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Citations for Millipore Sigma :
2601 - 2650 of 10000+ citations for HLA A*0201 WT 1 complex Protein Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: ... for 3h at 16°C to remove the 6-His and MBP tag before phase separation in reaction mixtures in buffer C containing 10μM of WT or W1145R TopBP1b6-8-GFP and 2% of PEG4000 (Merck-Sigma-Aldrich, 95904). Reaction mixtures were mixed by gently tapping the Eppendorf ...
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bioRxiv - Cell Biology 2020Quote: U-2-OS Tet-On cell lines expressing eGFP-TopBP1 WT were grown under standard conditions (37°C, 5% CO2) in modified McCoy’s 5a medium (Merck-Sigma-Aldrich, M9309) supplemented with 10% fetal bovine serum (FBS) ...
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bioRxiv - Immunology 2020Quote: ... all CIS43 VLPs and wild-type (WT) Qβ VLPs were depleted of endotoxin by three rounds of phase separation using Triton X-114 (Sigma-Aldrich), as described in reference 48 ...
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bioRxiv - Molecular Biology 2021Quote: Mitochondrial NAD levels were quantified from three independent mitochondrial preparations from both WT and xrn1-H41A strains using NAD/NADH Quantitation Kit (Sigma-Aldrich). Mitochondrial extract corresponding to10 μg of protein from both WT and xrn1-H41A were used to determine NAD levels as per instructions provided in the kit.
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bioRxiv - Molecular Biology 2020Quote: ... or 1 mg of protein extract (for CSR-1 IPs comparing WT IP with catalytic mutant) was incubated with 15 µl of packed Anti-FLAG M2 Magnetic Agarose Beads (Sigma M8823) for 1 hour at 4°C ...
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bioRxiv - Developmental Biology 2022Quote: ... and 115% wt/vol) were generated by dissolving D-(-)-fructose (JT Baker) in Milli-Q water with 0.5% α-thioglycerol (Sigma-Aldrich) to prevent browning and 0.02% sodium azide to prevent fungal infection in the higher concentration solutions ...
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bioRxiv - Cell Biology 2021Quote: Osteoclasts plated on glass coverslips and bone slices for 3 days were fixed for 10 min in a 3.7% (wt/vol) paraformaldehyde (Sigma Aldrich 158127) solution containing 0.25% glutaraldehyde (Electron Microscopy Sciences 16220 ...
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bioRxiv - Microbiology 2020Quote: ... Apparent steady-state kinetic constants were determined by monitoring the WT and mutant EPSPS activities at varying concentrations of 2-phosphoenolpyruvate (PEP – Sigma-Aldrich), and fixed-saturating concentrations of shikimate-3-phosphate (S3P - Sigma-Aldrich ...
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bioRxiv - Cell Biology 2021Quote: ... WT and SIRT1 KO mESCS were treated with c-Myc Inhibitor CAS 403811-55-2–Calbiochem (10058-F4) (Millipore Sigma, 475956) at 10 μM or EZh2 inhibitor Tazemetostat (EPZ-6438 ...
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bioRxiv - Bioengineering 2022Quote: Films (N = 5 per condition) were lyophilized and incubated for 48 h in 5 wt% trichloroacetic acid (TCA, T6399, Sigma-Aldrich). Scaffolds (N = 5 per condition ...
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bioRxiv - Biochemistry 2022Quote: ... coli cells expressing either WT- or E14Q-EmrE constructs were grown in Mueller-Hinton broth (Sigma, 100µg/mL carbenicillin, pH 7.0) from a single colony to an OD of 0.2 at 37 °C ...
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bioRxiv - Cancer Biology 2022Quote: ... tumor tissue was minced and digested at 37°C for 12 h in a dissociation buffer containing 0.012% (wt./vol.) collagenase XI (Sigma-Aldrich, C7657) and 0.012% (wt./vol. ...
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bioRxiv - Neuroscience 2021Quote: ... tertiary neurospheres of WT and Tg mice were cultivated in the presence or absence of GCV (10 µM final concentration, Sigma G2536) for 2 weeks ...
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bioRxiv - Neuroscience 2020Quote: ... 10% (wt/vol) brain homogenate was prepared as reported above and mixed with a final concentration of 2% sarkosyl (Sigma Aldrich) and 0.5% benzonase (Sigma Aldrich ...
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bioRxiv - Systems Biology 2020Quote: Mammalian expression vectors encoding Flag-epitope tagged full-length Csk and eGFP-tagged Ptpn18 WT & Y/F mutant were transfected into 293T cells using 5 ug/mL PEI (Polyethylenimine, Sigma Aldrich) in Opti-MEM (GIBCO) ...
