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Citations for Millipore Sigma :
2551 - 2600 of 10000+ citations for HLA A*0201 WT 1 complex Protein Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2022Quote: ... rinsed with PBS and the proteins were visualized with Immobilon Western Chemiluminiscent HRP substrate (Merck/Millipore, used at 1:1:3 (H2O) dilution ...
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bioRxiv - Pathology 2020Quote: ... Proteins were immunoblotted with antibodies against FECH (rabbit, 1:400, LS Bio, #LSC409953, and β-actin (mouse, 1:5000, Sigma-Aldrich, AC40). Secondary antibodies ...
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bioRxiv - Microbiology 2021Quote: ... coverslips were labeled with antibodies as follows: HA-tagged proteins were detected with rat anti-HA 3F10 (Sigma 1:200-1:500), parasitophorous vacuole with in-house mouse anti-MAG1 (1:500) ...
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bioRxiv - Physiology 2021Quote: ... For colocalization 1:100 anti-NBCe1 was incubated with 1:1,000 anti-Clara Cell Secretory Protein (CCSP; Merck Millipore cat#07-623) or 1:200 anti-alpha tubulin (Santa Cruz cat#sc-5286 ...
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bioRxiv - Microbiology 2021Quote: ... were immunized intraperitoneally with 100 µl of a 1:1 emulsion containing 50 µg recombinant protein LukS or LukF and incomplete Freund’s adjuvant (Sigma-Aldrich, Taufkirchen, Germany). Mice were boosted with an emulsion of protein and incomplete Freund’s adjuvant at day 14 and 28 and bled after six weeks ...
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bioRxiv - Physiology 2024Quote: ... For colocalization 1:100 anti-CHRM3 was incubated with 1:500 anti-Clara Cell Secretory Protein (CCSP; Merck Millipore cat#07– 623), 1:200 anti-alpha tubulin (Santa Cruz ...
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bioRxiv - Microbiology 2023Quote: ... active and inactive NS2B(H)-NS3 pro proteases were generated as described previously and cloned into the MCS1 (multiple cloning site 1) of dual protein expression plasmid pETDuet-1 (Novagen, Beijing, China) through restriction sites EcoRI and NotI ...
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bioRxiv - Cell Biology 2021Quote: ... Proteins were transferred to a PVDF membrane and incubated in 1% Western blocking reagent (Sigma-Aldrich) at 25°C for 1 h ...
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bioRxiv - Molecular Biology 2021Quote: ... The following antibodies were used for the detection of protein expression: actin (1:1,000,Sigma, USA), angiogenin (Ang ...
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bioRxiv - Immunology 2019Quote: ... We prepared protein lysates by scraping the cells in cold lysis buffer containing 1% Igepal (Sigma) and CompleteTM protease inhibitor cocktail and preclearing by centrifugation at 14,000 g for 10 min at 4°C ...
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bioRxiv - Immunology 2019Quote: ... Primary antibodies used were: rabbit anti-prosurfactant protein C (AB3786, EMD Millipore, Burlington, MA; 1:1000), rabbit anti-keratin 14 (KRT14 ...
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Cdc42 promotes epithelial morphogenesis by coupling Par-complex and Crumbs recruitment via Par6-aPKCbioRxiv - Cell Biology 2019Quote: ... The following antibodies were used for protein detection: anti-Flag M2 mouse 1/1,000 (F3165, Sigma), anti-myc 9E10 mouse 1/1,000 (sc-40 ...
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bioRxiv - Molecular Biology 2021Quote: ... In order to reverse of TetR-CBX7 fusion protein binding 1 µg/ml Doxycycline (Sigma, D9891) was added to cell culture medium.
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bioRxiv - Genomics 2021Quote: ... Prior to protein quantification milk was diluted 1:2 in assay buffer (Merck-Millipore, Burlington, USA). Concentrations for 15 cytokines (IL-1α ...
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bioRxiv - Genetics 2020Quote: ... and diluted to measure protein concentration (1:10 diluted samples, Bradford Assay Kit, Sigma-Aldrich, B6916) and ATP level (1:2,000 diluted samples ...
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bioRxiv - Cell Biology 2021Quote: ... and bound proteins were eluted with 25 µl 1 mg/ml 3 × FLAG peptide (Sigma, F4799) in IP buffer containing 1% NP-40 and 1 × protease inhibitors at 4 °C ...
