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Citations for Millipore Sigma :
251 - 300 of 319 citations for Canine Parvovirus Antigen since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Synthetic Biology 2022Quote: ... The resulting cells were used to prepare O78 LPS antigen in house by hot phenol water extraction after DNase I (Sigma) and proteinase K (Invitrogen ...
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bioRxiv - Bioengineering 2022Quote: ... and antigen retrieval at 98°C for 15 minutes (KOS microwave tissue processor, Milestone) in citrate buffer pH6.0 (Sigma, C999). Prior to staining ...
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bioRxiv - Biochemistry 2022Quote: ... Then each membrane was incubated with the corresponding primary antibody (mouse IgG anti-Lewis antigen, Sigma–Aldrich, 1:500 dilution) for 1h at 4°C ...
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bioRxiv - Immunology 2022Quote: ... with 1% milk and incubated for 2 hours with the mixture of antigen-coupled microsphere beads in 96-well filter bottom plates (Millipore). After washing with assay buffer ...
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bioRxiv - Molecular Biology 2023Quote: ... For the BrdU staining protocol on tissue section a DNA hydrolysis step directly after antigen retrieval was performed by the addition of 2M HCL (Sigma) in PBS for 30 minutes at 37°C ...
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bioRxiv - Molecular Biology 2023Quote: ... Slides were subsequently rinsed with cold running tap water for 5 min followed by antigen retrieval using citrate buffer at pH 6.0 (Sigma Aldrich). Afterwards ...
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bioRxiv - Immunology 2023Quote: ... parietal-cell antigens were prepared from murine gastric corpus which was digested with 0.05 mg/mL Liberase DH (LIBTM-RO; Sigma-Aldrich) and 0.1 mg/mL DNase I (Roche) ...
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bioRxiv - Cell Biology 2023Quote: ... washed in PBS/0.1 % Triton X-100 and treated with antigen retrieval solution [proteinase K 20 mg/ml (Sigma-Aldrich) dissolved in 10 mM Tris pH 8.0 ...
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bioRxiv - Microbiology 2023Quote: ... with 1% milk and incubated for 2 hours at room temperature with the mixture of antigen-coupled microsphere beads in 96-well filter bottom plates (Millipore). After washing three times with assay buffer ...
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bioRxiv - Neuroscience 2023Quote: ... spinal cord sections underwent antigen retrieval at 85 °C for 10 min with 1X citrate buffer pH 6.0 (Sigma-Aldrich). Tissue sections were washed with PBS and permeabilised with 1% Triton-X-100 in PBS for 30 min at RT ...
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bioRxiv - Physiology 2024Quote: ... followed by rehydration with different % of ethanol (From 100 % to 70 %, 111000190, Pharmco) and the antigen retrieval step with citric buffer (pH 6, C9999, Sigma) in heat for 20 min ...
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bioRxiv - Cell Biology 2020Quote: ... hydrated sections were subjected to antigen retrieval by pronase (1 mg/ml in PBS containing 5 mM calcium chloride; Sigma-Aldrich) incubation for 10 minutes at room temperature ...
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bioRxiv - Molecular Biology 2021Quote: Soluble egg antigen (SEA) of LE was extracted by mechanical lysis (Funakoshi motorized pestle; Diagnocine, Hackensack, NJ) in RIPA buffer (Millipore Sigma) supplemented with protease inhibitors (Protease Inhibitor Cocktail I ...
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bioRxiv - Immunology 2022Quote: ... Sections were washed 3 times in PBS followed by antigen retrieval for 20 minutes with steam using 1X Citrate buffer (Millipore, 21545), pH=6.0 ...
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bioRxiv - Immunology 2022Quote: ... Slides were subsequently boiled with antigen retrieval buffer (Tris-EDTA, pH9, made in house or citrate buffer, pH6, Sigma C999-1000mL) and blocked for one hour with PBS containing 1% bovine serum albumin (BSA) ...
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bioRxiv - Cancer Biology 2019Quote: ... the TMA slide was subjected to Cell Conditioning 1 (CC1, Ventana) antigen retrieval and stained with a prediluted anti-MCT antibody (Mouse, Clone G3, Millipore #MAB1222) using an automated immunostainer ...
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bioRxiv - Immunology 2022Quote: ... Endogenous peroxidase activity was blocked with 3% hydrogen peroxide for 10 min and antigen retrieved using proteinase K (Sigma-Aldrich, P6556) in Tris-EDTA pH8 buffer for 4 min at room temp ...
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bioRxiv - Developmental Biology 2021Quote: ... boiled in citric acid (pH 2.0) for antigen retrieval and stained with an α-MHC antibody (Sigma My-32, 1:1000) or α-GFP antibody (Abcam ab13970 ...
