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Citations for Millipore Sigma :
2601 - 2650 of 3014 citations for 6 METHYLFLAVONE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... counter-stained with 4’,6-diamidino-2-phenylindole (DAPI) for 5 minutes at room temperature and mounted with Fluoroshield (Sigma-Aldrich).
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bioRxiv - Molecular Biology 2022Quote: ... The cells were washed in PBS and stained with 5 µg/ml 4′,6-Diamidino-2- phenylindole (DAPI) (D9564-10MG, Sigma-Aldrich) in 1xPBS+0.1% Triton X-100 (Sigma-Aldrich ...
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bioRxiv - Microbiology 2022Quote: ... cells seeded in 12-well plates were pre-treated with 500 mU/mL α2-3/6/8 sialidase from Clostridium perfringens (Sigma-Aldrich) for 3 hours at 37°C before infection ...
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bioRxiv - Microbiology 2022Quote: ... were place on the plate and 10 μL of (1, 2, 4 and 9.8 M) H2O2 and (2, 4, 6 M) methyl viologen (Sigma-Aldrich, UK) was dispensed onto each separate disk ...
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bioRxiv - Neuroscience 2022Quote: ... Cells were plated in 24-,12- or 6-well plates pre-coated with 10 μg/ml of poly-L-lysine (Sigma-Aldrich) and maintained at 37°C in a humidified atmosphere containing 5% CO2.
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bioRxiv - Neuroscience 2022Quote: HEK293 cells stably expressing FLAG-tagged D3R were grown in DMEM with 10% FBS in 6-well plates coated with 400 µl ECM (Sigma E1270) to 80-90% confluence ...
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bioRxiv - Neuroscience 2022Quote: Embryos and larvae obtained from TL crosses were raised to the desired ages (30 hpf, 48 hpf, 6 dpf) and stored in RNAlater (Sigma, #MKCL5657). Total RNA was extracted from 10 dissected eyes and 10 dissected heads without eyes at each age using a phenol chloroform extraction method with TRI-Reagent (Invitrogen) ...
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bioRxiv - Neuroscience 2022Quote: ... 6; MgCl2, 4; CaCl2, 5; sucrose, 160; glucose, 25; Hepes, 10; pH 6.7, 500 mOsmol; all chemicals from Sigma-Aldrich, France), to avoid desiccation of the brain surface ...
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bioRxiv - Physiology 2023Quote: ... the cell culture medium from 6-well plates was collected and either concentrated with Amicon Ultra microconcentrator 10 kDa cut-off (Merck Millipore #UFC501096) or not ...
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bioRxiv - Physiology 2023Quote: ... the cell culture medium from 6-well plate was collected and either concentrated with Amicon Ultra microconcentrator 10 kDa cut-off (Merck Millipore #UFC501096) or not ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Breeding pairs of all mice strains (6 weeks old) were fed with a chow diet containing 0.012% TCS (Sigma-Aldrich, 72779) dissolved in vehicle (2ml DMSO + 10ml water ...
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bioRxiv - Molecular Biology 2022Quote: ... dechorionated embryos were crosslinked in a mixture of 2 ml fixation buffer (PBS, 0.5% Triton X-100) and 6 ml heptane supplemented with 100 μl of 37% formaldehyde (Sigma-Aldrich, F8775) for 15 min at room temperature with rotation ...
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bioRxiv - Neuroscience 2022Quote: ... or the same volume of vehicle containing ghrelin receptor antagonist (GRA; [D-Lys3]-GHRP-6; 3.3mg/kg BW; Sigma-Aldrich G4535). This GRA dose was based on our previous report demonstrating its ability to restore cholecystokinin-induced NTS neuronal activation in food deprived rats [20] ...
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bioRxiv - Immunology 2022Quote: ... After washed with PBS for 3 times and stained with DAPI (4’,6-diamidino-2-phenylindole) (50 ng/mL) (Sigma, USA) for 5 min ...
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bioRxiv - Systems Biology 2022Quote: ... cells were washed with PBS and their nuclei were stained with 200 ng/mL 4’,6-diamidino-2-phenylindole (DAPI) (Sigma-Aldrich) for 10 min ...
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bioRxiv - Bioengineering 2022Quote: ... cell-laden microgels were demulsified and resuspended by an excessive amount of LB media spiked with 10−6 mol/L 3-Oxo-C12-HSL (Sigma-Aldrich) before incubation at 37 °C ...
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bioRxiv - Neuroscience 2022Quote: ... After the addition of the appropriate amount of the 6× Laemmli Sample Buffer (Bioland scientific LLC, sab03-02) with 5% ß-mercaptoethanol (Sigma) protein samples (10-30 μg ...
