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Citations for Millipore Sigma :
2751 - 2800 of 10000+ citations for Recombinant Human CD22 protein His tagged APC labeled since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2024Quote: ... or mouse anti-human BCL-XL (EMD Millipore MAB3121) antibodies were added to lysate aliquots and incubated at 4°C overnight ...
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bioRxiv - Immunology 2024Quote: ... human myeloma IgE (0.3 µg/mL, Sigma-Aldrich, #401152) was used for sensitizing the cells in the presence of recombinant human IL-4 (Gibco ...
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bioRxiv - Neuroscience 2024Quote: ... human leukemia inhibitory factor (hLIF, 10 ng/ml, Millipore), SB431542 (2 µM) ...
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bioRxiv - Cancer Biology 2024Quote: ... supplemented with 5% human AB serum (Millipore Sigma, H4522), 2 mM L-glutamine (Gibco ...
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bioRxiv - Molecular Biology 2020Quote: ... protein was isolated from cells using 300μl CytoBuster™ Protein Extraction Reagent (Novagen) per well according to manufacturer’s instructions ...
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bioRxiv - Bioengineering 2022Quote: ... Protein concentrations were determined with the BCA protein assay kit (Novagen, Merck KGaA), and protein homogeneity was analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE ...
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bioRxiv - Plant Biology 2021Quote: ... Protein extracts were performed with the Plant Total Protein Extraction Kit (Sigma, USA). Protein concentrations were measured using the BCA method ...
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bioRxiv - Cancer Biology 2019Quote: ... Lysates were sonicated and protein concentration was determined using BCA protein assay (Sigma). 30 μg of total proteins were loaded on 12% SDS–PAGE gel and separated by electrophoresis ...
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bioRxiv - Evolutionary Biology 2019Quote: ... a marker of protein oxidation (protein carbonyls; OxyBlot; s7150; EMD Millipore, Billerica, MA), and a marker of mitochondrial biogenesis (PGC-1α ...
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bioRxiv - Plant Biology 2020Quote: ... were subjected to protein extraction using Plant Total Protein Extraction Kit (Sigma-Aldrich). The protein concentration was measured using 2-D Quant Kit (GE Healthcare ...
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bioRxiv - Genomics 2019Quote: Duolink PLA kit was used to detect protein-protein interactions (Sigma Aldrich DUO92101). PLA was performed according to manufacturer’s protocol plus additional negative controls ...
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bioRxiv - Genetics 2020Quote: ... Protein concentrations were analyzed with a Bicinchoninic Acid Protein Assay Kit (Sigma-Aldrich), using bovine serum albumin as a standard ...
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bioRxiv - Immunology 2020Quote: Total proteins isolated from BMDCs were lysed in CytoBuster protein extraction reagent (Novagen). Protein quantification was performed using NanoDrop One Spectrophotometer ...
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bioRxiv - Cell Biology 2022Quote: ... the other stained for proteins with SYPRO Red Protein Gel Stain (Sigma Aldrich). Both dyes were used according to the manufacturers’ protocols.
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bioRxiv - Molecular Biology 2024Quote: The protein mixtures were filtered using a low protein binding filter (Millipore Sigma) and purified on a SourceQ15 anion exchange column (Cytiva ...
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bioRxiv - Genomics 2024Quote: ... Protein transfer and total protein content was assessed by Ponceau Staining (Sigma Aldrich). Ponceau Stain was washed out with 1x TBST ...
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bioRxiv - Genetics 2024Quote: ... protein concentration was measured by use of a BCA Protein Assay (SIGMA Aldrich) and samples stored at -80°C.
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bioRxiv - Microbiology 2022Quote: ... total cell protein was measured using the bicinchoninic acid protein assay (Sigma-Aldrich) against bovine serum albumin standards ...
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bioRxiv - Cancer Biology 2023Quote: Oxidized proteins were detected using the OxyBlot Protein Oxidation Detection Kit (Sigma, S7150) and following the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2023Quote: ... The protein concentration was quantified using bicinchoninic acid (BCA) Protein assay (Sigma-Aldrich). Then 60 μg of the total protein of each genotype was separated on 8% SDS-PAGE and transferred to the PVDF membrane (Amersham ...
