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Citations for Millipore Sigma :
2751 - 2800 of 5488 citations for IL 8 Human since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... Plates were washed with PBS-T and 3,3’,5,5’-tetramethylbenzidine peroxidase substrate (TMB, T0440-IL, Sigma, St Louis, MO, USA) was added ...
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bioRxiv - Microbiology 2022Quote: ... according to the manufacturer’s instructions and activated by incubation in the presence of 100 U/ml IL-2 (Sigma Aldrich) and T Cell TransAct™ human (Miltenyi Biotec ...
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bioRxiv - Immunology 2024Quote: BMDM were polarized or not with recombinant IL-4 (10 ng mL−1, peprotech) for 24 hours and with LPS (10 ng/ml, Sigma) for 8 hours ...
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bioRxiv - Cancer Biology 2022Quote: ... were cultured in the presence of recombinant mouse IL-4 (25 ng/mL; #404-ML, R&D) and LPS (20 μg/mL; #L2880, Sigma) over night to induce B cell activation and terminal differentiation ...
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bioRxiv - Genetics 2022Quote: ... cells were grown in a basal erythroid medium (BEM) supplemented with hSCF, IL-3, EPO (Eprex, Janssen-Cilag) and hydrocortisone (Sigma). From day 6 to 20 ...
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bioRxiv - Immunology 2023Quote: ... Sorted Treg cultures also contained 1000 U/mL of IL-2 (Proleukin) and 50 nmol/L of rapamycin (Sigma- Aldrich), whereas Tconv cultures contained 100 U/mL of IL-2 ...
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bioRxiv - Immunology 2023Quote: ... ex vivo isolated naïve or overnight stimulated (anti-CD40 at 5μg/ml and IL-4 at 1ng/ml) 3×105 B cells were plated on a poly-D lysine (PDL) (Sigma)-coated XF96 cell culture microplate and incubated at 37 °C for a minimum of 30 min in a CO2-free incubator in assay medium (XF RPMI medium pH 7.40 supplemented with 2 mM L-glutamine ...
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bioRxiv - Neuroscience 2023Quote: ... and reconstituted to 1 mg/mL in sterile water and diluted to 250 μg/mL (IL-9) or 10 μg/mL (IFNγ and IP-10) in 0.1% cell-culture grade bovine serum albumin (Sigma A9418) in 1X PBS ...
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bioRxiv - Cancer Biology 2023Quote: ... 3 and 7 post IL-12mRNA/scRNA injection (day 11, 13 and 17 post inoculation) and digested in 30% collagenase (Sigma) for 30 minutes at 37°C ...
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bioRxiv - Bioengineering 2024Quote: ... the hydrogel- containing cells were maintained in RoosterBasal™ medium and treated with 1 ng/mL of IL-1β (Sigma) for 4 days at 37 °C and 5% CO2 ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were cultured in 50µL of IL-11 and SCF media2 for 2h before being supplemented with polybrene (Sigma-Aldrich) and up to 20uL of a lentivirus equipped with the gene of interest (STAT5B-WT or STAT5B-Y699F ...
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mTOR inhibition enhances delivery and activity of antisense oligonucleotides in uveal melanoma cellsbioRxiv - Cancer Biology 2021Quote: ... UM cells were transduced with virus supplemented with 8 µg/ml polybrene (Sigma Aldrich, Saint Louis, Missouri, USA) and 24 h after transduction ...
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bioRxiv - Cell Biology 2020Quote: ... McArdle-RH7777 or IMR-90 were then transduced with the virus with 8 µg/ml polybrene (Sigma-Aldrich). After 24 h ...
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bioRxiv - Genetics 2021Quote: A stock dye solution containing 5 mg/μl red fluorescent lipophilic dye DiI (Sigma, Cat #41085-99-8) was diluted in M9 buffer by 10,000 times for optimal signal intensity ...
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bioRxiv - Cell Biology 2022Quote: ... diluted with fresh medium (1/2) and hexadimethrine bromide was added (final concentration 8 μg/ml; Sigma-Aldrich). Target cells were then infected ...
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bioRxiv - Molecular Biology 2020Quote: ... twice with LiCl wash buffer (10 mM Tris-HCl [pH 8], 250 mM LiCl, 0.5% sodium deoxycholate, 1 mM EDTA, 0.5% IGEPAL CA-630 [Sigma]), and twice with 10 mM Tris-HCl [pH 8] ...
