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Citations for Millipore Sigma :
2551 - 2600 of 3014 citations for 6 METHYLFLAVONE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: ... miR-511-3p transfected cells as well as controls were stimulated on day 6 with RSG in the presence or absence of LPS (Sigma Aldrich) for 24 hours before harvesting ...
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bioRxiv - Molecular Biology 2020Quote: For lentiviral transduction 200.000-250.000 ES cells per well were seeded on gelatinized 6-well dishes in ES medium containing 8 μg/ml polybrene (Sigma, #H9268). Viral supernatant was added to the cells and plates were spun in a prewarmed centrifuge (Heraeus Multifuge 4KR ...
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bioRxiv - Molecular Biology 2020Quote: ... To stabilize beta-catenin and to induce canonical Wnt signaling the cells were treated with 1 μM BIO (6-Bromoindirubin-3′-oxime, SIGMA B1686) for 24 hours before harvesting.
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bioRxiv - Immunology 2020Quote: ... Membranes were then thoroughly washed (6 × 10 min) with PBS-T before revealed with SigmaFast NBT/BCIP substrate solution (Sigma-Aldrich).
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bioRxiv - Molecular Biology 2020Quote: ... The remaining 2 ml were added to falcon tubes containing 4 ml of 6 M GndHCl (Sigma Aldrich, Saint Louis, Missouri) and mixed thoroughly to denature the samples ...
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bioRxiv - Plant Biology 2020Quote: ... 5 μl PCR product for each barcoded sample was mixed with 5 μl 6×Orange G DNA Loading Dye (Orange G, 6X, Sigma, Germany) and run on a 1 % agarose gel in TAE 1X buffer ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were washed again for three times before the final staining of the nuclei using 4’,6-diamidin-2-phenylindol (DAPI, Sigma-Aldrich). Afterwards ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... cells were passaged to single cell suspension and plated to achieve 65,000 cells per well of a 6-well plate in Nutristem supplemented with 1:2,000 Y-27632 (ROCK inhibitor; Sigma Aldrich, Y0503) [23] ...
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bioRxiv - Neuroscience 2021Quote: ... The ΔNt17-Httex1 (6-42Q) proteins were dissolved in 10 mM PBS and 1 mM Tris (2-carboxyethyl)phosphine (TCEP, Sigma–Aldrich) to keep the N-terminal cysteine reduced.
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bioRxiv - Neuroscience 2020Quote: ... To identify the total number of cells the nuclei were stained with DAPI (4’, 6-Diamino-2-phenylindole dihydrochloride) (1:10000, Millipore-Sigma). Images were acquired using Olympus BX61 microscope and analyzed using Fiji software (ImageJ).
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bioRxiv - Neuroscience 2020Quote: ... The cells were centrifuged for 5 min at 700g and treated for 10 min with DAPI (4’,6-diamidino-2-phenylindole) (1:4000, Sigma, 62248) to label dead cells ...
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bioRxiv - Genomics 2020Quote: ... transfected in the Lonza 4D nucleofector program ‘CA-137’and seeded onto a Matrigel-covered 6-well plate in 3 ml StemFlex containing 10 μM Thiazovivin (Millipore, #S1459). The cells were subjected to a 48 hour cold shock at 32 °C to enhance homology directed repair (HDR) ...
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bioRxiv - Genomics 2020Quote: ... transfected in the Lonza 4D nucleofector program ‘CA-137’and seeded onto a Matrigel-covered 6-well plate in StemFlex containing 1 μM H1152 (Millipore, #555550). On the next day ...
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bioRxiv - Microbiology 2021Quote: ... Samples were then dried in 80% ethanol and mount on a glass slide with antifadent AF1 (Citifluor, USA) added with 4’-6’-diamidino-2-phenylindole (DAPI) (Sigma Aldrich) for counterstain at a final concentration of 2 μg.ml−1 ...
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bioRxiv - Microbiology 2021Quote: ... The supernatant was discarded and the pellet resuspended in 1 ml of BHI containing 10 µM 5(6)-carboxyfluorescein diacetate N-succinimidyl ester (CFDA-SE, Sigma #21888). The suspension was incubated on a rotating shaker at 37°C and 200 rpm for 45 minutes ...
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bioRxiv - Bioengineering 2020Quote: ... However initial mineralized collagen suspensions were created using one of three potential glycosaminoglycans: chondroitin-6-sulfate (Chondroitin sulfate sodium salt from shark cartilage, Sigma-Aldrich), chondroitin-4-sulfate (Sodium Chondroitin Sulfate A ...
