Labshake search
Citations for Millipore Sigma :
2451 - 2500 of 7599 citations for Zika Virus Envelope Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
Trypanosoma brucei J protein 2 functionally cooperates with the cytosolic Hsp70.4 and Hsp70 proteinsbioRxiv - Molecular Biology 2019Quote: ... The protein yield was estimated using the Bradford assay (Sigma-Aldrich, USA) with BSA as the standard ...
-
bioRxiv - Biophysics 2020Quote: ... polyclonal antibody against brain lipid binding protein (BLBP) (Millipore, Merck KGaA, Germany), mouse antibody against nestin (Millipore ...
-
bioRxiv - Developmental Biology 2019Quote: Protein was extracted using 1X RIPA lysis buffer (Millipore Sigma, Burlington MA) supplemented with Halt protease inhibitor cocktail (Thermo Fisher Scientific) ...
-
bioRxiv - Cancer Biology 2019Quote: ... immunoreactive proteins were detected by an enhanced chemiluminescence detection system (EMD-Millipore).
-
bioRxiv - Pharmacology and Toxicology 2020Quote: ... The separated proteins were then transferred to PVDF membranes (0.45μm, Millipore, USA), and block with 5% milk for 1h ...
-
bioRxiv - Immunology 2019Quote: ... The protein bands were visualized by Immobilon Western Chemiluminescent HRP Substrate (Millipore) according to the manufacturer’s instructions.
-
bioRxiv - Microbiology 2019Quote: ... Protein concentration was determined using a microplate Bradford (Sigma –Aldrich Bradford Reagent) protein assay with bovine serum albumin standards.
-
bioRxiv - Cell Biology 2020Quote: ... we use the PureProteome Protein G Magnetic Bead System (EMD Millipore Corp.). 1 mg protein extract was used for immunoprecipitation and incubated with protein G beads and 4 μg mouse anti-GFP antibody (B-2 #sc-9996 ...
-
bioRxiv - Neuroscience 2019Quote: ... Protein concentration was measured using the bicinchoninic acid assay (BCA) kit (Sigma).
-
bioRxiv - Biochemistry 2019Quote: ... the reconstituted protein was mixed with 50 μM ADP-Ribose (Sigma-Aldrich) and further purified on a Superose 6 10/300 GL column (GE Healthcare ...
-
bioRxiv - Cell Biology 2019Quote: ... proteins were visualized by staining with Amido Black staining solution (Sigma-Aldrich) according to manufacturer’s directions.
-
bioRxiv - Genetics 2019Quote: ... and then captured by protein G-agarose/salmon sperm DNA (Millipore Sigma). Precipitated DNA was reverse cross-linked and then amplified by qPCR using primers amplifying amplicons with the following midpoints relative to the transcription start site of Shox2 ...
-
bioRxiv - Microbiology 2019Quote: ... and tagged proteins were detected with mouse anti-GFP (Sigma, 1:5,000), rat anti-HA (Sigma 3F10 clone ...
-
bioRxiv - Cell Biology 2019Quote: ... Separated proteins were then transferred to a PVDF membrane (IPVH00010, Millipore Corp.) for overnight at 4°C in a wet transfer system (Hoefer ...
-
bioRxiv - Biochemistry 2019Quote: ... whole cell lysates (WCLs) were precleared using protein A Sepharose (Sigma-Aldrich) for 30 minutes at 4°C ...
-
bioRxiv - Immunology 2019Quote: ... Proteins were resolved by SDS-PAGE and transferred to PDVF membranes (Millipore) before immunoblotting with anti-HA (12CA5 ...
-
bioRxiv - Pharmacology and Toxicology 2020Quote: ... the protein samples were treated with 10 mM dithiothreotol (DTT) (Sigma, MO) at 56 °C for 1 h and 55 mM IAA (Sigma ...
-
bioRxiv - Plant Biology 2019Quote: ... 0.2% Igepal CA-630 and Protein Inhibitor Cocktail (Sigma-Aldrich; http://www.sigmaaldrich.com/). The cell suspension was frozen in liquid nitrogen and then thawed on ice and centrifuged for 5 min at 150 g ...
-
bioRxiv - Animal Behavior and Cognition 2019Quote: ... Electrophoresis proteins were transferred to PVDF membranes (PVDF membrane, 0.45μm; MILLIPORE, USA) using a Bio-Red Laboratories Instruments Mini Trans-Blot Electrophoretic Transfer Cell unit (Alfred Nobel Drive Hercules ...
-
bioRxiv - Microbiology 2020Quote: ... and proteins were purified over HIS-Select (Sigma–Aldrich, St. Louis, MO) nickel affinity resin in 20 mM Tris (pH 8.0 ...
-
bioRxiv - Physiology 2020Quote: ... proteins were transferred to a PVDF membrane (Immobilon-P, Merck Millipore, IPVH00010) with 160 mA for 90 min ...
-
bioRxiv - Biochemistry 2019Quote: ... Proteins were affinity purified by anti-Flag M2 affinity resin (Sigma-Aldrich), which followed digestion of affinity tag and deglycosilation by TEV protease and MBP-fusion endoglycosidase (New England Biolabs) ...
-
bioRxiv - Plant Biology 2019Quote: ... and the separated proteins were transferred to PVDF membrane (Millipore, Billerica, MA), stained with Ponceau S for labeling of total protein ...
-
bioRxiv - Molecular Biology 2019Quote: ... protein expression was induced by using 1 μg/ml doxcycline (Sigma Aldrich) for 24h.
