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Citations for Millipore Sigma :
2551 - 2600 of 7599 citations for Zika Virus Envelope Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2022Quote: ... and associated proteins were eluted in 125 μg/ml 3xFLAG peptide (Sigma) for 30 minutes on ice.
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bioRxiv - Biophysics 2022Quote: ... and ASH2L proteins were individually expressed in Escherichia coli (Rosetta II, Novagen) and purified as described previously (66) ...
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bioRxiv - Biochemistry 2022Quote: ... His6-tagged proteins were purified with Ni-NTA His-Bind Superflow (Novagen) and dialyzed against PBS using a Pur-A-Lyzer™ Midi Dialysis Kit (Merck).
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bioRxiv - Cancer Biology 2022Quote: ... Protein G Sepharose 4 Fast Flow Beads (Millipore Sigma, GE17-0618-01) were added and incubated for 1 h at 4°C with rotation ...
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bioRxiv - Cell Biology 2022Quote: ... Proteins were resolved by SDS-PAGE and transferred to PVDF membrane (Millipore). Membranes were incubated overnight with primary antibodies (Antibody list shown in Table 3) ...
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bioRxiv - Microbiology 2020Quote: ... The proteins were then concentrated by ultrafiltration using Amicon filters (EMD Millipore).
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bioRxiv - Neuroscience 2019Quote: ... Flag-tagged proteins were immunoprecipitated using anti-Flag-M2 affinity gel (Sigma). Immunoprecipitates were incubated for at least 2 h at 4°C ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... 300 μg proteins were incubated with 20 mM dithiothreitol (DTT; Sigma Aldrich) for 10 min at 95°C on a thermomixer (Thermo Fisher Scientific) ...
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bioRxiv - Cell Biology 2019Quote: ... Proteins were transferred onto 0.45 µm PVDF Immobilon-FL membranes (Millipore Sigma) and visualized using the ChemiDoc XRS+ (Biorad ...
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bioRxiv - Neuroscience 2020Quote: ... Protein lysates were concentrated using Microcon-10kDa Centrifugal Filter Units (Merck Millipore), according to manufacturer’s instructions and protein concentration was determined using the Pierce BCA Protein Assay Kit (Thermo Fisher Scientific) ...
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African Swine Fever Virus CD2v protein promotes β-Interferon expression and apoptosis in swine cellsbioRxiv - Microbiology 2020Quote: ... and immunoprecipitated using 50 μl of protein G agarose bead slurry (Millipore). Monoclonal antibodies recognizing CD2v-HA were generated by immunizing BALB/c mice with immunoprecipitated CD2v-HA (100 μg/dose) ...
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bioRxiv - Genetics 2021Quote: ... Transfer was confirmed by visualising protein bands with 0.1% Ponceau S (Sigma) in 5% acetic acid (Sigma ...
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bioRxiv - Molecular Biology 2020Quote: ... The proteins were transferred to Immobilon-P Polyvinylidene fluoride (PVDF) membranes (Millipore) in Tris-Glycine-SDS buffer containing 20% methanol for 2h at 50V ...
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bioRxiv - Cancer Biology 2021Quote: ... proteins of interest were revealed by Clarity Western Blot ECL substrate (Millipore).
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bioRxiv - Neuroscience 2021Quote: ... and protein was eluted with 100 μg/mL FLAG peptide (Sigma-Aldrich) in elution buffer for 1 hour at 4°C with rotation ...
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bioRxiv - Molecular Biology 2020Quote: ... The chromatin solution was pre-cleared with protein A sepharose (Sigma-Aldrich), 10% retained (input ...
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bioRxiv - Immunology 2020Quote: ... Separated proteins were transferred to PVDF Immobilon-FL membranes (Millipore, Billerica, MA) using the BioRad Trans-Blot Turbo Transfer System (Hercules ...
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bioRxiv - Neuroscience 2021Quote: ... immunoreactive proteins were visualized using Immobilon® Classico Western HRP Substrate (Millipore) or Immobilon® Forte Western HRP Substrate (Millipore ...
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bioRxiv - Microbiology 2020Quote: ... Protein was reduced with tris (2-carboxyethyl) phosphine (Sigma-Aldrich, MO, USA) and alkylated with methylmethanethiosulphonate (MMTS ...
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bioRxiv - Physiology 2021Quote: ... All images were normalized to Amido Black total protein stain (A8181, Sigma) using the entire lane corresponding to each sample.
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bioRxiv - Biochemistry 2021Quote: ... Protein solution (4 mg/mL in 50 mM Tris-HCl (Sigma-Aldrich), pH 8.5 ...
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bioRxiv - Biochemistry 2021Quote: ... The fusion proteins were cleaved by incubation with thrombin protease (Sigma-Aldrich) and purified with a Superdex 200 pg (Cytiva ...
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bioRxiv - Neuroscience 2020Quote: ... the protein samples were reduced with 20 mmol/L dithiothreitol (DTT, Sigma) at 37 °C for 1 h and alkylated with 50 mmol/L iodoacetamide (IAA ...
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bioRxiv - Microbiology 2021Quote: ... or thrombin protease (10 U/mg of fusion protein, Sigma-Aldrich, Germany). Importin β1 ...
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bioRxiv - Microbiology 2021Quote: Immunoprecipitation was conducted using PureProteomeTM Protein A/G Mix Magnetic Beads (Millipore). Briefly ...