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bioRxiv - Molecular Biology 2020Quote: ... Cdh5-CreERT2xPnutsflox/flox (PNUTSEC-KO) and Pnutsflox/flox (WT) littermates were injected intraperitoneally with 2mg/day tamoxifen dissolved in peanut oil (Sigma-Aldrich) for 5 consecutive days and on the 8th and 10th day after the first injection ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: HEK 293 cells and WT or mutant A1R expressing HEK 293 cells were harvested using a non-enzymatic cell dissociation solution (Sigma-Aldrich) and washed with PBS prior to counting ...
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bioRxiv - Neuroscience 2019Quote: ... with the following modifications: 4-day-old female adult brains were dissected in PBS and then fixed in 4% (wt/vol) paraformaldehyde (Sigma-Aldrich) in PBS on ice for 20 min ...
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bioRxiv - Developmental Biology 2020Quote: WT and Gata5/6KD TgBAC (gata5: GFP)pd25 embryos were synchronized and dechorionated with pronase at 1mg/ml (Sigma, Cat# 11459643001) at desired stages ...
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bioRxiv - Immunology 2021Quote: ... iCD4TGFBR2 and WT C57Bl/6J mice were sensitized by i.p.-injections of 20 μg ovalbumin (OVA; grade V; Sigma-Aldrich, USA) in phosphate buffered saline (PBS ...
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bioRxiv - Physiology 2022Quote: The ovarian tissues of the mutants and WT control were homogenized and prepared by RIPA lysis buffer (EMD Millipore, Billerica, MA). The supernatant was collected ...
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bioRxiv - Biochemistry 2023Quote: ... Full-length BTK and BTK linker kinase domain (WT and mutants) were produced by co-expressing with YopH in BL21(DE3) (Millipore Sigma) or BL21-Gold(DE3 ...
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bioRxiv - Biophysics 2023Quote: ... HeLa WT and HeLa 43 were scrapes loaded with PBS containing 4 mg/ml Lucifer yellow CH (Sigma, Saint Louis, MO). After a 1-min incubation ...
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bioRxiv - Systems Biology 2023Quote: ... The bone sections were immediately sandwiched individually between two type B gold-coated copper freezer hats (BALTIC preparation, Wetter, Germany) with the addition of 10 wt% dextran (Sigma, 31390) as cryo-protectant ...
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bioRxiv - Neuroscience 2023Quote: ... Brains were then delipidated with clear unobstructed brain imaging cocktail and computational analysis (CUBIC)-L clearing solution (10 wt% N-buthyldiethanolamine [[#471240, Sigma-Aldrich, Germany] and 10 wt% Triton X-100 [Sigma-Aldrich ...
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bioRxiv - Microbiology 2023Quote: ... the WT and ΔfakA mutant were grown with fatty acid-containing and fatty acid-free HSA (Sigma-Aldrich, St. Louis, MO) at 10-40 mg/mL in TSB ...
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bioRxiv - Immunology 2023Quote: ... The pull-downed proteins were separated by SDS-PAGE on 10-to-20% gradient gel (ATTO) and the recombinant FLAG- tagged WT sCTLA-4 was detected by anti-FLAG M2 antibody (Sigma-Aldrich).
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bioRxiv - Cell Biology 2023Quote: ... RPE cells were harvested from WT and Akt2 KI mice and ChIP was performed using a commercially available kit (17-295, EMD Millipore). The following primers for the Atg9b promoter and negative control were used Atg9B_CHIP_F1 ...
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bioRxiv - Bioengineering 2023Quote: ... Then the samples were immersed in a pre-gel solution containing 30 wt % hydrogel monomers (N,N′-dimethylacrylamide; DMAA, Sigma-Aldrich) and 1 wt % Irgacure 2959 (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2023Quote: Testis samples whole testis samples from WT and spcar3 mice at 18 dpp (four biological replicates) were immersed in RNAlater (R0901, Sigma-Aldrich) until use ...
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bioRxiv - Pathology 2023Quote: ... Aortic samples were either snap-frozen in liquid nitrogen for bulk RNA sequencing or immersed in paraformaldehyde (4% wt/vol, P6148, Sigma-Aldrich) for histological analyses ...
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bioRxiv - Neuroscience 2023Quote: Mouse primary cortical neurons derived from Lrrk2 WT C57BL/6 and KO pups (P0) were fixed using 4% paraformaldehyde (PFA, Sigma-Aldrich) in PBS1X pH 7.4 for 20 minutes at room temperature and ...
