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Citations for Millipore Sigma :
2651 - 2700 of 10000+ citations for Recombinant Human MSLN Protein Fc tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2021Quote: ... parental SK-N-SH cells were trypsinized and resuspended in grafting media in presence of 10μg/ml recombinant NXPH1 (rNXPH1) or 100μg/ml BSA (Ctrl; SIGMA #A7906). To test the growth of NB cells depleted from their α-NRXN1+ subpopulation ...
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bioRxiv - Cell Biology 2019Quote: A GFP-expressing MDA-MB-231 cell line lacking functional Nav1.5 expression was produced previously by transduction of recombinant lentivirus for shRNA targeting Nav1.5 (MISSION pLKO.1-puro shRNA transduction particles; Sigma) (Nelson ...
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bioRxiv - Pathology 2020Quote: ... each recombinant tau isoform was thawed at room temperature and filtered with a 100 kDa spin column filter (Millipore) to remove unwanted tau aggregates ...
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bioRxiv - Microbiology 2021Quote: ... neoformans cells were added to washed macrophage monolayers at a 1:1 ratio with 10 units (1 unit = 0.1 ng/mL) of recombinant murine IFNγ (Sigma) and 0.3 μg/ml of lipopolysaccharide (Sigma ...
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bioRxiv - Microbiology 2020Quote: ... Recombinant E1E2 was extracted from cell membranes using 1% NP-9 and purified via sequential Fractogel EMD TMAE (Millipore), Fractogel EMD SO3− (Millipore) ...
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bioRxiv - Cell Biology 2021Quote: BMDC and BMLC maturation was induced by overnight treatment with 100 ng/ml recombinant murine TNFα (Sigma-Aldrich, USA). In some experiments ...
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bioRxiv - Cell Biology 2021Quote: ... Rose-John), 20 U/ml recombinant murine GM-CSF (PharmedArtis, Germany) and 10−6 M dexamethasone (Sigma-Aldrich, USA). Progenitor cells were amplified 5 to 11 days before differentiation into DC was induced by supplementing 200 U/ml recombinant murine GM-CSF ...
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bioRxiv - Biophysics 2022Quote: ... a heparosan trisaccharide amine derivative was extended with recombinant MBP-PmHS1 synthase using UDP-GlcNAc and UDP-GlcA (Sigma) [48] ...
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bioRxiv - Biophysics 2020Quote: ... beads were removed and recombinant dynein was concentrated in a 100 KDa cut-off filter (Amicon Ultracel, Merck-Millipore) to a final volume of 500 μL ...
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bioRxiv - Immunology 2022Quote: ... 20 µM DTT and phosSTOP in the presence of recombinant cyclin A2/cyclin-dependent kinase (CDK)1 (Sigma-Aldrich) and 10 mM ATP incubated for 2 h at 30 °C ...
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bioRxiv - Microbiology 2022Quote: ... Recombinant SIRV2 gp21 and SiL_0190 with C-terminal His-tag were expressed using Escherichia coli RosettaTM (DE3) cells (Novagen). Protein expression was induced during logarithmic growth through the addition of 0.5mM isopropyl-thiogalactopyranoside (IPTG ...
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bioRxiv - Immunology 2023Quote: ... SRBCs in Alsever’s (TCS Bioscience) were conjugated to recombinant HEL3X (R. Brink) with 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride (Sigma) as previously described104 ...
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bioRxiv - Neuroscience 2023Quote: Primary rat cortical cultures were treated with recombinant Tat 1-86 (Diatheva, Italy) and with cocaine-HCl (Sigma Chemicals) as described in each Figure Legend ...
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bioRxiv - Biochemistry 2023Quote: ... Fractions containing recombinant PKAc verified via SDS-PAGE were pooled and concentrated to 250μL using an Amicon concentrator (10K MWCO; Millipore). The concentrated sample was then applied to a preparative-grade Superdex200 column (GE Healthcare ...
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bioRxiv - Developmental Biology 2021Quote: ... Even protein loading and successful protein transfer was confirmed using Ponceau S stain (Sigma Aldrich) prior to probing with primary antibodies (Table S2) ...
