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Citations for Millipore Sigma :
2801 - 2850 of 3691 citations for CD40 Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2021Quote: ... shRNAs stably integrated in mouse and human cells were selected with 10 and 2 μg/ml puromycin (Sigma), respectively ...
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bioRxiv - Cancer Biology 2020Quote: ... Human colon cancer cell lines HCT116 TP53+/+ and HCT116 TP53-/- were grown in McCoy’s 5A Medium (Sigma, M9309) supplemented with 10% FBS and Pen/Strep ...
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bioRxiv - Microbiology 2020Quote: ... Bound antibody was detected with an anti-human antibody conjugated to horseradish peroxidase (HRP; EMD Millipore, Burlington, MA) and Ultra-TMB substrate (ThermoFisher) ...
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bioRxiv - Cell Biology 2021Quote: ... α-taxilin (NM_175852.4) and its truncated segments were amplified from human cDNA and cloned into pcDNA3.1(+) (Sigma-Aldrich). Full-length α-taxilin and γ-taxilin were also cloned into p3×FLAG-CMV-14 (Sigma-Aldrich) ...
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bioRxiv - Cell Biology 2021Quote: ... HeLa D2 parental control cells were transfected with 300 ng/ml human VRK1 MISSION® esiRNA (EHU119541, Sigma) using HiPerFect Transfection Reagent (301707 ...
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bioRxiv - Microbiology 2021Quote: ... HMEC-1 cells (human microvascular endothelial cells RRID:CVCL_0307) were grown in HMEC media (MCDB 131 media (Sigma, M8537) supplemented with 10% FBS (HyClone ...
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bioRxiv - Microbiology 2021Quote: Calu-3 (human epithelial lung adenocarcinoma) cells were cultured in Minimum Essential Medium Eagle (MEM, Sigma, Cat#M4655) supplemented with 10% (upon and after viral infection ...
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bioRxiv - Microbiology 2021Quote: ... Then the inoculum was aspirated and DMEM supplemented with 5% NBCS and 7.5 µg/mL human immunoglobulin (Sigma) was added ...
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bioRxiv - Immunology 2021Quote: ... Serum IgG subclasses were tested with isotype-specific murine anti-human IgG subclasses (Sigma-Aldrich, S:t Louis, MA).
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bioRxiv - Microbiology 2021Quote: Calu-3 (human epithelial lung adenocarcinoma) cells were cultured in Minimum Essential Medium Eagle (MEM, Sigma, Cat#M4655) supplemented with 10% (upon and after viral infection ...
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bioRxiv - Microbiology 2021Quote: ... and differentiating cells for at least 7 days in RPMI supplemented with 10% Human AB Serum (Millipore Sigma), 100U/mL P/S ...
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bioRxiv - Microbiology 2021Quote: ... resolved by SDS–PAGE and analysed by immunoblotting using primary antibodies specific for human DDX42 (HPA023571, Sigma-Aldrich), Flag (M2 ...
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bioRxiv - Microbiology 2021Quote: ... Human HeLa cells (ATCC CCL-2) were maintained in Minimum Essential Medium Eagle (MEM) (Sigma-Aldrich, Taufkirchen, Germany), 7% FBS and 1% MEM non-essential amino acid solution ...
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bioRxiv - Immunology 2022Quote: Human THP-1 monocytes were differentiated into macrophages by 24 hr incubation with 100 nM PMA (Sigma-Aldrich) in complete RPMI medium at 1.6 × 105 cells/well in 12-well plates ...
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bioRxiv - Bioengineering 2022Quote: ... suspended in a solution prepared in the following order: 30 μL of human male AB serum (Sigma-Aldrich), 60 μL human plasma fibronectin (EMD Millipore) ...
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bioRxiv - Bioengineering 2022Quote: ... KCs were cultured and serially passaged into 7 μg/cm2 human collagen IV-coated plates (#C7521; Sigma-Aldrich). All cell sources were obtained at Yale-New Haven Hospital under protocols approved by the Yale University Institutional Review Board including ...
