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Citations for Millipore Sigma :
251 - 300 of 10000+ citations for Contactin Associated Protein Like 3 CNTNAP3 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2023Quote: ... PREPL proteins were immunoprecipitated using anti-FLAG M2 antibody (Sigma Aldrich) and separated by SDS-PAGE ...
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bioRxiv - Biochemistry 2023Quote: ... Ubiquitinated proteins were detected using an anti-FLAG antibody (F7425, Sigma), HA-TAP-Gb1 and endogenous Gb1-4 were detected with anti-HA (16B112 ...
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bioRxiv - Neuroscience 2024Quote: ... Primary antibodies and biotinylated Hyaluronic Acid Binding Protein (HABP, #385911 Millipore) were incubated for 72 hours at 4°C or 24 hours at room temperature ...
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bioRxiv - Cell Biology 2020Quote: ... Then the cells were washed with PBS 3 times and incubated with cyanine 3 (Cy3)-conjugated donkey anti-goat IgG secondary antibody (1:500, Millipore, Temecula, CA) at room temperature for 1 hour ...
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bioRxiv - Microbiology 2021Quote: ... Immunoprecipitation of 3×Flag-tagged proteins was performed using α-FLAG M2 affinity gel (Sigma-Aldrich) following the manufacturer’s recommendations ...
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bioRxiv - Cell Biology 2021Quote: ... and bound proteins were eluted with 25 µl 1 mg/ml 3 × FLAG peptide (Sigma, F4799) in IP buffer containing 1% NP-40 and 1 × protease inhibitors at 4 °C ...
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bioRxiv - Plant Biology 2020Quote: ... 2 mM EDTA and 1 % [v/v] protein phosphatase inhibitor cocktail 2 and 3 (Sigma-Aldrich)) were added at a concentration of 2 mL/g tissue powder ...
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bioRxiv - Developmental Biology 2021Quote: ... Proteins were immunoprecipitated by the addition of 3 µl of rabbit anti-FGFR3 (Sigma-Aldrich, F0425)/500 µg protein with protein A-agarose (Roche) ...
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bioRxiv - Molecular Biology 2022Quote: ... induction of AID-tagged protein depletion was performed with 3-indole-acetic acid (IAA) (Sigma-Aldrich) during the exponential growth phase (approx ...
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bioRxiv - Cell Biology 2019Quote: ... Silicon wafers were silanized for protein conjugation by incubation with 3-glycidoxy-propyl-dimethoxy-methylsilane (Sigma) (0.045 % in 100 % EtOH ...
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bioRxiv - Biochemistry 2020Quote: ... The purified protein 5-HT2C or HCA2 (3 μg) was immobilized on nickel agarose beads (Sigma) in the incubation buffer containing 50 mM HEPES ...
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bioRxiv - Biophysics 2022Quote: ... Proteins were concentrated using a centrifugal filter unit (3 kDa MWCO, Merck Millipore; Cat. No. UFC900324) at 3500 rpm and 4°C ...
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bioRxiv - Neuroscience 2022Quote: ... The proteins were diluted 1:3 with Urea-buffer (4.5 mM Urea (Sigma-Aldrich Cat#U1250), 10 mM Tris-HCl ...
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bioRxiv - Immunology 2023Quote: ... Expression of the proteins of interest was induced by 3 μg/ml doxycycline (dox, Sigma-Aldrich) added to the culture medium ...
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bioRxiv - Cell Biology 2023Quote: ... TEV-eluted supernatant was added to 100 µL of S-protein agarose (69704-3, EMD Millipore) to capture S-tagged protein ...
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bioRxiv - Immunology 2023Quote: ... Metabolites were separated from proteins and exosomes using a 3 kDa MWCO centrifugation unit (UFC910024, Millipore) and centrifuged at 4000 × g for 40 min at 4°C ...
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bioRxiv - Biochemistry 2024Quote: ... and the bound proteins were eluted with 150 μg/mL 3×Flag (Sigma-Aldrich, Cat# F4799) at 4 °C for 1.5 h ...
