Labshake search
Citations for Millipore Sigma :
151 - 200 of 10000+ citations for Protein Dialysis kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Biochemistry 2023Quote: ... and an Atto 647N protein labeling kit (Sigma-Aldrich, 76508) were used ...
-
bioRxiv - Neuroscience 2024Quote: ... 2015 using a compartment protein extraction kit (Millipore cat: 2145). Triturated brain tissue was thawed on ice for ∼30 minutes before being homogenized in 2 ml/g of Buffer C (HEPES (pH7.9) ...
-
bioRxiv - Evolutionary Biology 2020Quote: ... at 4°C in dialysis buffer (100 mM NaCl, 20mM Tris pH 7.4, containing 15 μl bovine thrombin (605157, Merck Millipore) to cleave the His-tags) ...
-
bioRxiv - Molecular Biology 2021Quote: ... which was carried out as two rounds of dialysis against 1 mM DTT in H2O and one round against 0.5 mM TCEP (Sigma) before lyophilisation and storage ...
-
bioRxiv - Microbiology 2021Quote: ... Reaction products were routinely desalted using “lily pad dialysis” (the entire above reaction volume placed on a slowly rotating 0.05 um HA filter (Millipore) floating on the surface of a MilliQ water ...
-
bioRxiv - Cell Biology 2021Quote: ... 1000 units of SUMO Protease 1 (EZCut) was added in a Pur-A-Lyze dialysis tube (Sigma, 3.5 MWCO), which was incubated at 4°C overnight in 1 L of SUMO cleavage buffer (50 mM Tris HCl pH 8 ...
-
bioRxiv - Microbiology 2021Quote: ... we prepared and placed coral lysates into dialysis tubing (D9777, 14 kDa for molecular weight cutoff; Sigma, Aldrich, USA) and co-incubated the dialysis tubing in the bacterial culture at a specific temperature ...
-
bioRxiv - Developmental Biology 2023Quote: ... DNA was purified by the drop dialysis method using the MF-Millipore Membrane Filter (Merck Millipore, Cat. No. #VCWP04700). Length distribution of the genomic DNA was analyzed by pulsed-field gel electrophoresis ...
-
bioRxiv - Microbiology 2022Quote: ... Liposomes were then separated from an excess of free calcein by 48 h - dialysis (Float-A-Lyzers, Sigma-Aldrich) against PBS ...
-
bioRxiv - Plant Biology 2020Quote: ... The protein amount of the resuspended membrane fractions was quantified by Bicinchoninic Acid Protein Assay Kit (Sigma-Aldrich) and equilibrated between different samples ...
-
bioRxiv - Cell Biology 2021Quote: ... Protein concentration was measured using Pierce™ BCA Protein Assay Kit (Sigma-Aldrich, St. Louis, Missouri United States) following the manufacturer’s instructions ...
-
bioRxiv - Neuroscience 2020Quote: ... solium larval extracts were assessed for protein concentration using a BCA or Bradford protein assay kit (Sigma-Aldrich), respectively.
-
bioRxiv - Microbiology 2020Quote: Oxidized proteins containing carbonyl groups were detected using the OxyBlot Protein Oxidation Detection Kit (Sigma-Aldrich: cat#S7150). Briefly ...
-
bioRxiv - Cancer Biology 2023Quote: ... the protein concentrations were measured using a standard BCA assay with Pierce™ BCA Protein Assay Kit (Sigma). 30 μg of proteins was loaded into a pre-cast gel (Novex™ 16% Tricine Gels ...
-
bioRxiv - Cell Biology 2023Quote: Protein carbonyl formation by oxidative reactions was assessed with the OxyBlot Protein Oxidation Detection Kit (S7150, Merck Millipore), according to manufacturer’s instructions ...
-
bioRxiv - Cancer Biology 2022Quote: ... protein bands were visualized using an enhanced chemiluminescence detection kit (Millipore) and and the Syngene Pxi4 imaging system (Ozyme) ...
-
bioRxiv - Cancer Biology 2020Quote: ... Proteins expression was detected using an enhanced chemiluminescence kit (EMD Millipore).
-
bioRxiv - Molecular Biology 2020Quote: ... Protein synthesis was measured using a DHFR assay kit (Sigma-Aldrich), which measures the oxidation of NADPH (60 mM ...
