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Citations for Millipore Sigma :
451 - 500 of 10000+ citations for Contactin Associated Protein Like 3 CNTNAP3 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2021Quote: ... oxygenated DAB (3-3’-diaminobenzidine, Sigma-Aldrich) was dissolved in 0.1 N HCl at a concentration of 5.4 mg/ml and subsequently diluted ten-fold into sodium cacodylate buffer (pH 7.4 ...
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bioRxiv - Bioengineering 2022Quote: ... 3-mercaptobenzoic acid (3-MBA, Sigma-Aldrich)-capped gold nanoclusters (3-MBA-Au nanoclusters ...
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bioRxiv - Cell Biology 2020Quote: ... anti-α-adducin phospho S355 antibody (Abmart 20592-1NB-3/C63-S, 1:100, for WB and double transgenics) anti-FLAG antibody (M2, F3165, Sigma-Aldrich 1:100). Myosin Light Chain 2 antibody (ptglab ...
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bioRxiv - Genetics 2022Quote: ... then incubated overnight with appropriate primary antibodies (Supplementary Table 3) in antibody solution consisting of 1xPBS with 1% BSA (Millipore Sigma A4161-1G) and 0.3% Triton X-100 ...
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bioRxiv - Molecular Biology 2019Quote: ... Bound proteins were cleaved off the column with thrombin diluted 1:250 in thrombin cleavage buffer (Novagen #69671-3). The thrombin solution was loaded manually into the column with a syringe ...
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bioRxiv - Biochemistry 2020Quote: ... The protein was then labeled with the MDCC (7-Diethylamino-3-[N-(2-maleimidoethyl) carbamoyl]coumarin) from Sigma-Aldrich. Unreacted MDCC was removed by ion exchange using a Bio-Rex70 column ...
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bioRxiv - Microbiology 2021Quote: ... 50 µg of purified Cu-LpmP or CslZ protein were incubated for 3 h at room temperature with 0.2% peptidoglycan (PG) from Bacillus subtilis (Sigma), 5 mg α-chitin from shrimp shells (Sigma ...
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bioRxiv - Biochemistry 2022Quote: ... The two aqueous streams were supplied with the cell solution and with a 3 μM substrate solution containing lysis agents (0.7× BugBuster protein extraction reagent, Merck Millipore; 60 kU/mL rLysozyme, Novagen) in droplet assay buffer ...
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bioRxiv - Cancer Biology 2021Quote: ... The protein pellet was washed 3 times in a solution of 0.3 M guanidine hydrochloride (Sigma, Cat. No. SRE0066) in 95% ethanol ...
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bioRxiv - Biochemistry 2021Quote: ... The supernatant containing Galectin-3 protein was treated with benzamidine sepharose (HiTrap Benzamidine FF, Sigma, Cat# GE17-5143-02) to bind and remove thrombin ...
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bioRxiv - Plant Biology 2019Quote: ... and bound protein was eluted with 60 μL of 0.5 mg/mL 3×FLAG peptide (Sigma-Aldrich, MO, USA) for 1 h at 4°C ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... and 12 mg RBD protein in 3 mL PBS was mixed with equal-volume Freund’s incomplete adjuvant (Sigma-Aldrich) for the second boosting ...
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bioRxiv - Biophysics 2019Quote: ... Fractions containing the protein were pooled and concentrated by centrifugal concentrators with 3 kDa molecular weight cutoff (EMD Millipore). The sample was polished by gel filtration (120 ml Superdex200 ...
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bioRxiv - Biochemistry 2021Quote: ... Elution fractions containing the protein of interest were concentrated using a centrifugal filter (Amicon Ultra, MWCO 3 kDa, Sigma) before loading onto a size-exclusion Sepharose 200 column (Cytiva ...
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bioRxiv - Biochemistry 2023Quote: ... The eluted protein was concentrated and using using Amicon Ultra-15 centrifugal filters (3 kDa cut-off, Merck-Millipore) and then buffer was exchanged to buffer B (50 mM Tris-HCl pH 8.0 ...