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bioRxiv - Molecular Biology 2020Quote: ... Target cells were infected for 48 hr with lentiviral supernatants supplemented with 8 µg/ml polybrene (Sigma-Aldrich). Following a 24-hr recovery in fresh media ...
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bioRxiv - Microbiology 2020Quote: ... in THF (tetrahydrofurane) and 2 µL 200 mM ATPS (8-aminopyrene-1,3,6-trisulfonic acid trisodium salt, Sigma-Aldrich) in 15% acetic acid (v/v) ...
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bioRxiv - Physiology 2019Quote: Chemotaxis of hMSCs was assessed using Boyden chambers with a pore size of 8 μm (Merck Millipore, PIEP12R48). Cells were seeded on the upper membrane in serum free α-MEM medium at a density of 30,000 cells/cm2 and allowed to adhere 4 h before being transferred to the wells containing chemotactant (Medium (serum free) ...
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bioRxiv - Immunology 2019Quote: ... Viral particles were added to cultured THP-1 cells in R10 together with 8 μg/ml Polybrene (Sigma) to improve transfection efficiency ...
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bioRxiv - Microbiology 2019Quote: ... and forced through a 25-mm diameter Whatman Nuclepore Track-Etched Membrane with 8-µm holes (Sigma-Aldrich). The cellular debris ...
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bioRxiv - Neuroscience 2020Quote: Replicates of hiPSC-derived neurons were harvested with lysis buffer (8 M Urea, 50 mM ammonium bicarbonate (Sigma), EDTA-free protease inhibitor Cocktail (Roche) ...
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bioRxiv - Cell Biology 2019Quote: ... Concentrated lentivirus was diluted with growth medium and mixed 1:1 with polybrene (8 μg/ml; EMD Millipore) in Opti-MEM (Gibco) ...
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bioRxiv - Bioengineering 2021Quote: SMCs derived from hiPSCs were suspended (2.5×105 cells) in 60μl of an 8 mg/ml fibrinogen solution (Sigma). Hence ...
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bioRxiv - Biochemistry 2020Quote: ... we intraperitoneally (i.p.) administrated the AMPA/kainate receptor agonist kainate (8 mg/kg; Sigma, St. Louis, MO, USA) dissolved in 0.1 M phosphate buffered saline (PBS ...
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bioRxiv - Neuroscience 2021Quote: ... cells were lysed by scraping in a total volume of 80 µL of lysis buffer (containing 50 mM Tris-HCl pH 8, 150 mM NaCl, 0.5% sodium deoxycholate, 0.5% Triton-X 100, Sigma) and processed for immunoblotting as described in this manuscript under the PUM1 validation subchapter ...
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bioRxiv - Neuroscience 2021Quote: ... cells were scraped with 100 µL lysis buffer (50 mM Tris-HCl pH 8, 150 mM NaCl, 0.5% sodium deoxycholate, 0.5% Triton-X 100, Sigma) supplemented with EDTA-FREE cOmplete Mini protease inhibitor cocktail (Sigma) ...
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bioRxiv - Neuroscience 2020Quote: ... Periorbital thresholds were assessed hourly for 8 hours following ethanol (300 mg/kg i.p. Sigma, St Louis, MO) administration as previously performed (Maxwell et al. ...
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bioRxiv - Plant Biology 2020Quote: ... Protein extracts were prepared in two volumes (approx.8 ml) of CelLyticP buffer (Sigma-Aldrich, Cat No C2360) with 1 % of a Protease Inhibitor EDTA-free tablet (Roche ...
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bioRxiv - Microbiology 2021Quote: ... followed by pH 8-buffered 25:24:1 phenol:chloroform:isomyl alcohol and 24:1 chloroform:isomyl alcohol (Sigma-Aldrich®). Extracts were concentrated using 100 kDa membrane centrifugation (Amicon®) ...
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bioRxiv - Microbiology 2021Quote: ... sliced into cryosections (8-μm-thick) and stained with Oil Red O (Sigma-Aldrich, St. Louis, MO, USA) to assess the fatty droplet content ...
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bioRxiv - Microbiology 2021Quote: ... 8-10 seeds were plated on pre-dried 1% MS-agar (Murashige and Skoog basal salts mixture; Sigma) (2.2 g l−1 ...
