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Citations for Millipore Sigma :
2901 - 2950 of 10000+ citations for 4' Trifluoromethoxy 5 trifluoromethyl 1 1' biphenyl 3 carboxylic acid since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2024Quote: ... 1% antibiotic-antimycotic and 1μM trans retinoic acid (Cat. No. 554720, Sigma-Aldrich, Oakville, ON, CA). Cells were maintained in fresh differentiation media daily for 10 days.
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bioRxiv - Cell Biology 2020Quote: ... washed in running tap water for 5 min and stained in Biebrich Scarlet-Acid Fucsin for 5 min (#HT151, Sigma). Sections were rinsed in de-ionized water and re-fixed in freshly made Phosphomolybdic/Phosphotungstic/dH2O (1:1:2 ...
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bioRxiv - Cancer Biology 2024Quote: ... the cultures were washed in cold PBS then the cells were scrape harvested in 300 μL 5% 5-sulfosalicylic acid (Sigma) in water and stored at -20°C for a maximum of 72 hours ...
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bioRxiv - Cell Biology 2022Quote: ... Lipids were separated using a solvent mixture of triethylamine/chloroform/ethanol/water (5:5:5:1, v/v) (all solvents HPLC grade, Sigma-Aldrich, USA) as mobile phase in a solvent vapour saturated twin trough chamber (CAMAG ...
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bioRxiv - Cell Biology 2023Quote: ... FAIK3-5 were treated with 1 µM of the hypomethylating agent 5-Azacytidine (5-Aza, Sigma-Aldrich, St. Louis, MO, United States) in an additional approach ...
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bioRxiv - Molecular Biology 2021Quote: ... pombe (strain SHY1058) cultures were labeled with 5 mM 4-thiouracil (4-thioU) (Sigma-Aldrich #440736) for 4 min ...
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bioRxiv - Cell Biology 2020Quote: ... The surfaces were then incubated with 5% (3-Aminopropyl)trimethoxysilane (Sigma) in ethanol for 5 min ...
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bioRxiv - Developmental Biology 2020Quote: ... CHIR99021 (3 μM) and LY411575 (5 μM, Sigma-Aldrich, no. SML0506). Culture media was changed every other day.
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bioRxiv - Neuroscience 2021Quote: ... as control siRNA for luciferase was used (5’-UAAGGCUAUGAAGAGAUAC-3’; Sigma). Immediately before transfection 2×106 cells were seeded in 6-well plates in 1.4 ml of medium containing 10% fetal serum ...
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bioRxiv - Bioengineering 2021Quote: ... 5□μL 3-aminopropyltriethoxysilane (APTES) 99% (Sigma-Aldrich cat. no. 440140) was added ...
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bioRxiv - Bioengineering 2022Quote: ... The PDMS parts were submerged in a 5% 3-aminopropyltriethoxysilane (Sigma) solution to generate surface amine ...
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bioRxiv - Neuroscience 2021Quote: ... reversely transfected (human siTDP 5’-GCAAAGCCAAGAUGAGCCU-3’, Sigma-Aldrich or siLUC) and harvested 48 h later ...
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bioRxiv - Molecular Biology 2022Quote: ... (5’-CACGGGACAGCCTGAGCGGAACGGTGCTAATCGTGCGGT-3’) strand of e5 probe were synthesized (Sigma-Aldrich) and sense strand was end labelled with [γ32P] ATP using T4 polynucleotide kinase according to manufacturer’s protocol ...
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bioRxiv - Neuroscience 2021Quote: ... 40 nM of siTDP (mouse siTDP 5’-CGAUGAACCCAUUGAAAUA-3’, Sigma-Aldrich) or non-targeting siLUC (5’-UAAGGCUAUGAAGAGAUAC-3’ ...
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bioRxiv - Molecular Biology 2024Quote: ... 0.1 mM 8-Bromoadenosine 3′,5′-cyclic monophosphate sodium salt (Sigma), and 0.1 mM IBMX (Sigma ...
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bioRxiv - Neuroscience 2023Quote: ... CHIR99021 (3 μM) and LY411575 (5 μM, Sigma-Aldrich, no. SML0506)].
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bioRxiv - Microbiology 2023Quote: ... 2’,3’,5’-Triacetyl-6-azauridine (azaribine; Sigma-Aldrich, Cat. # T340057), and brequinar sodium salt hydrate (brequinar ...
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bioRxiv - Genomics 2022Quote: ... PNP-GMP (guanosine 5′-[β,γ-imido]triphosphate, 3 mM, Sigma) was included in the lysis buffer of TIS(Ret ...
