: ... Dialysis was performed in concert with cleavage of the His-SUMO tag using the SUMO protease (Sigma Aldrich) at 20U/1mg of protein ...
-
bioRxiv - Biochemistry 2020Quote: ... 2020) genes fused with an N-terminal His-tag sequence have been inserted into a pET28a vector (Novagen) for protein expression in E ...
-
bioRxiv - Biochemistry 2023Quote: ... The supernatant was flown through 500 μL of Roche cOmplete™ His-Tag purification resin (Millipore Sigma, #5893682001) for His-tag tagged proteins ...
-
bioRxiv - Cancer Biology 2021Quote: ... PI3K gamma (Millipore #14-558-K), kinase concentration 0.4 nM ...
-
bioRxiv - Cell Biology 2020Quote: ... anti-gamma-tubulin (T6557, Sigma-Aldrich), and anti-actin (I-19 ...
-
bioRxiv - Bioengineering 2022Quote: ... When DAPT (gamma-secretase inhibitor, Sigma) and BB-94 (ADAM metalloprotease inhibitor ...
-
bioRxiv - Cell Biology 2020Quote: ... gamma-tubulin (Sigma T6557, 1:1000), beta-actin (Santa Cruz sc-47778-HRP ...
-
bioRxiv - Microbiology 2021Quote: ... Interferon-gamma (Gibco™, Merck Millipore), L-tryptophan (Sigma-Aldrich ...
-
bioRxiv - Neuroscience 2023Quote: ... gamma-aminobutyric acid (GABA, A2052, Millipore), Reelin (ab312310 ...
-
bioRxiv - Cell Biology 2023Quote: ... gamma-tubulin rabbit antibody (Sigma Aldrich), Pericentrin rabbit anibody (Abcam) ...
-
bioRxiv - Molecular Biology 2020Quote: ... mouse anti-FLAG tag (clone M2-F1804, Sigma, 1:1000), mouse anti-GFP (clones 7.1 and 13.1 ...
-
bioRxiv - Neuroscience 2024Quote: ... mouse anti-HA-Tag (1:500, rabbit Sigma-Aldrich H3663), anti-FLAG (1:50 ...
-
bioRxiv - Cell Biology 2024Quote: ... mouse monoclonal anti-myc tag [4A6] (EMD Millipore, 05-724), mouse monoclonal anti-M6PR (cation independent ...
-
bioRxiv - Cell Biology 2024Quote: ... mouse monoclonal anti-myc tag [4A6] (EMD Millipore, 05-724), goat polyclonal anti-VPS35 (Abcam ...
-
bioRxiv - Bioengineering 2022Quote: ... Plates were coated with either recombinant GFP protein (Millipore) at 1 μg/mL to measure GFP-specific responses ...
-
bioRxiv - Plant Biology 2021Quote: ... which yields recombinant proteins fused to hexahistidine tail (Novagen). The correct cloning was confirmed by sequencing.
-
bioRxiv - Immunology 2024Quote: ... 20 μg of Ovalbumin recombinant protein (Sigma-Aldrich, A5503) (1:1 mixed with Alhydrogel ...
-
bioRxiv - Plant Biology 2024Quote: ... His- and GST-tagged proteins were detected with anti-His (Monoclonal Anti-polyHistidine antibody, Sigma H1029, 1:5000 dilution) or anti-GST (Mouse anti-GST monoclonal antibody ...
-
bioRxiv - Cell Biology 2023Quote: ... recombinant mouse EGF (0·025 µg/ml, Sigma-Aldrich), and bovine pituitary extract (30 µg/ml ...
-
bioRxiv - Biophysics 2021Quote: ... the proteins were purified using His-Select Nickel agarose resin (Sigma) and anion exchange chromatography (Source Q ...
-
bioRxiv - Plant Biology 2021Quote: ... Fusion proteins were purified using Ni2+-containing His-Bind columns (Novagen). Purified proteins were desalted and concentrated by ultracentrifugation (Amicon ...