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Citations for Millipore Sigma :
2901 - 2950 of 10000+ citations for 6 Chloro 7H pyrrolo 2 3 d pyrimidine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... supplemented with 1.5 mM 3-amino-1,2,4-triazol (3-AT, Sigma). Selection plates were left for 8 days at 30°C ...
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bioRxiv - Synthetic Biology 2023Quote: ... activated with 250mM 1-Ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDC, Sigma)/ N-Hydroxysuccinimide (NHS ...
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bioRxiv - Molecular Biology 2023Quote: ... 3-[(3-Cholamidopropyl) dimethylammonio]-1-propanesulfonate hydrate (CHAPS) (C3023; Sigma Aldrich), and sodium dodecyl sulfate (SDS ...
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bioRxiv - Systems Biology 2024Quote: ... or 3-bromopyruvate (3-BP; Sigma-Aldrich, St. Louis, MO; 16490) were added to their respective wells ...
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bioRxiv - Neuroscience 2022Quote: ... and Mannitol (3 g of Bacto Peptone [Becton Dickinson], 5 g of Yeast Extract [Sigma-Aldrich], 25 g of D-Mannitol [Sigma-Aldrich], 1 L of Milli-Q water). Liver Infusion Broth (Becton Dickinson) ...
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bioRxiv - Bioengineering 2021Quote: ... was used to stain the sections for EdU and 4′,6-diamidino-2-phenylindole (DAPI; #D9542; 1:1000 dilution; Sigma-Aldrich, St. Louis, MO, USA). Briefly ...
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bioRxiv - Cancer Biology 2020Quote: ... the target cells were trypsinized and replated in 6-cm dishes in media containing 2 μg/mL Puromycin (P8833, Sigma-Aldrich Quimica S.A., Madrid, Spain). Selection media was changed every 48-72 h for a minimum of 1 week before cell lines were frozen in 50% FBS and 10% DMSO and stored in liquid nitrogen for later use.
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bioRxiv - Neuroscience 2022Quote: ... To assess either apoptotic and necrotic cell death pycnotic nuclei were identified with 4’,6-diamidino-2-phenylindole (DAPI) staining (Cat# D9542-5MG, Sigma-Aldrich, St Louis, MO, USA).
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bioRxiv - Bioengineering 2024Quote: ... BSA (1%) and PBS-T (0.5%) followed by counterstaining with 4’,6-diamidino-2-phenylindole (DAPI) in PBS (1x) (Sigma-Aldrich, St. Louis, MO, 1:1000) for 20 minutes at RT ...
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bioRxiv - Bioengineering 2023Quote: ... the nuclei were stained at room temperature for 15 minuts with 4’,6-diamidino-2-phenylindole (DAPI)(Sigma Aldrich, D9542, 1:500, 1 mg/ml) to yield a blue nucleic fluorescence by binding to nucleic acids.
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bioRxiv - Neuroscience 2023Quote: ... Cells were stained with DAPI before mounting with Mowiol (12 mL of 0.2 M Tris buffer pH8.5, 6 mL distilled water, 6 g glycerol, 2.4 g Mowiol 4-88, Merck Millipore).
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bioRxiv - Neuroscience 2023Quote: ... Coverslips were shortly rinsed in distilled water and mounted using Mowiöl (12 ml of 0.2 M Tris buffer, 6 ml distilled water, 6 g glycerol, 2.4 g Mowiol 4-88, Merck Millipore). Samples were imaged directly or within the next 48h ...
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bioRxiv - Neuroscience 2023Quote: ... 50 mM 6-OHDA exposure were made from a stock solution of 100 mM 6-OHDA (Sigma-Aldrich) in 20 mM L-Ascorbic Acid (AA) ...
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bioRxiv - Biochemistry 2024Quote: ... Fixed cells were stained with DAPI and mounted on Mowiöl (12 ml of 0.2 M Tris buffer, 6 ml distilled water, 6 g glycerol, 2.4 g Mowiol 4-88, Merck Millipore) for imaging.
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bioRxiv - Cancer Biology 2024Quote: ... (2) 2% Alconox (Sigma 242985 ...
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bioRxiv - Cancer Biology 2023Quote: ... (2) 2% Alconox (Sigma, 242985 ...