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bioRxiv - Microbiology 2023Quote: ... These lysates were then additionally treated for protein precipitation with 3 volumes of ultra-pure methanol (Sigma-Aldrich, USA). Ultimately ...
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bioRxiv - Biochemistry 2023Quote: ... Proteins were eluted with 200 µL assay buffer supplemented with 0.6 mg∕mL 3× Flag® peptide (Sigma Aldrich), and analysed by Western blotting.
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bioRxiv - Microbiology 2024Quote: ... 150 mM NaCl) in a 1:3 volume ratio for recombinant enterokinase digestion (Novagen, 1 unit/ 50 μg protein) overnight (Gasparian et al. ...
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bioRxiv - Developmental Biology 2021Quote: ... for phosphorylated histone 3 antibody staining (Chen et al., 2020) (pH3, Sigma # 05-806, 1:100), or with Lavdovsky’s fixative (3.7% formaldehyde ...
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bioRxiv - Molecular Biology 2021Quote: ... Primary antibodies (Anti-3-hydroxybutyryllysine [PTM Biolabs 1201], anti-p84 [GeneTex GTX70220], anti-α-Tubulin [Sigma T5168] ...
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bioRxiv - Microbiology 2019Quote: ... and thereafter blocked against non-specific antibody binding with 3% BSA in PBS (Sigma-Aldrich, UK). For staining C ...
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bioRxiv - Neuroscience 2019Quote: ... Antibodies were diluted in 3% normal donkey serum (monoclonal mouse anti-mouse GFAP 1:2000, Sigma G-A-5 ...
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bioRxiv - Microbiology 2020Quote: ... Secondary antibodies were diluted in 3% BSA/PBS containing 1µg/ml Hoechst 33342 (B2261, Sigma-Aldrich), followed by 2×5min washes in 10mM PBS ...
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bioRxiv - Bioengineering 2022Quote: ... Sections were washed 3×5 minutes in TBST buffer and incubated with ChAT antibody (Sigma, AB144) at 1:100 dilutions overnight at 4°C in the blocking buffer ...
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bioRxiv - Developmental Biology 2021Quote: ... in 1XPBS] and immunostained using primary antibodies: mouse anti-Phospho histone-3 (1:200) (Sigma-Aldrich); Rabbit anti-Drosophila caspase-1 (DCP-1 ...
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bioRxiv - Biochemistry 2022Quote: ... Primary antibodies used are as follows: anti-α-tubulin (mouse, 1:3 000, Sigma Aldrich T5168), anti-Ph (rabbit ...
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bioRxiv - Genomics 2022Quote: ... Primary antibodies used were: mouse anti-calbindin (3-11 μg/ml; Sigma Cat# C9848, RRID: AB_476894); and rabbit anti-IBA1 (0.05-0.07 μg/ml ...
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bioRxiv - Microbiology 2022Quote: ... Cap2-3×FLAG was immunoprecipitated using magnetic beads covalently linked to the αFLAG M2 antibody (Sigma). Finally ...
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bioRxiv - Neuroscience 2023Quote: ... sections were incubated with primary antibody for rabbit anti-GluR2/3 (1:100, Millipore, AB1506, RRID:AB_177521) overnight at 4°C and post-fixed with 10% neutral buffered formalin ...
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bioRxiv - Biochemistry 2023Quote: ... Primary antibodies were diluted in 1x TBS-T supplemented with 3% of BSA (Sigma Aldrich, #A7906). HRP-conjugated secondary antibodies were diluted 1:10.000 (anti-Mouse ...
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bioRxiv - Developmental Biology 2023Quote: ... Blocking and antibody incubation was done using 3% bovine serum albumin (BSA) (Sigma-Aldrich A7906-10G) and 0.3% TritonX (Sigma-Aldrich T8787 ...
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bioRxiv - Pathology 2024Quote: ... or an anti 3-NT polyclonal antibody (1:1000, Sigma-Aldrich, St Louis, MO, USA, # N5538) for 90 min ...
