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Citations for Millipore Sigma :
2801 - 2850 of 10000+ citations for Recombinant Human CD22 protein His tagged APC labeled since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... then concentrated using 15 ml Amicon 100 kDa centrifuge filter columns and purified by nickel column affinity chromatography (Novagen His-Bind Resin) as per the manufacturer’s instructions.
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bioRxiv - Plant Biology 2024Quote: The open reading frame of WsGT4 and WsGT6 was cloned into the NheI/HindIII and NdeI/BamHI sites of pET28a bacterial expression vector in-frame with the C-terminal 6X-His-tag (Novagen, http://www.emdbiosciences.com) resulting in pET28a::WsGT4 and pET28a::WsGT6 respectively (Figure S1 A&B) ...
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bioRxiv - Plant Biology 2024Quote: The open reading frame of CfADH1 and CfAKR2b was cloned into the NdeI/EcoRI sites of pET28a bacterial expression vector in-frame with the C-terminal 6X-His-tag (Novagen, http://www.emdbiosciences.com) resulting in pET28a::CfADH1 and pET28a::CfAKR2b ...
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bioRxiv - Cell Biology 2019Quote: Total cellular RNA was labeled for 3 hours with EU (Thermo-Fisher, E10345) or BrU (Sigma, 850187). EU-RNA was detected using the Click-iT RNA Imaging Kit (Thermo Fisher ...
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bioRxiv - Molecular Biology 2020Quote: EVs were labeled with the PKH26 Red Fluorescent Cell Linker Kit for General Cell Membranes (Sigma-Aldrich). Briefly ...
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bioRxiv - Molecular Biology 2021Quote: ... Chitin in the ASG was labeled using FITC conjugated wheat germ agglutinin (FITC-WGA, Sigma-Aldrich, USA).
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bioRxiv - Molecular Biology 2021Quote: A CU RNA hairpin (5’-AAAAGAAAAGACGCGUAGUUUUCUACGCG- 3’) labeled with Cyanine 5.5 at the 5’-end (Millipore Sigma) was annealed in 20 mM HEPES ...
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bioRxiv - Biochemistry 2019Quote: ... Labeled viruses were further purified by ultracentrifugation at 40,000 rpm over a 6%-18% Optiprep (Sigma Aldrich) gradient for 1 hour.
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bioRxiv - Microbiology 2019Quote: ... Hybridizations and detection of the DIG-labeled DNA probe were performed according to manufacturer’s suggestions (Sigma-Aldrich). Blots were visualized using a Bio-Rad Chemidoc system.
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bioRxiv - Genetics 2019Quote: ... RNA digoxigenin-labeled probe was synthesized by in vitro transcription using Dig RNA labeling kit (Sigma-Aldrich). For in situ hybridization ...
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bioRxiv - Biophysics 2020Quote: ... 4 ng/µl of the multiple-labeled goat anti-mouse IgG secondary antibodies (Sigma-Aldrich, SAB3701063-1) for the β-tubulin samples and 4 ng/µl of the multiple-labeled goat anti-rabbit IgG secondary antibody DOL 8.3 (Invitrogen ...
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bioRxiv - Molecular Biology 2021Quote: ... PAN specific oligonucleotide probes were labeled with a DIG-Oligonucleotide tailing kit (Sigma Roche 2nd generation 03353583910) and mixed at the 1:1:1 ratio ...
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bioRxiv - Microbiology 2020Quote: ... Membranes were labeled in 5% BSA/TBST with either anti-HA peroxidase conjugated antibodies (Sigma, 1:200) or rabbit TgALD1 antibody (1:200 ...
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bioRxiv - Neuroscience 2021Quote: DIG-labeled DNA probes were synthesized using Roche PCR DIG Probe Synthesis Kit (Millipore Sigma ca#11636090910). Probes were designed to target either the LexA coding sequence (Probe 1 ...
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bioRxiv - Cell Biology 2021Quote: ... MTs were labeled with rat monoclonal antibody YL 1/2 directed against tyrosinated -tubulin (1:1000, Millipore), or rabbit polyclonal antibody against detyrosinated -tubulin (1:1000 ...