-
bioRxiv - Physiology 2020Quote: ... Protein concentration was measured using a BCA assay kit (Sigma Aldrich) and spectrometry at absorbance 562nm using a Synergy H4 Hybrid Reader (Biotek) ...
-
bioRxiv - Cell Biology 2022Quote: ... Protein concentrations were determined by Bradford assay kit (Sigma-Aldrich, B6916). Lysates were mixed with 5 × sample buffer (250 mM Tris-HCl ...
-
bioRxiv - Pharmacology and Toxicology 2022Quote: ... as measured by a BCA protein assay kit (Sigma-Aldrich, USA).
-
bioRxiv - Physiology 2022Quote: ... Proteins were visualized using a chemiluminescence (ECL) kit (Millipore, MA, USA). Target protein expression was normalized to β-actin (Sigma-Aldrich ...
-
bioRxiv - Developmental Biology 2022Quote: ... Proteins in gel was visualized with sliver staining kit from Sigma. The gels over 100 KDa (SATB2 molecular weight ...
-
bioRxiv - Biochemistry 2023Quote: ... For labeling an Atto 488 protein labeling kit (Sigma-Aldrich, 38371) and an Atto 647N protein labeling kit (Sigma-Aldrich ...
-
bioRxiv - Microbiology 2019Quote: ... Buffer exchange from glycine-HCl buffer to click reaction buffer (100 mM Na-Phosphate Buffer, pH 7) was performed with Slide-A-Lyzer Dialysis cassettes (Sigma).
-
bioRxiv - Synthetic Biology 2021Quote: ... Linear pST76-A plasmid was ligated with the PCR fragment using T4 ligase enzyme overnight at 16 °C followed by heat inactivation at 65 °C for 10 minutes and dialysis for 1 h on a 50 nm pore-sized membrane (Millipore). The ligation product was transformed into E ...
-
bioRxiv - Bioengineering 2022Quote: ... Each reaction was stirred at room temperature for 24 hours and transferred to a 12,000Da MW cutoff dialysis sack (Sigma Aldrich) and dialyzed in 4L of NaCl solutions of decreasing molarity (0.125M ...
-
bioRxiv - Cell Biology 2022Quote: ... The next day the GelMA solution was dialysed using dialysis with molecular weight cut-off of 12 – 14kDa (Sigma-Aldrich) for up to 5 days against deionised water pH 9.0 ...
-
bioRxiv - Bioengineering 2023Quote: ... The resulting cell-modified graphite electrodes were encased in a dialysis membrane (14 kDa molecular weight pore size, Sigma-Aldrich) that was previously soaked in deionized water for 1 h before insertion in the electrochemical cell ...
-
bioRxiv - Bioengineering 2023Quote: ... solution for 4 h at 60°C and then dialyzed at 4°C against pure water for 4 days using cellulose dialysis tubes (MWCO 3.5 kDa, Sigma Aldrich). Subsequently ...
-
bioRxiv - Biochemistry 2022Quote: ... pH 7.5 and 1 mM TCEP) using small-volume dialysis tubes (D-tube Dialyzer Mini; Millipore Sigma; Burlington MA, USA). Protein concentration was re-measured using the same 1% mass extinction coefficient as described previously ...
-
bioRxiv - Plant Biology 2024Quote: ... the suspension was transferred to dialysis tubing with a 12-kDa MW cut-off (32 mm flat width; Sigma, USA) and stirred overnight ...
-
bioRxiv - Neuroscience 2019Quote: ... Total protein staining solution (REVERT Total Protein Stain kit, LI-COR Inc., # 926-11010; Pyronin Y (Sigma-Aldrich # P9172)
-
bioRxiv - Cell Biology 2021Quote: ... The identical cell lysate was then used for protein concentration determination using Bicinchoninic Acid Protein Assay Kit (Sigma-Aldrich) according to manufacturer’s protocol ...
-
bioRxiv - Cancer Biology 2019Quote: ... and the supernatant was tested for total protein estimate using Total Protein kit from Millipore Sigma (see material section) and cell lysate was frozen at −70°C for electrophoresis where 10µg or 20 µg per sample in sample buffer was heated up at 95°C for 5 min and run in Tris-Glycine gel followed by dry transfer for Western blot detection following the instruction for using iBlot® and/or iBlot®2 Dry Blotting with Blot®2 NC Regular Stacks.
