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Citations for Millipore Sigma :
2851 - 2900 of 4279 citations for Recombinant Human ABHD15 His tagged since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: 100 μg of recombinant Tau 2N4R were incubated for 2h at room temperature in 100 mM sodium citrate pH 6 (Sigma, cat. no. W302600), 100 mM sodium cyanoborohydride (Sigma ...
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bioRxiv - Immunology 2020Quote: Data was generated from immunizations of three juvenile New Zealand white rabbits: two rabbits with recombinant Escherichia coli Beta-gal (β-galactosidase from Sigma Aldrich, Inc.), and one rabbit with two linear peptides RNSEEARTDRPSQQLRSLNGE (peptide A ...
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bioRxiv - Biophysics 2021Quote: ... The recombinant expression constructs were transfected into CHO Tet-On cells and expression was carried out in the presence of biotin as previously described.18 Culture medium containing secreted recombinant A1 or A1A2A3 protein was collected and concentrated five times using an Amicon Ultra-15 Centrifugal Filters (EMD Millipore, Billerica, MA). Free biotin was removed from the samples using a desalting PD-10 column (GE Healthcare ...
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bioRxiv - Neuroscience 2022Quote: Organotypic entorhino-hippocampal tissue cultures (≥ 18 days in vitro) were treated with mouse recombinant Tumor Necrosis Factor α (60 pM or 6nM; Sigma, Cat# T7539) for 24h ...
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bioRxiv - Biochemistry 2024Quote: ... pCD44 was generated by pretreatment of recombinant murine CD44-Fc-AviTag with CK2α in PBS with 10 mM MgCl2 and 1 mM ATP (Sigma Aldrich, A1852-1VL). DNA was harvested from the libraries before and after sorting using Zymoprep Yeast Plasmid Miniprep II (Zymo Research ...
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bioRxiv - Bioengineering 2023Quote: ... ethylenediaminetetraacetic acid (EDTA), iodoacetamide (IAM), urea, recombinant porcine trypsin, K2IrCl6, and phosphate buffered saline (PBS, pH 7.4) were purchased from Sigma-Aldrich (St. Louis, MO). L-histidine monohydrochloride monohydrate and L-histidine were purchased from Avantor (Allentown ...
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bioRxiv - Microbiology 2024Quote: ... for NF10 production or with 50 μg of each of five recombinant LMWs in alum mixed with 200 ng/mouse pertussis toxin (Sigma-Aldrich, MO, USA) for QD8 production ...
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bioRxiv - Biochemistry 2024Quote: ... 0.35 mg of recombinant ixochymostatin was coupled with 0.1 mg of Sepharose 4B CNBr-activated resin following the manufacturer’s instructions (Sigma-Aldrich, St. Louis, MO, USA). Subsequently ...
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bioRxiv - Bioengineering 2021Quote: ... Marimastat ((2S,3R)-N-[(1S)-2,2-dimethyl-1-(methylcarbamoyl)propyl]-N’,2-dihydroxy-3-(2-methylpropyl)butanediamide), ɑ2-antiplasmin (SRP6313-100) and recombinant plasminogen activator inhibitor 1 (PAI-1, 528208) were purchased from Sigma-Aldrich (Saint-Louis, MI). Sodium alginate (SA ...
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bioRxiv - Microbiology 2022Quote: ... Expression of recombinant antigens in induced and un-induced controls was assessed by immunoblot using a monoclonal anti-poly- histidine antibody (Millipore-Sigma, diluted 1:2000) after SDS-PAGE ...
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bioRxiv - Developmental Biology 2024Quote: ... EBs and CBs were cultured in the presence of either 3mg/l recombinant SPARC (S5174-25UG, Sigma-Aldrich; 942-SP-050, R&D Systems), various amounts of anti-SPARC antibodies (Santa Cruz ...
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bioRxiv - Cell Biology 2024Quote: ... Supernatants were used for purification of recombinant proteins by 2 h incubation with GST-agarose beads at 4 °C according to manufacturer’s protocol (Sigma Aldrich, St. Louis, USA, G4510). SDS-PAGE electrophoresis was used to check level of expression and purity of purification.
