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Citations for Millipore Sigma :
251 - 300 of 10000+ citations for Granzyme B GZMB Mouse HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: HEK293 cells were cultured in DMEM-F12 (cat n. D6421, Sigma-Aldrich) supplemented with 10% heat-inactivated fetal bovine serum (FBS ...
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MANF regulates unfolded protein response and neuronal survival through its ER-located receptor IRE1αbioRxiv - Cell Biology 2020Quote: HEK293 cells plated on covered with Poly-D-Lysine (P0899, Sigma-Aldrich) coverslips were co-transfected with pEZY BIFC N-Venus and C-Venus plasmids 48 hours after plating ...
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bioRxiv - Neuroscience 2022Quote: ... 0.8×105 HEK293 cells were seeded on poly-L-ornithine (Millipore Sigma) pre-coated 12 mm coverslips ...
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bioRxiv - Cell Biology 2022Quote: HEK293 cells were grown in DMEM 4.5 g/L glucose (D6429, Sigma) supplemented with 10% Fetal Bovine Serum (FBS ...
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bioRxiv - Cancer Biology 2023Quote: ... while HeLa and HEK293 cancer cells were maintained in DMEM (Sigma- Aldrich). Media was supplemented with 10% fetal bovine serum (FBS) ...
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bioRxiv - Biophysics 2023Quote: ... Stable HEK293-6E cell lines were maintained in F17 media (Sigma Aldrich) supplemented with Kolliphor p188 (Sigma Aldrich ...
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bioRxiv - Molecular Biology 2024Quote: HEK293 and Caco-2 cells were grown in DMEM culturing medium (Sigma) with 10% FBS (Gibco ...
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bioRxiv - Cell Biology 2019Quote: ... the membranes were incubated overnight at 4°C with the adequate primary antibodies in TBST buffer: mouse anti-His-tag (1:2000, Millipore, 05-949), rabbit anti-CAP-D2 serum (1:1000 ...
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bioRxiv - Microbiology 2021Quote: ... His-tagged recombinant proteins were detected using an HRP-conjugated anti-His monoclonal antibody (Sigma-Aldrich, St. Louis, MO) according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2024Quote: ... His- and GST-tagged proteins were detected with anti-His (Monoclonal Anti-polyHistidine antibody, Sigma H1029, 1:5000 dilution) or anti-GST (Mouse anti-GST monoclonal antibody ...
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bioRxiv - Cell Biology 2019Quote: ... Primary antibodies used in this work were: mouse anti-α-tubulin clone B-512 at 1:1500 (Sigma Aldrich), mouse anti-EB1 at 1:500 (BD Biosciences) ...
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bioRxiv - Cell Biology 2019Quote: ... blotted and immunodetected with a rat anti-CLASP2 or mouse anti-α-tubulin antibodies (clone B-512, Sigma Aldrich). For analysis of recombinant CLASP2 C-term ...
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bioRxiv - Cell Biology 2021Quote: ... the mouse anti-alpha-tubulin B-5-1-2 was used at a dilution of 1:500 (Sigma, T5168). Polyclonal rabbit anti-TgCentrin1 (Fig ...
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bioRxiv - Biophysics 2023Quote: ... blocked and stained with anti-alpha tubulin primary antibodies (monoclonal mouse clones B-5-1-2 and DM1a, Sigma) overnight at 4°C ...
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bioRxiv - Biochemistry 2022Quote: ... FSL-B(tri) (Function-Spacer-Lipid with blood group B trisaccharide; Sigma Aldrich) or lactosylceramide (LC ...
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bioRxiv - Immunology 2023Quote: ... GC-B cells and follicle B cells were stained with Pna-HRP (Sigma) and anti-mouse IgD-BIOT (SouthernBiotech ...
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bioRxiv - Biophysics 2019Quote: ... as a Tobacco Etch Virus (TEV)-cleavable N-terminal His-tagged (6×-His) fusion protein using a pET46 Ek/LIC vector (Novagen) and purified using Ni-NTA affinity chromatography and gel filtration chromatography as previously described (Hughes et al ...
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bioRxiv - Evolutionary Biology 2021Quote: ... A first purification step was performed using immobilized metal affinity chromatography with a His-bind resin column (His-Bond kit, Novagen) to recover PGM variants ...
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bioRxiv - Biochemistry 2023Quote: ... the protein was eluted in a gradient of His-AC washing buffer and His-AC elution buffer (both supplemented with 0.1% Tween-20 (Sigma Aldrich), 0.1% NP-40 (Thermo Fischer Scientific) ...
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bioRxiv - Plant Biology 2024Quote: ... His–NFR1CD–Myc or His–NFR1CD–K351E–Myc and NopT–FLAG–His were co–expressed by using Duet expressing vectors (Novagen) with 0.5 mM IPTG at 28°C for 18 h ...
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bioRxiv - Biophysics 2021Quote: ... Protein production was confirmed by SDS-PAGE and subsequent Western blotting analysis of a K30 sample after 24 h using an anti-His antibody from mouse (dilution 1:3000, Sigma-Aldrich, cat.no. H1029) and secondary anti-mouse IgG-HRP antibody produced in goat (dilution 1:20,000 ...
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bioRxiv - Cell Biology 2023Quote: ... the membrane was sequentially incubated at room temperature for one hour with anti-His and conjugated anti-mouse antibodies (Sigma-Aldrich, Darmstadt, Germany) (Wu et al. ...