-
bioRxiv - Cancer Biology 2024Quote: Protein-protein interaction was measured by PLA with Duolink In Situ Orange Starter Kit Mouse/Rabbit (DUO92102, Millipore Sigma) according to the manufacturer’s instructions ...
-
bioRxiv - Biophysics 2021Quote: ... pH 7.5 and 150 mM NaCl) and concentration processes performed using a dialysis Amicon Ultra 10 K (Merck Millipore, Darmstadt, Germany).
-
bioRxiv - Microbiology 2021Quote: ... Protein of interest was dialyzed using Slide-A-Lyzer Dialysis Cassette 10,000 MWCO (ThermoScientific, Cat. N° 66810) and concentrated using Amicon Ultra Centrifugal filters 10,000 MWCO (Millipore, Cat. N° UFC901024). Purity was verified by Coomassie staining after SDS-PAGE and concentration was measured by Bradford assay.
-
bioRxiv - Microbiology 2021Quote: ... at a total concentration of 5 μM in 850 μL of media in a 5000 MWCO Pur-A-LyzerTM dialysis tube (Sigma-Aldrich). Previous studies have shown that this concentration of siRNA sufficiently silences gene expression [33–36] ...
-
bioRxiv - Microbiology 2021Quote: ... 2 ml of conditioned medium were dialysed with dialysis tubing cellulose membranes (Sigma-Aldrich, cut-off 12 kDa, REF: D9777-100FT) against 50 ml DTM supplemented with 15 % FBS at 4 °C in two rounds (16 hours ...
-
bioRxiv - Microbiology 2023Quote: ... The OD of the resuspended particles was measured and the particles were transferred to a 14kDa MWCO dialysis tube (Sigma D0405) secured with clips ...
-
bioRxiv - Neuroscience 2023Quote: ... and 50 mM L-Arg and L-Glu by overnight dialysis then concentrated using a 10 kDa Centriprep concentrator (Millipore, UFC901024). The concentrated protein was passed through a pre-equilibrated size exclusion column (Cytiva Custom Superdex TM 75 Increase HiScale TM 16/40 ...
-
bioRxiv - Bioengineering 2023Quote: ... by dialysis using an Amicon® Ultra-15 Centrifugal Filter Unit with 100 kDa molecular weight cutoff regenerated cellulose membrane (Millipore) or via tangential flow filtration (Repligen ...
-
bioRxiv - Molecular Biology 2023Quote: ... Excess stain was removed by dialysis at a 1:1000 ratio against aqueous sea salt solution (40 g L-1, Sigma Aldrich) using dialysis sleeves (14 kDa MWCO ...
-
bioRxiv - Systems Biology 2024Quote: ... either resuspended from a plate or from liquid culture and was further purified by spot dialysis on a nitrocellulose filter (Millipore VSWP02500) against 0.1 mM Tris ...
-
bioRxiv - Microbiology 2021Quote: ... Proteins were concentrated using centrifugal filters and concentrations were measured using the Bicinchoninic acid (BCA) protein assay kit (Millipore Sigma). Proteins were aliquoted and stored at −80°C.
-
bioRxiv - Cancer Biology 2022Quote: An in situ proximity ligation assay was performed to identify protein-protein interactions in cells using a Duolink In Situ Red Starter Kit (Sigma) per the manufacturer’s protocol ...
-
bioRxiv - Evolutionary Biology 2022Quote: ... Protein concentration in the samples was measured using the Bicinchoninic Acid Protein Assay Kit (Sigma Aldrich, St. Louis, MO, USA) according to the manufacturer’s protocol with the following modifications ...
-
bioRxiv - Immunology 2023Quote: ... The protein concentration in the supernatant was measured by the BCA protein assay kit (Sigma-Aldrich, cat. No. BCA1-1KT).
-
bioRxiv - Evolutionary Biology 2019Quote: ... Proteins were visualized after staining with ProteoSilver Silver Stain Kit (Sigma-Aldrich) (S.Fig.1 ...