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bioRxiv - Cell Biology 2022Quote: ... human very-low-density and low-density lipoproteins (catalog no. 437647-5MG and 6 mg/ml, LP2-2MG, Merck Millipore, respectively) were purchased and stored according to the manufacturers’ instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... mice were randomized (n=6) and treated with vehicle (10% D-a-tocopherol polyethylene glycol 1000 succinate in PBS, Sigma Aldrich) or VE822 (60 mg/kg ...
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bioRxiv - Cell Biology 2023Quote: ... Dissociated neurons were then resuspended in warm DMEM supplemented with 10% FBS and plated in 6-well plates containing 25-mm sonicated glass coverslips pretreated with 50 μg/ml poly-L-lysine (PLL, Sigma-Aldrich). After 1 hour ...
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bioRxiv - Cancer Biology 2024Quote: ... Pancreas-specific Cre recombinase activity was induced in 6-week-old mice through the administration of tamoxifen (Sigma-Aldrich, cat. # T5648) daily for 6 consecutive days at a dose of 0.15 mg/g body weight followed by rest for a week before onset of ACP induction phase.
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bioRxiv - Neuroscience 2023Quote: ... The cells were then centrifuged at 200 rcf for 5 min and resuspended at a density of 2×105 cells/ml in ultra-low attachment 6-well plates (Sigma, CLS3471) using N2B27 medium (50% Advanced DMEM/F12 (ThermoFisher ...
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bioRxiv - Physiology 2024Quote: ... KD-LX2 and Scr-LX2 cells were seeded in 6-well-plates (4x104 cells/well) for 48hrs before being stained with Nile Red (Sigma-Aldrich) and evaluated with densitometry by ImageJ software to measure lipid content [24] ...
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bioRxiv - Molecular Biology 2024Quote: ... and incubated in blocking buffer containing secondary antibodies and 0.5 μg/mL 4’,6-diamidino-2-phenylindole (DAPI; Sigma-Aldrich #D9542) for one hour ...
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bioRxiv - Immunology 2024Quote: Bone marrow-derived macrophages were prepared from C57BL/6 and Rel-/-mice as described.68 Macrophages were activated on day 6 with lipid A for the time indicated in each experiment (Sigma-Aldrich). Nascent transcript RNA-seq was performed and the data were analyzed as described.68,69
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bioRxiv - Neuroscience 2024Quote: ... Slides containing the sections were immersed for 40 min in 10 mM citrate buffer pH 6 (sodium citrate, Sigma Aldrich, Germany) at 96°C in a water bath ...
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bioRxiv - Microbiology 2024Quote: ... approximately 7×105 cells growing on 6-well plates were washed with PBS and harvested with Tri Reagent (Sigma Cat#T9424). Total RNA was extracted ...
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bioRxiv - Cancer Biology 2024Quote: 0.5 to 1 million cells were plated into 6-well plate and treated with 0.5 or 1 mg/ml Dox (Sigma-Aldrich #D9891) to induce FGFR4 overexpression or knockdown for 48 hours ...
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bioRxiv - Developmental Biology 2023Quote: ... mESCs were cultured in feeder-free conditions on tissue-culture treated 6-well plates (Falcon® 353046) coated with 0.1% gelatine (Sigma G1393), in KnockOut DMEM (Gibco™ 10829018 ...
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bioRxiv - Neuroscience 2024Quote: ... the cells were fixed with 4% paraformaldehyde for 10 min and treated with 4’,6-diamidino-2-phenylindole (DAPI; Sigma-Aldrich) diluted to a 1/10,000 concentration from the stock solution ...
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bioRxiv - Neuroscience 2024Quote: Mice 4-6 weeks old were given three doses 150 mg/kg or 200 mg/kg (i.p) tamoxifen (TAM) (Sigma-Aldrich T5648) dissolved in peanut oil (20 mg/ml ...
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bioRxiv - Microbiology 2024Quote: ... with the same combination of primary and secondary antibodies: mouse anti-acetyl-alpha tubulin antibody clone 6-11-B1 (Sigma⍰Aldrich) at a 1:100 dilution and rabbit anti-HA tag antibody (Cell Signalling Technology ...
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bioRxiv - Microbiology 2024Quote: ... The following mixture of primary antibodies was used: a mouse anti-acetyl-alpha tubulin antibody clone 6-11-B1 (Sigma⍰Aldrich) at a 1:100 dilution and a rabbit anti-HA tag antibody at a 1:50 dilution (Cell Signalling Technology) ...
