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Citations for Millipore Sigma :
2651 - 2700 of 10000+ citations for Rat Macrophage expressed gene 1 protein MPEG1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... Protein was trypsin (Sigma Aldrich) digested at 37°C for 18 hours ...
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bioRxiv - Cell Biology 2023Quote: ... with protein inhibitor cocktail (Sigma) and subsequently separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE ...
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bioRxiv - Cancer Biology 2022Quote: For RNA-binding protein immunoprecipitation (RIP) ~3×107 A549 cells for each sample were processed using Magna RIP™ RNA-Binding Protein Immunoprecipitation Kit (Millipore Sigma) following the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2020Quote: Detection of an interaction between BRCA1 and the cavin or CAV1 proteins was assessed using the Duolink™ II Detection Kit (Sigma Aldrich) according to the manufacturer’s specifications ...
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bioRxiv - Cancer Biology 2021Quote: RIP assays were conducted to explore the relationship between lncRNA NEAT1 and miR-1299 via the Magna RIP RNA-Binding Protein Immunoprecipitation Kit (Millipore, MA, USA). Initially ...
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bioRxiv - Bioengineering 2022Quote: For purification the Amicon® Pro-Affinity Concentration Kit Protein A with 100 kDa Amicon® Ultra-0.5 Device (Merck Millipore, UK) as used as described by the manufacturer ...
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bioRxiv - Genetics 2022Quote: ... The column was calibrated using a gel filtration markers kit for protein molecular weights 29,000-700,000 Da (Sigma-Aldrich, product number MWGF1000).
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bioRxiv - Biochemistry 2024Quote: The concentration of both activated proteins—PA-BFP and SumireF—was determined by the bicinchoninic acid assay using the QuantiPro™ Kit (Sigma–Aldrich), following the supplier’s instructions ...
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bioRxiv - Plant Biology 2024Quote: ... the total proteins in the samples were measured through the BiCinchoninic acid Assay using a kit (Sigma-Aldrich, Saint Louis, MO, USA). The assay was conducted according to the method provided by the manufacturer ...
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bioRxiv - Bioengineering 2023Quote: ... ProteoExtract® native membrane protein extraction kit and 10 kDa Amicon® Ultra-15 centrifugal filters were purchased from Millipore Sigma (USA). Assays for measuring cell toxicity were purchased from Promega (Madison ...
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bioRxiv - Cancer Biology 2023Quote: ... using either a control normal rabbit IgG or rabbit monoclonal anti-MSI2 antibody and the Magna RIP RNA-binding Protein Immunoprecipitation kit (cat#17-700, Millipore, Burlington, MA). Manufacturer’s instructions were followed with the exception that RNeasy MinElute Cleanup kit (cat#74202 ...
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bioRxiv - Cell Biology 2023Quote: ... using either a control normal rabbit IgG or rabbit monoclonal anti-MSI2 antibody and the Magna RIP RNA-binding Protein Immunoprecipitation kit (cat#17-700, Millipore, Burlington, MA). Manufacturer’s instructions were followed with the exception that RNeasy MinElute Cleanup kit (cat#74202 ...
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bioRxiv - Cell Biology 2020Quote: ... Lamin A proteins were detected with mouse anti-myc antibodies (clone 4A6, Millipore cat #05-724; 1:10,000 dilution) decorated with goat anti-mouse secondary IRDye 680RD antibodies (LI-COR ...
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bioRxiv - Microbiology 2021Quote: ... Sized proteins were brought to concentrations typically >50 mg mL−1 using appropriate molecular weight cut-off concentrators (Millipore), flash frozen on liquid nitrogen ...
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bioRxiv - Molecular Biology 2020Quote: ... The appropriate horseradish peroxidase-coupled secondary antibodies were used to reveal the proteins (antimouse at 1:10,000 (Sigma, A2554) and antirabbit at 1 ...
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bioRxiv - Cell Biology 2022Quote: ... were added for 1 hour at room temperature and protein signal was then visualized using Immobilon Forte (Millipore, WBLUF0500) on ImageQuant LAS 4000 or Amersham ImageQuant 800 ...
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Glyoxal Does Not Preserve Cellular Proteins as Accurately as PFA: A Microscopical Survey of EpitopesbioRxiv - Cell Biology 2019Quote: ... Then slides were incubated with antibodies specific to acrosin binding protein (ACRPB) (1:100 in PBS) (Sigma; HPA039081, USA), IZUMO1 (1:100 in PBS ...