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bioRxiv - Microbiology 2019Quote: Macrophage-like phenotype was obtained by treating cells with 25 ng/mL phorbol 12-myristate 13-acetate (PMA, Sigma Aldrich, St-Louis, USA) for 48 h in 24-wells culture plates (Corning ...
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bioRxiv - Zoology 2020Quote: ... Cells were allowed to adhere overnight at 26°C prior to being assessed for β-galactosidase activity associated with cellular senescence using a commercially available Senescence Cells Histochemical Staining Kit (Sigma Aldrich). The manufacturer’s protocol was followed with the following modifications ...
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bioRxiv - Microbiology 2023Quote: ... Single-cell iAT2s were plated on Transwells at a density of 520,000 cells/cm2 in 100 μl of CK+DCI medium containing 10 μM of Rho-associated kinase inhibitor (“Y”; Sigma Y-27632). 600 μl of this medium was added to the basolateral compartment ...
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bioRxiv - Biophysics 2021Quote: ... Protein bands were investigated by using anti-αS monoclonal antibody (Syn 211, Millipore Sigma) and anti-mouse horse radish peroxidase secondary antibody ...
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bioRxiv - Genetics 2021Quote: ... antibodies and precipitated proteins were analyzed by western blot using mouse anti-HA (Sigma) or mouse anti-Flag (Sigma) ...
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bioRxiv - Evolutionary Biology 2020Quote: ... For IP with guinea-pig antibody protein A-conjugated magnetic beads (Merck Millipore, LSKMAGA10) were used ...
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bioRxiv - Microbiology 2020Quote: ... FLAG-tagged ANP32 proteins were detected using mouse anti-FLAG M2 primary antibody (Sigma), followed by goat anti-mouse Alexa Fluor 568 (Invitrogen) ...
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bioRxiv - Cancer Biology 2020Quote: ... and antibody/bead complexes isolated with Magna ChIPTM Protein A+G magnetic beads (Millipore). Complexes were washed ...
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bioRxiv - Plant Biology 2020Quote: ... total proteins were incubated with 50 µL agarose conjugated with anti-Myc antibody (Sigma) for 2 h ...
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bioRxiv - Systems Biology 2021Quote: ... Proteins were detected using a monoclonal anti-FLAG M2 antibody (F3165, Sigma/Merck Millipore) and an HRP-conjugated polyclonal goat anti-mouse antibody (P0447 ...
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bioRxiv - Systems Biology 2021Quote: ... Proteins were detected using a monoclonal anti-FLAG M2 antibody (F3165, Sigma/Merck Millipore) and an HRP-conjugated polyclonal goat anti-mouse antibody (P0447 ...
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bioRxiv - Biochemistry 2020Quote: ... and Flag-tagged FtsX proteins were detected by using ANTI-FLAG® antibody (Sigma) as previously described by Stamsås et al ...
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bioRxiv - Physiology 2021Quote: ... using the following primary antibodies: glial fibrillary acidic protein or anti-GFAP (Millipore, MAB360). Dako Animal Research Kit for mouse primary antibodies (Dako Diagnóstico S.A. ...
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bioRxiv - Microbiology 2021Quote: ... Digested proteins were analyzed by Western blotting with anti-M2 FLAG monoclonal antibody (Sigma).
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bioRxiv - Cancer Biology 2022Quote: ... and antibody/bead complexes isolated with Magna ChIPTM Protein A+G magnetic beads (Millipore). Complexes were washed ...
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bioRxiv - Plant Biology 2021Quote: ... Total protein extracts were incubated with 5 μl T7 antibody (EMD Millipore; Cat # 69522) for 4 h ...
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bioRxiv - Microbiology 2020Quote: ... Proteins were stained using primary antibodies against β-actin (1:10,000, AC-15, Sigma), strep II-tag (1:1,000 ...
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bioRxiv - Developmental Biology 2019Quote: ... Primary antibodies used included anti-rabbit glial fibrillary acidic protein (GFAP, Sigma, 1:400), mouse anti-Zrf1 (Gfap ...