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bioRxiv - Bioengineering 2022Quote: ... Epidermal growth factor (EGF) and Recombinant Human Fibroblast Growth Factor Basic (FGF2) Progesterone were purchased from Sigma-Aldrich. OPSS-PEG-SVA (3400 Da) ...
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bioRxiv - Biophysics 2022Quote: ... for mouse ILC2s and human peripheral ILC2s was supplemented with MEM nonessential amino acid solution (M7145, Sigma-Aldrich), 10 mM HEPES buffer (H3537 ...
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bioRxiv - Immunology 2022Quote: ... Human Umbilical Vein Endothelial Cells (HUVEC) were grown in 1% gelatin coated wells in M199 medium (Sigma-Aldrich) containing 20% fetal bovine serum (FBS) ...
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bioRxiv - Biochemistry 2020Quote: ... Human Rab8a 6-176aa (referred to as Rab8a)-encoding DNA was cloned into a pet51b(+) vector (Merck Millipore), resulting in a construct with a N-terminal Strep® II tag and enterokinase cleavage site and a C-terminal 10xHis tag ...
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bioRxiv - Developmental Biology 2021Quote: Human iPSCs were transduced in mTESR in the absence of antibiotics and with 6 μg/mL polybrene (Millipore). The lentiviruses containing the GOIs were transduced using 10 MOI in the presence of the reverse tetracycline transactivator M2rtTA (MOI 10) ...
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bioRxiv - Genomics 2019Quote: ... was cultured in Roswell Park Memorial Institute (RPMI) 1640 medium supplemented with 10% AB human serum (Sigma Aldrich), 100 U/mL recombinant human IL-2 (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2021Quote: Cell lines and isolated primary human immune cells were treated with etoposide (ETP, Sigma-Aldrich, cat. number E1383) at the indicated concentrations from a 50 mM stock that was prepared from a lyophilized powder reconstituted in DMSO (Sigma-Aldrich ...
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bioRxiv - Microbiology 2020Quote: ... We detected bound antibody with horseradish peroxidase (HRP)-coupled anti-human (Fc) antibody (Sigma Aldrich catalog number A0170).
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bioRxiv - Cell Biology 2020Quote: Human monocyte-derived macrophages were separated from healthy donor buffy coats by centrifugation through a Histopaque 1077 (Sigma) gradient and adhesion purification ...
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bioRxiv - Immunology 2020Quote: ... Cell enrichment buffer consisted of 0.22 μm filtered PBS supplemented with 0.5% heat inactivated human AB serum (Sigma).
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bioRxiv - Biophysics 2021Quote: ... Samples were stained using a 1:100 dilution of the primary antibodies (rabbit polyclonal anti-human IRSp53, Sigma and rabbit polyclonal anti-human IRTKS antibody ...
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bioRxiv - Immunology 2021Quote: ... Cells were cultured in Human Plasma-like Media (HPLM) (8) + 1% pen/strep + 5% dialyzed serum (Sigma Aldrich). For SARS-CoV-2 peptide stimulation ...
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bioRxiv - Microbiology 2022Quote: ... The Madin Darby canine kidney (MDCK) and human embryonic kidney (HEK) 293T cells (ATCC) were maintained with Dulbecco’s Modified Eagle’s medium (DMEM) (Sigma), supplemented with 10% fetal calf sera (FCS ...
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bioRxiv - Immunology 2019Quote: ... Total antibody concentration between determinants was controlled by addition of human IgG2/kappa where needed (Sigma Aldrich; AG504). Cells were washed with ice-cold PBS and added to dengue immune complexes (MOI 5) ...
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bioRxiv - Cancer Biology 2020Quote: ... 10 mmol/L HEPES (PAA Laboratory) and 1 ng/mL human basic fibroblast growth factor (bFGF) (Sigma-Aldrich) (Lonza ...
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bioRxiv - Genomics 2020Quote: Human induced pluripotent stem cells (hiPSCs) were thawed with mTeSR1 (STEMCELL, #85850) and 1 μM H1152 (Millipore, #555550), seeded on Matrigel (Corning ...
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bioRxiv - Physiology 2021Quote: ... C2C12 myotubes were cultured in the differentiation medium containing 2 or 200 nM human insulin (Cat.# I9278, Sigma) for 16 hours prior to reaching day 10 (Fig.1A) ...