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bioRxiv - Immunology 2023Quote: ... 21 and 42 with 50 μg of each of five recombinant LMWs in alum mixed with 200 ng/mouse pertussis toxin (Sigma-Aldrich, #70323-44-3). ELISA was performed to measure serum responses to antigen (see methods below ...
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bioRxiv - Cell Biology 2024Quote: One rabbit was immunized with 500 μg of recombinant arpin by subcutaneous injection of an emulsified 1:1 mixture of protein and TiterMax Gold (Sigma Aldrich; St. Louis, MO). Three more immunizations were performed 14 ...
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bioRxiv - Cancer Biology 2024Quote: ... The custom recombinant antibody-like MAR binding reagent (anti-MAR) was generated and purified in-house (now available from Millipore Sigma, MABE1076; RRID: AB_2665469) [13] ...
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bioRxiv - Microbiology 2020Quote: ... bacteria were incubated with 0.5 µg/mL of chicken anti-human C1qA (Sigma-Aldrich), followed by incubation with PE-conjugated donkey F(ab’)2 anti-chicken (Jackson ImmunoResearch) ...
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bioRxiv - Immunology 2021Quote: ... 0.4µg pCMV-VSV-G and 1.6µg of a lentiviral vector (for human shSUV39H1, Sigma, TRCN0000157251 ...
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bioRxiv - Developmental Biology 2021Quote: ... were soaked overnight with human SDF-1 3.3 μg ml−1 (Sigma-Aldrich, S1577) at 4 °C ...
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bioRxiv - Genomics 2021Quote: ... Human HEK293T embryonic kidney cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM, Sigma) supplemented with 10% FBS ...
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bioRxiv - Microbiology 2021Quote: Human neutrophils were purified from fresh peripheral blood by Histopaque®-1119 (Millipore Sigma) and subsequent discontinuous Percoll® gradient centrifugation (102) ...
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bioRxiv - Neuroscience 2020Quote: ... sections were incubated with primary antibodies (mouse anti-human synuclein, Sigma S5566 (clone SYN211) for human alpha-synuclein ...
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bioRxiv - Immunology 2022Quote: Human monocytes from 3 donors were culture in 8-well chamber slides (Millipore-Sigma) in SFM ...
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bioRxiv - Immunology 2022Quote: Human monocytes from 3 donors were culture in 8-well chamber slides (Millipore-Sigma) in SFM ...
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bioRxiv - Immunology 2022Quote: Human monocytes from 3 donors were cultured in 8-well chamber slides (Millipore-Sigma) in SFM ...
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bioRxiv - Immunology 2022Quote: Human monocytes from 3 donors were cultured in 8-well chamber slides (Millipore-Sigma) in SFM ...
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bioRxiv - Biochemistry 2020Quote: ... full length human PCNA (accession no. NM182649) was cloned into pETDuet-1 MCS1 (Novagen) Amp+ to obtain 6× His N-terminally tagged protein ...
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bioRxiv - Immunology 2020Quote: ... pMCPECs media supplemented with 5μg/ml human insulin (Sigma Aldrich, St Louis, MO, USA), 10ng/ml hEGF (Peprotech ...
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bioRxiv - Immunology 2020Quote: ... and DENV-specific antibody binding quantified using anti-human IgG HRP (Sigma-Aldrich, SAB3701362). Secondary antibody binding was quantified using the TMB Microwell Peroxidase Substrate System (KPL ...
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bioRxiv - Pathology 2021Quote: Cell culture inserts were coated with human placental type IV collagen (Sigma-Aldrich, C7521). CRC expanded cells were seeded at 2.5 × 105 cells on 12-mm Snapwells (Corning Costar ...
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bioRxiv - Developmental Biology 2021Quote: ... they underwent intraperitoneal injection of 20 IU human chorionic gonadotropin (9002-61-3, Sigma) (27) ...
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bioRxiv - Developmental Biology 2021Quote: ... and seeding on 16.7 µg/ml human plasma fibronectin-coated plates (Merck Millipore, FC010). After 48h ...
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bioRxiv - Immunology 2021Quote: ... human endometrial cells were incubated for 4 hours with monensin and Brefeldin A (Sigma) in the presence or absence of 100 ng/ml LPS (Sigma) ...