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bioRxiv - Microbiology 2020Quote: Infected cells grown on coverslips were fixed with 4% paraformaldehyde and permeabilized/blocked with a Triton-X100 (0.2%)/BSA (3%) solution and then incubated with mouse anti-Flavivirus Group Antigen 4G2 (Millipore, Burlington, MA), mouse anti-alphavirus capsid (kindly provided by Dr ...
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bioRxiv - Physiology 2020Quote: ... 5 μm tissue sections were subject to antigen retrieval (0.01 M citric acid and 0.1% Triton X) (Sigma, St. Louis, MO), blocked with the avidin/biotin blocking kit (Vector Laboratories) ...
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bioRxiv - Molecular Biology 2021Quote: ... Anti-flavivirus group antigen antibody that probes ZIKV E protein and anti-SARS-CoV-1/2 NP 1C7C7 antibody was purchased from Sigma-Aldrich. Anti-influenza A virus nucleoprotein (NP ...
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bioRxiv - Cancer Biology 2020Quote: ... Tissue sections were subjected to a heat-induced antigen retrieval step prior to exposure to primary OSM antibody (1:50, HPA029814, Sigma-Aldrich). Immunodetection was performed using the Roche Ventana BenchMark ULTRA IHC/ISH staining system ...
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bioRxiv - Molecular Biology 2020Quote: ... pH 8 and the α-NOCT antibody was antigen affinity purified with NOCT (64-431) immobilized on Immobilon-P PVDF membrane (Millipore). Guinea pig α-NOCT was eluted from the membrane with 0.1 M glycine and 20% glycerol ...
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bioRxiv - Genetics 2022Quote: Longitudinal paraffin-embedded sections were processed as described (Gomez et al., 2022) following 1-hour antigen retrieval with 2mg/ml Hyaluronidase (Sigma-Aldrich) at 37°C ...
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bioRxiv - Microbiology 2022Quote: ... PyVAN was documented by immunohistochemical staining with mouse monoclonal anti-SV40 T Antigen (clone PAb416, Sigma Aldrich - Saint-Quentin-Fallavier France) diluted 1 ...
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bioRxiv - Developmental Biology 2023Quote: ... rat anti-PDGFRβ (eBiosciences 14-1402-81), rabbit anti-AQP3 (Alomone Labs, AQP-003, after antigen retrieval) rabbit anti-Runx1 (Sigma, HPA004176), rabbit anti-IRF9 (Cell Signalling ...
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bioRxiv - Genetics 2023Quote: ... Antigen retrieval was performed by pressure cooker incubation of slides in 1L of pre-warmed 1X Citrate Buffer (Sigma Aldrich #C9999) + 0.1% Tween in dH2O for a total of 15 mins (human ...
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bioRxiv - Microbiology 2023Quote: ... at 15 days and 36 days with 1:1 (v/v) mixture of antigen with incomplete Freund’s adjuvant (Sigma-Aldrich, USA). After the first immunization ...
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bioRxiv - Cancer Biology 2023Quote: ... Tissue sections were immediately subjected to heat-mediated antigen retrieval at 95°C for 10 minutes in 1X pH6.0 sodium citrate buffer (Sigma-Aldrich, #21545) using Micromed T/T Mega Multifunctional Microwave Rapid Histoprocessor (Milestone) ...
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bioRxiv - Immunology 2023Quote: Eosinophilic airway inflammation was induced by two intraperitoneal injections of 5μg of cockroach antigen (CA, Greer Laboratory, Lenoir, NC) mixed in 100μl of alum adjuvant (aluminum hydroxide, Sigma, St. Louis, MO) at day 0 and 7 ...
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bioRxiv - Cancer Biology 2023Quote: ... followed by antigen retrieval using Tris EDTA buffer pH 9 (Genemed, 10-0046) or 1x sodium citrate at pH 6 (Sigma, C9999) according to manufacturer’s recommendation for specific antibodies ...
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bioRxiv - Neuroscience 2023Quote: ... coverslips were first incubated with Tris-glycine solution 5min at room temperature followed by blocking of non-specific antigens with a cold-water fish blocking buffer (Sigma-Aldrich) for 30min ...
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bioRxiv - Neuroscience 2021Quote: ... tissues underwent antigen retrieval by incubating for 15 minutes in hot 10mM sodium citrate buffer pH=6.0 (Sigma cat. no. S-4641). Sections were rinsed in PBS and incubated in 3% H2O2/PBS for 15 minutes ...
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bioRxiv - Immunology 2019Quote: ... sections from the initial portions of sockets were incubated with primary antibody for Proliferating Cell Nuclear Antigen (PCNA) (#MAB424, Merck Millipore, Darmstadt, Germany) diluted to 1:150 in blocking solution and incubated at 4°C overnight ...