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bioRxiv - Genetics 2023Quote: ... and 1µM adenosine 3’,5’-cyclic monophosphate (cAMP)(Millipore Sigma, #A9501). To aid in cell atachment ...
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bioRxiv - Immunology 2023Quote: ... We implanted 5×105 EO771 (ATCC) or AT-3 (EMD Millipore) mouse mammary tumor cell lines and 1×105 mouse mammary fibroblasts orthotopically into 4th inguinal mammary fat pads of 6-8-week-old female C57BL/6J female mice (The Jackson Laboratory)(46) ...
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bioRxiv - Cell Biology 2023Quote: ... cloned 5’-NdeI to 3’-NotI into pET21a(+) (Novagen, Madison, WI) (pET21a-APOL7C::FLAG::1XHis) ...
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bioRxiv - Cancer Biology 2024Quote: ... 2.5 µM Oligo-dT primer (5’-AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT-TTVN-3’, Sigma-Aldrich)) using FACSAria Fusion cytometer (BD Biosciences ...
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bioRxiv - Immunology 2024Quote: ... centrifuged for 5 min at 500 g (Centrifuge 3-18KS, Sigma), then resuspended in full media to a concentration of 1 × 106 cells/mL and distributed in 96-well plates (100 μL/well) ...
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bioRxiv - Cancer Biology 2024Quote: ... Small interfering RNAs (siRNAs) included Non-targeting: 5’-GAUCAUACGUGCGAUCAGATT-3’ (Sigma), NT5E ...
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bioRxiv - Cell Biology 2024Quote: ... Faf2-targeting (5’-AGAAAGCGAAGCTCCCTTTTGG-3’, Millipore Sigma, Sanger library clone HS5000006660) AGPS-targeting (5’-ATAAAAATGGTAACACCTAG-3’ ...
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bioRxiv - Cell Biology 2024Quote: ... for 3 h or 5 μM ionomycin (Sigma-Aldrich Cat#407950) for 15 min prior to imaging ...
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bioRxiv - Cell Biology 2022Quote: ... To induce the auxin-degron system in DLD-1 cells, 500 µM indole-3-acetic (A10556, Fisher) and 500 µg ml−1 doxycycline (Sigma, D9891) were added to the media and cells incubated for 24 h.
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bioRxiv - Cell Biology 2021Quote: ... Samples were incubated at 37 °C for 30 min to achieve maximum polymerization and then were mixed in a 1:1 volume ratio with pre-warmed PM buffer containing 3 mM MgCl2 and 2% Methylcellulose (MO512, Sigma-Aldrich). Final concentrations of protein ...
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bioRxiv - Neuroscience 2021Quote: ... Brains were homogenized in 3:1 (v/w) of PBS containing 1% Triton X-100 supplemented with protease inhibitors (cOmplete™, Sigma) using the TissueRuptor (Qiagen ...
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bioRxiv - Cell Biology 2021Quote: ... Pellets were resuspended in urea buffer (50mM Tris HCl pH 7.5, 5mM EDTA, 6M Urea, 1% SDS, 1mM PMSF, 1:100 Phosphatase Inhibitor Cocktail 3 [Sigma-Aldrich P0044]) and cells were lysed by bead beating ...
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bioRxiv - Biochemistry 2022Quote: ... they were cultivated 72 hours in the presence of 2 μM DL-threo-1-phenyl-2-palmitoylamino-3-morpholino-1-propanol (PPMP) (Sigma-Aldrich) to inhibit the synthesis of glucosylceramide-based GSLs 75 and incubated with fluorescent SaroL-1 as previously described ...
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bioRxiv - Neuroscience 2021Quote: ... plus 5% normal goat serum for 1 hr at RT before 3 d incubation in primary antibody diluted in block (1:1,000, chicken anti-GFP, Abcam, ab13970; 1:200 rabbit anti-cleaved caspase 3, EMD Millipore, AB3623). Sections were washed several times in TBS ...
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bioRxiv - Neuroscience 2020Quote: ... Tissue samples from brain and peripheral organs were homogenized in 3:1 (v/w) of PBS containing 1% Triton X-100 supplemented with protease inhibitors (cOmplete™, Sigma) using the TissueRuptor (Qiagen ...
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bioRxiv - Microbiology 2020Quote: ... Cleaned tissue samples were incubated at 37 °C for 1 h in 10 mL DPBS with 1 mM DTT and 3 mM EDTA (Sigma-Aldrich). After incubation ...
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bioRxiv - Cancer Biology 2020Quote: ... pelleted, and re-suspended in lysis buffer (1 mM CaCl2, 3 mM MgCl, 1mM EDTA, 1% Triton X-100 (Sigma-Aldrich), 10 mM TrisHCl ...
