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Citations for Millipore Sigma :
251 - 300 of 10000+ citations for 5 Nitro 1H benzo de isoquinoline 1 3 2H dione since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... HEK 293FT cells were transfected with 1μg of TRC-pLKO.1-Puro plasmid containing either non-targeting shRNA (5’-CAACAAGATGAAGAGCACCAA-3’) or ATF4-targeted shRNA (5’-GCCTAGGTCTCTTAGATGATT-3’) (Sigma-Aldrich), together with 1 μg mixture of packaging plasmids (pMD2.G and psPAX2 ...
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bioRxiv - Neuroscience 2021Quote: ... antisense: 5’-ACCGAUUGAAGUUAAUGUC-3’), Myef2 (sense: 5’-CUUUGGUGGAGUUGGCCGA-3’, antisense: 5’-UCGGCCAACUCCACCAAAG-3’) or siRNA universal negative control (SIC001, Sigma-Aldrich) were purchased from Sigma-Aldrich ...
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bioRxiv - Synthetic Biology 2020Quote: ... and blocked for 1h in 3% BSA (Fisher bioreagents CAS 9048-46-8) and 0.1% Triton X-100 (Sigma 9002-93-1). The cells were then incubated in primary antibody overnight ...
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bioRxiv - Neuroscience 2022Quote: ... neurons were treated 1h with 1 µM swinholide A (Sigma), 3 h with 5 µM cucurbitacin E (Sigma) ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... dexamethasone (1 μM for 1h or 24h, Sigma–Aldrich, #D4902) or L-arginine (0.5mM for 1h ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... The cell viability was evaluated by using the 3-(4,5-Dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) cell proliferation assay (Sigma). Non-treated cells were used as control ...
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bioRxiv - Genetics 2021Quote: ... Primary antibodies used: anti-DES (1:1000; D8281, Sigma), anti-beta-actin (1:1000 ...
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Large-scale conformational changes of FhaC provide insights into the two-partner secretion mechanismbioRxiv - Biophysics 2022Quote: ... 1g/L 15N,2H-isogro (Sigma) and 13C-α-ketobutyric acid (Sigma ...
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bioRxiv - Bioengineering 2020Quote: ... in 1x PBS and exposed for 1h to a 3% Bovine Serum Albumin (BSA, Sigma Aldrich) blocking solution in 1x PBS.
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bioRxiv - Molecular Biology 2021Quote: ... for 1h and subsequently developed using 2,2-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) substrate (Sigma). Absorbance was measured at 405nm in a BioTek Synergy HTX multi-mode reader ...
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bioRxiv - Microbiology 2021Quote: ... A carboxylated indene standard (1H-Indene-3-carboxylic acid) was purchased from Sigma-Aldrich (catalog #MNO000013). Hexanes and acetone (Fisher) ...
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bioRxiv - Immunology 2022Quote: ... or MDM (106) were treated for 1h at 37°C with 1 ml of VLPs-VPX+ containing 5 μg/ml polybrene (Sigma, #H9268) for SAMHD1 degradation16,37 before the addition of 20 µg CAp24 of each control or/and encoding-gene lentiviral vectors with 5 μg/ml polybrene for 2h transduction ...
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bioRxiv - Immunology 2022Quote: ... were coated with respective antigen for 2h at RT and then blocked with 5% BSA (Sigma-Aldrich). 50 μl 1:50 diluted plasma sample or monoclonal Abs was added to the wells and incubated overnight at 4°C ...
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bioRxiv - Cancer Biology 2022Quote: ... N-methyl-N’-nitro-N-nitrosoguanidine (MNNG; Sigma-Aldrich), and bleomycin (Pharmachemie) ...
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bioRxiv - Immunology 2021Quote: PBMCs and tissue MNC suspensions were treated with 5 μM Chk2 inhibitor II or 2-[4-(4-chlorophenoxy)phenyl]-1H-benzimidazole-5-carboxamide (Sigma) prepared in final volume of 1% bovine serum albumin (Sigma-Aldrich ...
