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Citations for Millipore Sigma :
2551 - 2600 of 4275 citations for 6 TERT BUTYLQUINOLINE 98.0+% GC T since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... The membranes were then blocked in 5% milk in Tris Buffered Saline with 1% Tween (TBS-T) overnight at 4° C before being probed overnight at 4° C with Rabbit anti Magi-2 (1:1000, Sigma M4221); Mouse anti-NR1 (1:1000 ...
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bioRxiv - Immunology 2024Quote: ... 100,000 to 200,000 T-allo10 cells were cultured in a 96-well plate for 6h in total of 200 uL of the base T cell medium (see Primary Cells section) with 3 ug/mL brefeldin A (Sigma Aldrich), 2 uM monensin (BD ...
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bioRxiv - Immunology 2023Quote: ... Cytokines were measured using the MILLIPLEX® Human CD8+ T Cell Magnetic Bead Panel Premixed 17 Plex-Immunology Multiplex Assay (Millipore) according to the manufacturer’s recommendations and analysis was done using the Bio-Plex Manager 6.1 software based on the standard curve.
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bioRxiv - Cell Biology 2022Quote: A431 (CRL-1555, ATCC), MDCK (CCL-34, ATCC) or HEK-293-T (CRL-3216) cells were cultured in DMEM (Sigma-Aldrich), supplemented with 10% Fetal Bovine Serum (Gibco ...
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bioRxiv - Molecular Biology 2023Quote: ... The FLAG-AGO1 proteins were detected with anti-FLAG-HRP (1:4000 [Sigma] in 5 % milk in PBS-T [0.05% tween20]) or anti-AGO1-N-coil (1:1000 in 5% milk in PBS-T ...
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bioRxiv - Biochemistry 2022Quote: ... All components were premixed and equilibrated for 15 min at 25 °C before being transferred in a dropwise manner into HEK 293 T cells grown in a 10-cm dish with 10 mL DMEM and 10 vol % fetal bovine serum (FBS) (Sigma SAFC). Media containing γ-retroviruses was harvested after 36-48 h incubation at 37 °C and 5% CO2 and was passed through 0.45 µm filters (Pall) ...
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bioRxiv - Cancer Biology 2023Quote: ... BSA in Tris buffered saline and Tween 20 (TBS-T) buffer for 1 h and incubated overnight with the following primary antibodies: Anti-LINE1 ORF1p (MABC1152) (EMD Millipore), Recombinant Anti-LINE1 ORF1p antibody [EPR21844-108] (Abcam) ...
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bioRxiv - Microbiology 2023Quote: ... T-REx-293 and HEK-293T cells were cultured in DMEM media (Dulbecco’s Modified Eagle’s Medium; Sigma-Aldrich, Gillingham, UK #D5796), whilst CHO-CAR and CHO-ACE2-TMPRSS2 cells were cultured in DMEM-F12 media (Dulbecco’s Modified Eagle’s Medium/Nutrient Mixture F-12 Ham ...
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bioRxiv - Microbiology 2023Quote: ... milk powder in TBS-T (Tris-buffered saline-Tween-20) and incubated overnight with primary antibody (monoclonal anti-FLAG, 1:1000; Sigma-Aldrich, #F1804-1MG ...
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bioRxiv - Immunology 2023Quote: ... the 3-day incubation period of in vitro T cell differentiation was conducted in culture medium containing 10mM of the antioxidant N-acetyl-cysteine (Sigma-Aldrich). For PI3K inhibitor treatment ...
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bioRxiv - Molecular Biology 2023Quote: ... PBMCs were stimulated with either LPS or T cell activation beads for 4 h followed by 12 h incubation with Brefeldin-A (Sigma-Aldrich). Following FACS anti-human antibodies were used for analysis ...
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bioRxiv - Bioengineering 2023Quote: ... Blocking was done for 30 minutes at room temperature in AbDil or TBS-T + 2 w/v% Bovine serum albumin (BSA, Sigma Aldrich) for 2D and in TBS-T + 5 w/v% BSA for 3D ...
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bioRxiv - Bioengineering 2023Quote: ... The membrane was submerged in secondary antibody solution (TBS-T + 5% BSA + 1:3000 Anti-Mouse IgG (Fab specific)-Peroxidase antibody (Sigma-Aldrich) and incubated for two hours at room temperature ...
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bioRxiv - Physiology 2023Quote: ... in PBS (BSA/PBS-T) for 1 hour prior to washing with PBS and incubation with primary anti-TH rabbit polyclonal antibody (EMD Millipore) at 1:300 BSA/PBS-T overnight at room temperature ...
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bioRxiv - Neuroscience 2023Quote: ... Non-specific sites were blocked by incubation with 5% BSA in Tris-buffered saline with 0.1% Tween® 20 detergent (TBS-T, 20 mM Tris-HCl and 150 mM NaCl, Sigma-Aldrich, Cat ...
