Labshake search
Citations for Millipore Sigma :
2651 - 2700 of 10000+ citations for Recombinant Human PPAR gamma Protein His tag since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Bioengineering 2024Quote: ... AC16 human cardiomyocyte cell line was purchased from Sigma Aldrich and cultured according to supplier specifications ...
-
bioRxiv - Cancer Biology 2024Quote: ... except with additional 5 μg/ml human insulin (Sigma-Aldrich) and 1 mM sodium pyruvate (Sigma-Aldrich) ...
-
bioRxiv - Cancer Biology 2024Quote: ... The shRNAs targeting human MARCHF8 were purchased from Sigma-Aldrich. The sgRNAs targeting the mouse Marchf8 were designed by the web-based software ChopChop 113 ...
-
bioRxiv - Cancer Biology 2024Quote: ... anti-Human mitochondria (mouse, Merck Millipore, 1:100, cat#MAB1273), anti-Ki67 (mouse ...
-
bioRxiv - Developmental Biology 2024Quote: ... Ovulation was induced in females using human chorionic gonadotropin (Sigma) according to (Sive ...
-
bioRxiv - Molecular Biology 2020Quote: ... Expression of AsCas12a nuclease in the stable HEK 293 T-REx cell line was induced by 100 ng/mL tetracycline for 24 h and assayed by Western immunoblotting using FLAG-tag specific antibodies (Sigma-Aldrich, F1804).
-
bioRxiv - Immunology 2022Quote: ... with addition of Phos-tag Acrylamide (Wako Chemicals, AAL-107) to a final concentration of 30uM and manganese chloride(II) (Sigma-Aldrich, #63535) to 60uM ...
-
bioRxiv - Microbiology 2021Quote: ... The following antibodies were used: DYKDDDDK Tag Antibody (D6W5B rabbit mAb #14793; Cell Signaling Technology; it binds to the same epitope as Sigma’s Anti-FLAG M2 Antibody ...
-
bioRxiv - Biochemistry 2021Quote: ... Carboxyl-terminal histidine or N-terminal glutathione-S-transferase fusion tags were added using the respective plasmids pET21b (Novagen, Madison, Wisconsin, USA) and pGEX-4T3 (Amersham Biosciences ...
-
bioRxiv - Cell Biology 2021Quote: ... and Spo74-mScarlet were generated at their endogenous genome locus by transformation with PCR tagging cassettes of indicated C-terminal tags using the PEG/lithium acetate yeast transformation kit (Millipore-Sigma, YEAST1). Plasmids with 2XGFP-Atg8 in pRS303 ...
-
bioRxiv - Plant Biology 2021Quote: ... LHCBM9 (M. Schroda, unpublished data), CF1β (Lemaire and Wollman, 1989), RPL1 (Ries et al., 2017) and the HA-tag (Sigma-Aldrich H3663). Anti-rabbit-HRP (Sigma-Aldrich ...
-
bioRxiv - Microbiology 2022Quote: ... full-length InvL with a C-terminal His10 tag was expressed from pET-22b(+)::invL-his10 in Rosetta-gami 2(DE3) cells (Novagen, Madison, WI) using ZYM-5052 autoinducible media for 72 h at 20°C (103) ...
-
bioRxiv - Microbiology 2020Quote: ... acidophilum were PCR-amplified from their respective genomic DNA template and inserted into a pET16b vector containing 10x histidine tag at the N-terminus (Novagen, Madison, WI). The following primers were used ...
-
bioRxiv - Developmental Biology 2021Quote: ... FLAG-tag immunoprecipitation was performed using an α-FLAG antibody (Monoclonal ANTI-FLAG® M2 antibody produced in mouse, Sigma Aldrich, #F3165) and Protein G magnetic beads (Invitrogen™ Dynabeads™ Protein G ...
-
bioRxiv - Microbiology 2023Quote: ... a BiP secretion sequence and a C-terminal C-tag) was expressed from a stable S2 cell line34 (ExpreS2ion Biotechnologies) in EX-CELL® 420 Serum Free Medium (Sigma Aldrich). After 3-4 days ...
-
bioRxiv - Cell Biology 2023Quote: ... was cloned into a pLIC vectors with a TEV-cleavable N-terminal Glutathione S-transferase tag and transformed into Rosetta™ 2 (pLysS) competent cells (Sigma-Aldrich). GST-Cse4END was purified as described above ...
-
bioRxiv - Immunology 2023Quote: ... with addition of Phos-tag Acrylamide (Wako Chemicals, AAL-107) to a final concentration of 30 µM and manganese chloride(II) (Sigma-Aldrich, #63535) to 60 µM ...