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bioRxiv - Immunology 2021Quote: ... Plates were washed 6 times with 225 µL ELISA wash buffer and 1:5000 goat anti-human IgG HRP (Millipore Sigma) or anti-human IgG isotype specific HRP (Southern Biotech ...
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bioRxiv - Synthetic Biology 2020Quote: ... lentivirus was added to cells at a multiplicity of infection of 10–50 in the presence of 6 mg/ml of DEAE-dextran (Sigma Aldrich). After 18 hours ...
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bioRxiv - Bioengineering 2019Quote: ... HUVECs (12×106 cells/mL) and MSCs (6×105 cells/mL) were suspended in EBM-2MV supplemented with thrombin (4 U/mL) (Sigma-Aldrich). A solution of fibrinogen (8 mg/mL ...
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bioRxiv - Cell Biology 2020Quote: ... C9H2Cl4O2 was purchased from Tebu-Bio and used at 2μM working concentration. Monastrol (Ref. M8515) and 4′,6-Diamidino-2-phenylindole dihydrochloride (DAPI) (Ref. D8417) were purchased from Sigma-Aldrich. S-Trityl-L-cysteine (STLC) ...
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bioRxiv - Biochemistry 2020Quote: ... the acid insoluble fraction was incubated for 18 hours at 4°C with 500 μL of 6 M guanidine hydrochloride/100mM TRIS buffer (pH 7.4, Sigma-Aldrich, U.K.). The buffer was exchanged into 100 μL of 50 mM ammonium acetate (Scientific Laboratory Supplies ...
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bioRxiv - Physiology 2020Quote: ... On day 4 cells were re-stimulated with the same concentrations of anti-CD3/CD28 for 6 hours at 37°C in the presence of 1 μg/ml brefeldin-A (Sigma-Aldrich). As a control ...
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bioRxiv - Molecular Biology 2021Quote: ... Cryosections were then counterstained (5 min) with 0.5 µg/ml 4’,6’-diamino-2-phenylindole (DAPI; Sigma-Aldrich® Cat#D9542) in PBS ...
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bioRxiv - Neuroscience 2021Quote: ... Mice were habituated for 6 days to our animal facility before starting treatment with p-Cresol (reference W233706-SAMPLE-K, Sigma-Aldrich) dispensed in sterile drinking water at a concentration of 0.25 g/L ...
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bioRxiv - Molecular Biology 2021Quote: FSHD immortalized myoblast cells were seeded in 6-well plates pre-coated with extracellular matrix gel from Engelbreth-Holm-Swarm murine sarcoma (Sigma, UK). Following transfection with PMOs ...
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bioRxiv - Biochemistry 2021Quote: Stem sections were cut by hand from the basal third of stems of 6-week-old plants and were stained in 0.01% toluidine blue O (Sigma-Aldrich; T3260) for 2 min ...
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bioRxiv - Cancer Biology 2021Quote: ... each well of 6 well-plate were transferred to a 10cm plate with previously determined concentrations of puromycin (Sigma-Aldrich, USA), for that cell line for 3 days ...
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bioRxiv - Cell Biology 2021Quote: ... The supernatants were then used to quantify the level of adiponectin using Mouse Adiponectin ELISA kit (Merck Millipore #EZMADP-6 K) according to the manufacturer’s instructions.
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bioRxiv - Neuroscience 2021Quote: ... on 6 male Wistar (250–400 g; Charles Rivers) by a single intraperitoneal (IP) injection of pilocarpine (320 mg/kg; Sigma-Aldrich), one week after receiving the animals from the vendor ...
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bioRxiv - Cancer Biology 2020Quote: ... 1mL of Cas9 viral supernatant was added to 1×106 cells growing in a 6-well plate plate in 3mL of complete media with polybrene (4mg/mL) (Sigma, H9268). The cell-virus mixture was centrifuged at 3000rpm at 25°C for 1h followed by incubation at 37°C overnight ...
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bioRxiv - Cancer Biology 2021Quote: ... and subsequently transduced twice with viral supernatant within 48 hrs in the presence of 50 IU/mL IL-2 and 6 mg/mL polybrene (Sigma-Aldrich). TCR-transduced T cells were expanded by stimulation with anti-CD3/CD28 Dynabeads (500,000 beads/106 cells ...
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bioRxiv - Developmental Biology 2021Quote: ... 5×104 cells were seeded onto ultra-low adherence 6-well culture plates in 2 mL of serum-free DMEM-F12 media (Sigma-Aldrich), supplemented with 10 µg/mL Putrescine ...
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bioRxiv - Cell Biology 2020Quote: ... The pellet was suspended in 10 ml of Histopaque 1100 (5 ml histopaque 1077; Sigma 10771 and 6 ml histopaque 1119; Sigma 11191), centrifuged for 20 minutes at 1500 rpm at 4°C and the pellet washed 2 times with QB ...