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bioRxiv - Plant Biology 2020Quote: ... 2 mM EDTA and 1 % [v/v] protein phosphatase inhibitor cocktail 2 and 3 (Sigma-Aldrich)) were added at a concentration of 2 mL/g tissue powder ...
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bioRxiv - Biochemistry 2022Quote: ... The primary antibodies used were: mouse monoclonal anti-glial fibrillary acidic protein (GFAP) (1:400; Sigma) and goat polyclonal anti-ChaT (1:100 ...
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bioRxiv - Biophysics 2022Quote: ... Five microliters of a 5 mg mL−1 solution of protein G (Sigma-Aldrich, Tokyo, Japan) was added to the flow chamber (about 5 μL) ...
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bioRxiv - Neuroscience 2020Quote: ... Purified protein was then eluted by HPDP reduction with 1% (v/v) β-mercaptoethanol (Millipore-Sigma) in elution buffer (0.2% [w/v] SDS and 0.25M NaCl in dilution buffer ...
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bioRxiv - Neuroscience 2020Quote: ... Purified protein was then eluted by HPDP reduction with 1% (v/v) β-mercaptoethanol (Millipore-Sigma) in elution buffer (0.2% [w/v] SDS and 0.25M NaCl in dilution buffer ...
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bioRxiv - Neuroscience 2020Quote: ... and mouse monoclonal IgG2A antibody to myelin Proteolipid Protein (clone PLPC1, MAB388, MERCK-Millipore, 1:100). All slides were mounted using Mowiol mounting medium (475904 ...
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bioRxiv - Biochemistry 2021Quote: ... Protein production was induced by adding 1 mM IPTG and 10 µM all-trans retinal (Sigma) at an absorbance of 0.8–0.9 at 600 nm ...
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bioRxiv - Genetics 2022Quote: ... hPSC-CMs were stained with sarcomreirc protein TNNT2 (Catalog #: HPA017888 Lot #: A91786, Sigma, dilution 1/200), secondary antibody Goat anti-Rabbit IgG Alexa Fluor 568 (Invitrogen ...
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bioRxiv - Biochemistry 2019Quote: ... Proteins synthesized in this assay were detected using anti-FLAG antibodies (F3165 SIGMA, 1:4000 dilution) and the Anti-mouse IgG ...
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bioRxiv - Physiology 2019Quote: ... was from BD Biosciences; citrate synthase (CS; SAB2701077) and mitochondrial fission protein 1 (FIS1; HPA017430) were from Sigma Aldrich; vitamin D receptor (D-6 ...
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bioRxiv - Molecular Biology 2021Quote: ... Protein expression was induced by adding 1 mM of isopropyl-β-D-thiogalactoside (IPTG) (Sigma Aldrich). Expression was continued in a bacterial shaker at 30°C and 150 rpm for 6h ...
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bioRxiv - Microbiology 2020Quote: ... HA-tagged proteins were detected by anti-HA mAb (clone HA-7, 1:5000, Sigma-Aldrich); NSP5 and VP2 proteins were detected using anti-NSP5 guinea pig serum (1:5000 ...
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bioRxiv - Neuroscience 2019Quote: ... Primary antibodies against glial fibrillary acidic protein (GFAP; 1:400; cat. no. Mab360; Millipore, Burlington, MA) and IBA-1 (1:500 ...
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bioRxiv - Cell Biology 2020Quote: ... the culture medium was supplemented with 1 μM Rho-associated protein kinase (ROCK) inhibitor (Y27632, Millipore). Recombination was induced with 100 nM 4-hydroxytamoxifen (4OHT ...
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bioRxiv - Cell Biology 2021Quote: ... 30 μg of proteins were mixed with 0.004% Bromophenol blue and 1% β-Mercaptoethanol (Sigma-Aldrich), incubated at 95°C for 5 min ...
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bioRxiv - Molecular Biology 2021Quote: ... purified proteins were eluted twice with 50 ul of 1 mg/ml 3X flag peptide (Sigma) in co-IP buffer on the nutator for 10 minutes at room temperature.
