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Citations for Millipore Sigma :
2701 - 2750 of 10000+ citations for Recombinant Human MSLN Protein Fc tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... Plates were washed 4 times with PBS/Tween and then incubated for 1 h at RT with 100 μL/well of an anti-mouse Fc conjugated to alkaline phosphatase (Sigma-Aldrich), diluted 1:1000 in blocking buffer ...
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bioRxiv - Immunology 2022Quote: Each SMG pair (approx. 160mg) was mechanically minced in 2mL of RPMI-1640 containing 5% fetal calf serum (FCS; Sigma Aldrich), 25µl Collagenase-II (23mg/mL ...
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bioRxiv - Immunology 2019Quote: ... Cells were plated at 0.5-1 × 105 cells in a 96 well plate in L-15 media containing 5% FCS and exposed to DMSO (vehicle control) or 50 ug/ml 17-β estradiol or Bis-phenol A (Sigma) for 1 h at 37°C ...
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bioRxiv - Cell Biology 2019Quote: ... Afterwards the medium was switched and cells were cultivated for 4 days in DMEM containing 10% FCS and 2 μM insulin (Sigma Aldrich) to induce adipogenic differentiation ...
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bioRxiv - Pathology 2020Quote: SFs were seeded in 6-well plates at 1×106 cells per well and synchronised overnight in 1% FCS DMEM before being treated for 24 hours with the indicated stimuli: LPS (1 µg/ml, Sigma-Aldrich), IL-1β (10ng/ml ...
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bioRxiv - Cell Biology 2021Quote: ... [42] After expansion in complete A-RPMI, pUC were cryopreserved in heat inactivated fetal calf serum (FCS, Biochrom) supplemented with 10 % Dimethyl sulfoxide (DMSO, Sigma Aldrich) until use ...
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bioRxiv - Immunology 2020Quote: ... cells were expanded in selection medium (10 % dialysed FCS, 1x GSEM supplement [Sigma-Aldrich], 20–50 μM MSX, 1 % Pen/Strep) for at least 1 week ...
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bioRxiv - Immunology 2021Quote: ... cells were expanded in selection medium (10 % dialysed FCS, 1x GSEM supplement [Sigma-Aldrich], 20–50 µM MSX, 1 % Pen/Strep) for at least 1 week ...
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bioRxiv - Immunology 2021Quote: ... 721.221 was purchased from the International Histocompatibility Working Group and grown in RPMI-1640 supplemented with 15% heat-inactivated fetal calf serum (FCS) (Sigma-Aldrich), sodium pyruvate (Thermo Fisher) ...
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bioRxiv - Immunology 2020Quote: Urine cells were thawed into 9 ml of 10% FCS-RPMI medium supplemented with 10 IU/ml Benzonase (Novagen 70664-3), counted and viability determined by trypan blue exclusion ...
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bioRxiv - Immunology 2019Quote: Small intestinal biopsies were collected at the time of endoscopy and transported in R10 (RPMI-1640 [Lonza] + 10% FCS [Sigma-Aldrich] + 1% penicillin/streptomycin [Sigma-Aldrich]) ...
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bioRxiv - Cell Biology 2020Quote: ... OMP-18R5 (OncoMed Pharmaceuticals, Redwood City, CA) at 10μg/mL with antihuman IgG (Fc) secondary conjugated to FITC (Millipore Sigma AP113F).
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bioRxiv - Immunology 2020Quote: ... Freshly isolated PBMCs from four donor M1/M2 MCM were pooled and resuspended in RPMI-1640 containing 10% fetal calf serum (FCS; R10) and 5 ug/ml phytohemagglutinin-P (Sigma-Aldrich). The culture was incubated overnight at 37°C in a 5% CO2 atmosphere ...
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bioRxiv - Immunology 2020Quote: ... and 2.5 ng/mL TNF-α for 3-5 days and subsequent differentiation in RPMI (Sigma, St Louis, Mo; +10% FCS) either with 100 ng/mL GM-CSF ...
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bioRxiv - Immunology 2022Quote: ... fibroblasts and MCF 10A cells were incubated with the FITC bound anti-mouse IgG secondary immunoglobulin (Fc specific) (Cat # F4143, Sigma-Aldrich) secondary antibody for 2 h at room temperature away from light ...
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bioRxiv - Cell Biology 2022Quote: ... A HeLa cell line stably expressing connexin43 [47] grown in DMEM/10%FCS/P/S supplemented with 1 μg/ml puromycin (Sigma #P9620) were solubilized in 1% Chaps/TBS/5% glycerol at pH 7.4 supplemented with protease blockers ...
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bioRxiv - Synthetic Biology 2022Quote: ... Single-cell suspensions were derived by mechanical disruption of bone marrow in PBS supplemented with 2% Fetal Calf Serum (FCS, Sigma, C8056). For FACS analysis ...