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bioRxiv - Cell Biology 2020Quote: CFs subjected to transfection with NT or anti-Col 6 siRNA were incubated for 10 min on ice in RIPA Buffer (Sigma-Aldrich). Samples were then centrifuged at 13,000xg for 15 minutes and supernatants were collected ...
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bioRxiv - Genetics 2021Quote: ... The reaction was performed in the presence and absence of an NADPH generating system: 1 mM glucose-6-phosphate (Sigma Aldrich), 0.1 mM NADP+ (Sigma Aldrich) ...
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bioRxiv - Microbiology 2020Quote: ... which was added to the growth media from an Fe(III)-citrate solution prepared by mixing Fe(III)Cl3 (6 mM) and citric acid (12 mM) powders (Sigma-Aldrich) in Milli-Q water ...
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bioRxiv - Neuroscience 2020Quote: ... a single organoid per well was transferred on 6 well plates on day 77 and 120 in Diff2 medium with 20 ng/ml LPS serotype O111:B4 (Sigma # L2630) and incubated for 24 h ...
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Development of follicular dendritic cells in lymph nodes depends on retinoic acid mediated signalingbioRxiv - Immunology 2020Quote: ... cells were cultured for 6 hrs either in medium alone or in the presence of retinoic acid (100 nM, Fluka, Sigma-Aldrich, Zwijndrecht ...
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bioRxiv - Immunology 2021Quote: ... cells were incubated for 20 minutes at room temperature with DAPI (4',6-diamidino-2-phenylindole; 1:150000, Sigma-Aldrich), mounted on glass slides with Fluoromount-G (Invitrogen ...
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bioRxiv - Cell Biology 2021Quote: ... each piece of frozen tissue was placed into one well of a 6-well plate with salt-Tris buffer containing 146 mM NaCl (Cat#S6546-1L, Sigma-Aldrich), 1 mM CaCl2 (Cat#97062-820 ...
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bioRxiv - Cell Biology 2021Quote: ... glass slides were subjected to Prussian Blue staining using a 1:1 solution (v/v) of 10% K4[Fe(CN)6] (Sigma-Aldrich) and 20% HCl for 20 min ...
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bioRxiv - Biochemistry 2022Quote: ... Sample was then concentrated 6 times by ultrafiltration using a 3 kDa molecular weight cut off Centricon centrifugal filter units (EMD Millipore) and applied onto a Superdex 200 10/300 increase (GE Healthcare ...
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bioRxiv - Bioengineering 2022Quote: ... as previously described.23 The isolated tissues were incubated in cell dissociation solution comprising of 6 mg papain (P4762, Sigma, USA) in 3 ml of Hibernate E-Ca (HE-Ca ...
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bioRxiv - Cancer Biology 2022Quote: ... TILs and CAR-TILs were co-cultured with cancer cells for 4-6 hours in RPMI supplemented with human AB serum (Sigma-Aldrich) and CD107a antibody (clone H4A3 ...
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bioRxiv - Bioengineering 2022Quote: ... Filtered particles were either immediately introduced to cells (105 target cells plated in a 6-well plate) in antibiotic-free culture medium supplemented with 8 μg ml-1 polybrene (TR-1003-G, EMD Millipore) or stored in single use aliquots at −80ºC ...
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bioRxiv - Biochemistry 2022Quote: ... and a final SEC polishing step was performed on Superose® 6 10/300 Increase (Millipore Sigma, catalog # GE17-5172-01) pre-equilibrated in 10 mM HEPES pH 8.0 ...
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bioRxiv - Cell Biology 2022Quote: ... DNA was immobilized on a nitrocellulose membrane and then probed with antibodies against (6-4)PPs (Cosmo Bio NM-DND-002) or ssDNA (Millipore MAB3034). Chemiluminescence was detected as for protein immunoblotting ...
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bioRxiv - Cell Biology 2022Quote: ... Anti alpha tubulin-FITC mouse mAb (#F2168) and 4’,6-diamidine-20-phenylindole dihydrochloride (DAPI, #10236276001) were purchased from Sigma Aldrich, Germany ...
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bioRxiv - Cell Biology 2022Quote: With single hand restrain mice starved for 6 hrs were administered intra-peritoneally with 50mg/kg body weight of streptozotocin (Sigma Aldrich) for 6 consecutive days ...
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bioRxiv - Genomics 2022Quote: ... a single thymidine block was used to first arrest cells in S phase: cells were plated at 4×10^6 cells per 15 cm plate in medium containing 2mM Thymidine (Sigma, T1895), and were grown for 24 hours ...