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bioRxiv - Cell Biology 2020Quote: Cultured CT26 cells were collected and their viability was assayed by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT, Sigma, St. Louis, MO, USA) colorimetric method ...
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bioRxiv - Neuroscience 2019Quote: ... BW723C86 (α-methyl-5-(2-thienylmethoxy)-1H-Indole-3-ethanamine monohydrochloride) and a primary antibody raised against β-actin were purchased from Sigma (USA). Other primary antibodies ...
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bioRxiv - Biophysics 2021Quote: ... Membranes were washed with PBST 2-3 times and bands were visualized upon addition of SigmaFast BCIP®/NBT tablets (Sigma B5655) (Fig ...
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bioRxiv - Neuroscience 2021Quote: ... CS03iCTRn2 hPSCs were dissociated with Versene and colonies were transferred to an ultra-low attachment T-25 flask containing EZ sphere culture medium (a mixture of DMEM and F-12 medium in a 7:3 ratio supplemented with 1× B-27 supplement minus vitamin A [Life Technologies], 2 µg/mL heparin [Sigma-Aldrich] ...
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bioRxiv - Biochemistry 2020Quote: ... The cells were restreaked onto SD/-Trp/-Leu and incubated at 30 °C for 2–3 days before performing a colony-lift β-galactosidase assay (X-gal, Sigma-Aldrich) according to the Yeast Protocols Handbook (PT3024-1 ...
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bioRxiv - Cell Biology 2021Quote: ... Oocytes were surgically removed from anesthetized frogs (40, 46) under anesthesia (2 g/L Tricain, 3-aminobenzoic acid ethyl ester, Sigma A-5040). Preparation of defolliculated oocytes was carried out as described in (106) ...
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bioRxiv - Microbiology 2020Quote: ... 200 μL of freshly made 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT; 1 mg/mL; Sigma-Aldrich, St. Louis, MO) in serum-free ...
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bioRxiv - Biophysics 2020Quote: ... oocytes were incubated twice (1st 45 min, 2nd variable) in OR-2 containing 3 mg/ml collagenase type IV (Sigma, C-9891). Defolliculated oocytes were stored in ND96 solution containing (in mM) ...
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bioRxiv - Cancer Biology 2022Quote: ... 0.2 mg/mL sheared salmon sperm DNA and 2 mg/mL BSA) containing 50 ng of or HLA-B-biotin DNA probe (5’ TGTCCTAGCAGTTGTGGTCATCGGAGCTGTGGTCGCTGCTGTGAT-biotin 3’) (Sigma-Aldrich, USA) in a humidified chamber overnight ...
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bioRxiv - Biochemistry 2019Quote: ... All other chemicals including palmitate and 3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium Bromide) (MTT) were obtained from Sigma-Aldrich (St. Louis, USA).
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bioRxiv - Cancer Biology 2021Quote: ... Viable cells were determined post treatment using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) (Sigma-Aldrich, USA; Cat# M2128) assay by measurement of absorbance at 570 nm.17 The relative rate of cell growth for each cell line was factored into the analysis by subtracting the absorbance at time 0 from both the control and treatment groups ...
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bioRxiv - Immunology 2022Quote: ... type 2 and type 3 were mixed and subsequently concentrated using 10 kDa Amicon® Ultra Centrifugal Filters (Merck Millipore, Billerica, MA) to a nominal concentration of 10000-16000-32000 DU/mL.
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bioRxiv - Plant Biology 2022Quote: Kinase activity assay was carried out by mixing 2 μg of CORK1KD or GST-CORK1KD-G748E with 3 μg of myelin basic protein (MBP; Sigma-Aldrich, Germany) in kinase reaction buffer (25 mM Tris-HCl ...
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bioRxiv - Cell Biology 2024Quote: ... logarithmically growing vps4Δ CDC48 or cdc48-3 cells were shifted to 37 °C for 2 h and simultaneously treated with 50 µM MG-132 (Sigma, Cat. # 474787) or the respective vehicle ...
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bioRxiv - Molecular Biology 2024Quote: ... The number of viable cells was determined by mitochondrial conversion of 3-(4, 5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) (Sigma Aldrich, Missouri, USA) to formazan ...
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bioRxiv - Cancer Biology 2024Quote: ... 1.8 µg pCMV-VSV-G (lentiviral envelope plasmid) and 2 µg purified shRNA DNA with 12 µg (3:1) of X-tremeGENE transfection reagent (Sigma Aldrich, #6366244001). Following 72 hours of transfection ...