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bioRxiv - Systems Biology 2022Quote: ... of 5,5’dithiobis-2-nitrobenzoic acid conversion to 2-nitro-5-thiobenzoic acid in the presence of Coenzyme A thiol generated during citrate production (CS0720, Sigma) as previously described.62
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bioRxiv - Physiology 2023Quote: ... of 5,5’dithiobis-2-nitrobenzoic acid conversion to 2-nitro-5-thiobenzoic acid in the presence of Coenzyme A thiol generated during citrate production (CS0720, Sigma) as previously described (Crouch et al. ...
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bioRxiv - Microbiology 2022Quote: ... 3-Methyl-1-phenyl-2- pyrazolin-5-one (Edaravone; Millipore 443300) was resuspended in ethanol at 500mM and heated at 65°C for solubilization ...
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bioRxiv - Cell Biology 2023Quote: ... 5-fluorouracil (5-FU) : 3 µM (F6627; Sigma), cisplatin (CDDP ...
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bioRxiv - Neuroscience 2023Quote: ... for 2h before clearing in benzyl alcohol:benzyl benzoate (1:2) (Sigma-Aldrich-Merck). Samples were stored in this solution at room temperature and protected from light.
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bioRxiv - Molecular Biology 2023Quote: The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium Bromide (MTT) cell growth kit (CT02, Millipore, MA, USA) was used for measuring cell viability ...
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bioRxiv - Neuroscience 2023Quote: ... The cell viability was measured by quantifying the activity of cellular dehydrogenases that reduce MTT (3-4,5-dimethyl thiazolyl-2,5-diphenyl-2H-tetrazolium bromide, Sigma Inc.) to a purple formazan salt [35] ...
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bioRxiv - Systems Biology 2023Quote: ... Sections were then blocked for 1h with 3% (v/v) donkey serum (Sigma-Aldrich, St Louis, MO) diluted in TBS-T wash buffer ...
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bioRxiv - Cell Biology 2021Quote: ... and anti-alpha-Tubulin-FITC (1:200, 1h, Millipore Sigma #F2168). Coverslips were mounted in Vectashield medium with DAPI (Vector Laboratories #H-1200) ...
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bioRxiv - Molecular Biology 2023Quote: ... siZWINT (Sigma, 5’-GCACGUAGAGGCCAUCAAA-3’). RNAiMAX (Invitrogen ...
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bioRxiv - Bioengineering 2023Quote: ... The neuromodulators used are 6-cyano-7-nitroquinoxaline-2,3-dione (CNQX, Sigma-Aldrich) 50 µM ...
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bioRxiv - Biophysics 2020Quote: ... and LMB 20 nM 2h (Sigma Aldrich).
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bioRxiv - Bioengineering 2021Quote: ... 2H-Perfluoro decyl triethoxysilane (PFDTES, Sigma-Aldrich) for 3 hours ...
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bioRxiv - Biochemistry 2022Quote: ... Cytidine 3′, 5′ cyclic monophosphate (3′, 5′ cCMP) and Cytidine 2′, 3′ cyclic monophosphate (2′, 3′ cCMP) were purchased from Sigma-Aldrich (Bangalore, India) and used without further purification ...
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bioRxiv - Neuroscience 2023Quote: ... followed by a blocking step for 1h with 5% normal donkey serum (NDS) (D9663, Sigma Aldrich) in 0.1% PBS-Triton X-100 (PBS-T) ...
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bioRxiv - Cell Biology 2024Quote: ... Blocking was performed for 1h using 5% dried milk or Bovine Serum Albumin (Sigma-Aldrich #A9647) in TBS-T (50mM Tris-HCl pH 7.5 ...
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bioRxiv - Genetics 2023Quote: ... Beads were then incubated in 400 μl of Benzo buffer containing 2,500 units of benzonase (Sigma-Aldrich) for 1 h at 4°C and washed three times in IP buffer ...
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bioRxiv - Microbiology 2024Quote: ... Benzo[a]pyrene 10 mM stocks were made with 100% dry dimethylsulfoxide (DMSO) (Sigma-Aldrich, 96% purity). This BaP stock was water-bath sonicated for 20 minutes to ensure proper dissolution ...
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bioRxiv - Developmental Biology 2021Quote: ... and then in Nitro Blue Tetrazolium (Sigma-Aldrich, N6639-1G) and 5-bromo-4-chloro-3-indolyl phosphate (Sigma-Aldrich ...
