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Citations for Millipore Sigma :
201 - 250 of 319 citations for Canine Parvovirus Antigen since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2020Quote: ... the antigen was detected using mouse-anti-polyHis conjugated with horseradish peroxidase (HRP) (1:20000, A7058, Sigma) for His-tagged antigens ...
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bioRxiv - Biochemistry 2020Quote: ... for mFc tagged antigen versions or goat-anti-hIgG(Fc) conjugated with HRP (1:70000, A0170, Sigma) if hFc-tagged antigens had to be detected ...
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bioRxiv - Molecular Biology 2022Quote: ... Antigen-antibody complexes were identified using goat anti-mouse AP (1:10,000 dilution of A2179, Sigma-Aldrich) and CDP-Star™ Substrate (T2146 ...
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bioRxiv - Developmental Biology 2022Quote: ... antigen unmasking was performed either in 10 mM sodium citrate buffer (pH 6.0; S1804-500G, Sigma-Aldrich) for 12 min at 97°C ...
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bioRxiv - Neuroscience 2023Quote: ... underwent antigen retrieval for 30 minutes at 98°C in citrate buffer (10 mM citrate, Sigma-Aldrich) 0.05% Tween 20 (pH 6.0 ...
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bioRxiv - Developmental Biology 2023Quote: ... antigen retrieval was performed by boiling the sections in 10 mM sodium citrate and 0.05% Tween20 (Sigma) for 10 min ...
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bioRxiv - Microbiology 2023Quote: ... the antigen-serum-bead mixture was transferred into a 96-well filter HTS plate (Millipore, Bedford, MA). The plate was washed 8 times with Buffer A followed by twice with PBS on a vacuum manifold as described previously [26] ...
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bioRxiv - Microbiology 2023Quote: ... rodentium antigens were produced by homogenization of cultured bacterial pellet in lysis buffer (CellLytic B, Sigma-Aldrich) followed by centrifugation and harvesting of the soluble protein antigens ...
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bioRxiv - Molecular Biology 2021Quote: ... sonicated and then incubated with Pol III antibodies recognizing antigen POLR3A (18) or H3K4me3 antibodies (05-1339, Millipore). Immunoprecipitated DNA was used to generate sequencing libraries using the Takara SMARTer ThruPLEX DNA-seq Kit according to the manufacture’s protocol ...
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bioRxiv - Immunology 2019Quote: ... the total soluble tachyzoite antigen (STAg) fraction was loaded into a lactose column (Sigma-Aldrich, St. Louis, MO) and equilibrated with PBS containing 0.5 M NaCl ...
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bioRxiv - Neuroscience 2020Quote: ... Antigen retrieval was performed by heating the sections in 10 mM citrate buffer (pH 6.0, Sigma-Aldrich, USA) at > 96 °C for 20 minutes ...
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bioRxiv - Immunology 2021Quote: ... mice were primed in one hind footpad with 25mg antigen emulsified in Hunters Titermax Gold adjuvant (Sigma-Aldrich). At day 10 ...
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bioRxiv - Zoology 2019Quote: ... Antigens and PBS were mixed with equal volumes of complete Freund’s adjuvant (Sigma-Aldrich, St. Louis, MO, USA) on day 0 and with equal incomplete Freund’s adjuvant (Sigma-Aldrich ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... The antigen-antibody complexes were detected using peroxidase-conjugated goat anti-human or goat anti-mouse IgG (Sigma) and revealed using the enhanced chemiluminescence (ECL ...
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bioRxiv - Microbiology 2021Quote: ... 500 antigen-conjugated microspheres per well were added into a MultiScreen Solvinert 96 Well Filter Plate (Merk Millipore) and incubated at room temperature with plasma samples diluted in a 1:100 ratio ...
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bioRxiv - Cell Biology 2022Quote: ... Antigen retrieval was performed for 30 min in citrate buffer (Sigma-Aldrich, St. Louis, MO, C9999, 1:10). Sections were incubated with 3% hydrogen peroxidase (Sigma-Aldrich ...
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bioRxiv - Immunology 2022Quote: ... Samples were first fixed in methanol in −20°C then treated with Antigen Retrieval Buffer (pH 6, Sigma). 0.3% Triton X-100 (Sigma ...
