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Citations for Millipore Sigma :
2751 - 2800 of 10000+ citations for Recombinant Human PPAR gamma Protein His tag since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2022Quote: ... Total protein concentration was determined using the Bicinchoninic Acid Protein Assay Kit (Sigma-Aldrich Cat# BCA1) as per manufacturer’s protocol ...
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bioRxiv - Cell Biology 2021Quote: ... Protein-protein interactions were detected using the Detection Kit Red (DUO92008) according to the manufacturer’s (Sigma) instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... and 100 µg protein was incubated with 20 µl packed protein G-sepharose beads (Sigma P3296) at 4°C for 1 hr for preclearing ...
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bioRxiv - Cell Biology 2021Quote: ... Protein concentration of the collagen was verified by both BCA Protein assay (Sigma-Aldrich, cat: BCA1) and drying via vacuum centrifugation.
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bioRxiv - Cell Biology 2022Quote: ... and the protein concentration of the supernatant assessed by Pierce 660nm protein assay reagent (Sigma, 22660). All samples were diluted to a final 1X Laemmli sample buffer (40% glycerol ...
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bioRxiv - Plant Biology 2022Quote: ... Protein concentration was determined in the supernatants using Bradford protein assay method (Bradford reagent, Sigma–Aldrich), a standard curve was performed using BSA (bovin serum albumine ...
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bioRxiv - Biophysics 2023Quote: ... The fusion protein was treated with SUMO protease (Sigma-Aldrich SAE0067, 5 U/ mg target protein) to liberate 3CLpro with authentic N- and C-termini (Ser1 and Gln306 ...
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bioRxiv - Microbiology 2024Quote: ... The protein pellet was washed in protein wash buffer (0.3 M guanidine hydrochloride (Sigma Aldrich, USA) in 95% ethanol ...
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bioRxiv - Physiology 2023Quote: ... The total protein content in samples was obtained using a BCA protein assay kit (Sigma-Aldrich) with the SpectraMax Paradigm microplate reader (Molecular Devices ...
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bioRxiv - Biochemistry 2022Quote: ... AviTagged trimeric S and RBD proteins were biotinylated using the Enzymatic Protein Biotinylation Kit (Sigma-Aldrich).
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bioRxiv - Cell Biology 2022Quote: ... 9 mg of protein were mixed with 20 µL of S-protein Agarose beads (#69704, Novagen), that had been previously washed in extraction buffer with 1% (w/v ...
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bioRxiv - Neuroscience 2024Quote: ... After determining the protein concentration through a Bradford protein assay using Bradford reagent (Sigma, cat# B6916), each sample was diluted with an appropriate volume of RIPA buffer mixed with 1x Halt protease & phosphatase inhibitor cocktail to achieve a final protein amount of 20 µg protein ...
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bioRxiv - Microbiology 2024Quote: ... The FLAG-tagged bait protein and any interacting proteins were purified using anti-FLAG resin (Sigma). Briefly ...
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bioRxiv - Molecular Biology 2024Quote: ... The protein complexes were immunoprecipitated by incubation with 20 µL prewashed Protein A agarose beads (Millipore) for 90 min at 4°C ...
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bioRxiv - Biophysics 2024Quote: ... Total of loading protein ware visualized by staining of protein-blotted membrane with ponceau solution (Sigma). Membranes were probed with anti-PPARγ antibody (CST ...
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bioRxiv - Biochemistry 2021Quote: ... His-tagged CLIP-170 fragments and mutants were expressed in BL21 (DE3) and purified by the standard His-tagged purification protocol from Novagen (69670-5, Sigma-Aldrich) with the following modifications ...
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bioRxiv - Genomics 2022Quote: ... or T-175 flasks (Hi-C, CUT&RUN) at a density of 1 × 105 cells/mL and treated with 25 nM PMA (Sigma-Aldrich, cat # P1585-1MG). After 24h ...
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bioRxiv - Biochemistry 2023Quote: Thermal stability of BSA and HSA variants (His-tagged form) and of commercial wild type HSA and BSA (Sigma-Aldrich A1653 and A7638, respectively) was measured by nanoscale differential scanning fluorimetry (nanoDSF) ...
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bioRxiv - Plant Biology 2024Quote: ... One gel was stained with coomassie brilliant blue to reveal MBP and MBP-BIM1 and the other was transferred to a PVDF membrane perform western blot analysis using primary antibody anti-HIS made in mouse (SIGMA, H1029 dilution 1:3000) and the secondary antibody anti mouse IgG used for the CoIPs.
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bioRxiv - Cancer Biology 2021Quote: ... human epidermal growth factor (EGF, 20 ng/ml; Sigma-Aldrich, E9644), human fibroblast growth factor (FGF ...
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bioRxiv - Cancer Biology 2021Quote: ... Human interleukin-2 (IL-2) (H7041) was purchased from Sigma Aldrich. Ficoll-PaqueTM PLUS (17-1440-02 ...
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bioRxiv - Cell Biology 2020Quote: ... 10 ug/ml of Ephrin-A5/Fc (chimera human, Sigma, E0628) and 10 ug/ml of Fc are mixed with 2.5 ug/ ml of Anti-human IgG (Fc specific ...
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bioRxiv - Cell Biology 2020Quote: ... rabbit anti-human MLKL (Sigma-Aldrich M6697; 1:1000/1:200), rabbit anti-RIPK3 (ProSci #2283 ...