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bioRxiv - Microbiology 2021Quote: ... were transduced with the lentiviruses at 0.3 infectious units/cell diluted in 8 μg/ml polybrene (EMD Millipore) as previously described ...
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bioRxiv - Microbiology 2021Quote: ... were transduced with the lentivirus at 0.3 infectious units/cell diluted in 8 μg/ml polybrene (EMD Millipore) as previously described ...
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bioRxiv - Microbiology 2021Quote: ... 4T1 cells were incubated in the filtrated viral supernatant in the presence of 8 μg/ml polybrene (Sigma) for 24 h ...
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bioRxiv - Cancer Biology 2021Quote: ... The migration ability of RAW 264.7 cells was examined using indirect transwell cell culture system (8 μm, Millipore). 4 × 105 of ID8 cells were seeded in 6-well plates evenly ...
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bioRxiv - Cell Biology 2021Quote: ... supplemented with 8% fetal calf serum (FBS; CapriCorn Scientific) and 100 U/ml of penicillin/streptomycin (Sigma-Aldrich). All cell cultures were maintained at 37°C ...
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bioRxiv - Cell Biology 2020Quote: ... 8-week-old mice were anesthetized by isofluorane (CDMV) inhalation and 50 μl of 10 μM ctx (Sigma) was injected into the TA muscle ...
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bioRxiv - Cell Biology 2021Quote: ... and used to infect HeLa cells in the presence of 8 μg/ml hexadimethrine bromide (Sigma-Aldrich, #H9268). After 24 h ...
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bioRxiv - Cell Biology 2021Quote: ... Adherent HEMs or MNT1 cells were incubated with viral particles and 8 μg/ml polybrene (H9268, Sigma-Aldrich) for 24 h ...
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bioRxiv - Cancer Biology 2020Quote: ... Equal amounts of protein were separated by 8-12% SDS-PAGE and transferred to PVDF membranes (Millipore-Merck). Membranes were incubated with primary antibodies ...
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bioRxiv - Cell Biology 2021Quote: ... Free Biotin-14-dCTP was removed by extensive buffer exchange with TE buffer (10 mM Tris.HCl pH 8, 0.1 mM EDTA) in a 100 kDa Amicon Ultra spin concentrator (Millipore). The biotinylated DNA was then concentrated to ∼10 mg/ml and stored at −20 °C until needed.
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bioRxiv - Microbiology 2022Quote: ... 16HBE cells were grown on 8-well glass slides (Nunc Lab-Tek II chamber slide system; Sigma-Aldrich) with each well seeded with 450 µl of cell suspension at 1.2 x 105 cells/ml and grown for 24 hours ...
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bioRxiv - Neuroscience 2022Quote: ... the sections were incubated in Tris-buffer (0.5 M, pH 8; TB) containing 0.025% diaminobenzidine solution (DAB wt/vol, Sigma) and 0.5% nickel ammonium sulfate (wt/vol ...
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bioRxiv - Molecular Biology 2022Quote: ... 30 µg retina protein extracts were separated on 8% polyacrylamide gels and blotted onto PVDF transfer membranes (Millipore) followed by blocking with non-fat dried milk (Applichem) ...
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bioRxiv - Molecular Biology 2022Quote: ... 8 M urea and transferred onto a 30 kDa molecular weight cut-off column (Microcon-30, Merck Millipore). 150 μL 100 mM TRIS/HCl pH 8.5 ...
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bioRxiv - Molecular Biology 2022Quote: EBIV were first concentrated by adding 3.2 g polyethylene glycol (PEG) 8000 (8%, W/V, MilliporePEG8000, Sigma, USA) and 0.9 g NaCl (0.3 mol/L ...
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bioRxiv - Cell Biology 2022Quote: ... RPE1 cells were infected with Lentiviral packaged particles by supplementing 8 μg/ml protamine sulfate (P4505; Sigma-Aldrich), the virus infected cells were selected by overnight puromycin (7 μg/ml ...
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bioRxiv - Cell Biology 2022Quote: ... were transduced with inducible STEMCCA and rtTA lentivirus–containing supernatants overnight in polybrene (8 μg/ml; Sigma-Aldrich). Cells were expanded and transduced with pLKO viruses expressing control shRNA or shRNAs targeting SOX15 ...
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bioRxiv - Cell Biology 2022Quote: ... Hexa KO cells were treated with the retrovirus containing medium and 8 μg/mL polybrene (Sigma-Aldrich, H9268). After 2 days ...