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bioRxiv - Neuroscience 2022Quote: ... Proteins were transferred to polyvinyl difluoride membranes (Immobilon-P, #IPVH00010, Millipore/Sigma) and incubated overnight at 4 °C with the primary anti-Plin2 antibody (ADFP rabbit polyclonal antibody ...
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bioRxiv - Neuroscience 2022Quote: ... Proteins were transferred to polyvinyl difluoride membranes (Immobilon-P, #IPVH00010, Millipore/Sigma) and incubated overnight at 4 °C with the primary anti-Plin2 antibody (ADFP rabbit polyclonal antibody ...
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bioRxiv - Developmental Biology 2022Quote: ... Subsequently protein thiol groups were blocked with 10mM iodoacetamide (Sigma-Aldrich, USA) at RT for 45 min ...
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bioRxiv - Genomics 2022Quote: Fusion protein overexpression was induced by treatment with doxycycline (dox) (Sigma Aldrich) at a concentration of 1µg/mL for the specified amount of time ...
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bioRxiv - Biochemistry 2022Quote: ... All reagents used for protein purification and enzymatic assays were from Sigma.
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bioRxiv - Neuroscience 2022Quote: ... protein content was additionally measured using the Bradford assay (Sigma-Aldrich, USA). Bovine serum albumin dissolved in 1xPBS was used for the standard curve ...
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bioRxiv - Immunology 2022Quote: The recombinant proteins were purified using His•Bind® Purification Kit (Novagen) following manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2022Quote: ... Proteins were probed with rabbit anti-HA (1:1,000 Sigma-Aldrich, H3663) or anti-β-actin as loading control (1:10,000 Sigma-Aldrich ...
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bioRxiv - Evolutionary Biology 2022Quote: ... Proteins were probed with mouse anti-FLAG (1:5,000 Sigma-Aldrich, F3165) or anti-β-actin (1:5,000 Sigma-Aldrich ...
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bioRxiv - Immunology 2022Quote: ... mice received either 5 nmol Endo Grade® Ovalbumin protein (Sigma-Aldrich) alone ...
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bioRxiv - Immunology 2022Quote: ... proteins were transferred to a methanol-activated PVDF-FL membrane (Millipore Sigma) in NuPAGE transfer buffer (Invitrogen ...
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bioRxiv - Bioengineering 2022Quote: ... and fibrinogen from bovine plasma (Type I-S, ≥75% clottable protein, Sigma). A 6-8 wt% gelatin stock solution was made by dissolving lyophilized gelatin in calcium and magnesium-free phosphate-buffered saline (10 mM PBS ...
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bioRxiv - Neuroscience 2022Quote: ... Immunohistochemistry was performed using glial fibrillary acidic protein (GFAP) (1:1000; Millipore) and ionized Ca2+-binding adapter protein (1:1000 ...
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bioRxiv - Cancer Biology 2022Quote: ... Proteins were transferred onto methanol-activated Immobilon-P PVDF membranes (Merk Millipore) using a Mini-Transblot Cell (Bio-Rad) ...
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bioRxiv - Biochemistry 2019Quote: ... Selenomethionine substituted proteins were overexpressed in Escherichia coli B834 (DE3) cells (Novagen) using PASM-5052 autoinduction media (Studier 2005) ...
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bioRxiv - Immunology 2019Quote: ... Rabbit anti-Myc Abs and Protein A-HRP were purchased from Sigma and Southern Biotech ...
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bioRxiv - Biochemistry 2019Quote: ... The protein samples were reduced with 20 mmol/L dithiothreitol (DTT, Sigma) at 37 °C for 1 h and then added to 50 mmol/L iodoacetamide (IAA ...
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bioRxiv - Neuroscience 2019Quote: ... The separated proteins were transferred onto Immobilin-FL PVDF membrane (EMD Millipore), blocked for 1 hour with Odyssey blocking buffer (LICOR ...
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bioRxiv - Cell Biology 2019Quote: Proteins were separated by SDS-PAGE and transferred to nitrocellulose membranes (Millipore). Membranes were blocked with LiCor blocking buffer and incubated with the indicated antibodies ...
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bioRxiv - Molecular Biology 2019Quote: ... supernatant was incubated with protein A+G magnetic beads (Millipore 16-663) for 1h for preclearing at 4°C ...
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bioRxiv - Cell Biology 2020Quote: ... The recombinant protein was expressed in Rossetta2(DE3) strain (EMD Millipore/Novagen). Expressed protein was found in inclusion body thus the proteins were solubilized by 8M urea containing buffer (20mM Hepes pH7.8 ...
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bioRxiv - Cell Biology 2020Quote: ... The recombinant protein was expressed in Rossetta2(DE3) strain (EMD Millipore/Novagen). Expressed protein was found in inclusion body thus the proteins were solubilized by 8M urea containing buffer (20mM Hepes pH7.8 ...
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bioRxiv - Developmental Biology 2019Quote: ... Then the proteins were transferred onto a polyvinylidene fluoride (PVDF) membrane (Millipore) using a Mini Trans Blot system (Bio-Rad) ...
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bioRxiv - Cancer Biology 2019Quote: ... Protein content of lysed samples was quantified using BCA assay (Sigma-Aldrich). MILLIPLEX MAP Akt/mTOR phosphoprotein kit ...
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bioRxiv - Cell Biology 2019Quote: ... Proteins were probed with rabbit anti-human Cx43 (1:2000) (Sigma-Aldrich) and rabbit anti-ß-Tubulin antibodies (1:2000 ...