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Citations for Millipore Sigma :
2751 - 2800 of 10000+ citations for Biotin Z Antibody Internalization Kit mouse since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2021Quote: ... rabbit polyclonal antibody directed against Iba-1 (FUJIFILM Wako, dilution 1:500) and mouse monoclonal antibody directed against GAD67 (Millipore Sigma, dilution 1:500). For the secondary antibody ...
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bioRxiv - Systems Biology 2020Quote: ... Cells were stained for bait proteins using mouse anti-FLAG antibody (Monoclonal anti-FLAG M2 antibody, Sigma-Aldrich, Cat# F3165; used at 1:2500), Rabbit-anti-Giantin antibody (Abcam ...
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bioRxiv - Cancer Biology 2022Quote: ... and rabbit anti-pSer502-NT5C2 (dilution 1:1000) (Covance) antibodies and β-actin with a mouse monoclonal anti-β-actin antibody (Sigma Aldrich, #A5441). We visualized the immunoblots using an Odyssey Infrared Imaging System (LI-COR Biosciences) ...
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bioRxiv - Plant Biology 2022Quote: ... and αTubulin levels were used as loading control (α_Tubulin antibody, Monoclonal Anti-α-Tubulin antibody produced in mouse, Sigma, Catalog number T5168; anti-Rabbit IgG, Sigma, Catalog number A0545).
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bioRxiv - Molecular Biology 2022Quote: ... Endogenously expressed AGAL and tubulin were detected with rabbit monoclonal anti-galactosidase alpha antibody (used for staining of AGAL in kidney) and mouse monoclonal anti-acetylated tubulin antibody (T7451, SIGMA-Aldrich, Prague, Czech Republic), respectively.
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bioRxiv - Cell Biology 2024Quote: ... The primary antibodies were detected by incubation with horseradish peroxidase (HRP)-conjugated anti-mouse or anti-rabbit secondary antibodies (Sigma-Aldrich, A9044 and A5278), followed by incubation with the Clarity Western ECL Substrate (Bio-Rad ...
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bioRxiv - Microbiology 2024Quote: ... tissue sections were exposed to anti-Iba1 rabbit antibody (FUJIFILM Wacko, 013-27691, 1:300 dilution) and anti-GFAP mouse antibody (Sigma, G3893, 1:300 dilution) for overnight at room temperature ...
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bioRxiv - Genetics 2023Quote: ... The presence of the tagged protein was identified by immunoblotting using anti-FLAG antibody (F1804 monoclonal antibody, clone M2 in mouse, SLCK5688, Sigma Aldrich, Supplemental Fig 2F). Rescue was confirmed by measuring cilia length ...
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Retinal affectation in Huntington’s disease mouse models concurs with a local innate immune responsebioRxiv - Neuroscience 2023Quote: ... primary antibody against α-tubulin (T5168) and secondary antibodies (A0545, anti-rabbit; A9044, anti-mouse IgG-peroxidase) were purchased from Sigma-Aldrich (Darmstadt, Germany).
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bioRxiv - Cancer Biology 2022Quote: ... The membrane was washed and incubated with primary and secondary antibodies following the procedure described above but using the following antibodies: mouse α-FLAG (Sigma #F3165, 1:500) and donkey α-mouse HRP (Invitrogen #A16017 ...
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bioRxiv - Developmental Biology 2024Quote: ... Embryos were blocked with 5% BSA in 1X PBS containing 0.1% Tween-20 (PBT) at RT for 1 hour and then incubated with primary antibody solution containing anti-chondroitin sulfate antibody (Millipore, monoclonal, mouse, 1:100) and biotinylated-Hyaluronan Binding Protein (Millipore ...
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bioRxiv - Microbiology 2021Quote: ... Insulin was measured using the rat/mouse insulin ELISA kit from Millipore (EZRMI-13K), according to the manufacturer’s instructions (Wang et al. ...
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bioRxiv - Biochemistry 2020Quote: ... The mAbs were isotyped using a mouse mAb isotyping test kit (Sigma-Aldrich, USA). The icELISA described above was used to evaluate the affinity (Ka ...
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bioRxiv - Molecular Biology 2021Quote: ... Serum TSH was analyzed with a mouse pituitary magnetic bead panel kit (EMD Millipore Corp. ...
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bioRxiv - Cancer Biology 2021Quote: PLA experiments were performed using the Red Mouse/Rabbit Duolink Starter Kit (Sigma-Aldrich) according to the manufacturer’s instructions ...
