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Citations for Millipore Sigma :
2701 - 2750 of 4279 citations for Recombinant Human ABHD15 His tagged since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2024Quote: ... NPE was supplemented with 50 μg/mL recombinant GST-p27Kip (“CDK-i”) or 50 μM PHA-767491 (Sigma-Aldrich PZ0178, “DDK-i”) and pre-incubated at room temperature for 15 mins prior to addition to licensing reaction ...
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bioRxiv - Biophysics 2024Quote: ... Some recombinant proteins were run on a size-exclusion column (LP BIOrad system; HiPrepTM 26/60 Sephacryl® S-300 HR, Sigma-Aldrich) to improve purity (see supplemental materials for final proteins).
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bioRxiv - Developmental Biology 2024Quote: ... E14 embryonic mouse stem cells were expanded in 2iLIF media and transferred to StemFlex supplemented with 1000 U ESGRO recombinant mouse leukemia inhibitory factor (mLIF; Sigma-Aldrich #ESG1107) before generation of organoids using the same protocol as for human ...
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bioRxiv - Genetics 2021Quote: ... Anti-His (SCBT, #sc-8036; used in 1:2000), anti-GST (SCBT, #459; used in 1:5000) and anti-Flag (Sigma-Aldrich, #F7425;used in 1:2000). Peroxidase conjugated anti-mouse (Jackson Immunoresearch ...
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bioRxiv - Cell Biology 2020Quote: A2780 human ovarian cancer cells were maintained in RPMI-1640 medium (Sigma-Aldrich) supplemented with 10% (v/v ...
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bioRxiv - Developmental Biology 2021Quote: ... mouse anti-human α-smooth muscle actin (Cy3 conjugated, cat. #C6198, Sigma Aldrich, 1:500 ...
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bioRxiv - Microbiology 2021Quote: ... Cells were then suspended in veronal buffer and human serum (Sigma-Aldrich, H4522) (or heat-inactivated for 30min at 56°C) ...
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bioRxiv - Molecular Biology 2021Quote: The gene of human GAPDH was cloned into pET-28a(+) expression vector (Novagen) with an N-terminal His-tag followed by the Tobacco Etch Virus (TEV ...
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bioRxiv - Biochemistry 2022Quote: ... or −3 human cDNA into a p3XFLAG-CMV-14 expression vector (Sigma, E7908). Then 3XFLAG-PRLs were cloned into pLenti-CMV-puro (Addgene 17452 ...
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bioRxiv - Cell Biology 2022Quote: ... dried and incubated with 10 μg/mL of IgG from human serum (Sigma) in PBS for 1 hour at RT ...
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bioRxiv - Neuroscience 2021Quote: ... were injected with 400 IU human chorionic gonadotropin (hCG; Sigma-Aldrich CG10; RRID:SCR_018232).
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bioRxiv - Molecular Biology 2021Quote: ... bleomycin and human activating FAS (anti-FAS) antibody were purchased from Millipore Sigma. PGE2 ...
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bioRxiv - Immunology 2022Quote: ... media was replenished with RPMI containing 5% type AB human serum (Sigma-Aldrich). For single-round experiments with VSV-G-pseudotyped viruses ...
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bioRxiv - Cell Biology 2020Quote: ... in acetic acid (0.02 N) or human plasma fibronectin (5 µg/mL; Millipore) in PBS overnight at 4°C and rinsed with imaging medium to remove the coating solution ...
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bioRxiv - Cell Biology 2020Quote: The human ovarian cancer cell lines COV318 (purchased from ECACC, via Sigma-Aldrich) and SKOV3 (courtesy of Dr Florence Delie ...
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bioRxiv - Immunology 2021Quote: ... Total IgA purified from human serum was used as a standard (Sigma-Aldrich). To generate the IgA standard curve anti-human IgA capture antibodies ...
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bioRxiv - Immunology 2021Quote: ... and then overlaid with complete DMEM containing 10 μg/mL human IgG (Sigma). 48 hours post infection ...
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bioRxiv - Microbiology 2021Quote: ... Primary human monocytes were treated with 50 ng/ml M-CSF (Sigma-Aldrich) in complete RPMI for a week before infection ...
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bioRxiv - Cancer Biology 2021Quote: MISSION shRNA targeting mouse or human PSME4 or RFP were obtained from Sigma (TRCN0000176569 ...
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bioRxiv - Bioengineering 2021Quote: ... and human β2m were purchased from IDT and cloned into pET3a vectors (Novagen). H2-Db and H2-Kb genes were engineered with a C-terminal cysteine by site-directed mutagenesis (NEB) ...
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bioRxiv - Bioengineering 2021Quote: Human serum albumin (> 97% lyophilized) was purchased from Sigma Aldrich (Oakville, ON, Canada). Glutaraldehyde (25% aq ...
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bioRxiv - Bioengineering 2021Quote: ... Serum pramlintide concentrations were quantified using a human amylin ELISA kit (Millipore Sigma). Serum lispro concentrations were quantified using Northern Lights Mercodia Lispro NL-ELISA ...
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bioRxiv - Bioengineering 2021Quote: ... 100 ng/mL Follicle Stimulating Hormone from human pituitary (FSH, Millipore Sigma, F4021), 10 ng/mL LH ...
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bioRxiv - Biochemistry 2021Quote: FFPE human tonsils were deparaffinized in xylene substitute (Sigma-Aldrich, St. Louis, MO), rehydrated in a graded ethanol series and stained for 10 minutes with Atto 465-carboxcylic acid (8 uM ...