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bioRxiv - Cell Biology 2022Quote: ... for total protein loading quantification before proteins were transferred to PVDF membrane (Millipore, Cat. # IPVH00010). The blots were incubated with primary antibodies against SUMO2/3 (Enzo Life Sciences ...
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bioRxiv - Immunology 2021Quote: ... with protein concentrations normalized to total protein levels in lysate (BCA assay; Sigma-Aldrich®).
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bioRxiv - Microbiology 2019Quote: ... and the protein level was measured with the Enhanced BCA Protein Assay kit (Sigma-Aldrich). The extracts which contained 25 μg of total proteins were subjected to 10% SDS polyacrylamide gel ...
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bioRxiv - Cell Biology 2021Quote: ... and the protein concentration was estimated using the Bio-Rad Protein Assay Dye Reagent (Sigma). 10 µl of liver lysate was transferred to 96-well plate and ALT was measured using a microplate-based ALT activity assay kit (Pointe Scientific ...
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bioRxiv - Cell Biology 2020Quote: Proteins of cellular membrane were extracted using the ProteoExtract® Transmembrane Protein Extraction Kit (NOVAGEN). Total 500 μg of membrane proteins were used for immunoprecipitation with an anti AT1-R or LOX-1 antibody ...
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bioRxiv - Cell Biology 2019Quote: ... Protein concentration was determined using a BCA protein concentration assay kit (Sigma, Shanghai, China, FP0010). Equal amounts of protein (20 μg ...
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bioRxiv - Cancer Biology 2020Quote: ... the concentration of EV protein was quantified by using a BCA Protein Assay Kit (Novagen). For BMDC stimulation by EVs ...
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bioRxiv - Cancer Biology 2021Quote: ... the protein-protein interaction was identified using a commercially available PLA kit (Duolink; Sigma-Aldrich) as described before 34 ...
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bioRxiv - Molecular Biology 2019Quote: ... Protein complexes were pulled down by 1.5 h incubation with Protein A agarose beads (Millipore) or GFP-Trap®_A beads (Chromotek) ...
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bioRxiv - Cell Biology 2020Quote: Proteins from 3T3-L1 fibroblast lysates extracted with Cytobuster protein extraction reagent (Novagen®, Merck). After cell disruption for 10 min at room temperature ...
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bioRxiv - Plant Biology 2024Quote: ... Proteins were separated by SDS-PAGE and biotinylated proteins detected using Streptavidin-HRP (Sigma # RABHRP3) and Supersignal detection kit (Thermofisher Scientific) ...
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bioRxiv - Molecular Biology 2024Quote: ... The fusion protein was treated with SUMO protease (Sigma SAE0067, 5 U/ mg target protein) to liberate Mpro with authentic N-(Ser1 ...
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“Identification of microRNAs regulated by E2F transcription factors in human pluripotent stem cells”bioRxiv - Developmental Biology 2024Quote: ... Total proteins extraction from hPSCs was performed in ice-cold RIPA protein extraction buffer (Sigma) supplemented with protease inhibitors (Protease inhibitor cocktail set I ...
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bioRxiv - Plant Biology 2023Quote: ... Protein levels were then quantified using a Coomassie protein assay kit (B8522, Sigma-Aldrich, USA) followed by 12% SDS-PAGE separation (Bio-Rad ...
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bioRxiv - Cell Biology 2023Quote: ... Protein concentrations were then normalized to 6 μg protein and the KGDH assay kit (Sigma) protocol was followed.
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bioRxiv - Cancer Biology 2023Quote: ... The protein-protein interaction was investigated using a commercially available PLA kit (Duolink; Sigma-Aldrich) following the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2023Quote: ... Protein concentrations were determined by Bicinchoninic Acid Kit for Protein Determination (BCA1-1KT, Sigma Aldrich) or the Bradford Protein Assay (BioRad) ...
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bioRxiv - Cancer Biology 2023Quote: ... and protein concentration in the supernatant was measured using the BCA Protein Assay kit (Sigma).
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bioRxiv - Molecular Biology 2024Quote: ... Lysates were pre-cleared by Protein G Plus/Protein A Agarose Suspension (Merck Millipore #IP05). The lysates were then separately incubated with truncated form antibodies (i ...