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bioRxiv - Immunology 2023Quote: ... tetanus antigen samples were prepared by desorption with buffered Ethylenediaminetetraacetic acid (EDTA) (Sigma-Aldrich Chemie GmbH Taufkirchen Germany). Diphtheria antigen samples were prepared by desorption with sodium citrate (Sigma-Aldrich Chemie GmbH Taufkirchen ...
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bioRxiv - Developmental Biology 2023Quote: ... Antigen retrieval was conducted using a citrate buffer (created in house from Citric Acid (Sigma-Aldrich, C-0759) and Sodium Citrate (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2023Quote: ... Sections prepared for Alexa Fluor™ antibodies were heated in 1x Citrate Antigen retrieval (Cat #c9999, Millipore Sigma) before blocking (5% Donkey serum cat#NC1697010 ...
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bioRxiv - Cell Biology 2024Quote: ... cover slips were incubated with the primary antibody (SV40 T antigen mouse-anti-human, Merck-Millipore, 1:50) overnight at 4 °C in a humidifier chamber ...
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bioRxiv - Neuroscience 2024Quote: ... the retina was first treated with pre-heated (about 70 °C) 1× antigen retrieval citrate buffer (Sigma, C9999), then followed the same protocol as with the brain section described above ...
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bioRxiv - Molecular Biology 2021Quote: ... The antigen-antibody complexes were visualized using a secondary horseradish peroxidase-conjugated goat anti-mouse IgM antibody (Sigma-Aldrich) at a dilution of 1:10,000 ...
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bioRxiv - Developmental Biology 2021Quote: ... For staining on paraffin sections antigen retrieval was performed by either 1mg/ml trypsin (Sigma, T7168, St. Louis, MO) or 10 μg/ml Proteinase K for 20 minutes at 37°C ...
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bioRxiv - Developmental Biology 2021Quote: ... Antigen retrieval was performed by treatment of tissue sections with porcine trypsin tablets (Cat No. T7168-20TAB, Sigma-Aldrich) for 10 min at room temperature ...
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bioRxiv - Immunology 2021Quote: ... Labeled antigens were sterilized by filtering through 0.22 µm pore size Spin-X centrifuge tube filters (CLS8160, Millipore Sigma) and stored at 4°C until use ...
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bioRxiv - Immunology 2019Quote: ... scFv secretion levels were analyzed by ELISA after coating with HEL antigen (Sigma-Aldrich, 62971, 4 μg ml-1) in PBS (Thermo ...
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bioRxiv - Cancer Biology 2021Quote: ... Tumor proliferation in the R26-GB2/MMTV-PyMT animals was assessed by PCNA (proliferating cell nuclear antigen) immunostaining using the MAB424R antibody (1:10,000; clone p10, Millipore). Briefly ...
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bioRxiv - Microbiology 2020Quote: ... was used to detect the different antigens with anti-rabbit secondary antibodies conjugated to horseradish peroxidase (1:25,000; catalog # A0545, Sigma). Either photographic film or a ChemiDoc Imaging System (Bio-Rad ...
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bioRxiv - Cancer Biology 2020Quote: ... Tissue sections were deparaffinized and antigen retrieval was achieved by use of heat-induced epitope retrieval with pH 6.0 citrate buffer (Sigma). Tissue sections were stained with H&E ...
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bioRxiv - Immunology 2020Quote: ... were cultured in 24 well plates and stimulated with inactivated SARS-COV-2 antigen (1.2 μg/ml) or PMA (25 ng/ml, cat # P8139; Sigma) and Ionomycin (1 μg/ml ...
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bioRxiv - Immunology 2020Quote: ... total IgG against these antigens were detected using HRP-conjugated respective anti-mouse antibody (Sigma-Aldrich, eBioscience and Invitrogen) and the reaction was detected using TMB substrate (BD biosciences) ...
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bioRxiv - Pathology 2022Quote: ... Enzymatic antigen retrieval was performed for 10 minutes in a 37°C water bath using pepsin (Sigma-Aldrich, P7000). After rinses ...