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bioRxiv - Genetics 2022Quote: ... The membrane was hybridized with 5 pmol/ml of a 5’ DIG-labeled (AGAGGG)10 probe (Sigma) in DIG Easy Hybridization Solution (Sigma ...
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bioRxiv - Biophysics 2022Quote: ... rhodamine-B-labeled dextran with a molar mass of 10 kg/mol (RB-Dex10k; Sigma–Aldrich Japan), and rhodamine-B-labeled PEG with a molar mass of 5 kg/mol (RB-PEG5k ...
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bioRxiv - Cell Biology 2022Quote: The paracellular permeability was determined by using fluorescein isothiocyanate labeled 4kD-dextran (Sigma-Aldrich, St. Louis, USA). 18 hours after addition of 0.2 mg FITC-dextran in 200 µL medium to the apical side of the ALI cultures ...
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bioRxiv - Microbiology 2020Quote: ... the cultures were labeled with 1mM Sodium bicarbonate-13C and 1mM Ammonium-15N chloride (Sigma-Aldrich, USA) for 18-24 hours ...
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bioRxiv - Immunology 2020Quote: ... The isolated B cells from WT and MIM−/− mice were labeled with 1μM CFSE (21888, Sigma-Aldrich) or left unlabeled ...
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bioRxiv - Biochemistry 2020Quote: ... 13C/15N labeled RNA was buffer exchanged using centrifugal concentration (3 kDa molecular weight cutoff, EMD Millipore) into NMR buffer (15 mM NaH2PO4/Na2HPO4 ...
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bioRxiv - Developmental Biology 2020Quote: ... we synthesized fluorescein-labeled RNA probes with the Fluorescein RNA labeling mix (Sigma-Aldrich; Cat. No. 11685619910) and hybridized together with a DIG-labeled probe of another gene ...
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bioRxiv - Developmental Biology 2020Quote: ... we synthesized DIG-labeled RNA probes with the DIG RNA labeling mix (Sigma-Aldrich; Cat. No. 11277073910) and T7 RNA polymerase (Promega ...
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bioRxiv - Physiology 2020Quote: ... a secondary anti-rabbit or anti-mouse antibody labeled with Horse Radish Peroxidase (HRP) (NA934VS, Millipore Sigma) was added at a 1/3000 dilution in 4% milk in TBST for 30 min at RT ...
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bioRxiv - Developmental Biology 2020Quote: ... In vitro transcription of digoxigenin-labeled probes was performed using the DIG RNA Labeling Kit (Millipore Sigma) according to manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2020Quote: All Digoxygenin (DIG) labeled probes were generated using the Roche DIG labeling kit (Sigma Cat no 11277073910). Antisense probes were generated using Pme1 (NEB cat no R0560S ...
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bioRxiv - Cell Biology 2021Quote: ... Saturated bacterial cultures of OP50 were mixed with 4mM deuterium labeled PA-d31 or OA-d34 (Sigma) and then seeded onto NGM plates ...
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bioRxiv - Biophysics 2022Quote: ... labeled cell pellets were resuspended in 500 uL of wash buffer supplemented with benzonase nuclease (Sigma Aldrich) at 1:100,000 dilution and EDTA-free protease inhibitors (Sigma Aldrich ...
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bioRxiv - Biophysics 2022Quote: ... and 1,2-Dioleoyl-sn-glycero-3-phosphoethanolamine labeled with Atto 647N (Atto647N-DOPE) purchased from Sigma-Aldrich.
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bioRxiv - Cancer Biology 2024Quote: ... Cells were sequentially pulse-labeled with 50 μM of 5-chloro-2′-deoxyuridine thymidine (CldU) (Sigma-Aldrich) and 250 μM of idoxuridine (IdU ...
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bioRxiv - Molecular Biology 2023Quote: ... Total DNA was labeled with 2 μg/ml of 7-AAD (7-amino-actinomycin D, Sigma-Aldrich) diluted in PBS containing 100 μg/ml of RNAse A (Sigma -Aldrich) ...
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TIPRL1 and its ATM-dependent phosphorylation promote radiotherapy resistance in head and neck cancerbioRxiv - Cancer Biology 2023Quote: ... cells were irradiated with 6 Gy and DNA was labeled with 75 µM Bromodeoxyuridine (BrdU) (Sigma, #B5002) 1h before the indicated time points ...