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bioRxiv - Immunology 2021Quote: ... and DENV-specific antibody binding quantified using anti-human IgG HRP (Sigma-Aldrich, SAB3701362). Secondary antibody binding was quantified using the TMB Microwell Peroxidase Substrate System (KPL ...
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bioRxiv - Microbiology 2021Quote: ... and differentiated for one week in RPMI containing 10% human AB serum (H4522, Sigma), GlutaMAX ...
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bioRxiv - Bioengineering 2020Quote: ... anti-human mitochondria antibody Alexa Fluor® 488 conjugate (MAB1273A4, 1:100, EMD Millipore), and DAPI.
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The E3 ubiquitin-protein ligase MDM2 is a novel interactor of the von Hippel-Lindau tumor suppressorbioRxiv - Biochemistry 2020Quote: Co-immunoprecipitation experiments were performed using human kidney HEK293T cells provided by (Sigma-Aldrich). Cells were plated in a 1 ml well microplate and transfected at 70-80% confluence using Lipofectamine 2000 following the manufacturer protocols (Invitrogen) ...
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bioRxiv - Neuroscience 2021Quote: ... MaxiSorp plates (Thermo) were coated with 100µl of goat anti-human Fc antibody (Sigma) diluted 1:500 in PBS overnight at 4°C ...
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bioRxiv - Neuroscience 2021Quote: ... Plates were washed before detection and goat anti-human Fc-HRP conjugated antibody (Sigma) (1:5000 dilution in PBS ...
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bioRxiv - Cell Biology 2021Quote: ... were coated with 5 µg/mL human plasma fibronectin purified protein (EMD Millipore Corporation). Cells were seeded at a density of 315,000 cells per insert in 250 µL HITES media ...
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bioRxiv - Biochemistry 2020Quote: ... then resuspended in 1:500 anti-human IgG antibody conjugated to Cy3 (Sigma #C2571). Fluorescence staining was measured by a BD Fortessa flow cytometer.
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bioRxiv - Microbiology 2020Quote: ... 5 μg human insulin mL-1 and 5×10-7 M hydrocortisone hemisuccinate (Sigma). Cells were seeded and expanded for two weeks and subsequently differentiated for another two weeks in the presence of 1.8% DMSO61 (dHepaRG).
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bioRxiv - Microbiology 2020Quote: ... 5 μg human insulin mL−1 and 5×10−7 M hydrocortisone hemisuccinate (Sigma). As a control we generated HepaRG cells expressing an inactive HBx null mutant (HepaRG-HBxSTOP ...
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bioRxiv - Neuroscience 2020Quote: ... The human neuroblastoma cell line SH-SY5Y was obtained from Sigma (Cat no. 94030304) at passage number 5 and used for generation of all other CRISPR-Cas9 edited cell lines ...
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bioRxiv - Cancer Biology 2021Quote: ... Cells were treated with 500 ng/ml human anti-Fas (Millipore Sigma, Clone ZB4) with and without 1000 ng/ml of TRAIL ...
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bioRxiv - Cell Biology 2021Quote: ... dishes and flasks were coated in 10 μg/ml human plasma fibronectin (FN) (Millipore) overnight at 4°C ...
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bioRxiv - Cell Biology 2021Quote: ... the human cTnC gene (TNNC1) within the pET-21a(+) vector was ordered from Novagen and the Phusion site directed mutagenesis kit (Thermo ...
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bioRxiv - Neuroscience 2022Quote: ... The following synaptic antibodies were used: Rabbit anti-human SYNAPSIN1 (1:1000, Millipore, AB1543), Chicken anti-human MAP2 (1:1000 ...
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bioRxiv - Cancer Biology 2022Quote: The human tongue OSCC lines (H357, SCC4 and SCC9) were obtained from Sigma Aldrich, sourced from European collection of authenticated cell culture ...
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bioRxiv - Cancer Biology 2022Quote: ... 50,000 human ECs (RBPJ knock-out or shRNA for CXCL2 (pLKO.1; Sigma Aldrich) and respective controls ...
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bioRxiv - Microbiology 2022Quote: ... Plates with Towne virus were stained with mouse anti-human CMV IE1 (MAB810, Millipore) then goat anti-mouse IgG-AF488 (Millipore) ...