Labshake search
Citations for Millipore Sigma :
251 - 300 of 10000+ citations for Protein Dialysis kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cancer Biology 2024Quote: PLA was performed to detect protein-protein interactions in MDA-MB-231 cells using Duolink™ In Situ Red Starter Kit (Sigma, cat. DUO92101). All the reagents ...
-
bioRxiv - Cell Biology 2020Quote: ... and nucleoproteins were extracted using a nuclear protein extraction kit (Merck Millipore, Darmstadt, Germany). Nucleoproteins were separated by electrophoresis on 6/12% sodium dodecyl sulfate (SDS ...
-
bioRxiv - Physiology 2022Quote: ... Total protein concentration in the samples was determined with a standard BSA kit (Millipore). 15 µL of the sample was diluted with 60 µL ultrapure water in order to keep the concentration within the linear range of the calibration curve (0.2 to 2 µg⋅µL−1) ...
-
bioRxiv - Systems Biology 2020Quote: ... Total protein concentration was measured using bicinchoninic acid assay (BCA Kit, Sigma-Aldrich, USA).
-
bioRxiv - Cell Biology 2021Quote: ... was conjugated with Atto-647N using a protein labeling kit (cat # 76508 Sigma-Aldrich). PDMS stamps were incubated with solution containing 50μg/ml fibronectin and 1μg/ml of the conjugated fibronectin in Dulbecco’s phosphate-buffered saline (Sigma-Aldrich ...
-
bioRxiv - Cancer Biology 2020Quote: RIP assays employed the Magna RIP™ RNA-Binding Protein Immunoprecipitation Kit (Millipore Sigma) following manufacturer’s instructions ...
-
bioRxiv - Cell Biology 2022Quote: RIP assay was done with Magna RIP RNA-Binding Protein Immunoprecipitation Kit (Millipore, USA). Quantitative RT-PCR was used for determining total RNA levels.
-
bioRxiv - Neuroscience 2021Quote: ... Protein concentration was estimated with the Quantipro BCA assay kit (Sigma-Alrich, United States), and lysates were resolved on a 10% sodium dodecyl sulfate polyacrylamide gel prior to transfer onto polyvinylidene fluoride membranes ...
-
bioRxiv - Cancer Biology 2020Quote: ... and membrane fractions were isolated using a Compartment Protein Extraction Kit (Millipore, Billerica, MA) following the manufacturer’s instructions ...
-
bioRxiv - Physiology 2021Quote: ... Soluble protein was measured using a Bicinchronicinc acid kit (BCA1, Sigma-Aldrich Canada Co.). Absorbance of each reaction was measured in a spectrophotometer (Spectra Max M2 ...
-
bioRxiv - Molecular Biology 2022Quote: ... Proteins were detected with an enhanced chemiluminescence assay kit (ECL Plus, Millipore Corporation, USA). Signals were visualized using ImageLab 1.46 (BioRad ...
-
bioRxiv - Pharmacology and Toxicology 2022Quote: ... Protein concentration was determined using the bicinchoninic acid assay kit (Sigma-Aldrich, Pool, UK) using bovine serum albumin as standard ...
-
bioRxiv - Molecular Biology 2022Quote: ... Total or nuclear protein was extracted using a nuclei isolation kit (NUC201, Sigma-Aldrich). Protein samples were electrophoresed on a 10% sodium dodecyl sulfate-polyacrylamide gel and transferred to polyvinylidene fluoride (PVDF ...
-
bioRxiv - Microbiology 2023Quote: ... Protein precipitates were solubilized in 6M urea and subsequently quantified using BCA kit (Sigma). Ten micrograms of the protein samples were reduced with 10mM dithiothreitol (DTT ...
-
bioRxiv - Cancer Biology 2023Quote: ... secondary antibodies were added and the proteins were detected using an ECL kit (Millipore). Immunoreactive signals were visualized using the GE Amersham Imager 600 chemiluminescence system.
-
bioRxiv - Neuroscience 2024Quote: ... Protein concentrations were determined using the Pierce™ BCA assay kit (Sigma-Aldrich, Germany).
