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Citations for Millipore Sigma :
2801 - 2850 of 8516 citations for V Bottom Microplate 96 well since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2019Quote: ... were dissolved in ethanol (96%; Sigma-Aldrich) at 100 µM and then diluted in medium at the final concentration (100 nM) ...
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bioRxiv - Neuroscience 2023Quote: ... striatal neurons were plated at a density of 50K per well onto 24 well plates or 20K per well onto 8 well chamber slides coated with poly-L-lysine (Sigma Aldrich, St. Louis, USA) (100 µg/ml) ...
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bioRxiv - Immunology 2020Quote: ... 384-well plates (Nunc MaxiSorp 384 well plates; Sigma Aldrich) were coated with 3μg/mL of streptavidin diluted in PBS and incubated at RT overnight ...
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bioRxiv - Immunology 2020Quote: ... 384-well plates (Nunc MaxiSorp 384 well plates; Sigma Aldrich) were coated with 3μg/mL of streptavidin diluted in PBS and incubated at RT overnight ...
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bioRxiv - Cell Biology 2020Quote: ... and then lysed on ice in lysis buffer (10 mM Tris-HCl pH 8.0; 10 mM NaCl; 0.5% (v/v) NP-40 substitute (Sigma); 1% (v/v ...
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bioRxiv - Immunology 2021Quote: ... and 10% (v/v) fetal calve serum (FCS)) with 1.6 mg collagenase (Sigma-Aldrich, St. Louis, MO, USA) and processed twice on a GentleMacs using the lung program (Miltenyi Biotec ...
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bioRxiv - Immunology 2021Quote: ... Membranes were washed twice for 5 min in PBS plus 0.1% (v/v) Triton X-100 (Sigma-Aldrich) before being blocked with a 1% (w/v ...
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bioRxiv - Developmental Biology 2021Quote: ... and two 5 min washes in 100% methanol and clearing in 1:1 v/v methanol/BA:BB (2:1 benzyl alcohol:benzyl benzoate; Sigma), and two washes in 100% BA:BB ...
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bioRxiv - Cell Biology 2020Quote: ... glass-bottomed dishes were first cleaned and activated with 1% v/v (3-Aminopropyl) triethoxysilane (APTES, Sigma-Aldrich) in ethanol ...
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bioRxiv - Neuroscience 2021Quote: ... tissue fragments were washed once with 10% (v/v) heat-inactivated fetal bovine serum (FBS, F2442 Sigma Aldrich) in Neurobasal Plus medium (A3582901 ...
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bioRxiv - Molecular Biology 2021Quote: ... supplemented with pyruvate and glutamine (Difco) and 10% (v/v) heat-inactivated foetal calf serum (FCS) (Sigma-Aldrich) (D10) ...
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bioRxiv - Biochemistry 2019Quote: ... the probes were transferred into a 0.1% (v/v) solution of 3-aminopropyltrimethoxysilane (APTES) (Sigma, St. Louis, MO) in toluene for 2 h ...
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bioRxiv - Microbiology 2019Quote: ... 0.03 M KCl and 0.01 M phosphate buffer plus Tween 20 (0.05% v/v)) before incubation with HRP-conjugated secondary antibodies (Sigma). The resolved proteins on the membrane blots were detected using the Novex ECL HRP Chemiluminescent substrate (Invitrogen ...
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bioRxiv - Plant Biology 2019Quote: ... with 1% (w/v) sucrose, 0.5 g/L of MES pH 5.8 (Duchefa, Holland) and 0.8% (w/v) agar (Sigma, USA). Seeds in the plates were stratified in the dark for three days at 4°C and then transferred to a growth chamber at 22-23°C temperature and 16 h light-8 h darkness photoperiod ...
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bioRxiv - Developmental Biology 2019Quote: ... then resuspended in 2ml papain solution [0.75% v/v of papain (25mg/ml, 17U/mg protein, Sigma-Aldrich), 40μg/ml DNase I type IV ...
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bioRxiv - Cell Biology 2019Quote: ... Jackson) at 1:200 (v/v) for 30 min at room temperature followed by DAPI staining (Sigma-Aldrich) at 1:3000 (v/v ...
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bioRxiv - Genetics 2021Quote: ... Complexes were diluted to 2.5uM in a protein storage buffer composed of 50% (v/v) glycerol (Sigma G5516), 100 mM NaCl (Fisher Scientific S271-3) ...