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bioRxiv - Microbiology 2021Quote: ... Allicin was synthesized by oxidation of 3-[(Prop-2-en-1-yl)disulfanyl]prop-1-ene (diallyl disulfide, Sigma-Aldrich, Germany) with peracetic-acid (glacial acetic acid/H2O2 ...
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bioRxiv - Bioengineering 2022Quote: ... A stock solution of 3 mg/mL rat tail collagen type I was neutralized with 1% 1 M NaOH (Sigma, S5761) and 10% 10x phosphate buffered saline ...
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bioRxiv - Microbiology 2024Quote: ... parasite pellets were placed on ice for 5 min before addition of 50 μl chloroform followed by 200 μl methanol:ultrapure water (3:1, including scyllo inositol as an internal standard, 1 nmol, Sigma-Aldrich, I8132). Extraction was facilitated through vigorous vortexing ...
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bioRxiv - Cancer Biology 2023Quote: ... supplemented with fresh protease inhibitors (3 µg/mL Leupeptin, 1 µg/mL macroglobulin, 1 µM benzamidine, 0.5 µM PMSF) and phosphatase Inhibitors (EMD Millipore, 524625). Cell lysates were incubated 10 minutes on ice and centrifuged at 13000 rpm for 10 minutes ...
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bioRxiv - Developmental Biology 2023Quote: ... 10 mM NaCl, 3 mM MgCl2, 0.1% Tween-20, 1% BSA, 1 mM DTT, 0.1 U/µl Sigma Protector RNase Inhibitor) was then added to the cell lysis and the nuclei were pelleted by centrifuging at 500xg for 5 minutes at 4°C ...
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bioRxiv - Systems Biology 2023Quote: ... 6 M Urea and 1% Sodium Dodecyl Sulphate supplemented with 100mM phenylmethylsulfonyl fluoride and 1% phosphatase inhibitor cocktail 3 (Sigma-Aldrich)) ...
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bioRxiv - Cancer Biology 2024Quote: ... followed by dilution of the supernatant with fresh medium in a ratio of 1:1 and addition of 3 µg/mL hexadimethrine bromide (polybrene, Sigma-Aldrich). 5 µg/mL Puromycin were added and maintained to select for positively transduced cells ...
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bioRxiv - Biochemistry 2024Quote: ... were homogenized using a Polytron in 1 ml of 20 mM sodium acetate buffer containing 0.15 M NaCl and 1% Zwittergent 3-14 detergent (Millipore-Sigma 693017), pH 4.75 ...
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bioRxiv - Microbiology 2024Quote: ... The cells were maintained with DMEM+10%FCS for 3 hours and fixed with acetone/methanol (1:1 ratio) and stained with Giemsa stain (Sigma-Aldrich). Stained cell images were taken by EVOS XL imaging system (Life Technologies).
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bioRxiv - Synthetic Biology 2024Quote: ... filtered spent media collected from a confluent flask) at the ratio of 1:3 to fresh medium with 25% FBS and 1% penicillin-streptomycin (Penicillin-Streptomycin, Sigma Aldrich). Confluent wells were then assessed with fluorescence microscopes ...
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bioRxiv - Microbiology 2024Quote: ... 2 μg each Spike and Nucleocapsid R203M mutant expression vectors [29,30] using X-tremeGENE 9 DNA Transfection Reagent at 1:3 ratio in serum-free Optimem and 1 hour incubation at room temperature (Millipore Sigma). The supernatant was replaced 4-6 hours post-transfection with 1 mL fresh medium ...
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bioRxiv - Biochemistry 2024Quote: ... 2% H2O (MilliQ) and were shaken for 3 h before precipitation with 45 mL of ice cold 1:1 diethyl ether (Sigma-Aldrich) and hexanes (Sigma-Aldrich) ...
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bioRxiv - Immunology 2024Quote: ... and the second block was for 1 hr with 1× TBS IHC Wash Buffer with Tween 20 with 3% (v/v) normal donkey serum (Sigma-Aldrich), 0.1% (v/v ...
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bioRxiv - Cell Biology 2024Quote: ... The anti-TXNIP (VDUP1) (mouse mAb, MBL, K0205-3 1:1000) and anti-beta actin (rabbit, Sigma Aldrich, A2066, 1:5000) antibodies were used for target protein detection ...
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bioRxiv - Molecular Biology 2021Quote: ... to YPD containing 5 mM 4-thiouracil (Sigma, 440736-1G). Cells were incubated with 4tU for 5 minutes before pelleting (one minute ...