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bioRxiv - Cell Biology 2024Quote: ... The proteins were eluted in buffer 5 [buffer 3 plus 200 μM FLAG peptide (Sigma-Aldrich)] for 1.25 hours ...
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bioRxiv - Microbiology 2023Quote: ... Proteins were detected by western blotting using the following antibodies: Anti-GFP antibody (1:3000 dilution; Millipore), anti-aldolase antibody (1:3000 ...
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bioRxiv - Cell Biology 2022Quote: ... The following antibodies were used: PDT-tagged proteins - anti-HA antibody (Sigma, cat# 11666606001, dilution 1:1000), mf-Lon - anti-Flag (Sigma-Aldrich ...
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bioRxiv - Molecular Biology 2023Quote: ... Proteins were detected using commercially available antibodies: anti-Flag mouse Antibody M2 (1:1000, Sigma-Aldrich M8823) and rabbit anti 4R-tau Antibody (1:1000 ...
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bioRxiv - Cell Biology 2020Quote: ... Neutrophil-like HL-60 cells were grown in RPMI1640/glutamate media (Wako 189-02145) supplemented with 12% FBS (Sigma 172012). Differentiated HL-60 cells were obtained by treating the cells with DMSO for 3 days ...
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bioRxiv - Biophysics 2020Quote: African green monkey kidney fibroblast-like cells (COS7, Cell Lines Service GmbH, Eppelheim, #605470) were cultured in DMEM (Sigma, #D8062) containing 10 % FCS (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2021Quote: We investigated dopaminergic neuromodulation of STDP by bath application of specific antagonists for D1-like (SCH23390, SCH; 10 μM, Sigma) and D2-like dopamine receptors (Sulpiride ...
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bioRxiv - Cell Biology 2022Quote: ... and megakaryocyte-like differentiation was induced with 10 nM or 50 nM 12-O-Tetradodecanoyl-phorbol-13 acetate (TPA, Sigma) for 24 hrs ...
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bioRxiv - Biophysics 2019Quote: ... Cells were passaged twice a week and differentiated into a neutrophil-like phenotype by adding 1.25 % (v/v) of dimethyl sulfoxide (DMSO, Sigma-Aldrich) to the cell suspension the first day after passage and ...
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bioRxiv - Cell Biology 2019Quote: ... Then cells were washed with HBSS/Ca/Mg before switching to original media containing the chymotrypsin like fluorogenic substrate LLVY-R110 (Sigma), which is cleaved by proteasome 20S core particle ...
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bioRxiv - Neuroscience 2022Quote: The cellular viability of the brain-like tissues was tested using a lactate dehydrogenase assay (LDH) (Sigma, cat.no. MAK066-1KT) following the manufacturer’s protocol ...
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bioRxiv - Microbiology 2023Quote: THP-1 cells were seeded at 5 ×105/mL in 24 well plate and differentiated to macrophage-like cells by treatment with 200 nM Phorbol 12-myristate 13-acetate (PMA; Sigma) for 18 hrs ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were grown in human plasma-like medium according to the published formulation (15) with 5% dialyzed fetal bovine serum (FBS) (Sigma) and pen/strep (Invitrogen ...
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bioRxiv - Physiology 2023Quote: ... GLP-1 content in the supernatant of T0 and T20 samples was assessed using a Glucagon-Like Peptide-1 (GLP-1) Total ELISA KIT (96-Well Plate Cat. # EZGLP1T-36K, EZGLP1T-36BK, Millipore).
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bioRxiv - Biophysics 2023Quote: African green monkey kidney fibroblast-like cells (COS7, Cell Lines Service GmbH, Eppelheim, #605470) were cultured in DMEM (Sigma, #D8062) containing 10% FCS (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2023Quote: ... Cell lysates and immunoprecipitates were analyzed on Western blots using mouse monoclonal anti-MYC antibody (1:800; Covance) (for exogenous 14-3-3ε) or anti-FLAG antibody (1:800; Sigma) (for exogenous Cdc25B ...