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Reducing mitochondrial ribosomal gene expression does not alter metabolic health or lifespan in micebioRxiv - Cell Biology 2022Quote: ... 140μg of mitochondrial protein was incubated with 1:100 of 1M dithiothreitol (Sigma-Aldrich, SKU 43815), followed by 1:50 dark incubation in 1M iodoacetamide (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2022Quote: ... The proteins were diluted 1:3 with Urea-buffer (4.5 mM Urea (Sigma-Aldrich Cat#U1250), 10 mM Tris-HCl ...
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bioRxiv - Biophysics 2024Quote: ... and when necessary concentrated the protein to 1∼2 mg/ml (Amicon Ultra 100 KDa, Millipore). For experiments under K+-free conditions ...
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bioRxiv - Bioengineering 2023Quote: ... with the protein aqueous solution (1 mg/ml of PLL, Sigma-Aldrich, in PBS pH 10.4), at a 1:2 ratio ...
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bioRxiv - Neuroscience 2023Quote: ... and bands were normalized to total protein and the housekeeping gene Gapdh (1:10,000, Millipore, NP_002037).).
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bioRxiv - Pathology 2023Quote: ... Proteins were detected with the following primary antibodies: mouse monoclonal anti-CnB (Sigma, 0581, 1:1000), rabbit polyclonal anti-CnA (Merck Millipore ...
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bioRxiv - Microbiology 2023Quote: ... the pellets were washed first with 1× BugBuster® (10× Protein Extraction Reagent Novagen® Merck), then twice with water and once with 50 mM NaH2PO4 (pH 8) ...
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bioRxiv - Cell Biology 2023Quote: ... 35 μg of proteins were supplemented with 0.004% Bromophenol blue and 1% β-mercaptoethanol (Sigma-Aldrich), incubated at 95 °C for 5 min ...
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bioRxiv - Biophysics 2023Quote: ... The eluted proteins were concentrated to 1 ml using Amicon Ultra-15 10k-cutoff filter (Millipore) and loaded onto a HiLoad Superdex 200 prep grade column (GE Healthcare ...
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bioRxiv - Biophysics 2023Quote: ... The eluted proteins were concentrated to 1 ml using Amicon Ultra-15 3k-cutoff filter (Millipore) and loaded onto a HiLoad Superdex 75 prep grade column (GE Healthcare ...
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bioRxiv - Biochemistry 2024Quote: ... and protein expression was induced with 0.5 mM isopropyl β-D-1-thiogalactopyranoside (IPTG) (Sigma 16758) at an OD of 0.5 ...
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bioRxiv - Cancer Biology 2024Quote: ... Antibodies to the following proteins were used in the immunoblots: puromycin (Millipore, #AF488, 1:2000; RRID:AB_2737590), 4E-BP1 (Cell Signaling ...
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bioRxiv - Immunology 2024Quote: ... recipient mice were intraperitoneally (i.p) injected with 50µg of OVA protein (Sigma Aldrich, 9006-59-1). On day three following cell transfer ...
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bioRxiv - Molecular Biology 2024Quote: ... Protein expression was induced by adding 0.25 mM isopropyl β-D-1-thiogalactopyranoside (IPTG; [Sigma-Aldrich]) and bacterial cultures were grown at 18 °C for 20 hours ...
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bioRxiv - Plant Biology 2024Quote: ... SnRK2s-GFP as well as phosphorylated HUB2 proteins by using anti-PAP (1:1000, SIGMA, P1291), anti-GFP (1:2000 ...
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bioRxiv - Microbiology 2024Quote: ... supplemented with 1% FBS (v/v) and protein expression enhancing sodium butyrate (66) solution (SIGMA-ALDRICH) to a final concentration of 2 nM were added to the cells to make up to the final volume of transfection ...
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bioRxiv - Immunology 2021Quote: ... Lysates of ФNX cells transfected with WT of pcDNA3-FLAG-PARP14 were either analyzed directly or used for immune precipitations with monoclonal anti-FLAG (M2) (Sigma-Aldrich). The resulting immune complexes ...
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bioRxiv - Cell Biology 2019Quote: ... and 2 mL of imaging buffer with an oxygen scavenging system56 (50 mM Tris-HCl pH 8.0, 10% wt/v glucose, 2 mM Trolox (Sigma-Aldrich, 238813), 0.5 mg/mL glucose oxidase (Sigma-Aldrich ...