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bioRxiv - Physiology 2022Quote: ... Nω-nitro-L-arginine methyl ester (L-NAME) (Sigma-Aldrich) was added to the drinking water (0.5 g/L ...
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bioRxiv - Molecular Biology 2021Quote: ... 6-thioguanine or N-methyl-N’-nitro-N-nitrosoguanidine (Sigma) were performed the next day for 24h before drug retrieval ...
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bioRxiv - Pathology 2023Quote: ... For L-NAME (NG-nitro-L-arginine methyl ester) (Sigma) treatment ...
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bioRxiv - Molecular Biology 2022Quote: ... Oligonucleotides 5’-GAAAAAAAAAATATACGCTAAGATTTTTGG-3’ and 5’-ATGACTAAACCCCCCCTCC-3’ synthesized and HPLC-purified by Sigma-Aldrich were used ...
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bioRxiv - Physiology 2024Quote: ... CDH1 Forward 5′-TTACTGCCCCCAGAGGATGA-3′ and Reverse 5′-TGCAACGTCGTTACGAGTCA-3′;) were purchased from Sigma-Aldrich. mRNA expression was determined using the comparative 2-ΔΔCt method and normalized to the mRNA expression level of endogenous references (PPIA ...
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bioRxiv - Biophysics 2024Quote: ... respectively: EcoEF7,3_For: 5’-GCACTATTTGCTATATATTGTGTGGTTGAATCTTTTTTCAACTACATCTAGTATCTC-3’ and EcoEF7,3_Rev: 5’-GAGATACTAGATGTAGTTGAAAAAAGATTCAACCACACAATATATAGCAAATAGTGC-3’ were ordered from Sigma-Aldrich, resuspended and annealed in buffer containing 10 mM Tris pH 7 ...
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bioRxiv - Neuroscience 2020Quote: ... and Fibronectin for >1h (Sigma, F1141 ...
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bioRxiv - Neuroscience 2020Quote: ... sections were incubated for 2h at room temperature with Hoechst (Sigma, B2261, 1/50.000) and secondary antibody ...
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bioRxiv - Neuroscience 2020Quote: ... sections were incubated for 2h at room temperature with Hoechst (Sigma, B2261, 1/50.000) and secondary antibodies in blocking solution ...
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bioRxiv - Immunology 2023Quote: ... PBMC were stimulated for 2h with Phorbol myristate acetate (PMA) (Sigma, 1 µg/µL), Ionomycine (Sigma ...
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bioRxiv - Synthetic Biology 2024Quote: ... Sections were incubated with 1% 2,3,5‐triphenyl‐2H‐tetrazolium chloride solution (TTC, Sigma-Aldrich) for 30 minutes at 37°C in the dark and washed with PBS ...
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bioRxiv - Physiology 2023Quote: ... glycogen phosphorylase was inhibited by IV (jugular vein) injection of 1-(3-(3-(2-Chloro-4,5-difluorobenzoyl)ureido)-4-methoxyphenyl)-3-methylurea (Sigma, 5 mg/kg) one hour prior to the start of a tracer infusion ...
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bioRxiv - Microbiology 2022Quote: ... polymyxin B sulfate and 2-Heptyl-3-hydroxy-4(1H)-quinolone (PQS) (Sigma-Aldrich Corp., St. Louis, MO) were dissolved in water ...
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bioRxiv - Cell Biology 2023Quote: ... 3 mg of proteins were subsequently incubated for 1h with 10 μl of FAM134B or FAM134C antibodies (Sigma Prestige-HPA012077 and -HPA016492 ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Bixafen, (N-(3’,4’-dichloro-5-fluorobiphenyl-2-yl)-3-(difluoromethyl)-1-methylpyrazole-4-carboxamide) (Bixafen, (Sigma-Aldrich, St ...
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bioRxiv - Neuroscience 2023Quote: ... specimens were blocked 2h with 5% normal goat serum (NGS) in 0.3 % PBT (1x PBS with 0.3 % Tritonx-100; Sigma Aldrich). Incubation with primary antibodies (Tab.2 ...