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Citations for Millipore Sigma :
2701 - 2750 of 3691 citations for CD40 Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2019Quote: Hormone secretion was quantified by Luminex assay (Human Metabolic Hormone Magnetic Bead Panel, HMHEMAG-34K, EMD Millipore) according to manufacturer’s standard protocol ...
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bioRxiv - Cell Biology 2020Quote: PC-9 (human lung cancer) cells were cultured in RPMI-1640 medium (Sigma-Aldrich, R8758-500 mL) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Neuroscience 2020Quote: ... remaining archived samples were processed using a non-human primate multiplexing bead immunoassay (Milipore-Sigma, Burlington, MA). The kit was run according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: Lentivirus based knockdown of human ATG16L1 (5’-ACTGTAGCTTTGCCGTGAATG-3’) was performed using MISSION shRNA constructs (Sigma-Aldrich) and psPAX2 packaging system ...
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bioRxiv - Cell Biology 2020Quote: ... We found that an additional surface treatment of 4 µg/cm2 human plasma Fibronectin (Millipore, Billerica MA) in DMEM applied overnight at 37°C with mild orbital plate agitation was necessary to create a consistent surface coating for cell adhesion ...
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bioRxiv - Microbiology 2019Quote: Human embryonic kidney HEK-293T were maintained in DMEM supplemented with 10% fetal bovine serum (Sigma F8067) and gentamycin (Gibco) ...
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bioRxiv - Immunology 2021Quote: ... human metastatic melanoma cell line A2058 (ECACC Cat# 91100402, RRID:CVCL_1059, human colon carcinoma cell line MDST8 (ECACC Cat# 99011801, RRID:CVCL_2588) purchased from Sigma-Aldrich in 2018 ...
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bioRxiv - Immunology 2021Quote: ... Plates were washed and bound human ACE2 was detected using HRP-conjugated anti-Flag antibody (Sigma-Aldrich, cat ...
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bioRxiv - Immunology 2020Quote: ... A 6X 2-fold serial dilution (8000-250 μg/mL) of human hemoglobin (Sigma, St. Louis, MO) was prepared ...
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bioRxiv - Microbiology 2021Quote: ... of human DDX21 isoform 1 (NM_004728.4) and isoform 2 (NM_001256910.1) into plasmid p3XFLAG-CMV-14 (Sigma-Aldrich), respectively ...
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bioRxiv - Molecular Biology 2021Quote: ... The human keratinocyte cell line HaCaT was cultured in Dulbecco’s modified Eagle’s medium (DMEM, Sigma-Aldrich, D6419) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Microbiology 2021Quote: ... Human monocyte cell line U937 cells were maintained in Roswell Park Memorial Institute 1640 media (Sigma-Aldrich) supplemented with 10% FCS (Fetal calf serum ...
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bioRxiv - Immunology 2020Quote: ... MISSION shRNA Lentiviral Transduction Particles against human CLPP (TRCN0000291174) or eGFP (RHS4459) were purchased from Sigma-Aldrich and Horizon Discovery respectively ...
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bioRxiv - Immunology 2020Quote: ... Human monocytic cell line (THP-1) cells were differentiated with 100 nM phorbol myristate acetate (Sigma-Aldrich) for 3 days and then the same polarization protocol was performed ...
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bioRxiv - Immunology 2021Quote: ... conv CD4+ cells were activated with 1μg/ml of soluble anti-human CD3 /OKT3 mAb (Sigma, USA) in the presence of irradiated allogenic PBMC (∼50,000 ...
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bioRxiv - Genetics 2019Quote: Small-interfering RNAs (siRNA) targeting human C/EBPβ were purchased from Sigma Aldrich (St Louis, MO, USA). siRNA ID ...
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bioRxiv - Cancer Biology 2021Quote: Immortalized Human Hepatocytes (IHH) were cultured under standard conditions by using control media DMEM/F-12 (Sigma) without phenol red ...