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Vitamin D signaling orchestrates skeletal muscle metabolic flexibility by regulating its fuel choicebioRxiv - Physiology 2023Quote: ... Serum insulin levels were determined by ELISA using mouse insulin ELISA kit from Sigma according to the manufacturer’s instructions.
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bioRxiv - Developmental Biology 2022Quote: GH was measured using a mouse/rat growth hormone ELISA kit (Millipore – EZRMGH-45K), based on quantitative sandwich enzyme immunoassay technique ...
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bioRxiv - Cell Biology 2023Quote: The Duolink In Situ Red Starter Mouse/Rabbit kit was from Sigma (#Cat DUO92101) and used according to the manufacturer’s protocol with primary antibodies to Orai1 (mouse 1:500 ...
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bioRxiv - Microbiology 2022Quote: ... were resuspended in 100 μL of FC buffer containing primary antibodies (mouse anti-Spike S2, Thermo Fisher, Cat #: MA5-35946, 1:500 dilution) (mouse anti-Flag M2, Sigma, Cat #: F1804, 1: 500 dilution), or anti-SARS-CoV-2 human sera (1:100 dilution ...
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bioRxiv - Developmental Biology 2021Quote: Recombination of Col1a2xLPCherry-Tg was induced by keeping animals in separated tanks with water containing (Z)-4-hydroxytamoxifen (4-OHT, Sigma T5648) 2 µM overnight ...
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bioRxiv - Cancer Biology 2020Quote: ... solution (Cat no. H1009), AZD2461 (Cat no. SML 1858) and MG132 (Z-Leu-Leu-Leu-al, Cat no. C2211) were from Sigma Aldrich.
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bioRxiv - Synthetic Biology 2022Quote: ... The identification of amino acids was based on retention times and m/z values obtained from amino acid standards (Sigma-Aldrich)
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bioRxiv - Molecular Biology 2022Quote: ... 34 mg/mL chloramphenicol) and 2 μL DMSO containing 100x desired final concentration of 5-aminoimidazole-4-carboxyamide-ribonucleoside (Z) (Millipore Sigma) in 2 mL 96-well culture blocks ...
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bioRxiv - Cell Biology 2023Quote: ... Genetical labelling was induced in epidermis of 6-8 weeks old mice with a single topical administration of 75 μg of (Z)-4-Hydroxytamoxifen (Sigma-Aldrich) (15 mg/mL diluted in acetone).
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bioRxiv - Neuroscience 2024Quote: ... In Aldh1l1-CreER;mGluR5fl/fl;GCaMP6s and Aldh1l1-CreER;mGluR3fl/fl;GCaMP6s mice (Z)-4-Hydroxytamoxifen (4-OHT; H7904; Sigma-Aldrich) was used ...
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bioRxiv - Molecular Biology 2020Quote: ... The primary and secondary antibodies used for detecting the 3×Flag were monoclonal ANTI-FLAG® M2 antibody produced in mouse (Sigma-Aldrich, Cat NO.: F3165) and Goat Anti-Mouse IgG (H + L)-HRP Conjugate (Bio-Rad ...
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bioRxiv - Biochemistry 2020Quote: ... blots were incubated for 1 hour rolling at RT in secondary antibody which was anti-mouse antibody raised in sheep (Sigma-Aldrich, St. Louis, MO, USA) diluted 1:10,000 ...
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bioRxiv - Systems Biology 2021Quote: ... for 1.5 hours at room temperature before being stained for bait protein with mouse anti-FLAG antibody (Monoclonal anti-FLAG M2 antibody; Sigma-Aldrich, Cat# F3165; 1:500) in blocking buffer overnight at 4℃ ...
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bioRxiv - Neuroscience 2021Quote: ... neurons were concomitantly labelled with two antibodies: one for neurite labelling (mouse anti-Neurofilament 160/200 monoclonal antibody (Sigma-Aldrich N2912, 1:10000-1:20000) and the other for neuronal nuclei (mouse anti-NeuN monoclonal antibody (Merck MAB377 ...
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bioRxiv - Systems Biology 2023Quote: ... well-validated custom and commercial primary antibodies against proteins of interest were carefully selected and experimental parameters rigorously optimized to ensure high fidelity labeling: Connexin 43 (Cx43; mouse monoclonal antibody, Cat. # MAB3067, EMD Millipore Corp., Darmstadt, Germany; rabbit polyclonal antibody, Cat. # C6219, Millipore Sigma, St. Louis, MO), N-cadherin (N-cad ...
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bioRxiv - Cell Biology 2024Quote: Following primary antibodies for immunofluorescence (IF) and western blotting (WB) with indicated dilution were used: mouse anti-γ tubulin antibody (Sigma #T5326, 1:100 IF), rabbit anti-Centrin-1 antibody (Abcam#ab101332 ...