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bioRxiv - Bioengineering 2023Quote: ... Brain slices with mesh probes modified with anti-D2R were placed in 1x PBS containing 4 % (wt/vol) acrylamide (Sigma-Aldrich) and 0.25 % (wt/vol ...
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bioRxiv - Bioengineering 2023Quote: ... and poly(acrylamide-co-acrylic acid) partial sodium salt (MW 150 kDa, acrylamide ∼80 wt. %, Cat. No. 511471) are purchased from Sigma-Aldrich, USA ...
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bioRxiv - Cell Biology 2024Quote: ... islets isolated from chow-fed C57BL/6 WT mice were pre-incubated in 20 mM cholesterol loaded onto methyl-β-cyclodextrin (MβCD) (C4951, Sigma Aldrich) diluted in 1X Krebs-Ringer bicarbonate-Hepes (KRBH ...
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bioRxiv - Cell Biology 2024Quote: ... coli according to standard methods (Brenner, 1974). Note that for one experiment (Fig. 4, wt;Levemisole 1mM) we added 10 mM levamisole (#Sigma T1512) to the petri dishes before pouring the (hand warm ...
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bioRxiv - Neuroscience 2024Quote: The electrolyte bath was comprised of an aqueous solution containing 2.5 mmol chloroplatinic acid (H2PtCl6, 8 wt% H2O, Sigma-Aldrich, 262587). The wafers were partially submerged in this solution using a custom-built wafer holder ...
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bioRxiv - Cell Biology 2024Quote: ... Expression of N-Flag WT or mutant TTC5 in rescue cell lines was detected by anti-Flag M2-HRP (Sigma A8592), monoclonal anti-Flag M2 (Sigma F3165) ...
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bioRxiv - Immunology 2024Quote: Eight-week-old WT and MCfosB- mice were sensitized with an intraperitoneal injection of 5 μg of anti-DNP IgE (SPE-7, Sigma-Aldrich) in 100 μL of sterile saline ...
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bioRxiv - Systems Biology 2021Quote: ... purified LC and naïve CD4+ T cells or TRMs were co-cultured in human serum supplemented media (RPMI, Gibco, UK, 10% human serum, Sigma, UK ...
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bioRxiv - Cancer Biology 2020Quote: Human neutrophils were obtained from fresh human peripheral blood from healthy volunteers and were isolated using a Percoll (Sigma-Aldrich) gradient.
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bioRxiv - Cell Biology 2022Quote: The human alveolar carcinoma epithelial cell line A549 (ATCC, CCL-185) and human colorectal adenocarcinoma cell line HT29 (Sigma Aldrich) were utilized for toxicological evaluation ...
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bioRxiv - Microbiology 2020Quote: ... 15 µl of secondary antibody (anti human Fab-HRP: Jackson ImmunoResearch, cat# 109-035-097; anti human IgG-HRP: Sigma, cat# A6029-1ML ...
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bioRxiv - Immunology 2022Quote: ... alpha enolase (in-house expressed human) and collagen II (bovine purified, chondrex) and at 0.5 μg/ml hIgG1 to citrullinated fibrinogen (human purifed, Sigma Aldrich).
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bioRxiv - Microbiology 2023Quote: Primary human macrophages (MΦs) were cultured in macrophage medium (RPMI-1640 GlutaMAX™ (Thermo) supplemented with 4% human AB serum (Sigma), 100 μg/ml penicillin-streptomycin (Sigma) ...
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bioRxiv - Physiology 2020Quote: ... diluted in 10 μL sterile phosphate-buffered saline (PBS) was mixed with 2.5 mg sucrose octasulfate-aluminum complex (Sigma-Aldrich Co., St. Louis, MO, USA), and 10 μL of 12% hydron in ethanol was added ...
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bioRxiv - Cell Biology 2019Quote: Protein carbonylation was determined by OxyBlot Protein Oxidation Detection Kit (Millipore). Wild-type animals were synchronized to the young adult stage ...
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bioRxiv - Cell Biology 2020Quote: ... Protein A-Sepharose and Protein G-Sepharose were purchased from Sigma-Alderich ...
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bioRxiv - Neuroscience 2019Quote: ... Protein concentration was determined using the bradford protein assay (Sigma-Aldrich). Proteins were separated in 8% SDS-polyacryamide gel (PAGE-SDS ...
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bioRxiv - Plant Biology 2021Quote: ... Protein blots were stained with Ponceau S reversible protein stain (Sigma) to check transfer efficiency and equal protein loading ...