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bioRxiv - Genomics 2020Quote: ... the cells were spun down and moved to “Phase 2” IMDM media containing 5% human serum (Sigma), 330µg/mL transferrin ...
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bioRxiv - Cancer Biology 2022Quote: ... Detection of proteins was performed by using primary rabbit anti-human hepsin antibody (Sigma Aldrich, Steinheim, Germany), pAKT (Invitrogen ...
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bioRxiv - Cancer Biology 2022Quote: MG63 and U2OS human OS cell lines (#86051601-1VL and #92022711-1VL, Sigma, St. Louis, MO, USA), and a patient-derived xenograft (PDX ...
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bioRxiv - Cell Biology 2022Quote: ... falciparum 3D7 wild type cell line and the 3D7_SLI_slp-gfp_glmS (Slp) strain were cultured in 0+ human red blood cells using RPMI 1640 medium (Sigma) with added 0.2 mM hypoxanthine ...
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bioRxiv - Neuroscience 2022Quote: ... C3 and C1 were analyzed with HCMP2MAG-19K-02 Human Complement Magnetic Bead Panel 2 (Merck Millipore). Supernatants from non-stimulated CTL and L2-PD astrocytes cultured for 14 days were collected and stored at −80°C for long storage ...
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bioRxiv - Microbiology 2022Quote: ... inserts were coated with an extracellular matrix component mixture comprised of 10 μg/mL human fibronectin (Sigma), 100 μg/mL bovine serum albumin (Fisher) ...
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bioRxiv - Systems Biology 2023Quote: ... CD8+ T-cell cultures were supplemented with 120 IU/ml human recombinant IL-2 (Sigma Aldrich, USA). T-cells were aliquoted into five samples with 1.5 x 106 cells in each condition to obtain cells at five different time points (6 hours ...
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bioRxiv - Molecular Biology 2022Quote: Mouse RAW macrophages or human THP1 monocytes (with 50ng/mL phorbol 12-myristate 13-acetate, PMA, Sigma) were plated in 12-well plates (5×105 cells/mL ...
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bioRxiv - Microbiology 2024Quote: ... and 12.5 μl of the neat eluate or negative control commercially available purified human IgG (Sigma, I2511) in PBS at 200 μg/ml was used to resuspend the cells ...
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bioRxiv - Microbiology 2024Quote: ... or a non-target negative control gRNA (Human Sanger Arrayed Whole Genome Lentiviral CRISPR library, Sigma-Aldrich) using polyethylenimine (PEI ...
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bioRxiv - Neuroscience 2024Quote: Total RNA was isolated from human and animal PFC samples using TRIzol Reagent (Sigma-Aldrich, Taufkirchen, Germany). To ensure proper handling of the RNA ...
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bioRxiv - Neuroscience 2024Quote: The inhibitory activities of 5–7 and all the reference compounds were determined using modified Ellman’s method [25,42,43] against human recombinant AChE (hAChE, E.C. 3.1.1.7, purchased from Sigma-Aldrich, Prague, Czech Republic) and human plasmatic BChE (hBChE ...
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bioRxiv - Microbiology 2024Quote: ... Binding was detected by incubating the plates sequentially with HRP-linked rabbit anti-human IgG (A8792, Sigma). After washing ...
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bioRxiv - Microbiology 2024Quote: ... and approximately 105 CFU mixed with human serum from male AB plasma (Sigma-Aldrich, product number H4522) in a total volume of 200 µl ...
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bioRxiv - Cell Biology 2023Quote: ... then incubated with Protein A Agarose/Salmon Sperm DNA and 5 μg human IgG (Sigma, Cat# I4506) or PPAR-γ Antibody (E-8 ...
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bioRxiv - Cancer Biology 2023Quote: ... Albumin was measured in the cell culture medium by Human ALB/Serum albumin ELISA Kit (Sigma-Aldrich) and in the cell lysate by qPCR ...