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bioRxiv - Developmental Biology 2022Quote: ... Antigen retrieval was performed using 1X Sodium Citrate Buffer diluted from a 10X stock (10X Stock: 100mM trisodium citrate (Sigma, Cat#S1804), 0.5% Tween 20 (Thermo Fisher ...
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bioRxiv - Cancer Biology 2020Quote: ... Gels were blotted onto nitrocellulose membranes and probed for each antigen using the following antibodies at the indicated dilutions: Human PEAK1 (Millipore 1:400), Mouse PEAK1 (Abgent 1:400) ...
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bioRxiv - Immunology 2020Quote: ... Detection of GFP and Iba1 required high-temperature antigen unmasking in 0.01 M citrate buffer (pH 6.0) (Sigma-Aldrich, San Diego, CA). Sections were treated with biotin and streptavidin blocking solutions (Vector Laboratories ...
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bioRxiv - Neuroscience 2019Quote: ... pre-incubation of the neurotransmitter antibodies used in this study with their corresponding antigen (100 μM - 400 μM; GABA (Sigma-Aldrich, A2129), glutamate (Sigma-Aldrich ...
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bioRxiv - Microbiology 2020Quote: ... whilst H antigen was detected with 1 μg/ml Ulex Europaeus Agglutinin I (UEA-I) lectin labeled with fluorescein isothiocyanate (Sigma-Aldrich, Germany). Lea and Lex were detected with 0.5 μg/ml of mAbs MEM-158 and 7Le (Sigma-Aldrich ...
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bioRxiv - Immunology 2020Quote: ... The polyreactivity of hybridoma-obtained IgA was tested by adding them to ELISA plates that were coated with common antigens dissolved in PBS before coating ELISA plates: Lipopolysaccharide (Sigma-Aldrich; L4391), double stranded DNA (Sigma-Aldrich ...
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bioRxiv - Pathology 2022Quote: ... and a mouse monoclonal antibody raised against Simian Virus-40 (SV-40, Macaca mulatta polyomavirus-1, Betapolyomavirus macacae) LT antigen (CalBiochem®, Clone PAb416, Sigma-Aldrich) was used as primary antibody at a 1:200 dilution ...
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bioRxiv - Immunology 2022Quote: ... The moANCA518 binding to the antigen was detected after incubation for 1 hour at RT of alkaline phosphatase conjugated goat anti-human IgG (Sigma, A-9544) antibody in a 1:10,000 dilution in TBS (20 mM Tris ...
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bioRxiv - Microbiology 2023Quote: Proteins in the cell and viral lysates (5 μg of total protein and 10 ng of p24 antigen) were separated by SDS-PAGE and transferred to PVDF membranes (Millipore, Cat# IPVH00010). Membranes were blocked with 5% milk in PBS containing 0.1% Tween 20 (0.1% PBST ...
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bioRxiv - Immunology 2023Quote: ... Antibody binding to S1 and RBD antigen was detected using a three-step approach consisting of anti-mouse or antirat Fc biotin (Sigma Aldrich B7401) followed by streptavidin-HRPO (Life Technologies cat# SA1007 ...
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bioRxiv - Immunology 2023Quote: ... The first kit used was the Milliplex® SARS-CoV-2 antigen panel 1 IgA assay (Millipore Sigma, Cat No. HC19SERA1-85K) with the Wuhan-1 strain RBD and N proteins included ...
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bioRxiv - Immunology 2023Quote: ... Culture supernatants were screened using ELISA (see below) and antigen-reactive clones were expanded in RPMI-1640 complemented with 10% IgG-free Fetal Calf Serum (Sigma-Aldrich, #F1283) into roller bottles (Sigma-Aldrich ...
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bioRxiv - Microbiology 2022Quote: ... The major components of the assay included: 1) rS-RBD coating of plates as target antigen (2ug/ml in Sigma Carbonate-Bicarbonate Buffer); 2 ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... cells producing HCMV specific antigens were detected 24h post infection by immunoperoxidase staining using monoclonal antibodies directed against the UL123-coded 72 kDa immediate early antigen 1 (IEA1) (Mouse Anti CMV IEA, MAB8131, Millipore, Temecula, CA, USA) and 120h post-infection by immunoperoxidase staining using monoclonal antibodies directed against UL55-encoded late antigen gB (LA ...
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bioRxiv - Neuroscience 2021Quote: ... subjected to heat induced antigen retrieval in a citrate buffer and incubated with a mouse anti-NeuN antibody (clone A60, Millipore, MAB377, 1:100). The used secondary antibodies were mouse linker antibody (Abcam ...
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bioRxiv - Microbiology 2022Quote: ... Expression of recombinant antigens in induced and un-induced controls was assessed by immunoblot using a monoclonal anti-poly- histidine antibody (Millipore-Sigma, diluted 1:2000) after SDS-PAGE ...