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bioRxiv - Developmental Biology 2023Quote: ... Labeled embryos were anesthetized with 0.17 mg/ml ethyl 3-aminobenzoate methanesulfonateMS-222 (E10521-50G, Sigma-Aldrich), embedded in 0.7% agarose gel ...
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bioRxiv - Molecular Biology 2023Quote: ... Fluorescently labeled G3BP1 (see Methods) at 7 nM was titrated with different concentrations of polyU (Sigma Aldrich) in 50 mM HEPES ...
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bioRxiv - Bioengineering 2023Quote: Either iPSC or iMSC-derived EVs were labeled with PKH67 (PKH67GL; Sigma-Aldrich, St. Louis, MO, USA). EVs were buffer exchanged with Diluent C using a 300 kDa MWCO Nanosep (OD300C35 ...
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bioRxiv - Genetics 2023Quote: ... 250–500 ng of labeled plasmid and 12.5–25 μg of sonicated salmon sperm DNA (Sigma-Aldrich) were used per slide ...
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bioRxiv - Genetics 2023Quote: ... 250–500 ng of labeled plasmid and 12.5–25 μg of sonicated salmon sperm DNA (Sigma-Aldrich) were applied per slide ...
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bioRxiv - Microbiology 2023Quote: ... the supernatant was isometrically mixed with the internal standards labeled with stable isotopes (Sigma-Aldrich, Shanghai, China) for UHPLC-MS/MS analysis.
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bioRxiv - Cancer Biology 2023Quote: ... cells were labeled with 25 µM 5-Chloro-2’-deoxyuridine (CldU, Sigma-Aldrich, CAS 50-90-8) for 30 min ...
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bioRxiv - Immunology 2023Quote: Mice were orally gavaged (0.6 mg/kg body weight) with 4 kDa dextran labeled with fluorescein (Sigma) and serum was collected in EDTA containing blood collection tubes (BD ...
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bioRxiv - Developmental Biology 2024Quote: ... and/or 20 μg ml-1 AlexaFluor 488-labeled peanut (Arachis hypogaea) agglutinin lectin (PNA, L21409, Sigma) diluted in blocking buffer for 2 hours at room temperature ...
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bioRxiv - Bioengineering 2024Quote: ... Stellate cells were labeled for alpha-smooth muscle actin (α-SMA) (1:100) (Sigma, cat. no. A2547). The primary antibodies were incubated at 4°C overnight ...
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bioRxiv - Cell Biology 2024Quote: ... cells were pulse labeled with CldU for 20 min followed by IdU containing 25 µM hydroxyurea (Sigma) or 25 nM camptothecin (Sigma ...
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bioRxiv - Developmental Biology 2024Quote: ... cells were labeled with 20 mM 5-chloro-2-deoxyuridine (CldU, Sigma-Aldrich by Merck, Darmstadt, Germany) for 20 min ...
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bioRxiv - Microbiology 2020Quote: ... protein complexes were precipitated using Protein A agarose beads (Sigma, St. Louis, MO, USA). The precipitated material was analyzed by western blot ...
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bioRxiv - Animal Behavior and Cognition 2022Quote: The protein content was analysed with the Bicinchonic Acid Protein Assay Kit (Sigma-Aldrich) according to the manufacturer’s protocol with minor adjustments ...
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bioRxiv - Cell Biology 2021Quote: The Protein A slurry was prepared by washing 1.5 g Protein A Sepharose (Sigma) 4 times in 1X Tris Buffered Saline (1X TBS ...
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bioRxiv - Neuroscience 2021Quote: ... Bradford protein assay was used to quantify total protein levels (Bradford, Sigma-Aldrich, USA) and to normalize BDNF data.
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bioRxiv - Physiology 2022Quote: ... Protein carbonylation was assessed with an Oxyblot protein oxidation detection kit (#S7150, Merck Millipore) following the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2024Quote: ... Protein precipitation was carried out using ProteoExtract Protein Precipitation Kit (EMD Millipore, 539180-1KIT) according to the manufacturer’s protocol and submitted for MS analysis to the Taplin Biological Mass Spectrometry Facility.