-
bioRxiv - Plant Biology 2024Quote: ... and combined with 0.2 mL of a 3 mg L-1 TAG C15:0 solution (Tripentadecanoin, reference T4257, Sigma-Aldrich™, USA) in anhydrous hexane 95 % (296090 ...
-
bioRxiv - Microbiology 2024Quote: Immunoblotting assays were realized in PVDF membranes using Strep-Tag® II monoclonal antibodies (at 1:1000 dilution from a 200 μg/ml solution) (Novagen®) and immunoglobulin G-alkaline phosphatase conjugates (at 1:5000 dilution) ...
-
bioRxiv - Neuroscience 2024Quote: ... the intracellular FLAG-tag inserted on the C-terminal tail was immunostained using a primary monoclonal mouse Anti-FLAG antibody (1:500; Sigma-Aldrich, #F3165) and a secondary anti-mouse IgG2a Alexa 647 antibody (1:500 ...
-
bioRxiv - Neuroscience 2021Quote: ... parental SK-N-SH cells were trypsinized and resuspended in grafting media in presence of 10μg/ml recombinant NXPH1 (rNXPH1) or 100μg/ml BSA (Ctrl; SIGMA #A7906). To test the growth of NB cells depleted from their α-NRXN1+ subpopulation ...
-
bioRxiv - Pathology 2020Quote: ... each recombinant tau isoform was thawed at room temperature and filtered with a 100 kDa spin column filter (Millipore) to remove unwanted tau aggregates ...
-
bioRxiv - Microbiology 2021Quote: ... neoformans cells were added to washed macrophage monolayers at a 1:1 ratio with 10 units (1 unit = 0.1 ng/mL) of recombinant murine IFNγ (Sigma) and 0.3 μg/ml of lipopolysaccharide (Sigma ...
-
bioRxiv - Microbiology 2020Quote: ... Recombinant E1E2 was extracted from cell membranes using 1% NP-9 and purified via sequential Fractogel EMD TMAE (Millipore), Fractogel EMD SO3− (Millipore) ...
-
bioRxiv - Cell Biology 2021Quote: BMDC and BMLC maturation was induced by overnight treatment with 100 ng/ml recombinant murine TNFα (Sigma-Aldrich, USA). In some experiments ...
-
bioRxiv - Cell Biology 2021Quote: ... Rose-John), 20 U/ml recombinant murine GM-CSF (PharmedArtis, Germany) and 10−6 M dexamethasone (Sigma-Aldrich, USA). Progenitor cells were amplified 5 to 11 days before differentiation into DC was induced by supplementing 200 U/ml recombinant murine GM-CSF ...
-
bioRxiv - Biophysics 2022Quote: ... a heparosan trisaccharide amine derivative was extended with recombinant MBP-PmHS1 synthase using UDP-GlcNAc and UDP-GlcA (Sigma) [48] ...
-
bioRxiv - Biophysics 2020Quote: ... beads were removed and recombinant dynein was concentrated in a 100 KDa cut-off filter (Amicon Ultracel, Merck-Millipore) to a final volume of 500 μL ...
-
bioRxiv - Immunology 2022Quote: ... 20 µM DTT and phosSTOP in the presence of recombinant cyclin A2/cyclin-dependent kinase (CDK)1 (Sigma-Aldrich) and 10 mM ATP incubated for 2 h at 30 °C ...
-
bioRxiv - Immunology 2023Quote: ... SRBCs in Alsever’s (TCS Bioscience) were conjugated to recombinant HEL3X (R. Brink) with 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride (Sigma) as previously described104 ...
-
bioRxiv - Neuroscience 2023Quote: Primary rat cortical cultures were treated with recombinant Tat 1-86 (Diatheva, Italy) and with cocaine-HCl (Sigma Chemicals) as described in each Figure Legend ...
-
bioRxiv - Biochemistry 2023Quote: ... Fractions containing recombinant PKAc verified via SDS-PAGE were pooled and concentrated to 250μL using an Amicon concentrator (10K MWCO; Millipore). The concentrated sample was then applied to a preparative-grade Superdex200 column (GE Healthcare ...
-
bioRxiv - Microbiology 2024Quote: Conical HIV-1 CLPs were assembled by incubating purified recombinant CA (3 mg/mL) with 500 μM IP6 (Sigma), 50 mM MES ...
-
bioRxiv - Biochemistry 2024Quote: ... The positive control for the glucosylceramide N-deacylase assay was recombinant Sphingolipid ceramide N-deacylase (SCDase; Sigma-Aldrich, S2563).