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bioRxiv - Neuroscience 2023Quote: ... The cells were washed 3 times with 1x PBS and incubated for 2 hours with 5% goat serum (NGS -Sigma-Aldrich, G9023) in 1x PBS with 0.3% Triton (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2022Quote: ... Samples were then cleared by replacing ethanol with ethyl cinnamate (ECi; Ethyl 3-phenyl-2-propenoate; Sigma-Aldrich, order no. 112372-100G). After 30-60 min ...
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bioRxiv - Biophysics 2023Quote: ... The surface of the coverslips was functionalized by amine using the silanization solution of N-(2-aminoethyl)-3-aminopropyltrimethoxysilane (Sigma-Aldrich, 8191720100), acetic acid ...
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bioRxiv - Molecular Biology 2023Quote: ... The locus with expected insertion was amplified by PCR using 5 μL KOD Hot Start 2× Master Mix (Sigma Aldrich 71842-3), 0.2 μL each primer at 10 μM ...
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bioRxiv - Biophysics 2023Quote: ... the sample was resuspended in 100 μL 1 x DPBS and incubated for 1 hour in the dark with ∼ 3 μL of the MPM-2 antibody (anti-phospho-Ser/Thr-Pro, Cy5 conjugate) (Millipore, Sigma-Aldrich, USA) that stains cells containing mitosis-specific phophorylations ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: Cellular dehydrogenase activity was analyzed by the conversion of 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) (Sigma Aldrich, St. Louis, MO) as described.[22e ...
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bioRxiv - Cell Biology 2021Quote: ... cells expressing D-amino acid oxidase (DAO) were treated with 10 mM D-alanine (Sigma-Aldrich, #A7377) before the internalization or secretion assays were performed ...
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bioRxiv - Microbiology 2023Quote: ... in 10 mL TSB-D supplemented with 25 µg/ml chlorophenol red-β-d-galactopyranoside (CPRG, Sigma). An OG1RF pCJK205 monoculture (inoculated at 108 CFU/mL ...
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Recruitment of the m6A/Am demethylase FTO to target RNAs by the telomeric zinc finger protein ZBTB48bioRxiv - Molecular Biology 2024Quote: Actinomycin D treatment was carried out with 5 μg/mL Actinomycin D (Sigma-Aldrich catalogue number A1410) for the indicated times prior to RNA extraction ...
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ITPK1 is an InsP6/ADP phosphotransferase that controls systemic phosphate homeostasis in ArabidopsisbioRxiv - Plant Biology 2020Quote: ... 0.04 mg L−1 6-benzylaminopurine (Sigma), 0.02 mg L−1 indole acetic acid (Sigma) ...
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bioRxiv - Cell Biology 2020Quote: ... and 6 mg/ml fetuin (Sigma-Aldrich). After microinjection and IBMX release as described above ...
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bioRxiv - Cell Biology 2020Quote: ... using 6% concanavalin A-Type IV (Sigma). Live-cell imaging were performed using a DeltaVision RT wide-field fluorescence imaging system (Applied Precision ...
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bioRxiv - Evolutionary Biology 2019Quote: ... 0.1 units glucose-6-phosphate dehydrogenase (Sigma), and 350 μM substrate in 100 μL reaction volume ...
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bioRxiv - Developmental Biology 2021Quote: ... 6 mg/ml glucose (Sigma, Cat# G7528), 0.1% NaHCO3 (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2020Quote: ... anti-Glucose-6-phosphate dehydrogenase (Sigma ImmunoChemicals), and anti-Imp4 (S ...
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bioRxiv - Neuroscience 2019Quote: ... 6 mg ml-1 glucose (Sigma-Aldrich), and 1 % Pen/Strep (Life Technologies) ...
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bioRxiv - Neuroscience 2019Quote: ... and 6 units/ml Nystatin (Sigma N1638). Membrane inserts were handled with sterile forceps and the medium was changed 100% 4 hours after plating and at 4 div ...
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bioRxiv - Cell Biology 2019Quote: ... 100 mM 6-deoxyglucose (D9761, Sigma Aldrich) from a 1 M stock in H2O in glucose free DMEM (11966025 ...