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bioRxiv - Neuroscience 2019Quote: ... proteins were transferred electrophoretically to polyvinylidene difluoride membranes and probed with the following antibodies:anti-EphB6 (1:500, SAB4503476, Sigma), anti-EphB1 (1:500 ...
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bioRxiv - Molecular Biology 2019Quote: ... Bound proteins were cleaved off the column with thrombin diluted 1:250 in thrombin cleavage buffer (Novagen #69671-3). The thrombin solution was loaded manually into the column with a syringe ...
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bioRxiv - Cell Biology 2019Quote: ... the proteins were blotted onto a nitrocellulose membrane and probed with anti-GFP (1:1000; Sigma-Aldrich and Roche), anti-dgt6 (1:500 ...
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bioRxiv - Biochemistry 2019Quote: ... Proteins were resuspended in a solution containing 1 % SDC and 10 mM TCEP with 40 mM CAA (Sigma-Aldrich) as reduction and alkylating agents and heated to 37 °C for 1 hour ...
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bioRxiv - Cell Biology 2021Quote: Expression of Flag- and EGFP-tagged proteins was induced with media containing 1 µg/mL doxycycline (Sigma-Aldrich D9891) 12-24 hours prior to harvesting ...
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bioRxiv - Biochemistry 2020Quote: ... The protein was concentrated to 40 mg ml-1 with a centrifugal filter device (Millipore 50 kDa MW cutoff), and frozen until crystallization.
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bioRxiv - Neuroscience 2021Quote: ... the cells were incubated with the primary antibodies of interested proteins at 4°C overnight (Nestin, 1:1000, Sigma; Tuj1 ...
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bioRxiv - Biochemistry 2020Quote: ... Protein post cleavage was injected into FPLC and run over two HisTrap FF Crude 1 mL columns (Sigma-Aldrich) with FPLC running buffer ...
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bioRxiv - Biochemistry 2020Quote: ... NaCl was removed from the buffer and the protein was concentrated to 100 mg mL−1 using a 50,000 Da molecular mass cut-off concentrator (Millipore). The concentrated protein was incubated with a four-fold molar excess of dalbavancin at 4 °C for 12 h ...
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bioRxiv - Plant Biology 2020Quote: ... The protein extraction buffer (50 mM TrisHCl, pH 7.0, 1% w/v SDS, P9599 protease inhibitor cocktail, Sigma-Aldrich) was added to the powder ...
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bioRxiv - Systems Biology 2021Quote: ... separated proteins were transferred to a PVDF membrane using a semi-dry system and His-tagged proteins (hCDKL5 and His-Neuropilin) were detected with a HRP-conjugated anti-His antibody (1:2,000, Sigma) using the Enhanced Chemiluminescence kit (ECL ...
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bioRxiv - Microbiology 2021Quote: ... Antibodies to the N protein was detected by incubation for 1h at 37°C with anti-human IgG peroxidase (1:30,000, Sigma). The plates were washed three times with PBS with 0,05% Tween-20 between each step ...
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bioRxiv - Molecular Biology 2020Quote: ... Cells were blocked with 3% w/v BSA and incubated with 1:16,000 v/v rabbit anti-protein A antibody (SIGMA) for 1 h at room temperature ...
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bioRxiv - Cell Biology 2022Quote: ... The glass surfaces were coated with 1 mg/mL of BSA protein (Albumin, biotin-labeled bovine, A8549 Sigma-Aldrich) by incubating for 2 hours ...
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bioRxiv - Neuroscience 2022Quote: Mouse monoclonal Anti-Glial Fibrillary Acidic Protein (GFAP) antibody (diluted 1:200; cat. no. G3893; Sigma-Aldrich, MO, USA) and Goat anti-Mouse IgG (H+L ...
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bioRxiv - Physiology 2019Quote: ... FLAG-tagged protein were eluted twice with 200 ng/μL FLAG peptide (Sigma, #F3290; 1 h at 4°C). PL transfer activity was assayed using NBD labeled Phosphoethanolamine ...
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bioRxiv - Biochemistry 2019Quote: ... and proteins were resuspended in 100 μL of 100 mM ammonium acetate and 1 μg trypsin (Proteomics grade, Sigma). Proteins were digested by incubation at 37 °C for 16 h.