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bioRxiv - Cell Biology 2023Quote: ... and postfixed with 4% cold PFA for 10 min followed by permeabilization in 0.5% Triton X-100/PBS for 3 h at room temperature (20–25°C) and incubation with 2 µg ml-1 4′,6-diamino-2-phenylindole (DAPI; D9542, Sigma-Aldrich) for 30 min ...
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bioRxiv - Cell Biology 2024Quote: ... virus was collected and used to infect NIH3T3 cells with 6 µg/ml polybrene (Sigma, St Louis, MO, USA; #TR-1003) for another 12 h ...
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bioRxiv - Developmental Biology 2024Quote: ... Cochlear explants were cultured for 6 days in DMEM with 10% fetal bovine serum and 10 µg/ml ciprofloxacin (Sigma, 17850). Explants were then fixed in 4% PFA for 15 min at room temperature before being processed for immunolabeling.
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bioRxiv - Plant Biology 2024Quote: Flowers at position 6 were gently incubated in staining solution (25 µM (2’,7’-dichlorodihydrofluorescein diacetate) (H2DCF-DA) (Sigma-Aldrich, D6883), 50 mM KCL ...
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bioRxiv - Physiology 2024Quote: ... subcutaneous white adipose tissue (sWAT) from male C57BL/6 mice was digested with 1 mg/mL type 2 collagenase (Sigma Aldrich) at 37°C for 40 minutes with shaking ...
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bioRxiv - Molecular Biology 2024Quote: ... for differentiation induction 100.000 cells were plated in a 6-well plate and maintained in complete media supplemented with 20μM retinoic acid (Sigma-Aldrich, R2625) in dimethyl sulfoxide (DMSO ...
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bioRxiv - Cell Biology 2024Quote: MSCs were seeded overnight on 24 or 6 well plates in normal α-MEM supplemented with 10% fetal bovine serum (FBS, Sigma), 2 mM glutamine ...
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bioRxiv - Developmental Biology 2024Quote: ... ovaries were washed for 1 h with PBX and stained with 1 μg/ml of 4′,6-diamidino-2-phenylindole (DAPI) (SIGMA, USA) for 10 min in PBX ...
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bioRxiv - Biochemistry 2024Quote: ... The HeLa cells were transduced in 6-well plates with 1 mL of unconcentrated virus + 10 µg/mL polybrene (TR-1003-G, Sigma Millipore) per well for 24 h ...
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bioRxiv - Bioengineering 2024Quote: ... The samples were then washed three times in PBS and incubated with 4’-6-diamidino-2-phenylindole (DAPI) (1:1000; Sigma-Aldrich) at room temperature for 20 min ...
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bioRxiv - Biochemistry 2024Quote: ... mice were fasted for 6 hours (for GTT) or 5 hours (for ITT) and received an intraperitoneal injection of glucose (1g/kg, Sigma Aldrich) or insulin (0.75 units/kg ...
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bioRxiv - Biophysics 2023Quote: Mechanically passaged hiPSCS were plated on 10 µg/mL Vitronectin XF in 6 well TC plates in 1 μM all-trans-RA (Cat. No. R2500-25MG, Sigma-Aldrich) to NutriStem® hPSC XF Medium (Growth Factor-Free)(Cat ...
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bioRxiv - Cell Biology 2024Quote: ... GV oocytes were collected from the ovary of 3-4 weeks old C57BL/6 females 48 h after PMSG injection and then were incubated in M2 medium (Sigma, #M7167) supplemented with 10 μM Cilostamide (TargetMol ...
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bioRxiv - Bioengineering 2024Quote: ... The immortalized Podo/Tert256 human podocyte hTERT cell line (Evercyte) was expanded in flasks coated with 6 µg/cm2 human collagen I (Sigma, C7774) at 37°C in MCDB131 basal media (Gibco ...
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bioRxiv - Immunology 2022Quote: 5–7-weeks old C57BL/6 mice bearing GL261 or CT-2A tumors were treated with KDM6B inhibitor (GSK-J4; Sigma-Aldrich); 1mg per mouse ...
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bioRxiv - Neuroscience 2023Quote: ... The spinal column was removed and 4-6 lumbar level DRG were dissected and placed onto coverslips coated with Poly-L-Lysine (Sigma, P8920). DRG were allowed to recover for 8 minutes (P4 ...
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bioRxiv - Neuroscience 2023Quote: ... Western Blotting experiments on viral particles were carried out on the collected culture media concentrated with Vivaspin 6 MWCO 30 kDa membranes (Sigma Aldrich). Samples were then reduced and denatured by boiling in 4X Laemmli sample buffer at 95°C for 5 minutes ...