-
bioRxiv - Plant Biology 2020Quote: ... and proteins were detected after blotting with anti-FLAG M2 antibody (SIGMA) (1:10,000 dilution ...
-
bioRxiv - Cell Biology 2019Quote: ... Proteins were visualized by ECL (Enhanced chemiluminescence; Merck Millipore, Cat. No. WBKLS0500). The signal was detected using Vilber FUSION-SL system ...
-
bioRxiv - Cell Biology 2019Quote: ... protein expression was induced by using 1 μg/ml doxcycline (Sigma Aldrich) for 24h and checked by Western blot analysis.
-
bioRxiv - Genetics 2020Quote: ... Nuclear and cytoplasmic protein extracts were prepared using NXTRACT kit (Sigma-Aldrich) according manufacturer’s instructions.
-
bioRxiv - Genetics 2021Quote: ... clarification and benzonase treatment (250 u/1 mg protein, SIGMA E1014-5KU) extracts were incubated 1h at 4°C with 50 µL magnetic beads (Dynabeads M-280 sheep anti-mouse IgG ,invitrogen 11202D ...
-
bioRxiv - Genetics 2020Quote: ... Protein extraction was carried out on ice using RIPA buffer (Sigma Aldrich) supplemented with protease inhibitor cocktail (Sigma) ...
-
bioRxiv - Genomics 2021Quote: ... complexed with 6 mg pre-swollen protein A Sepharose beads (Sigma-Aldrich) during a 2-h incubation at 4°C ...
-
bioRxiv - Genetics 2020Quote: ... and proteins were eluted using 100 µg/mL 3x FLAG peptide (Sigma). Eluates were analysed by western blotting with anti-FLAG antibodies (1:5000 ...
-
bioRxiv - Cell Biology 2021Quote: ... g) were pre-cleared with protein A-immobilized PureProteome magnetic beads (Millipore) and incubated overnight (4°C ...
-
Molecular basis of selective cytokine signaling inhibition by antibodies targeting a shared receptorbioRxiv - Immunology 2021Quote: ... 2000 response units (RU) of Protein A from Staphylococcus aureus (Sigma Aldrich) were immobilized on all channels of a CM5 sensor chip ...
-
Molecular basis of selective cytokine signaling inhibition by antibodies targeting a shared receptorbioRxiv - Immunology 2021Quote: ... 2000 response units (RU) of Protein A from Staphylococcus aureus (Sigma Aldrich) were immobilized on all channels of a CM5 sensor chip ...
-
bioRxiv - Neuroscience 2020Quote: ... before the protein expression was revealed by chemiluminescence using Immobilon Western (Millipore) and the signal detected using a Chemidoc MP system (BioRad) ...
-
bioRxiv - Biochemistry 2021Quote: ... Bound protein was detected using anti-His-HRP conjugate (Sigma; 1:10,000). All proteins and antibodies were in 0.1 M phosphate buffer ...
-
bioRxiv - Biochemistry 2021Quote: ... All remaining protein constructs were cloned into the pET28b vector (Merck Millipore). Unless otherwise stated ...
-
bioRxiv - Cancer Biology 2021Quote: Proteins were extracted from cell culture by RIPA lysis buffer (Sigma Aldrich) with protease and phosphatase inhibitor cocktail and sample reducing agent (Thermo Fisher Scientific) ...
-
bioRxiv - Biochemistry 2020Quote: ... Protein-antibody binding was visualized using Immobilon Forte Western HRP Substrate (Millipore) on the LAS-3000 imaging system (FUJIFILM ...
-
bioRxiv - Bioengineering 2021Quote: ... filtered through a Millex-HV 0.45-μm low protein-binding membrane (Millipore), and aliquoted ...
-
bioRxiv - Bioengineering 2021Quote: ... Supernatant containing soluble recombinant protein was loaded into α-lactose agarose (Sigma) affinity column preequilibrated with PBS ...
-
bioRxiv - Bioengineering 2021Quote: ... Proteins were eluted with a linear gradient of β-lactose (Sigma-Aldrich) in phosphate buffer.
-
bioRxiv - Neuroscience 2021Quote: ... Protein was extracted from transfected meningeal cells using RIPA buffer (R0278, Sigma) and run on Western blot at 20ug per well ...
-
bioRxiv - Bioengineering 2020Quote: ... total protein was collected from cell lysate in RIPA buffer (Sigma, R0278) containing 1% phosphatase inhibitor cocktail 3 (Sigma ...
-
bioRxiv - Biochemistry 2021Quote: ... Proteins were visualized by using enhanced chemiluminescence (Millipore Corporation, Billerica, MA, USA). Images were acquired using Uvitec Image System (Cleaver Scientific Limited ...
-
bioRxiv - Biochemistry 2020Quote: ... The partially purified protein was concentrated (10 kDa cut-off, EMD Millipore) and exchanged into 100 mM potassium phosphate buffer (pH 8.0 ...
-
bioRxiv - Biochemistry 2020Quote: ... The protein was loaded onto a 0.45 μm nitrocellulose filter (HA Millipore), and the filters were washed with 3 ml 30 mM imidazole (pH 7.1) ...
-
bioRxiv - Biophysics 2021Quote: Routine chemicals used for protein expression and purifications were purchased from Sigma, Fisher Scientific ...
-
bioRxiv - Neuroscience 2021Quote: ... Supernatant was recovered and proteins were quantified with Bradford assay (Sigma Aldrich) and samples were stored at -80°C until further used ...