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Faa1 membrane binding drives positive feedback in autophagosome biogenesis via fatty acid activationbioRxiv - Biochemistry 2023Quote: ... and analyzed by SDS-PAGE using mouse anti-GFP antibody (Max Perutz Labs, Monoclonal antibody facility) in 3 % (w/v) milk powder TBST-T (Sigma). Secondary antibody incubation was performed using Dylight 800 α-mouse (Rockland Immunochemicals) ...
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bioRxiv - Cancer Biology 2024Quote: ... About 3x106 cells were immunoprecipitated using 10 µg of a monoclonal SF3B1 antibody (clone 16, MBL D221-3) or 10 µg of the monoclonal U2AF2 antibody (U4759, SIGMA).
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bioRxiv - Cell Biology 2021Quote: Senescence-associated β-galactosidase (SA-β-gal) activity was determined with the Senescence Cells Histochemical Staining Kit (Sigma-Aldrich). Six representative images were captured on a TS100 inverted light microscope (Nikon ...
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bioRxiv - Molecular Biology 2020Quote: ... Singlecell iAT2s were plated on Transwells at a density of 520,000 live cells/cm2 in 100μl of CK+DCI with 10μM Rho-associated kinase inhibitor (“Y”; Sigma Y-27632). 600μl of CK+DCI+Y was added to the basolateral compartment ...
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bioRxiv - Cell Biology 2019Quote: ... and the knee (proximal tibia, distal femur, patella, and associated soft tissues) were dissected out and placed in Ringer’s solution (Sigma) maintained at 25°C with bubbling oxygen with 5% carbon dioxide ...
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bioRxiv - Developmental Biology 2020Quote: ... per manufacturer’s instructions and seeded onto the micropattern at 132,000 – 263,000 cells/cm2 in mTeSR with 10 μM Rho-associated kinase inhibitor (ROCKi Y-27632, Millipore Sigma). After 6h ...
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bioRxiv - Physiology 2022Quote: Senescence-associated β-gal staining was performed with the Cellular Senescence Assay Kit according to the manufacturer’s instructions (Millipore).
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bioRxiv - Genomics 2019Quote: ... RNA-protein complexes were incubated with antibody-bound Protein A Dynabeads (anti-FLAG [Sigma Aldrich F3165] or anti-IgG) at 4C for 2 hours ...
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bioRxiv - Plant Biology 2021Quote: ... and immunoprecipitation of the antibody/protein complexes was performed by adding 50 μl of Protein A-Sepharose (Sigma-Aldrich) made up as a 50% (w/v ...
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bioRxiv - Molecular Biology 2019Quote: ... Cells were then washed 3 times with PBS and incubated with an anti-active Caspase-3 antibody (EMD Millipore, Billerica, MA, 1:200) for 2h at room temperature ...
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bioRxiv - Cell Biology 2020Quote: ... primary antibody after blocking for 1 h at room temperature with 3% BSA (Sigma) in PBS ...
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Obesity alters mobility and adult neurogenesis, but not hippocampal dependent learning in ob/ob micebioRxiv - Neuroscience 2019Quote: ... the solution containing the primary rabbit anti-active Caspase 3 antibody (Merck Millipore, Germany) was applied 1:100 in blocking serum (3 % NGS + 0.1 % Triton X-100 in PBS ...
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bioRxiv - Bioengineering 2019Quote: ... incubated with primary antibody for (Tuj1, Sigma T5076, 1:500 in PBS+3% BSA), washed with PBS 2 x 1h and 1x overnight ...
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bioRxiv - Microbiology 2019Quote: ... for 3 h and then incubated with anti-β1-integrin antibodies (MAB2000 from Millipore) for 1 h ...
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bioRxiv - Microbiology 2021Quote: ... the cells were stained 3 hours with antibodies against γH2AX (1:500, JBW301, Millipore) and S33p-RPA32 (1:500 ...
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bioRxiv - Microbiology 2020Quote: ... Membrane was immunoblotted for ≥ 3 h with primary antibodies : anti-M2 (1:5,000) (Sigma), anti-CtrA (1:5,000) ...
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bioRxiv - Neuroscience 2022Quote: ... for 3-4 hours> Primary antibodies: Rabbit-α-GluA1 (Sigma-Aldrich AB1504, 1:300) and Mouse-α-bassoon (Abcam ab82958 ...