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bioRxiv - Microbiology 2023Quote: ... 1000 µg of protein extracts were cleared with agarose protein A beads (Millipore) at 4 ℃ for 1.5 h on a rocking platform ...
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bioRxiv - Microbiology 2023Quote: ... or with slurry protein G beads (Sigma-Aldrich Fast Flow Protein G sepharose). For GFP-p65 and GFP-COMMD2 the samples were immunoprecipitated as per manufacturer’s instructions with the elution was recovered in either 5x with Laemmli buffer 74 and boiled at 98°C (dry bath ...
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bioRxiv - Plant Biology 2023Quote: ... the protein extract was incubated with 30 μL Protein A Agarose beads (Millipore), 10 μL anti-SIZ1 antibody with rotation at 4°C for 4 h.
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bioRxiv - Microbiology 2023Quote: ... The protein was purified using a Protein A agarose gravity column (Millipore Sigma) followed by SEC using a S200 column.
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bioRxiv - Biochemistry 2023Quote: ... Protein concentrations were determined with the BCA protein assay kit from Sigma-Aldrich.
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bioRxiv - Biochemistry 2019Quote: 25 µl of 100 µM recombinant purified GST Pat1-NC was bound to 30 µl GST-slurry (Sigma), and one aliquot of GST-beads was included without added Pat1-NC as negative control ...
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bioRxiv - Immunology 2019Quote: ... supplemented with cytokine cocktails aimed at proliferation (50 ng/ml murine recombinant thrombopoietin (TPO, Sigma-Aldrich SRP3236-10UG) and 100ng/ml Stem Cell Factor (SCF) ...
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bioRxiv - Immunology 2019Quote: ... NMRI mice were injected subcutaneously at two different locations with 100µg of the recombinant Thio-EmACT resuspended in 100µl Freund Incomplete adjuvant (Sigma). The double injections were repeated four weeks later to boost the mice anti-EmACT response ...
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bioRxiv - Biochemistry 2022Quote: ... and ∼1 μg recombinant GST or GST-SMN in freshly made 25 mM bis tris propane (Sigma B6755) pH 6.8 buffer with 1 M NaCl and 0.05% NP-40 ...
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bioRxiv - Cancer Biology 2022Quote: ... cells that were cultured in 0.5 % FBS were stimulated with hEGF (recombinant EGF) (Sigma Alrdich, Catalog No: E9644) in concentrations (10 and 30 ng/ml ...
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bioRxiv - Microbiology 2022Quote: ... Assay was conducted according to the manufacturer’s instructions and recombinant L-Lactic Dehydrogenase from porcine heart (Sigma-Aldrich) was used to generate a standard curve ...
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bioRxiv - Cell Biology 2023Quote: ... either coated with BSA or recombinant Laminin332 (BioLamina LN332-0502, diluted in Dulbecco’s Phosphate Buffered Saline, Sigma, D8662) and left to adhere overnight ...
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bioRxiv - Bioengineering 2023Quote: ... mESC maintenance media was supplemented with 1,000 units/mL of Recombinant Mouse Leukemia Inhibitory Factor (Millipore Sigma # ESG1107). Media was changed daily.
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bioRxiv - Biochemistry 2023Quote: His-PP-Pex6 1-204 protein (purified by recombinant expression from plasmid pDC69) was digested with 0.1 or 0.2 μg/μL trypsin protease (Sigma) for 10 minutes at 23 °C in 100 mM Tris pH 8.0 buffer ...
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bioRxiv - Bioengineering 2024Quote: ... Supplemented IVM media consisted of MediCult IVM media (Origio) supplemented with 75 mIU/mL of recombinant FSH (Millipore), 100 mIU/mL recombinant hCG (Sigma) ...
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bioRxiv - Microbiology 2024Quote: ... with 50 μg of either recombinant LMW630 mixed with 200 ng/mouse pertussis toxin (Sigma-Aldrich, MO, USA) for NF10 production or with 50 μg of each of five recombinant LMWs in alum mixed with 200 ng/mouse pertussis toxin (Sigma-Aldrich ...
