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Citations for Millipore Sigma :
2751 - 2800 of 4275 citations for 6 TERT BUTYLQUINOLINE 98.0+% GC T since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... Membranes were washed a further three times in TBS-T prior to antibody detection using enhanced chemiluminescence horseradish peroxidase substrate detection kit (Millipore, Hertfordshire, UK). Imaging was undertaken using a G:BOX Chemi-XR5 (Syngene ...
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bioRxiv - Microbiology 2021Quote: ... sacculi were centrifuged and redissolved in 20 ml of Tris-HCl buffer (50 mM, pH 7.6) with 2 mg Trypsin (Sigma-Aldrich, T-4799). Trypsin digestion was performed overnight at 37 °C under constant shaking ...
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bioRxiv - Bioengineering 2020Quote: ... T cells were stimulated by adding 100 μl of supplemented EMEM with 40 μg/ml phytohemagglutinin (PHA; Sigma-Aldrich, St. Louis, MO) to each well containing MSCs and PBMCs ...
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bioRxiv - Immunology 2022Quote: ... The plates were then washed five times in PBS-T and blocked with buffer containing 5% milk (Difco #232100) and 1% BSA (Sigma #A906-500G) in PBS-T (BioVision #2310-100) ...
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bioRxiv - Biochemistry 2022Quote: ... Membranes were blocked with 5% milk in Tris-buffered saline with 0.1% Tween-20 (TBS-T) and incubated at 4°C overnight with the following antibodies: anti-FLAG (Sigma, F1804; 1:1,000), anti-SLC1A3 (Genetex ...
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bioRxiv - Neuroscience 2021Quote: ... with 0.05% Tween 20 (TBS-T) and then incubated with the appropriate primary antibody overnight in 5% BSA (Sigma-Aldrich, MO, USA) in TBS-T at 4°C ...
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bioRxiv - Neuroscience 2021Quote: ... membranes were washed three times with TBS-T and developed in a chemiluminescence system (Fusion FX Vilber Lourmat, Vilber, France) using chemiluminescent HRP substrate (Millipore, MA, USA). Intensities of specific bands were normalized to a protein loading control or to the total protein levels marked using MemCode™ Reversible Protein (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2022Quote: ... Membranes were washed three more times in TBS-T before antibody detection using enhanced chemiluminescence horseradish peroxidase substrate detection kit (Millipore, Hertfordshire, UK). Imaging was conducted using a G:Box Chemi-XR5 (Syngene ...
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bioRxiv - Microbiology 2022Quote: ... Membranes were blocked with 5% bovine serum albumin in tris-buffered saline/0.1% [vol/vol] tween-20 (TBS-T) before probing overnight at 4°C with antibodies raised to the following targets: mouse anti-puromycin (Millipore-Sigma, MABE343), mouse anti-OC43 N (CoV antibody ...
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bioRxiv - Microbiology 2022Quote: ... Membranes were blocked in 5% milk in TBS-T followed by incubation in the following primary antibodies that were diluted in TBS-T containing 5% milk: rat-anti-HA 3F10 (Sigma, 1:1,000) and rabbit-anti-aldolase (14 ...
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bioRxiv - Microbiology 2022Quote: ... Membranes were blocked with 5% bovine serum albumin (BSA) in PBS-T (0.05% Tween 20) and subsequently probed with the rat anti-HA mAb 3F10 (Sigma, 1:1,000 dilution), followed by biotin-conjugated anti-rat antibody (Roche ...
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bioRxiv - Microbiology 2019Quote: ... The beads were resuspended in 100 μl of NP‐T buffer containing 1 mM MgCl2 and 50 units of Benzonase (Sigma; cat # E1014) and incubated for 10 min at 37°C in a heat block ...
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bioRxiv - Developmental Biology 2020Quote: ... washed with TBS-T and incubated overnight with primary antibodies (anti-Actin 1:10000 Santa Cruz Biotechnology #sc1616; anti-Dab1 1:2000 Sigma-Aldrich #HPA052033 ...
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bioRxiv - Pathology 2019Quote: ... The membranes were then washed in PBS-T and incubated overnight at 4°C in PBS-T containing 5% milk powder and primary antibody: monoclonal anti-GFAP (Sigma-Aldrich, G3893) in dilution 1:400 or monoclonal anti-actin (MP Biomedicals ...
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bioRxiv - Pathology 2019Quote: ... membranes were incubated for 30 min at room temperature in PBS-T containing 5% milk powder and secondary antibody: peroxidase-conjugated anti-mouse IgG (1:5000) (Sigma-Aldrich, A2304). The membranes were then washed in PBS-T ...
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bioRxiv - Microbiology 2019Quote: ... plates were incubated with horseradish peroxidase-labelled goat anti-mouse IgG antibody (Sigma-Aldrich, cat # AP124P, diluted 1:3000 in PBS-T) for 1 h ...