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bioRxiv - Molecular Biology 2023Quote: ... 1×106/mL activated T cells were resuspended in TexMacs media and mixed with lentiviral supernatants and 13 μg/mL Polybrene (Sigma, USA). Dasatinib (50 nM ...
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bioRxiv - Molecular Biology 2023Quote: HDF cells transfected with the designated plasmid or microRNA were fixed with 4% paraformaldehyde (T&I, BPP-9004) and permeabilized with 0.3% Triton X-100 (Sigma-Aldrich, X100). Blocking was performed with 1% BSA (AMRESCO ...
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bioRxiv - Neuroscience 2023Quote: ... Blots were blocked in 5% dried milk in phosphate-buffered saline with 0.05% Tween-20 (PBS-T) (Sigma, St Louis, USA) and incubated with antibodies against S21-phosphorylated GSK3α (1:2000 ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were then cultured for 3 h in T cell stimulation media (1000ng/mL PMA [Sigma Aldrich, Catalog no. P1585-1MG], 2µM Ionomycin [Sigma Aldrich, Catalog no ...
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bioRxiv - Molecular Biology 2023Quote: ... Bound NS5A antibody was detected after two washes with PBS-T by incubation with horseradish peroxidase (HRP)-conjugated anti-mouse antibody (A4416, Sigma-Aldrich) diluted 1:300 in PBS-T ...
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bioRxiv - Cancer Biology 2023Quote: ... To pcw-KRAS-G12D-blast vector was packaged into a viral assembly by co-transfecting with PMD2.G (addgene, 12259) and PSPAX2 (addgene,12260) into HEK293 T cells using polyethyleneimine (PEI) transfection reagents (Sigma 408727). The virus-containing medium was used to infect HPNE E6/E7/st40 cells with 8ug/ml polybrene ...
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bioRxiv - Plant Biology 2024Quote: ... Probed membrane was washed with TBS-T thrice before incubating with alkaline phosphatase-conjugated anti-mouse secondary antibody (1:10000 dilution, Sigma, USA) or goat anti-rabbit IgG (H+L ...
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bioRxiv - Microbiology 2024Quote: ... the proteins on the membrane were stained with primary antibodies diluted in PBS-T (1x PBS with 0.2% (v/v) Tween 20 (Sigma-Aldrich, #P9416) and 0.1% (v/v ...
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bioRxiv - Cancer Biology 2024Quote: ... Slides were then washed twice 2 min in distilled water and 2 min in 1X Phosphate Buffered Saline with 0.1% Tween-20 (PBS-T) (PBS: Bichsel, 100 0 324, Tween-20: Sigma, P1379-500ML). Each tissue section was then covered with the primary antibody solution (Monoclonal antibody to human cytokeratin (pan ...
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bioRxiv - Cancer Biology 2024Quote: ... washed with TBS-T (TBS + 0.05% Tween20) (3 times x 2 minutes) and incubated with secondary antibodies and Hoescht 33342 (Sigma-Aldrich, #14533) diluted in 1x Antibody diluent/Block solution for 2 hours at room temperature ...
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bioRxiv - Immunology 2024Quote: ... Plates were washed three times with 200 μL of PBS-T and developed using 100 μL/well of o-phenylenediamine dihydrochloride (OPD, Sigma P8287) for 10 min ...
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bioRxiv - Immunology 2024Quote: ... Plates were subsequently washed three times with 300 µl/well of PBS containing 0.05% Tween 20 (PBS-T) (Sigma-Aldrich, USA). Non-specific binding sites were blocked using 300 µl/well of 3% (w/v ...
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bioRxiv - Cancer Biology 2024Quote: Cells were seeded in a 96-well plate at a density of 15,000 cells per well in 50 µL of medium containing the HDAC substrate N-(4-Methyl-7-aminocoumarinyl)-Nα-(t-butoxycarbonyl)-Nω-acetyllysineamide (21 μM, MAL, Sigma-Aldrich) and incubated for 8 h at 37 °C ...
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bioRxiv - Cancer Biology 2024Quote: ... Cells (named T-ALL#2) were frozen and conserved in 90% Fetal Calf Serum (FCS) and 10% DMSO solution (D5879, Sigma Aldrich), or injected into CD42.1.2 C57BL/6 recipient mice ...
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bioRxiv - Molecular Biology 2024Quote: ... and the membrane blocked with 5% skim milk powder dissolved in PBS-T (1x PBS with 0.1% Tween-20 (Sigma-Aldrich #P1379)) for ∼1 h at room temperature ...
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bioRxiv - Cell Biology 2019Quote: ... while chromatin was visualized with DAPI (4’, 6-diamidino-2-phenylindole dihydrochloride, 1μg/ml Sigma-Aldrich). For imaging of live embryos for real-time promoter analysis using G-TRACE or JNK signaling ...