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bioRxiv - Bioengineering 2020Quote: ... The coverslips were then submerged in a solution containing 2% (v/v) aminopropyltriethoxysilane (APTES) (Millipore Sigma, Burlington, USA) with 90% (v/v ...
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bioRxiv - Bioengineering 2020Quote: ... All aqueous master mixtures contained purified water with 21.4% v/v PEG-DA (Sigma-Aldrich, average Mn 700) and 5% v/v YVO4:Eu (50 mg/mL) ...
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bioRxiv - Biochemistry 2020Quote: ... and 15 mM phosphoenolpyruvate in presence of a 4% v/v pyruvate kinase/lactate dehydrogenase enzyme mix (Sigma). Reactions were initiated by the addition of ATP to a final concentration of 25 μM - 6.4 mM ...
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Plant pathogens convergently evolved to counteract redundant nodes of an NLR immune receptor networkbioRxiv - Plant Biology 2021Quote: ... Beads were washed five times with immunoprecipitation wash buffer [GTEN extraction buffer with 0.3% (v/v) IGEPAL (SIGMA)] and re-suspended in 70μl SDS loading dye ...
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bioRxiv - Microbiology 2021Quote: ... Slides were blocked with bovine serum albumin for 1 hour at room temperature (Sigma-Aldrich, 1% v/v).
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bioRxiv - Cancer Biology 2020Quote: EVs and lysate samples resuspended in denaturation buffer were mixed with freshly β-mercaptoethanol (5% (v/v)) (Sigma) and heated for 10 minutes at 95 °C ...
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bioRxiv - Biophysics 2020Quote: ... a mica surface was treated for 5 min with 0.01% (v/v) (3-aminopropyl)triethoxysilane (APTES) (Sigma-Aldrich). HS-AFM observations of the 50S subunits from Pyrococcus furiosus or pre-assembled hybrid 50S subunits were performed as follows ...
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bioRxiv - Cancer Biology 2021Quote: ... followed analysis of cell surface annexin-V and cell permeability using the Annexin-V-FLUOS staining kit (Sigma). Flow cytometry was performed on live cells using a BD FACSCalibur cytometer at the McGill Life Sciences Complex Flow Cytometry Facility.
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bioRxiv - Bioengineering 2022Quote: ... The PEG pre-polymer phase comprised 27.5% w/v 5kDa 4-arm PEG acrylate (Advanced BioChemicals) with 4% w/v lithium phenyl-2,4,6-trimethylbenzoylphosphinate (LAP, Sigma) in phosphate buffered saline (PBS ...
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bioRxiv - Cell Biology 2022Quote: ... The pellet was resuspended in a 15 mL reaction tube with 1.5 mL 20% acetic acid pH 3.5 (v/v, Sigma), 200μL 10% SDS (Sigma) ...
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bioRxiv - Plant Biology 2021Quote: Arabidopsis seeds were surface sterilized as described above and plated on ½-strength MS media containing 0.5% (w/v) MES (pH 5.7) and 0.8% (w/v) agar containing ABA (Sigma-Aldrich), NaCl ...
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bioRxiv - Microbiology 2020Quote: Air-dried thin blood films of asexual parasites were fixed with 4% paraformaldehyde containing 0.0075% glutaraldehyde for 15 min and permeabilized in 0.1% (v/v) Triton X-100 (Sigma) for 10 min 40 ...
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bioRxiv - Plant Biology 2021Quote: ... containing 0.5% (w/v) sucrose and solidified with 1% (w/v) Agar Type M (Sigma-Aldrich, Steinheim, Germany), on square polypropylene petri dishes (120 mm x 120 mm ...
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bioRxiv - Cell Biology 2021Quote: ... Approximately 10 ml of exponential cultures (OD600 = 0.5-1) were fixed with 4% (v/v) formaldehyde (Sigma-Aldrich) during 15 min at room temperature or 37 °C ...
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bioRxiv - Neuroscience 2020Quote: ... Anesthesia was induced with 4% v/v isoflurane (Isoflo; Schering-Plough) in O2 and maintained with urethane (1.3g/kg, i.p.; ethyl carbamate, Sigma), and supplemental doses of ketamine (30 mg/kg ...
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bioRxiv - Biochemistry 2020Quote: ... Foam formation was avoided by the addition of 3 % (v/v) antifoam 289 (Sigma-Aldrich; St. Louis, MO). Samples were withdrawn throughout the fermentation process with the purpose of evaluating the biomass and recombinant protein expression.