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bioRxiv - Developmental Biology 2019Quote: ... proteins were transferred to a PVDF membrane and immunoblotted with an anti-phosphotyrosine antibody (1:1000 dilution, Upstate/Millipore) for chemiluminescence detection.
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bioRxiv - Genetics 2021Quote: ... Reversal of TetR fusion protein binding was achieved by addition of 1 μg/ml Doxycycline (final concentration / Sigma-D9891) to mESC culture medium.
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bioRxiv - Biochemistry 2021Quote: ... FLAG-tagged L protein mutants were detected using peroxidase-conjugated anti-FLAG M2 antibody (1:10,000) (A8592; Sigma-Aldrich) and bands were visualized by chemiluminescence using Super Signal West Femto substrate (Thermo Scientific ...
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bioRxiv - Immunology 2021Quote: ... Efficiency of the expression and purification of recombinant proteins were determined by 15% SDS-PAGE and Western blotting by using anti-his tag antibodies (1:10,000) (Sigma). Protein concentrations were quantified by comparison with a curve of BSA resolved by SDS-PAGE.
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bioRxiv - Immunology 2021Quote: ... 1 mM EDTA) buffer and OAS1 proteins were immunoprecipitated from the lysate using 20 µg anti-FLAG antibody (Sigma) and Dynabeads Protein G ...
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bioRxiv - Genetics 2020Quote: ... Proteins were detected using either secondary anti-mouse or anti-rabbit IgG coupled to peroxidase (Millipore, 1:5000 dilution), western bright ECL substrate (Advansta ...
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bioRxiv - Biochemistry 2022Quote: ... 4 mM DTT and 5% D2O at a protein concentration 1 mM using a Shigemi NMR tube (Sigma Aldrich). Data was collected on an ultrahigh field Bruker 900 MHz NMR spectrometer equipped with a triple resonance cryogenic probe ...
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bioRxiv - Plant Biology 2022Quote: ... immunoblotting was performed with 50 μg of protein extracts using anti-Flag-M2-HRP (Sigma-Aldrich, A8592; 1:10,000), anti-c-myc-peoxidase (Roche ...
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bioRxiv - Physiology 2022Quote: ... 80 μg of protein were incubated in 10 mM L-cysteine and 1 mM pyridoxal 5′-phosphate hydrate (Sigma); or ...
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bioRxiv - Microbiology 2022Quote: ... Clarified lysates were incubated with 1.5 µg polyclonal anti-V5 or IgG for 1 h and captured with protein A agarose resin (P2545, Sigma) for 30 min ...
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bioRxiv - Molecular Biology 2022Quote: ... Nanobody fusion proteins were detected using a primary anti-HA (mouse; 1:3,000, Sigma-Aldrich, St. Louis, MO, USA). An anti-mouse IgG-horseradish peroxidase (HRP ...
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bioRxiv - Microbiology 2022Quote: Cleaved protein was concentrated to ~1 mL using an Amicon Ultra-15 centrifugal filter 30 kDa MWCO (EMD Millipore), then loaded onto a HiLoad Superdex S200 pg gel filtration column (GE Healthcare Life Science) ...
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bioRxiv - Microbiology 2024Quote: ... HA tagged proteins were detected in virus lysates using rabbit polyclonal anti-HA antibody (Cat# H6908, Sigma, 1:1,000). The V5 tagged proteins were detected in cell and virus lysates using anti-V5 mouse monoclonal antibody (Cat# V8012 ...
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bioRxiv - Microbiology 2024Quote: ... HA tagged proteins were detected in cell lysates using mouse monoclonal anti-HA antibody (Cat# H9658, Sigma, 1:10,000). HA tagged proteins were detected in virus lysates using rabbit polyclonal anti-HA antibody (Cat# H6908 ...
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bioRxiv - Evolutionary Biology 2024Quote: ... the equivalent of 500-800 µg of protein was incubated with 1 µg of anti-GFP antibody (Sigma-Aldrich) and then incubated with Protein A/G magnetic beads (Thermofisher ...
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bioRxiv - Microbiology 2024Quote: ... The purified protein was concentrated to ∼1 mg/mL by centrifugal filtration (AmiconTM 30K, Millipore Sigma, Burlington, MA, USA), flash frozen in liquid nitrogen ...