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bioRxiv - Biophysics 2021Quote: ... lyophilized rabbit muscle aldolase and lyophilized human hemoglobin were purchased from Sigma Aldrich. The catalase was diluted to a final concentration of 2.3 mg ml-1 in the buffer of PBS (pH 6.5) ...
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bioRxiv - Neuroscience 2021Quote: ... were injected with 400 IU human chorionic gonadotropin (hCG; Sigma-Aldrich CG10; RRID:SCR_018232). The following day ...
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bioRxiv - Cancer Biology 2021Quote: siRNAs targeting human SUV39H1/H2 and negative control siRNAs72 were from Sigma-Aldrich. Transfections were performed using Lipofectamine RNAiMax (Thermo Fisher ...
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bioRxiv - Biophysics 2021Quote: ... pre-coated with 30 μg/ml of fibronectin from human plasma (Sigma-Aldrich) and nanodiamonds (100 nm ...
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bioRxiv - Neuroscience 2020Quote: ... Plates were incubated with anti-human Fab specific-HRP conjugated secondary antibody (Sigma) for 1 h at RT ...
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bioRxiv - Bioengineering 2021Quote: ... a standard curve was obtained by diluting human Hgb powder (Sigma-Aldrich, USA) in human plasma (Cambridge Bioscience ...
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bioRxiv - Microbiology 2021Quote: Human umbilical VECs (Cell Application 200-05n) were cultured on fibronectin (Sigma 10838039001)-coated glass slides or polyacrylamide (PA ...
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bioRxiv - Immunology 2021Quote: ... cells were resuspended in PBS with 2% Human Heat Inactivated AB Serum (Sigma) and 0.1 M EDTA pH 8.0 (Invitrogen ...
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bioRxiv - Cancer Biology 2020Quote: Cells were treated with the indicated concentrations of DLK1 human (Sigma Cat# SRP8006) or Recombinant Human DLK-1 protein (Abcam Cat# ab151926) ...
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bioRxiv - Cell Biology 2021Quote: ... 350 μl of 1:25 diluted 0.1% human serum derived fibronectin (Sigma-Aldrich) was added per stamp and incubated at room temperature for 1 hour ...
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bioRxiv - Cell Biology 2021Quote: ... human β2 adrenergic receptor (β2ADR) with isoproterenol (Iso; Sigma-Aldrich I5627, in water) Epinephrine (Sigma-Aldrich E4642 ...
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bioRxiv - Cancer Biology 2021Quote: Organoids and human tissue were fixed with 3.7% paraformaldehyde (PFA, Sigma-Aldrich, # 4441244), dehydrated ...
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bioRxiv - Molecular Biology 2022Quote: ... penicillin-streptomycin (Thermo 15140122) and 10 ug/mL human transferrin (Sigma-Aldrich T8158). HCA-7 cells were maintained in a 1:1 mix of DMEM and F12 (Thermo 11320033 ...
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bioRxiv - Immunology 2022Quote: ... Bound antibodies were subsequently detected with AP-conjugated goat anti-human IgA (Sigma) or goat anti-human IgG (Southern Biotech).
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bioRxiv - Immunology 2022Quote: ... The human cytokine/chemokine magnetic bead kit (38-plex) (HCYTOMAG-60K-38X, Millipore) and a custom-made panel (36-plex ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... in which the plate was coated with anti–human Fc (Sigma, Catalog # FAB3700259), and after overnight incubation at 4°C ...
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bioRxiv - Microbiology 2022Quote: Human Mel Juso cells were maintained in Dulbecco’s Modified Eagle Medium (DMEM, Sigma) containing 10% heat-inactivated fetal calf serum (FCS ...
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bioRxiv - Cell Biology 2022Quote: ... A standard curve of human genomic DNA from buffy coat (Sigma-Aldrich #11691112001) along with positive and negative controls were included on each of plates to assess plate-to-plate variability and ensure that values fell within measurement range ...
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bioRxiv - Microbiology 2022Quote: ... Coverslips were blocked and permeablized in staining buffer [1% human serum (Sigma,4552) heat-inactivated at 56°C for 1h ...
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bioRxiv - Immunology 2022Quote: Human peripheral blood mononuclear cells were collected using Ficoll Histopaque-1077 (Sigma-Aldrich) and cultured in Opti-MEM Reduced Serum Media (Invitrogen) ...
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bioRxiv - Cancer Biology 2022Quote: The human tongue OSCC lines (H357 and SCC4) were obtained from Sigma Aldrich, sourced from European collection of authenticated cell culture ...
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bioRxiv - Molecular Biology 2020Quote: ... in the presence of human VEGF (10-20 ng/mL, Sigma Aldrich, MO). Following 4 hours of incubation at 37 °C ...
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bioRxiv - Physiology 2020Quote: ... Insulin release from islets was measured with Human Insulin ELISA kit (Millipore Sigma). See Table S1 for donor information.
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bioRxiv - Immunology 2020Quote: ... 25 μL/well of alkaline phosphatase-conjugated goat anti-human IgG (Sigma-Aldrich) was added for 1h at 37°C without CO2 ...
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bioRxiv - Immunology 2020Quote: ... 25 μL/well of alkaline phosphatase-conjugated goat anti-human IgG (Sigma-Aldrich) and IgA (Jackson Immuno Research ...
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bioRxiv - Immunology 2020Quote: Human THP-1 monocytic cells (ATCC) were cultured in RPMI 1640 (Sigma-Aldrich) medium containing 10% heat-activated fetal calf serum (FCS) ...