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bioRxiv - Neuroscience 2024Quote: ... then incubated with primary antibodies for 30 minutes at room temperature: mouse anti-NeuN antibody conjugated to Alexa 488 (Sigma Aldrich, Cat# MAB377X, 1:100), rabbit anti-TMEM119 antibody (Sigma Aldrich ...
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bioRxiv - Molecular Biology 2021Quote: ... 1/1000) antibodies for 2 h at 37 °C in antibody diluent solution (Duolink in situ kit, Sigma-Aldrich). The cells were then labeled with the PLA antimouse PLUS probe (DUO92001 ...
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bioRxiv - Cancer Biology 2021Quote: ... and bounded proteins were eluted with NETN buffer containing 2 mg/ml biotin (Sigma-Aldrich) for 1 h twice at 4 °C ...
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bioRxiv - Molecular Biology 2020Quote: ... a 1 mg ml−1 PBS solution of biotin-labelled bovine serum albumin (A8549, Sigma) was applied to the chambers for 5 minutes and then washed with excess PBS ...
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bioRxiv - Molecular Biology 2019Quote: Selected oligonucleotides were ordered with a 5′-biotin tag and HPLC-purified from Sigma-Aldrich. Each oligo was diluted to 100 μM in nuclease-free 10 mM Tris pH 8 (diluted from Ambion 1 M Tris pH 8 ...
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bioRxiv - Cell Biology 2020Quote: ... growth media was replaced with complete growth media supplemented with 50 μM biotin (Sigma-Aldrich) and cultured for a further for 16 hours ...
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bioRxiv - Neuroscience 2019Quote: ... then incubated with biotin-conjugated Wisteria Floribunda Agglutinin Lectin (labels PNNs; 1:500; Sigma-Aldrich) overnight at RT in a 5% normal goat serum/0.25% Triton-X solution ...
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bioRxiv - Cell Biology 2021Quote: ... Biotin-647-microtubules were attached to this surface with streptavidin (0.625 mg/ml) (#S4762 Sigma) and the surface was blocked with κ-casein (1 mg/ml ...
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bioRxiv - Biophysics 2020Quote: ... The pellet was then resuspended in conjugation buffer containing 1 mM Biotin-NHS (Sigma 203118) by pipetting up and down ...
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bioRxiv - Cell Biology 2021Quote: ... Then the cultures were grown in culture media supplemented with 150 μM biotin (Sigma-Aldrich) and compound 2 was added for 8 hours ...
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bioRxiv - Neuroscience 2022Quote: ... Sections were then incubated in biotin-conjugated Wisteria floribunda agglutinin (WFA, 1:1000, Sigma-Aldrich), mouse anti-PV (1:500 ...
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bioRxiv - Bioengineering 2022Quote: ... Excess biotin was removed with a 5K MWCO concentrator (CLS431487-12EA, Millipore Sigma, MA, USA) at 4000xg for 100 minutes at 4°C ...
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bioRxiv - Neuroscience 2019Quote: ... 1:200) and lectin for blood vessels (Biotin-agglutinin lectin Ulex europaeus, Sigma L8262,1:1000) and were applied overnight in 1% NDS ...
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bioRxiv - Bioengineering 2020Quote: ... The eluted His-tagged RBD was then incubated with biotin-tagged HRV C3 protease (Sigma) and passed through a streptavidin-agarose column to deplete the protease and the 8XHis-SBP peptide ...
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bioRxiv - Cell Biology 2019Quote: ... cultures were incubated for 24 hours in media containing 2.5nM WR + 150μg of biotin (Sigma). 48 mL of asynchronous culture at high parasitemia (10% or higher ...
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bioRxiv - Biochemistry 2021Quote: ... the media were replaced with fresh media containing 50 μM D-biotin (Sigma B-4501). After 16 hours of labeling ...
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bioRxiv - Biophysics 2021Quote: ... Excess mSA molecules were removed by passing the sample through biotin–conjugated agarose resin (Sigma). Free thiols on GlpG were alkylated by iodoacetamide (10 mM) ...
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bioRxiv - Cell Biology 2020Quote: ... Growth media was replaced with complete growth media supplemented with 50 μM biotin (Sigma-Aldrich), and cells cultured for a further 16 hours ...
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bioRxiv - Neuroscience 2022Quote: ... a solution of biotin-conjugated lectin Wisteria floribunda (WFA) (10 µg/ml; Sigma-Aldrich, L1516) was added in the primary antibody solution.