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bioRxiv - Microbiology 2022Quote: ... and suspended at 2 x 104 cells/mL in Titan Cell Medium (TCM) composed by 5% Sabouraud, 5% inactivated Fetal Calf Serum (FCS, Biological Industries) and 15 µM sodium azide (Sigma Aldrich), diluted in 50 mM MOPS adjusted to pH 7.3 ...
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bioRxiv - Microbiology 2022Quote: MPXV strains were diluted ½ in two different kinds of media that is Viral Transport Media (VTM; 330C, Copan) and FCS (F2442, Sigma-Aldrich). Two hundreds microliters of each sample were submitted in triplicate to various temperatures for different periods of time ...
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bioRxiv - Microbiology 2024Quote: ... BEAS-2B cells grown in 6-wells plates were first transduced with lentiviruses encoding Cas9 diluted in infection medium (DMEM, 2% FCS) containing 8 μg/ml Polybrene (Sigma Aldrich). At 16 h post transduction ...
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bioRxiv - Microbiology 2023Quote: ... Isolates were standardised to 1×106 cells/mL in Roswell Park Memorial Institute (RPMI) media supplemented with 5% (v/v) foetal calf serum (FCS [Sigma-Aldrich]) to simulate organic load ...
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bioRxiv - Molecular Biology 2024Quote: ... Negative selection was therefore carried out by culturing the parasites in the presence of 5-fluorocytosine (5-FC, Sigma-Aldrich, UK) at 48 mM and 2.5 nM WR99210 for a week ...
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bioRxiv - Immunology 2024Quote: ... sections were washed with PBS to remove OCT compound on the slides, and blocked with solutions containing imaging buffer (2% FCS, 0.1% Triton X-100 (Sigma, Cat. X100), 0.01% sodium azide) ...
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bioRxiv - Immunology 2023Quote: ... guts were transferred to a new 50 mL tubes containing 1x digestion mix (RPMI GlutaMAX™ containing 2% FCS, 1mg/mL of Colagenase VIII [Sigma] and 40 u/mL of DNAse I [Sigma]) and incubated for 20 min at 37 °C sin an orbital shaker at 200 rpm ...
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bioRxiv - Immunology 2023Quote: ... before counting in complete RMPI (cRPMI = naRPMI supplemented with 10% heat-inactivated FCS [#S-FBS-EU-015, Serana, Pessin, Germany], 25 nM β-mercaptoethanol [#M6250, Sigma] ...
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bioRxiv - Genetics 2023Quote: ... the tissue slurry was seeded directly on Matrigel coated dishes (45 μg/cm2) in DMEM/F12 containing 20%FCS (Sigma-Aldrich) and 10% horse serum (Sigma-Aldrich ...
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bioRxiv - Biochemistry 2022Quote: ... under agitation at room temperature for 20 min and re-probed with HRP-conjugated poly-clonal goat anti-mouse IgG Fc (1:5000 dilution, Sigma–Aldrich) for checking the integrity of the mouse IgG2b Fc domain of the fusion protein ...
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bioRxiv - Microbiology 2023Quote: ... ∼8 x 106 parasites were pelleted by centrifugation at 800 rcf for 7 min and resuspended in FCS-free HMI-9 media with 1 µg/mL Hoechst 33342 (Sigma-Aldrich). Cells were pelleted again ...
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bioRxiv - Immunology 2023Quote: ... Cells were re-suspended at 1×107 cells/ml in RPMI 1640 buffer supplemented with 10% foetal calf serum (FCS, Sigma-Aldrich) for further analysis ...
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bioRxiv - Cancer Biology 2023Quote: ... AML cells were washed and cultured in Gartner’s medium (Alpha-MEM, 12.5% horse serum, 12.5% FCS, 1μM hydrocortisone (Sigma-Aldrich, St. Louis, MO, USA), 1% pen-strep (Sigma-Aldrich ...
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bioRxiv - Immunology 2023Quote: ... The tissue was incubated in RPMI/5%FCS/10mMHEPES for 5min before being digested in 10ml RPMI/5% FCS/10mM HEPES supplemented with 0.4mg/ml type VIII collagenase (Sigma Aldrich, UK) and 40μg/ml DNase I (Roche ...
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bioRxiv - Immunology 2023Quote: ... samples were serially diluted in MEM containing 2% FCS and 100 Units Penicillin / 0.1 mg Streptomycin (P/S) (Millipore Sigma, Germany). Vero E6 cells were incubated with 100 µl of ten-fold sample dilutions added in quadruplicates for 1 h at 37°C before 100 µl MEM containing 2% FCS and P/S were added per well and plates were incubated for 5 days at 37°C and 5% CO2 ...
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bioRxiv - Synthetic Biology 2023Quote: ... Fc-PRL-13-c6 was grown in 200mL of BMGY media supplemented 1tab/50mL of cOmplete Protease Inhibitor Cocktail (Sigma; 11836145001) and 10uM Pepstatin A (Sigma ...