-
bioRxiv - Genomics 2022Quote: ... ratio by a Gibson approach with 3h of incubation followed by dialysis for 45 min on a membrane filter (MF-Millipore 0,025 um membrane, Merck). The product was transformed into 10-beta Electrocompetent E.coli (NEB) ...
-
bioRxiv - Bioengineering 2020Quote: ... the NLS peptides were twice dialyzed (first against 10 mM HCl solution for 6 hours and then against ddH2O water for 12 hours) in dialysis tubing with a 2 kDa MW cut-off (Sigma-Aldrich, St. Louis, MO/USA). After dialysis ...
-
bioRxiv - Cell Biology 2020Quote: ... 4-dinitrophenylhydrazine using protein carbonyl content assay kit according to the manufacturer’s instructions (Sigma, USA). The optical density of the adduct was examined at 375 nm ...
-
Inositol depletion regulates phospholipid metabolism and activates stress signaling in HEK293T cellsbioRxiv - Cell Biology 2022Quote: ... Total protein staining of membrane was done using an R-PROB staining kit from Sigma. Primary antibodies were incubated overnight at 4°C and secondaries for 1 hr at RT ...
-
bioRxiv - Molecular Biology 2020Quote: ... The immunoprecipitates were purified using Protein G Immunoprecipitation Kit (Cat. No. IP50, Sigma-Aldrich Inc.) and were separated on SDS-PAGE for western blotting using monoclonal antibodies against T18 as described above ...
-
bioRxiv - Microbiology 2020Quote: The DRM and DSM fractions119 were separated using the CelLytic MEM Protein Extraction Kit (Sigma). 600 μl of lysis and separation buffer were added to 50 μl of resuspended membrane and incubated at 4°C overnight ...
-
bioRxiv - Immunology 2022Quote: ... The protein bands on the PVDF membranes were observed using an enhanced chemiluminescence kit (Millipore) and quantified using Image J software (NIH ...
-
bioRxiv - Physiology 2021Quote: ... The remaining supernatant was precipitated using the Calbiochem Protein Extraction Kit (EMD Millipore, Billerica, MA) according to manufacturer’s instructions ...
-
bioRxiv - Cancer Biology 2022Quote: Tissue proteins were extracted according to kit instructions (Sigma Aldrich, R0278, St. Louis, Missouri, USA) and protein concentrations were routinely quantified by using a BCA kit (Beyotime ...
-
bioRxiv - Cancer Biology 2020Quote: ... Firefly luciferase activity was normalized to protein content using Bicinchoninic Acid Kit from Sigma-Aldrich® and measured with using a luminometer (Centro XS3 LB960 ...
-
bioRxiv - Cell Biology 2021Quote: RIP assays were performed using the Magna RIP RNA-Binding Protein Immunoprecipitation Kit (Millipore, USA) with the mouse anti-Ago2 antibody (Millipore ...
-
bioRxiv - Microbiology 2021Quote: ... coli membrane fractions were determined using a bicinchoninic acid (65) protein assay kit (Sigma-Aldrich) with BSA (Interchim ...
-
bioRxiv - Cancer Biology 2022Quote: RIP was performed according to the Magna-RIPTM RNA-binding protein immunoprecipitation kit (Millipore/Merck) as previously described (Papoutsoglou et al ...
-
bioRxiv - Molecular Biology 2022Quote: RNA immunoprecipitation was performed using the Magna RIP RNA-Binding Protein Immunoprecipitation Kit (Millipore, Billerica) following the manufacturer’s protocol ...
-
bioRxiv - Molecular Biology 2023Quote: ... Protein concentration in the extracts was measured using the QuantiPro BSA Assay Kit (Sigma-Aldrich).
-
bioRxiv - Cancer Biology 2023Quote: RIP experiment was optimized using the Magna RIP RNA-binding protein immunoprecipitation kit (Millipore, USA) in light of the manufacturer’s instructions ...
-
bioRxiv - Microbiology 2023Quote: ... The total protein (μg/mL/OD600) was determined using a Lowry kit (Sigma-Aldrich, TP0300), and bovine serum albumin (BSA ...