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bioRxiv - Developmental Biology 2019Quote: ... the membranes were washed 3x in TBS-T for 5 min each and incubated for 1 h with peroxidase-conjugated anti-rabbit secondary antibody (Sigma, cat # A0545) diluted 1:10,000 in TBS-T + 1% BSA solution ...
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bioRxiv - Physiology 2020Quote: ... total volume 100 μL) without (control) or with T-lowering SRB (target 10 nM in plasma; 341738, Sigma Aldrich, St. Louis, MO). In the injections for both control and experimental groups ...
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bioRxiv - Microbiology 2021Quote: ... The membranes were washed three times with PBS-T at 4°C and the specific proteins were visualized with a chemiluminescence HRP substrate system (Millipore, Massachusetts, USA) using LAS 4000 (Fujifilm Life Science ...
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bioRxiv - Cancer Biology 2021Quote: ... After further washes with TBS-T, detection was performed using EZ-ECL Chemiluminescence Substrate (Biological Industries, Israel) or Luminata Forte Western HRP Substrate (Millipore Sigma, MA). Membranes were imaged on Biospectrum 500 (UVP ...
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bioRxiv - Genetics 2020Quote: Wildtype and mutant COCH-expressing FLp-inTM T-RExTM 293 cells were cultured in DMEM-AQ medium (Sigma Aldrich, Saint Louis, USA) supplemented with 10% Fetal Calf Serum ...
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bioRxiv - Animal Behavior and Cognition 2020Quote: ... a standard curve was prepared using a 2-fold serial dilution of Recombinant mouse Prolactin (mPRL; AFP-405C, NIDDK-NHPP) in PBS-T with BSA 0.2 mg/mL (bovine serum albumin; Millipore 82–045–1). After the blocking step ...
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bioRxiv - Immunology 2020Quote: Frozen tissues were homogenized in 10 volumes (mg : µl) of T-PER tissue protein extraction reagent (Thermo, Cat#78510) containing cocktail inhibitor (Sigma, Cat#P8340) and PMSF ...
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bioRxiv - Microbiology 2020Quote: ... The beads were resuspended in 100 μl of NP‐T buffer containing 1 mM MgCl2 and 50 units of Benzonase (Sigma; cat # E1014) and incubated for 10 min at 37°C in a heat block ...
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bioRxiv - Cell Biology 2021Quote: ... Primary antibodies (diluted in 5% BSA in 1X PBS-T) were as follows: Mouse anti-β-Actin (1:10,000, Sigma-Aldrich, A5441), Rat anti-α Tubulin (1:2000 ...
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bioRxiv - Microbiology 2021Quote: ... Positive protein-protein interactions were subjected to another round of stringent selection by testing the yeast transformant’s ability to express α-galactosidase from the MEL1 reporter gene (blue/white screening) when selected on SD/-T/-L/-H agar plates supplemented with X-α-Gal (Sigma, USA) at 30°C.
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bioRxiv - Cell Biology 2022Quote: ... and the regenerated lobe at sacrifice (T=48) with TRI reagent solution according to the manufacturer’s protocol (Sigma-Aldrich, St. Louis, Missouri). The concentration and purity of RNA in the samples were determined by measuring absorbance with the NanoDrop 1000A spectrophotometer (Thermo Scientific) ...
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bioRxiv - Plant Biology 2022Quote: We used the two-tailed Student’s t test (Microsoft Excel 10.0) and ANOVA analysis (Holm and Sidak test; Sigma Plot 11; Systat Software) for multiple genotypes for comparisons ...
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bioRxiv - Microbiology 2022Quote: ... The cells were activated with a cocktail of T-cell activators consisting of 40 ng/ml tumor necrosis factor-alpha (TNF-α; Catalog no. T0157; Sigma-Aldrich), 40 ng/ml phorbol myristate acetate (PMA ...
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bioRxiv - Neuroscience 2024Quote: ... cells from one T-25 flask were dissociated with 2 mL of dissociation reagent containing papain (50U; Sigma-Aldrich; Cat. No. P4762) and Accutase (Thermo-Fischer Scientific ...
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bioRxiv - Microbiology 2024Quote: ... Unbound antibody was removed by 3 x 2-hour washes with PBS-T and the cells were pre-incubated with a final concentration of 500 μM biotin-XX-tyramide (Sigma-Aldrich, # SML3484) at RT ...
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bioRxiv - Neuroscience 2024Quote: ... Membranes were washed five times for 10 minutes each in TBS-T and detected with Immobilon Western Chemiluminescent HRP Substrate (Millipore Sigma, WBKLS0500) on Pro Signal Blotting film (Genesee Scientific ...
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bioRxiv - Neuroscience 2023Quote: ... Samples were washed with FACS buffer (2% fetal bovine serum in PBS) and permeabilized using 0.1% Triton X-100 (Sigma-Aldrich, T-8787) with 1% goat or donkey serum in PBS buffer and blocked with 4% donkey serum for one hour ...