-
bioRxiv - Biochemistry 2024Quote: ... The positive control for the glycolipid N-deacylase assay was recombinant Sphingolipid ceramide N-deacylase (SCDase; Sigma-Aldrich, S2563) (76) ...
-
bioRxiv - Microbiology 2020Quote: ... culture media with serum (DMEM, low glucose, from Sigma-Aldrich; 10% HI-FBS; 1% penicillin-streptomycin solution from Sigma-Aldrich; 1% 200mM L-glutamine from Sigma-Aldrich). All assays were performed with cells in passages 4 to 10.
-
bioRxiv - Microbiology 2021Quote: ... from strain BetaCoV/Wuhan/WIV04/2019 was ordered from IDT (Coralville, IA, USA) and cloned into a HIS-SUMO expression vector (a modified pET-DUET; Novagen, Madison, WI, USA). After transformation into BL21(DE3) ...
-
bioRxiv - Cell Biology 2020Quote: ... and subjected to SDS-PAGE followed by Coomassie Blue staining and Western Blot analysis using an anti-His antibody (Sigma-Aldrich, Darmstadt, Germany) or anti-MYC antibodies (Hruby et al. ...
-
bioRxiv - Biophysics 2021Quote: ... Protein production was confirmed by SDS-PAGE and subsequent Western blotting analysis of a K30 sample after 24 h using an anti-His antibody from mouse (dilution 1:3000, Sigma-Aldrich, cat.no. H1029) and secondary anti-mouse IgG-HRP antibody produced in goat (dilution 1:20,000 ...
-
bioRxiv - Microbiology 2022Quote: ... and then transferred to medium differing by the absence of histidine (his) and the presence of 5 mM 3-amino-1,2,4-triazole (3-AT; Sigma-Aldrich, Saint-Louis, MO, USA). Homologous recombination between pPC86 and the PCR product then reconstituted the expression cassette encoding the AD-fused prey protein ...
-
bioRxiv - Cell Biology 2023Quote: ... the membrane was sequentially incubated at room temperature for one hour with anti-His and conjugated anti-mouse antibodies (Sigma-Aldrich, Darmstadt, Germany) (Wu et al. ...
-
bioRxiv - Genetics 2023Quote: ... The samples were collected for a range of incubation times and then examined by western blotting using an anti-His antibody (Sigma, catalogue no. SAB1305538) or anti-GFP (Roche ...
-
bioRxiv - Microbiology 2024Quote: ... Murine bone marrow neutrophils were resuspended in RPMI 1640 medium with no glutamine and no phenol red supplemented with 2% HI-FBS and 1mM Luminol (Millipore Sigma #123072-2.5g) at 1.11x106 neutrophils/ml ...
-
bioRxiv - Biochemistry 2024Quote: ... Membrane was blocked with 1% milk solution and probed with Anti-6 x His Primary mouse antibody (Millipore #05-949, 1:1000 dilution) overnight at +4°C ...
-
bioRxiv - Bioengineering 2024Quote: ... followed by incubation with mouse monoclonal anti-His and biotinylated anti-mouse IgG antibodies (both from Sigma and overlaid at 10 µg/mL). Biotinylated plant lectin ConA (Vector Laboratories ...
-
bioRxiv - Developmental Biology 2021Quote: ... Even protein loading and successful protein transfer was confirmed using Ponceau S stain (Sigma Aldrich) prior to probing with primary antibodies (Table S2) ...
-
bioRxiv - Cell Biology 2022Quote: ... for total protein loading quantification before proteins were transferred to PVDF membrane (Millipore, Cat. # IPVH00010). The blots were incubated with primary antibodies against SUMO2/3 (Enzo Life Sciences ...
-
bioRxiv - Immunology 2021Quote: ... with protein concentrations normalized to total protein levels in lysate (BCA assay; Sigma-Aldrich®).
-
bioRxiv - Cell Biology 2021Quote: ... and the protein concentration was estimated using the Bio-Rad Protein Assay Dye Reagent (Sigma). 10 µl of liver lysate was transferred to 96-well plate and ALT was measured using a microplate-based ALT activity assay kit (Pointe Scientific ...
-
bioRxiv - Cell Biology 2020Quote: Proteins of cellular membrane were extracted using the ProteoExtract® Transmembrane Protein Extraction Kit (NOVAGEN). Total 500 μg of membrane proteins were used for immunoprecipitation with an anti AT1-R or LOX-1 antibody ...