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bioRxiv - Immunology 2020Quote: ... For IgM antibody detection biotinylated anti-human IgM (affinity purified, μ-chain specific, Sigma-Aldrich cat. Nr. B1140) was used as secondary antibody.
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bioRxiv - Immunology 2020Quote: ... For IgM antibody detection biotinylated anti-human IgM (affinity purified, μ-chain specific, Sigma-Aldrich cat. Nr. B1140) was used as a secondary antibody ...
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bioRxiv - Immunology 2020Quote: ... Transduced CD8+ T cells were expanded in the presence of 10 U/ml recombinant human IL-2 (Sigma) Human CD8+ T cells were purified from donor PBMCs (Cambridge Bioscience or NHSBT ...
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bioRxiv - Cancer Biology 2019Quote: ... ECM proteins (collagen I and natural mouse laminin were purchased from Life Technologies, human plasma fibronectin from Millipore, human collagen IV from Sigma-Aldrich ...
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bioRxiv - Microbiology 2021Quote: Overnight cultures were standardised to an OD600nm of 0.1 and incubated in 10% pooled human serum (Sigma Aldrich) diluted in PBS for 90 min at 37°C with shaking ...
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bioRxiv - Cell Biology 2021Quote: The digoxigenin-labeled antisense probe targeting bases 32-500 of human GNRH1 mRNA (NM_001083111.2) was transcribed in the presence of digoxigenin-11-UTP (Merck Millipore) in a reaction mixture containing linearized cDNA template (1 µg) ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... The antigen-antibody complexes were detected using peroxidase-conjugated goat anti-human or goat anti-mouse IgG (Sigma) and revealed using the enhanced chemiluminescence (ECL ...
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bioRxiv - Cell Biology 2021Quote: Human muscles were dissociated and digested with pronase enzyme (1.5mg/mL; protease from streptomyces griseus P5147-5G Sigma) in DMEM at 37°C for 20 min and passed through a 100 mm cell strainer (repeated 4 times) ...
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bioRxiv - Genomics 2021Quote: ... and VEGF) was measured in plasma using a non-human primate multiplexing bead immunoassay (Millipore-Sigma, Burlington, MA) according to the manufacturer’s protocol ...
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bioRxiv - Genomics 2021Quote: ... and VEGF) was measured in plasma using a non-human primate multiplexing bead immunoassay (Millipore-Sigma, Burlington, MA) according to the manufacturer’s protocol ...
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bioRxiv - Genomics 2021Quote: Human osteosarcoma U2OS cells (ATCC® HTB-96™) were cultivated in Dulbecco’s Modified Eagle’s Medium (DMEM) (Sigma) with 10% fetal bovine serum (Lonza) ...
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bioRxiv - Developmental Biology 2021Quote: ... Human NTOs were permeabilized and blocked using .3% Triton X (PanREAC AppliChem) and 0.5% BSA solution (Sigma-Aldrich) for 30 m ...
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bioRxiv - Cell Biology 2020Quote: ... 25×106 cells were plated on 6-well culture dishes coated with human fibronectin (Sigma, St Louis, MO) and maintained in endothelial cell medium-2 (EGM-2 -Clonetics ...
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bioRxiv - Cell Biology 2020Quote: ... the cell medium was replaced with DMEM containing 0.2% FBS and 300ng/mL IGF-1 (human recombinant, Sigma) for 3 hours ...
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bioRxiv - Microbiology 2020Quote: ... Serum susceptibility was determined by incubation of late log-phase cells with 66 % normal human serum (Sigma-Aldrich) as described (Short et al ...
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bioRxiv - Cell Biology 2020Quote: ... rat anti-human RIPK3 (clone 1H2; produced in-house5 and available as Millipore MABC1640; 1:2000/1:400), rabbit anti-phospho-S227 human RIPK3 (Cell Signaling Technology ...
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bioRxiv - Molecular Biology 2022Quote: Human embryonic kidney (HEK) 293T cells (ATCC, CRL-3216) were cultured in DMEM (high glucose, Sigma-Aldrich, SH30022.01) with 10% FCS (Hyclone ...