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bioRxiv - Cell Biology 2020Quote: ... HCT116 cells were transduced by using the above lentiviral particles with polybrene (6 μg/mL, Sigma). After 24 hr ...
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bioRxiv - Cell Biology 2020Quote: 6×106 INS-1E cells were seeded out in tissue culture flasks (TPP®, Sigma-Aldrich) a day prior to transient transfection ...
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bioRxiv - Molecular Biology 2021Quote: ... and input TRF1 mutants were revealed with anti-His (anti 6-His Rabbit Pab Sigma Aldrich). For the detection of biotin-labelled PARylated proteins the same assay was conducted in presence of biotin-NAD+ (Sigma Aldrich ...
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bioRxiv - Developmental Biology 2021Quote: ... Primary antibodies for cilia (mouse anti-acetylated tubulin, 1:1000 dilution, Sigma, cat# 6-11B-1) were diluted in block buffer and incubated on slides for 2hr at RT or overnight at 4°C ...
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bioRxiv - Developmental Biology 2021Quote: ... pooled (n=6) and homogenized in Tris-SDS lysis buffer with protease inhibitor (Sigma-Aldrich, USA). 10% homogenates were assayed for total protein content by Bradford method (1976) ...
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bioRxiv - Microbiology 2022Quote: ... Cells were treated for 6 h with the appropriate concentration of either poly(I:C) (Sigma, P1530) or High Molecular Weight (HMW ...
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bioRxiv - Developmental Biology 2022Quote: ... and mouse anti-acetylated α-tubulin (clone 6-11B-1, Millipore-446 Sigma, #MABT868, 1:500) were diluted in 5% normal goat serum (NGS ...
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bioRxiv - Developmental Biology 2022Quote: ... and mouse anti-acetylated α-tubulin (clone 6-11B-1, Millipore-446 Sigma, #MABT868, 1:500) were diluted in 5% normal goat serum (NGS ...
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bioRxiv - Neuroscience 2020Quote: ... and the AMPA receptor antagonist 6-cyano-7-nitroquinoxaline-2,3-dione (CNQX, 10 μM, Sigma Aldrich), for 2 min at room temperature ...
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bioRxiv - Cell Biology 2022Quote: ... cells were kept for 6 days in medium containing 1.25% DMSO (cell-culture grade; Sigma-Aldrich), and the differentiation status was validated using flow cytometry (CD11b ...
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bioRxiv - Immunology 2021Quote: ... 3 μg pRSV-Rev and 6 μg of pRRL lentiCRISPR vectors using Poly(ethyleneimine) (PEI; Sigma). Media was replaced 24 hours post-transfection and harvested 24 hours later for filtration with a 0.45 μm filter (SteriFlip ...
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bioRxiv - Developmental Biology 2019Quote: ... This was followed by incubation with 4’,6-diamidino-2-phenylindole (DAPI; Sigma-Aldrich; 1:10000) for 10 mins at room temperature ...
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bioRxiv - Biochemistry 2019Quote: ... Nuclei were digested for 6 minutes with 0.25 U MNase/mL (Sigma-Aldrich cat #N3755-500UN) and supplemented with 1.5 mM CaCl2 ...
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bioRxiv - Immunology 2019Quote: ... the plates were incubated with 2,2’-azino-bis (3-ethylbenzthiazoline-6-sulfonic acid) (A3219; Sigma-Aldrich) at room temperature for 30 min ...
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bioRxiv - Cell Biology 2019Quote: ... After washing off secondary antibodies 4′,6 diamidino-2-phenylindole (DAPI) (10μg/ml in PBS, Sigma) was used to visualize the nuclei ...
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bioRxiv - Cell Biology 2019Quote: ... The nuclei were counter-stained with 1 μg/ml DAPI (4′,6-diamidino-2-phenylindole, Sigma) for 5 minutes ...
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Genomic organization of the autonomous regulatory domain of eyeless locus in Drosophila melanogasterbioRxiv - Genetics 2021Quote: ... and finally stained for 4-6 h in lacZ staining solution with 0.2% X-gal (Sigma). The stained embryos were imaged in Leica stereomicroscope ...
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bioRxiv - Immunology 2021Quote: ... Plates were washed 6 times with washing buffer (1× PBS with 0.05% Tween-20 (Sigma-Aldrich)) and incubated with 170 μl per well blocking buffer (1× PBS with 2% BSA and 0.05% Tween-20 (Sigma) ...
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bioRxiv - Systems Biology 2020Quote: ... aliquots of calcein NVOC (6 M in DMSO) were diluted 1:100 in PEG200 (Sigma-Aldrich), then in PBS to a final concentration of 1:5,000 for photoactivation of organoids and tissue sections ...