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bioRxiv - Microbiology 2020Quote: ... as a His-tag fusion in pET28b (Novagen). Cells were grown in Terrific-Broth at 37°C to an OD600 = 1.2 and after 15 min at 4°C induced with 1 mM isopropyl β-d-thiogalactoside ...
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bioRxiv - Microbiology 2019Quote: ... Proteins were detected using anti-His-HRP (Sigma) and anti-FLAG-HRP (Sigma ...
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bioRxiv - Plant Biology 2021Quote: ... -His with or without 3-AT (Sigma-Aldrich).
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bioRxiv - Pathology 2021Quote: ... cloned into a pET28a His-tag vector (Novagen) and expressed in E ...
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bioRxiv - Biophysics 2020Quote: ... His-purifying resin was from Novagen (Darmstadt, Germany). Bis-(sulfosuccinimidyl ...
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bioRxiv - Cell Biology 2021Quote: ... and Anti-His(1:3000) antibody(Sigma #H1029).HRP conjugated mouse IgG was used at 1:4000 dilution(Sigma #A3673 ...
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bioRxiv - Neuroscience 2019Quote: ... and His-tagged human HDAC6 protein (EMD Millipore) were incubated with 57 nm 32P-ATP in kinase buffer (50 mM Tris HCl ...
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bioRxiv - Biochemistry 2021Quote: ... anti-His (Sigma H1029; 1:3000 for IB), anti-Hsp70 (Abcam ab47455 ...
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bioRxiv - Plant Biology 2022Quote: ... followed by blotting using Anti-His (SAB4301134, Sigma) and Anti-GST antibody (ab9085 ...
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bioRxiv - Genomics 2023Quote: ... with 10% heat-inactivated (HI) FBS (Sigma F0926). Vero/hSLAM cells were a kind gift from Dr ...
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bioRxiv - Systems Biology 2023Quote: ... MCF7 in DMEM-Hi Glucose medium (Sigma-Aldrich) HCC1937 and ZR-75-1 in RPMI 1640 media (Gibco) ...
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bioRxiv - Neuroscience 2024Quote: ... supplemented with 10% HI-FBS (Sigma-Aldrich, #12306C), 1% GlutaMAX (200 mM ...
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bioRxiv - Neuroscience 2024Quote: ... supplemented with 10% HI-FBS (Sigma-Aldrich, #12306C), 1% GlutaMAX (200 mM ...
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bioRxiv - Neuroscience 2024Quote: ... supplemented with 10% HI-FBS (Sigma-Aldrich, #12306C), 2% GlutaMAX (200 mM ...
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bioRxiv - Cell Biology 2024Quote: ... +10% HI FBS(Sigma Aldrich, cat#F4135-500ML) + penicillin/streptomycin (Sigma ...
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bioRxiv - Biochemistry 2024Quote: ... An anti-His-primary antibody (Sigma, 1:2,000) was incubated with the protein-treated lipid strips to detect Jps1 bound via its C-terminal His-tag ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... human recombinant MAO-A (hMAO-A) and MAO-B (hMAO-B) enzymes (Sigma-Aldrich) were diluted in 50 mM phosphate buffer (final protein amount ...
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bioRxiv - Microbiology 2022Quote: ... purified bacterial cultures (B. adolescentis and B. fragilis at 107CFU/ml) or putrescine (Sigma) diluted to final concentrations of 33/66/100mM ...
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bioRxiv - Cancer Biology 2022Quote: Expression of indicated Flag-tagged CDK6 constructs in HEK293 cells were determined via Western blotting with indicated Flag antibody [mouse anti-FLAG® M2-tag (Sigma-Aldrich, St. Louis, MO, USA, F3165-1MG)] ...
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bioRxiv - Cancer Biology 2021Quote: ... The DNA encoding MHC heavy chain (HLA-C*08:02 and H-2 Kb) and light chain (human β2M and mouse β2M) was synthesized (Idobio) and cloned into pET-22(b) vector (Novagen). The vectors were transformed into the E ...
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bioRxiv - Neuroscience 2021Quote: ... standard Western blot was performed using anti-SST and anti-α-Tubulin (1:8,000, B-5-1-2, mouse monoclonal, Sigma).
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bioRxiv - Cell Biology 2023Quote: ... and incubated as described above with a 0.5 μg/ml mouse α-tubulin antibody (clone B-5 1-2, Sigma) as a loading control.
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bioRxiv - Cell Biology 2024Quote: The following antibodies were used for immunohistochemistry and western blots: mouse anti-tubulin clone B-5-1-2(Millipore Sigma), rabbit anti-CAPS (Abcam EPR15631) ...
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bioRxiv - Neuroscience 2021Quote: HEK293 cells were transfected at 80–90% confluency using PEI transfection reagent (Sigma) with 2 mg/ml PEI per mg of DNA ...
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bioRxiv - Cell Biology 2019Quote: HEK293 cells were transfected with plasmids using polyethylenimine according to manufacturer’s instructions (Sigma). Neurons were transfected by the Ca2+-phosphate method (53) ...
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bioRxiv - Molecular Biology 2021Quote: HEK293 cells were seeded on glass coverslips coated with poly-L-ornithine (Sigma) in clear 12-well plates (3 × 105 cells/well ...
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bioRxiv - Cell Biology 2022Quote: HEK293 Flp-In T-REx stable lines were cultured in DMEM (Sigma, D5671) supplemented with 10% FBS ...
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bioRxiv - Molecular Biology 2022Quote: ... HEK293 cells were transfected with lentiviruses particles containing Pin1 shRNA (Sigma-Aldrich, TRCN0000010577) as per the manufacturer’s instructions ...