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bioRxiv - Immunology 2021Quote: ... with 0.05% human serum albumin (HSA, Sigma-Aldrich, St. Louis, MO) added to the isolation buffer ...
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bioRxiv - Microbiology 2021Quote: ... Primary antibody was diluted in 1% human AB serum (Sigma-Aldrich) in PBS at the concentrations in Table 2 and incubated overnight ...
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bioRxiv - Developmental Biology 2021Quote: ... Forty-five µl of human tubal fluid medium (HTF; Millipore Sigma) containing 4 mg/ml BSA (HTF-BSA ...
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bioRxiv - Developmental Biology 2022Quote: ... and 48 hours later 5 IU human chorionic gonadotropin (hCG, Sigma). Then females were mated with male Hbxip+/- mice overnight ...
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bioRxiv - Genomics 2020Quote: Genomic DNA was isolated from human dermal fibroblasts (106-05A,Sigma) transfected with Dharmacon siRNA cocktails as follows ...
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bioRxiv - Immunology 2021Quote: ... mouse anti-human/mouse SMA-Cy3 (1:200, #C6198, Sigma Aldrich), rat anti-mouse CD35 (1:100 ...
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bioRxiv - Neuroscience 2020Quote: ... 25μg/ml human insulin (Sigma-Aldrich, MO, US; Cat. No. #I9278) and 100 nM RA (Sigma-Aldrich ...
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bioRxiv - Genomics 2020Quote: ... The Milliplex Map Human TH17 Magnetic Bead Panel (Millipore, Burlington, MA) was used to measure IL-33 levels in thawed supernatants at the University of Chicago Immunology Core facility using standard protocols ...
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bioRxiv - Cell Biology 2020Quote: ... full length CRMP5 (DPYSL5, amplified from human brain cDNA library, Novagen), Ephrin receptor A1 cytoplasmic tail (aa 568-976 ...
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bioRxiv - Evolutionary Biology 2021Quote: ... immediately transferred in 250 μl human tubular fluid medium (Millipore, Billerica), punctured with a needle and placed at 37°C ...
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bioRxiv - Cancer Biology 2021Quote: ... and 200 μg/mL human placenta collagen type IV (Sigma-Aldrich) and 100μg/mL Matrigel (Corning ...
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bioRxiv - Immunology 2021Quote: ... or 1:5000 HRP-conjugated anti-human IgM antibody (Sigma #AP114P) was added to each well ...
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bioRxiv - Immunology 2021Quote: ... Anti-Human IgG −Peroxidase antibody (Fab specific) produced in goat (Sigma) diluted 1:45000 in sample buffer was then added and samples were incubated for 1 h at 37°C ...
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bioRxiv - Neuroscience 2020Quote: ... Primary antibodies against mouse or human laminin (1:300 rabbit, Sigma), mouse collagen IV (EMD Millipore) ...
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bioRxiv - Systems Biology 2020Quote: ... containing 1% non-autologous human plasma (Sigma-Aldrich, St Louis, USA), 2 mM L-glutamine (Lonza) ...
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bioRxiv - Bioengineering 2020Quote: ... coated with 50 μg/mL collagen from human placenta (Sigma Aldrich) at ~10,000 cells/cm2 ...
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bioRxiv - Bioengineering 2021Quote: ... 10 ng/mL luteinizing hormone from human pituitary (LH, Sigma, L6420), 1 μM metribolone (Toronto Research Chemicals ...
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bioRxiv - Biochemistry 2020Quote: Alpha-1-acid glycoprotein (AGP) purified from human serum (Sigma #G9885) and A/Philippines/2/1982 (Phil82 ...
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bioRxiv - Biochemistry 2020Quote: PDP-23 was mixed with human pooled male serum (Sigma-Aldrich) to a final concentration of 50 µg/mL and incubated at 37 °C ...
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bioRxiv - Cell Biology 2021Quote: In human HEK CLASH beads conjugated with anti-Flag M2 (Sigma), in FLASH Protein A agarose conjugated with IgG and anti-FBL AB were used ...
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bioRxiv - Microbiology 2020Quote: ... human serum and endothelial growth supplement were from Sigma (Taufkirchen, Germany).
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bioRxiv - Microbiology 2021Quote: ... Cells were then stained with anti-human-IgG-FITC (Sigma, F9512) or anti-mouse-IgG-FITC (Sigma ...
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bioRxiv - Cancer Biology 2021Quote: ... and 200 μg/mL human placenta collagen type IV (Sigma-Aldrich) and 30μg/mL human plasma fibronectin (Corning ...
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bioRxiv - Microbiology 2020Quote: ... Results were normalized to ng actin using Human DNA standard (Sigma). Primers used for quantification of actin were ...
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bioRxiv - Microbiology 2020Quote: ... Human mAbs were concentrated using Amicon Ultra-4 5 kDa (Millipore). Antibody concentration was determined by absorbance measurement at 280 nm using a Nanodrop and purity was determined using SDS-PAGE ...
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bioRxiv - Cell Biology 2020Quote: Human gastric tissues were fixed in 4% paraformaldehyde (PFA – Sigma-Aldrich) for 2 hours and embedded in paraffin wax ...
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bioRxiv - Cancer Biology 2021Quote: ... and anti-human CD28 antibody (5μg/ml) (Millipore #MABF408, Burlingtone, MA) simultaneously for 2 hours in 5% CO2 and air at 37°C ...