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bioRxiv - Immunology 2023Quote: ... were co-cultured in round-bottomed 96-well plates in DC medium (RPMI-1640, 10% FCS, 100 U/mL Penicillin, 100 μg/mL Streptomycin, 2 mM L-glutamine [Sigma-Aldrich]) the presence of indicated concentrations of OVA323-339 peptide (Sigma ...
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bioRxiv - Immunology 2023Quote: ... These PBMCs were then re-suspended in large volumes of RPMI 1640 medium containing 5% v/v fetal calf serum (FCS) (F-0926, Sigma, USA) and centrifuged against at 500 x g and after a further wash their PMBCs were re-suspended in ice-cold 10% dimethyl sulfoxide (D-2650 ...
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bioRxiv - Immunology 2023Quote: ... THP1 dual reporter cells were maintained in RPMI (Nacalai Tesque Inc.,Kyoto, Japan) supplemented with 10% FCS (Sigma-Aldrich, Missouri, USA), 1% penicillin/streptomycin (Nacalai Tesque Inc.,Kyoto ...
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bioRxiv - Cancer Biology 2023Quote: ... The gradients were then displaced into a UA-6 continuous UV detector (Teledyne ISCO) using a syringe pump (Brandel) containing Fluorinert FC-40 (Sigma #F9755) at a speed of 0.75 ml/min ...
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bioRxiv - Cancer Biology 2023Quote: ... Newly diagnosed leukemic cells was partly used directly after sampling for in vitro and in vivo experiments and partly frozen in Foetal Calf Serum (FCS) containing 10% of Dimethylsufoxide (DMSO) (D8418, Sigma-Aldrich). Human T-ALL samples are described in Supplementary Table 1.
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bioRxiv - Cancer Biology 2024Quote: ... Cells (named T-ALL#2) were frozen and conserved in 90% Fetal Calf Serum (FCS) and 10% DMSO solution (D5879, Sigma Aldrich), or injected into CD42.1.2 C57BL/6 recipient mice ...
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bioRxiv - Microbiology 2024Quote: ... The cells were maintained with DMEM+10%FCS for 3 hours and fixed with acetone/methanol (1:1 ratio) and stained with Giemsa stain (Sigma-Aldrich). Stained cell images were taken by EVOS XL imaging system (Life Technologies).
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bioRxiv - Synthetic Biology 2024Quote: ... we first prepared a 2% (w/w) mixture of surfactant (008 FluoroSurfactant, RAN Biotechnologies) and oil (FC-40 FluorinertTM, Sigma-Aldrich). Using Eppendorf DNA Lobind® tubes ...
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bioRxiv - Cancer Biology 2024Quote: ... Cells were washed with warm PBS + 1%FCS and incubated with 1:50 DAPI (70µg/ml, Sigma-Aldrich, cat. No. D9542) and 1:50 Annexin V-APC (Biolegend ...
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bioRxiv - Cell Biology 2021Quote: ... at a concentration of 1:500 was used to detect myc tagged ATR and incubated at room temperature for 1 h in blocking buffer (1 % BSA [Sigma], 0.2 % Tween-20 in 1x PBS) in a wet chamber ...
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bioRxiv - Microbiology 2020Quote: ... T cells were then infected with HIVBaL and cultured for up to 7 days in RPMI medium additionally supplemented with 10 U/ml recombinant interleukin-2 (Sigma). The dividing cell lines HEK293T (ATCC ...
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bioRxiv - Biochemistry 2021Quote: ... standard conditions (ii) with or without recombinant enzyme Agalsidase alpha (5µl/ml) (Takeda) and (iii) with or without wortmannin (75nM) (Sigma-Aldrich). Of note ...
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bioRxiv - Neuroscience 2021Quote: ... Sample was then diluted 8-fold with 50 mM HEPES pH 8.0 and digested by the addition of 5 μg TrypZean recombinant trypsin (Sigma-Aldrich) and incubation for 8 hours at 30 °C ...
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bioRxiv - Plant Biology 2021Quote: ... residual trypsin activity was measured by monitoring the change in absorbance at 247 nm in presence of increasing concentration of recombinant purified Kunitz Trypsin inhibitor (KTI) when incubated with p-toluene-sulfonyl-L-Arg methyl ester (Sigma). To study the inhibitory action of P235 on KTI action ...
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bioRxiv - Plant Biology 2021Quote: ... Substrate specificity of recombinant AtPME2 activity was determined at pH 7.5 and 28°C using commercial citrus pectin (Sigma-Aldrich, DM >85% ...
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bioRxiv - Systems Biology 2020Quote: ... At time 0 medium was removed from cells and recombinant mouse tumor necrosis factor alpha (TNF-α, Sigma-Aldrich, #T7539) was added at concentrations 0-100 ng/mL as 5-minute pulse ...
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bioRxiv - Plant Biology 2021Quote: ... substrate specificity assays for recombinant PcIBMS1 and PcIPMS1 were determined with a spectrophotometric end-point assay with DTNB (Sigma-Aldrich). An aliquot of 1-5 μL of the protein preparation was added to an enzyme assay mixture (100 mM Tris ...