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bioRxiv - Molecular Biology 2021Quote: Protein-G (Protein G Sepharose® 4 Fast, cat# GE17-0618-01) or Protein-A (ProteinA-Sepharose® 4, cat#P9424 Millipore) beads were washed twice with 1x PBS and twice with IP100 buffer (25 mM Tris-HCl 7.9 ...
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bioRxiv - Neuroscience 2019Quote: ... Membranes were then washed twice 5 min in TBST and incubated with the primary antibody over night at 4°C (human and mouse a-syn: 610786 BD Biosciences; human α-syn: 804-258-1001, Enzo Life Science; beta-actin: A4700, Sigma). After incubation with the first antibody ...
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bioRxiv - Immunology 2022Quote: Concentrations of human serum albumin in human plasma and bronchoalveloar lavage fluid were measured using an ELISA kit from Sigma Aldrich (Cat. #RAB0603) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2022Quote: ... Antibodies for immunostaining included rabbit anti-human/mouse PKD-1 (SAB4502371) and mouse anti-human/mouse α-smooth muscle actin (α-SMA, A2547) (Sigma-Aldrich), mouse anti-human/rat CD44 (5640S ...
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bioRxiv - Developmental Biology 2024Quote: ... anti-Ku80 (CST, #2180, 1:400), anti-human nuclear antigen (Novus NBP2-34342, 1:200) and anti-human-specific Albumin (Sigma, A3293, 1:200). Species-specific secondary antibodies conjugated with Alexa Fluor 555 ...
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bioRxiv - Bioengineering 2020Quote: ... of 0.3 with Edit-R lentiviral mKate2-tagged, constitutively expressed Cas9 nuclease under the hEF1α promoter (Dharmacon, CAS11229) in transduction medium containing 5 μg/mL polybrene (EMD Millipore, TR-1003-G). Complete growth medium was added to the transduced cells at a 3:1 dilution after 5 hours ...
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bioRxiv - Biochemistry 2021Quote: ... mouse APC-conjugated antibody directed against dsRNA J2 (IF 1:200, Scicons, 10010500) mouse anti-3xFlag M2 (WB 1:25000, Sigma, F1804), rabbit anti-Tubulin (WB 1:4000 ...
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bioRxiv - Immunology 2020Quote: Telomere vesicles were extracted from APCs live-labelled with Cy3 TelC telomere probes and the membrane lipid dye PKH67 (Sigma Aldrich). After labelling ...
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bioRxiv - Neuroscience 2023Quote: ... Slides were washed with PBS and incubated overnight with anti-SOX10 (1:200, R&D, AF2864) and anti-APC CC1 (1:200, Millipore, MABC200), followed by labeling with Alexa Fluor-conjugated secondary antibodies (2 μg/mL ...
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bioRxiv - Cell Biology 2019Quote: ... the membranes were incubated overnight at 4°C with the adequate primary antibodies in TBST buffer: mouse anti-His-tag (1:2000, Millipore, 05-949), rabbit anti-CAP-D2 serum (1:1000 ...
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bioRxiv - Cell Biology 2021Quote: ... ICR1-His6 and ICR2-His6 were stained with an anti-His antibody (1:5,000) and secondary antibody conjugated with fluorescein (Sigma; F0257; 1:5,000). The solution was then centrifuged at 12,000 g for 5 min ...
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bioRxiv - Microbiology 2020Quote: ... Cells were routinely passaged in Dulbecco’s modifies Eagle medium (DMEM+) culture media with serum (DMEM, low glucose, from Sigma-Aldrich; 10% HI-FBS; 1% penicillin-streptomycin solution from Sigma-Aldrich; 1% 200mM L-glutamine from Sigma-Aldrich) ...
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bioRxiv - Plant Biology 2021Quote: ... were inserted between the NdeI and XhoI sites of the bacterial expression vector pET-24a containing a HIS-tag (Novagen, Madison, USA). The ligated products were transformed into TOP10 E ...
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bioRxiv - Microbiology 2021Quote: ... from ffmA was PCR amplified and cloned in frame as a NdeI/HindIII fragment upstream of the C-terminal 6x-His tag in pET29a+ (EMD Millipore, Inc.) to form plasmid pSP113 ...