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bioRxiv - Neuroscience 2019Quote: ... After 3 times washing with PBS-T [PBS with 0.5% (v/v) Triton X-100 (Sigma Aldrich, # X100)] fixed brains were incubated with primary antibody containing 10% Normal Donkey Serum in 0.4% PBS-T overnight at 4°C ...
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bioRxiv - Bioengineering 2020Quote: ... Cell culture media were prepared by adding 10% (v/v) of Foetal Bovine Serum (FBS, Sigma-Aldrich, UK), 2 mM L-glutamine (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2019Quote: ... 1% (v/v) Triton X-100 and 0.5% sodium deoxycholate) supplemented with protease inhibitor coktail (Sigma-Aldrich, #05892953001), 1 mM Na3VO4 and 25 mM sodium fluoride and centrifuged at 16,000xg for 15 min ...
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bioRxiv - Cell Biology 2020Quote: ... Ovalbumin (OVA, grade V) and bovine serum albumin (BSA, fraction V) were from Sigma-Aldrich (Saint Louis, MO). All oligonucleotides used were customized and ordered from Integrated DNA Technologies (Coralville ...
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bioRxiv - Bioengineering 2020Quote: ... Non-specific binding sites were blocked by incubation in 10% (v/v) normal donkey serum (D9663; Sigma-Aldrich) and 1% (v/v ...
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bioRxiv - Biophysics 2020Quote: ... samples were incubated with 2 % (v/v) primary anti-tubulin antibody (clone B-5-1-2, Sigma-Aldrich) in blocking buffer for 60 min at room temperature ...
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bioRxiv - Genomics 2020Quote: ... 20 µl protein extracts diluted 1:1 (v/v) with ice-cold 2x Laemmli sample buffer (Sigma-Aldrich) were separated by 12% SDS-PAGE ...
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bioRxiv - Cancer Biology 2021Quote: ... All cell lines were cultured in RPMI1640 media supplemented with 10% (v/v) fetal bovine serum (FBS, Sigma), 2 mM glutaMAX (Gibco ...
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bioRxiv - Biochemistry 2019Quote: ... supplemented with 5% (v/v) fetal bovine serum (Life Technology) and 100 g/ml penicillin/streptomycin (Sigma-Aldrich) at 37°C with 5% CO2 ...
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bioRxiv - Immunology 2019Quote: ... Total cell lipids were extracted using 7:11:01 (v/v) chloroform:isopropanol:IGEPAL® CA-630 (#I8896, Sigma-Aldrich), dried at 50°C and re-suspended in 120µl cholesterol assay buffer (#MAK043 ...
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bioRxiv - Systems Biology 2019Quote: ... Chloroform-methanol (1:1 v/v; 400 µl) and BF3–methanol (10%; 125 µl) (Sigma-Aldrich, Dorset, UK) were added to the dried organic phase and incubated at 80 °C for 90 min ...
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bioRxiv - Genetics 2020Quote: ... and 50 mM MgCl2) and stained in ALP buffer containing 2% (v/v) NBT/BCIP (Sigma, cat# 11681451001). After a brief wash in deionised water ...
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bioRxiv - Cell Biology 2020Quote: ... Both the CS and cantilevers were washed with acetone and functionalized using 2% (v/v) 3-aminopropyltriethoxysilane (Sigma) dissolved in acetone ...
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bioRxiv - Biochemistry 2021Quote: ... Formic acid was added at 0.1% v/v in a final volume of 50 μl and samples were filtered (0.22 μm, Millipore) and 15-20 μl analyzed in duplicate by LC-MS ...
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bioRxiv - Genetics 2021Quote: ... Lysed worm powder was thawed on ice in a final volume of 3.75 ml lysis buffer including 0.5 % v/v NP-40 (Sigma), 0.4 U/μL RNasin (Promega) ...
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bioRxiv - Cancer Biology 2021Quote: ... Human cancer cell lines were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM) supplemented with 10% v/v foetal bovine serum (FBS, Sigma), penicillin (100 units/mL ...
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bioRxiv - Microbiology 2021Quote: ... Librarybearing cells were plated onto LB agar plates (15 g/L) containing 1% (v/v) emulsified tributyrin (Sigma) as the indicator substrate and the appropriate antibiotic (pFLD ...