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bioRxiv - Cancer Biology 2024Quote: ... The protein concentration was then determined using a Pierce BCA protein assay (Sigma-Aldrich; 71285) and intact cell respiration was normalized to milligrams of total intact cell protein.
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bioRxiv - Cancer Biology 2024Quote: ... The protein concentration was then determined using a Pierce BCA protein assay (Sigma-Aldrich; 71285).
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bioRxiv - Developmental Biology 2021Quote: ... The proximal forelimbs of embryos were dissected (see Figure 3) and placed into PBS with 10% FCS and 8ul collagenase at 50mg/ml (Sigma C9697) at 37° for approximately 5min ...
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bioRxiv - Molecular Biology 2022Quote: ... followed by incubation with 3-isobutylmethyl-xanthine (1 mM)-containing medium or including the secondary antibody anti-mouse IgG (Fc specific, M2650, Sigma-Aldrich) for 30 min ...
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bioRxiv - Microbiology 2019Quote: ... (ATCC) cells were maintained in DF-1 cell culture media (DMEM supplemented with 10% FCS, 5% tryptose phosphate broth (Sigma-Aldrich), 1% NEAAs and 1% penicillin-streptomycin and maintained at 39 °C in a 5% CO2 atmosphere.
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bioRxiv - Cell Biology 2019Quote: ... purified DN3a cells were cultured on OP9-DL1 stromal cells or Fc-DL1 functionalised surfaces in the presence of AMD3100 (2 μg/ml, Sigma-Aldrich) or DBZ (0.05 µM ...
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bioRxiv - Genetics 2021Quote: ... EDL muscles were digested in Krebs solution (without FCS) containing 3 mg/ml collagenase type IV A (Sigma Aldrich, MO, USA), gently bubbled with carbogen (95% O2 ...
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bioRxiv - Cancer Biology 2020Quote: ... supplemented with 10 % Foetal Calf Serum (FCS, LabTech International Ltd, Heathfield, East Sussex, UK) and 4 mM L-glutamine (Sigma-Aldrich).
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bioRxiv - Biochemistry 2020Quote: ... and Anti-Mouse IgG (Fc specific)-FITC antibody produced in goat (Cat # F4143, Sigma Aldrich, USA; 30 min at 4°C).
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bioRxiv - Neuroscience 2021Quote: 4% PFA-fixed brains were washed twice in 20 mL PBS for a total of 24 hours and imaged in Fluorinert FC-40 (Sigma Aldrich) for null background signal ...
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bioRxiv - Bioengineering 2021Quote: Each intact piglet brain hemisphere was placed in a 30 mm diameter glass tube containing perfluorocarbon oil (Fluorinert FC-40, Sigma-Aldrich) as a magnetic susceptibility matching fluid ...
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bioRxiv - Microbiology 2021Quote: ... gondii parasites were cultured in complete medium (DMEM, +5 % FCS) or in custom culture medium −Pan (DMEM −Pan, +5 % dialyzed FBS) supplemented with Val (Sigma, V6504), pantoate (Sigma ...
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bioRxiv - Microbiology 2020Quote: ... Thymic fragments were washed in RPMI 2% FCS and digested 15 min at 37 °C in 500 μg/mL Liberase TL (Sigma-Aldrich) and 111 μg/mL DNase I from bovine pancreas (Sigma-Aldrich) ...
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bioRxiv - Biophysics 2020Quote: ... in 20 ml Dulbecco’s Modified Eagle Medium (DMEM, Capricorn, Ebsdorfergrund, Germany) containing 10 % fetal calf serum (FCS, Sigma, St. Louis, USA) and 1 % penicillin-streptomycin (Gibco ...
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bioRxiv - Immunology 2022Quote: ... Any larger tissue fragments were dissociated mechanically followed by washing of the cell strainer with MACS buffer (PBS, 2% FCS, 2mM EDTA (Sigma-Aldrich)) ...
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bioRxiv - Immunology 2021Quote: ... Wells were washed 3 times with washing buffer then 100 μL of diluted goat anti-mouse IgG (Fc specific)-Peroxidase antibody (#A0168; Sigma-aldrich) were added ...