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bioRxiv - Neuroscience 2023Quote: ... Membranes were blocked in 5% BSA TBS-T or 5% skim milk TBS-T and incubated overnight at 4°C with the following primary antibodies diluted in 5 % BSA or 5% skim milk dissolved in TBS-T: pSer293-PDH (1:1,000) (Merck Millipore, Cat. no. ABS204), PDH (1:1,000 ...
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bioRxiv - Microbiology 2023Quote: ... Jurkat Timer clones were treated for 24 h with a combination of T-cell stimulants: phorbol 12-myristate 13-acetate (SIGMA, Cat# P1585) at a final concentration of 20 ng/ml and ionomycin (Nacalai Tesque ...
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bioRxiv - Microbiology 2023Quote: ... The membrane was blocked with PBS + 0.1% Tween 20 (PBS-T) and 5% nonfat milk prior to incubating in monoclonal anti-FLAG M2 antibody (1:40,000, Sigma, Thermo Scientific™). overnight at 4 °C followed by Goat-anti Mouse IgG-HRP secondary antibody (Invitrogen™ ...
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bioRxiv - Immunology 2023Quote: ... Immune mediators in cell culture supernatant following CD3/CD28 bead stimulation were measured using a human premixed CD8+ T Cell Human 17-plex panel (Millipore, Temecula, California). All Luminex assays were analyzed using a MAGPIX Instrument and xPONENT software (Luminex ...
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bioRxiv - Cell Biology 2023Quote: ... Then samples were rinsed twice in 1✕PBS without Ca2+ and Mg2+ for 10 minutes each cycle, then quenched in cold (i.e., t = 4°C) cytoskeletal buffer (i.e., 10 mM MES (Sigma-Aldrich, Cat#M3671-50G), 150 mM NaCl (Flinn Scientific ...
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bioRxiv - Cancer Biology 2023Quote: ... aCD3/CD28 beads were magnetically removed and T cells were resuspended in X-VIVO 15 with 5 µg/mL polybrene (#TR-1003-G, Sigma-Aldrich, USA) at a density of 3 × 106 cells per mL ...
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bioRxiv - Immunology 2023Quote: ... Membranes were again washed with TBS-T for 3 times and were incubated with Immobilon Forte Western HRP substrate (WBLUF0500, EMD Millipore Corporation). Images were captured using AI600 Imager from GE Healthcare Life Science.
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bioRxiv - Neuroscience 2024Quote: ... Sections were washed in TBS-T and incubated overnight at 4 °C in the same buffer with anti-TH monoclonal antibodies (1:500; Millipore, Stockholm, Sweden). Immunoreactivity was detected using a secondary antibody conjugated with Alexa fluorescent dye (1:400 ...
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bioRxiv - Microbiology 2020Quote: ... cells were fixed with formaldehyde/PBS solution (6%) and stained with crystal violet (1%; Sigma Chemical Corp.) in 70% methanol ...
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bioRxiv - Biophysics 2021Quote: ... PS-CI was reduced or oxidised with substoichiometric amounts of sodium dithionite or K3Fe(CN)6 (Sigma) under anaerobic conditions in an electrochemical glass cell equipped with a 4°C water bath ...
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bioRxiv - Cell Biology 2020Quote: ... Samples were loaded into 6% or 10% SDS-PAGE gels and stained with Coomassie Brilliant Blue (SIGMA) (5 µl from the eluate ...
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bioRxiv - Cell Biology 2020Quote: ... embryos/egg chambers were stained using 4′,6-diamidino-2-phenylindole (DAPI) (Sigma-Aldrich, St Louis, MO). Lipid droplets were stained using either Nile Red (Sigma-Aldrich ...
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bioRxiv - Immunology 2021Quote: ... after LPS-priming macrophages were treated for 6 h in OptiMEM with 1.5 µM nigericin (Sigma-Aldrich) or 1 µg/ml Clostridium difficile toxin B (Enzo Life Sciences ...
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bioRxiv - Developmental Biology 2021Quote: ... Slides were incubated in 4’,6-diamidino-2-phenylindole (DAPI) to label nuclei (1:10,000 dilution, Sigma). TUNEL assay was conducted with ApopTag Fluorescin Direct In Situ Apoptosis Detection Kit (Millipore ...
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bioRxiv - Genetics 2021Quote: ... cells underwent negative selection for 6 days with 1 ug/mL doxycycline (to induce promoter expression; Sigma), 100 ug/mL blasticidin S (to kill cells not expressing the blasticidin-resistant plasmid ...
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bioRxiv - Developmental Biology 2021Quote: ... the leaves were harvested and treated with 0.5 mg/ml DAPI (4ʹ,6-diamidino-2-phenylindole; Sigma). A Zeiss LSM 510 confocal scanning microscope was used to collect images.
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bioRxiv - Microbiology 2020Quote: ... cells were fixed with formaldehyde/PBS solution (6%) and stained with crystal violet (1%; Sigma Chemical Corp.) in 70% methanol ...