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bioRxiv - Bioengineering 2023Quote: ... Sections (5 μm) were deparaffinized and rehydrated and subjected to antigen retrieval using citrate buffer (0.0126 M citric acid, Sigma cat ...
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bioRxiv - Microbiology 2023Quote: Sections were deparaffinized in xylene and rehydrated in a descending ethanol series prior to heat-induced antigen unmasking in Citrate Buffer pH 6.0 (Sigma). After blocking of nonspecific binding in PBS-T containing 10% goat serum and 1% BSA ...
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bioRxiv - Developmental Biology 2023Quote: ... 5μm E14.5 paraffine sections underwent deparaffinization and microwave antigen retrieval in pH 6.0 sodium citrate with 0.05% Tween-20 solution (Sigma-Aldrich), followed by gradual chilling ...
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bioRxiv - Developmental Biology 2023Quote: ... Tissue cross-sections were deparaffinized and rehydrated and subjected to antigen retrieval using citrate buffer (0.0126 M citric acid, Cat. No. C-0759, Sigma; 0.0874 M sodium citrate ...
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bioRxiv - Bioengineering 2023Quote: ... The antigen-retrieval buffer used for live-cell lysis was 2× Tris-glycine buffer (Sigma Aldrich, St. Louis, MO) supplemented with 1% SDS and 12 M urea (Sigma Aldrich ...
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bioRxiv - Genetics 2023Quote: Rehydrated histological sections underwent antigen retrieval by incubation with 1 mg/mL pepsin in 0.5 M acetic acid (Millipore) at 37 °C for 30 min ...
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bioRxiv - Immunology 2021Quote: ... MAb was reacted with antigen coated on the plates and then treated with serially diluted C1q from human serum (Sigma). C1q binding was detected with horseradish peroxidase-conjugated anti-C1q antibody ...
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bioRxiv - Microbiology 2022Quote: ... immunization was continued in bi-weekly intervals by subcutaneous and intraperitoneal injection of 20-30 μg of antigen preparations formulated with Ribi Adjuvant System (RAS, Sigma) according to manufacturer instructions ...
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bioRxiv - Microbiology 2020Quote: ... Mice were injected intraperitoneally with 100 µL chyle containing 10-50 µg antigen prepared with TiterMax Gold adjuvant (Sigma-Aldrich) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... except that the primary antibody for viral detection was a monoclonal antibody against the coronavirus group antigen (Milipore Sigma #MAB9013) and the secondary was a horseradish-peroxidase-conjugated goat-anti-mouse antibody (enQuire BioReagents # Q2AB1).
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bioRxiv - Microbiology 2022Quote: ... the Milliplex SARS-CoV-2 Antigen Panel 1 IgG was used according to the manufacturer’s instructions (Merck Millipore, Darmstadt, Germany).
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bioRxiv - Developmental Biology 2021Quote: ... sections were subject to 0.1M citrate antigen retrieval at 95°C for 10 mins before the primary antibody for type-II collagen (Millipore, MAB8887), ACAN [30] or lubricin (Abcam ...
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bioRxiv - Immunology 2021Quote: ... Cocalico Biologics administered vaccines by first priming with 50µg of either the single-antigen cocktail of nanoparticles or mosaic nanoparticles in TiterMax Gold (Sigma-Aldrich) and boosting with 25µg of either for-mu-lation every 3 weeks for a total of 3 boosts after prime ...
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bioRxiv - Immunology 2021Quote: ... with 1% milk and incubated for 2 hours with the mixture of antigen-coupled microsphere beads in 96-well filter bottom plates (Millipore). After washing with assay buffer ...
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bioRxiv - Developmental Biology 2020Quote: ... Cryostat sections or whole embryos were stained using standard protocols and antibodies against the following antigens: HA (1:250, Sigma), β-catenin (1:300 ...
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bioRxiv - Immunology 2022Quote: ... with 1% milk and incubated for 2 hr with the mixture of antigen-coupled microsphere beads in 96-well filter bottom plates (Millipore). After washing three times with assay buffer ...
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bioRxiv - Developmental Biology 2022Quote: ... Blocking of unspecific antigens was carried out by incubation in 3% bovine serum albumin (BSA; Sigma-Aldrich; dissolved in PBST) for 1 h at RT ...