-
bioRxiv - Immunology 2024Quote: ... and RIP assays were carried out with Magna RIP RNA-Binding Protein Immunoprecipitation Kit (Millipore) and anti-Flag antibody (Abcam ...
-
bioRxiv - Immunology 2020Quote: ... After dialysis external solutions were mixed and were concentrated by Centricon YM-3 (from Merck-Millipore, used at 5000 x g for 30 minutes, 4 °C) to reach 1 ml as final volume ...
-
bioRxiv - Neuroscience 2019Quote: ... Subsequent labeling of oxidative protein carbonyls was performed using an OxyIHC kit (EMD-Millipore, Cat#: S7450). Derivatization of protein carbonyl groups and all subsequent steps were performed in accordance with the manufacturers instructions ...
-
bioRxiv - Genetics 2020Quote: ... and diluted to measure protein concentration (1:10 diluted samples, Bradford Assay Kit, Sigma-Aldrich, B6916) and ATP level (1:2,000 diluted samples ...
-
bioRxiv - Neuroscience 2022Quote: RNA immunoprecipitation was performed using the Magna RIP® RNA-Binding Protein Immunoprecipitation Kit (Sigma-Aldrich) according to the manufacturer’s protocol ...
-
bioRxiv - Biochemistry 2022Quote: ... Cell lysis and membrane protein extraction was performed using the ProteoExtract Native Membrane kit (Merck Millipore) according to the manufacturer’s protocol ...
-
bioRxiv - Biochemistry 2020Quote: ... proteins were harvested from the cells by subcellular fractionation using ProteoExtract subcellular fractionation kit (Millipore Sigma). Fractionated samples were analyzed by SDS-PAGE and electrophoresis for detection of HA-tagged Nedd4 (Roche anti-HA high affinity,1:2000 dilution ...
-
bioRxiv - Neuroscience 2020Quote: ... Following the estimation of protein concentration using the Quantipro BCA assay kit (Sigma-Alrich, United States), equal amounts of lysate were resolved on a 10% sodium dodecyl sulfate polyacrylamide gel and then transferred onto polyvinylidene fluoride membranes ...
-
bioRxiv - Cancer Biology 2020Quote: RNA-binding protein immunoprecipitation was run according to supplier recommendations (Millipore, Magna RIP Kit, 17-700). Beads were conjugated with 10 μg either anti-IMP3 (#1 ...
-
bioRxiv - Biochemistry 2020Quote: ... Protein concentration of the membrane preparation was determined by the bicinchoninic acid assay kit (Sigma-Aldrich), the sample concentration was adjusted to of 3–10 mg/mL membranes were aliquoted (50 or100 μl) ...
-
bioRxiv - Molecular Biology 2019Quote: RIP experiments were performed using a Magna RIP™ RNA-Binding Protein Immunoprecipitation Kit (Millipore, USA) according to the manufacturer’s instructions ...
-
bioRxiv - Developmental Biology 2020Quote: RNA immunoprecipitation was performed using the Magna RIP RNA-Binding Protein Immunoprecipitation Kit (17-700; Millipore) according to the manufacturer’s instructions ...
-
bioRxiv - Microbiology 2021Quote: RNA-binding protein immunoprecipitation (RIP) was performed using a Magna RIP kit (Cat# 17-700, Millipore) according to the manufacturer’s protocol ...
-
bioRxiv - Molecular Biology 2022Quote: ... we performed a RIP assay using Magna RIP RNA-Binding Protein Immunoprecipitation Kit (Millipore, Burlington, MA). HDFa were lysed in RIP lysis buffer ...
-
bioRxiv - Microbiology 2022Quote: ... The protein concentrations of the samples were determined by the BCA assay kit (Sigma-Aldrich, USA). Digitonin (Calbiochem ...
-
bioRxiv - Plant Biology 2023Quote: Nuclear proteins were extracted from frozen poplar stem using the CellLytic™ PN kit (Sigma, USA) according to the manufacturer’s instruction ...
-
bioRxiv - Cell Biology 2022Quote: Detection of an interaction between proteins was assessed using the Duolink II Detection Kit (Sigma-Aldrich) according to the manufacturer specifications ...