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bioRxiv - Physiology 2023Quote: ... Immortalized human LX-2 cells (MilliporeSigma, Burlington, VT, USA) were cultured in phenol-free DMEM (Sigma-Aldrich) containing 2% FBS (Gibco ...
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bioRxiv - Bioengineering 2023Quote: ... Membranes were blocked with 5% milk powder and incubated with rabbit anti-human Sox2 (Sigma-Aldrich, US), rabbit anti-human Oct4 (Millipore ...
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bioRxiv - Cancer Biology 2023Quote: ... the sections were incubated with rabbit anti-human antibody directed against CaMKK2 (HPA017389, Sigma, St. Louis, MO) at 1:500 ...
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bioRxiv - Neuroscience 2023Quote: Whole cell lysates from murine and human hippocampus were generated by adding RIPA lysis buffer (Sigma-Aldrich) supplemented with protease inhibitors (Protease Inhibitor Cocktail ...
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bioRxiv - Bioengineering 2023Quote: ... Human M0 macrophages were differentiated from THP1 monocytes by incubating cells with 100 ng/mL PMA (Sigma) for 24 hrs ...
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bioRxiv - Pathology 2023Quote: ... or 2 mg of human IgG isotype (n = 4) (I4506-100MG, Millipore-Sigma, Saint Louis, MO,USA) were injected subconjunctivally in the upper eyelid of eyes immediately after the post-alkali exposure irrigation ...
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bioRxiv - Pathology 2023Quote: ... or 2 mg of human IgG isotype (n = 4) (I4506-100MG, Millipore-Sigma, Saint Louis, MO,USA) were injected subconjunctivally in the upper eyelid of eyes immediately after the post-alkali exposure irrigation ...
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bioRxiv - Cancer Biology 2022Quote: PSCs were treated in Matrigel in 5%FBS DMEM with 20 ng/mL human TGFβ1 (T7039; Sigma), 300 nM Neratinib (S2150 ...
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bioRxiv - Cancer Biology 2022Quote: PSCs were treated in Matrigel in 5%FBS DMEM with 20 ng/mL human TGFβ1 (T7039; Sigma) for 24 h ...
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bioRxiv - Molecular Biology 2023Quote: ... A lentivirus plasmid vector containing an shRNA insert not targeting human and mouse genes (SHC002, Sigma-Aldrich) was used as a non-target control (shCTL).
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bioRxiv - Molecular Biology 2023Quote: ... EpiLC transition was induced by plating 1×103 naive mESCs on a human plasma fibronectin (Millipore, FC010) coated plate in N2B27 medium supplemented with 20 ng/mL activin A (SCI) ...
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bioRxiv - Neuroscience 2023Quote: ... Thorough washing was followed by blocking for 1 hour in 1% human serum albumin (HSA; Sigma-Aldrich) and 0.03-0.1% Triton X-100 dissolved in TBS ...
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bioRxiv - Developmental Biology 2023Quote: ... 400 µl of solution of recombinant TnC (Supplementary Materials and Methods) or purified human TnC (Merck Millipore) (15 µg/ml in 0.01% PBS-Tween ...
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bioRxiv - Genomics 2023Quote: ... Thawed PBMCs were quenched by adding 12 mL 37°C pre-warmed human serum (Sigma Aldrich, # H4522) and were centrifuged at 500 x g for 5 min at room temperature ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... The human M2R and M5R were stimulated with Acetylcholine (ACh; Sigma-Aldrich, A6625, dissolved in measuring buffer).
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bioRxiv - Bioengineering 2023Quote: ... 5×104 human MDMs or murine (NSG) BMDMs were co-cultured with 5×104 PKH26-labeled (Sigma) tumor cells in the presence of the different recombinant SiRPα decoys at 10ug/ml in serum-free medium in 96-well ultra-low adherent plates (Corning ...
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bioRxiv - Developmental Biology 2023Quote: ... Cells were sub-cultured on days 4 and 8 of differentiation onto human fibronectin (Merck Millipore, #FC010) coated plates at a ratio of 1:4 ...