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Citations for Millipore Sigma :
2551 - 2600 of 7138 citations for Cleaved PARP Rabbit Recombinant mAb since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2023Quote: We performed the phosphorylation kinetics presented in Figure 4a using commercial recombinant kinases GST-p38βat 10 μg/mL (Sigma-Aldrich, ref. B4437), GST-Erk2 at 20 μg/mL (Sigma-Aldrich ...
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bioRxiv - Microbiology 2023Quote: BCBL-1/Cas9 cells containing pLenti SpBsmBI Guide Hygro (AAVS1 or TRAIL-R1 #1/2) were treated with the indicated serial dilutions of recombinant human TRAIL (Sigma-Aldrich, T9701) and absolute cell numbers were measured 24 hours later by flow cytometry as described above for cumulative growth curves.
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bioRxiv - Molecular Biology 2023Quote: ... The PCR products from each targeted exon were purified and mixed with 0.5 units of recombinant spCas9-GFP (Sigma-Aldrich, Cat # CAS9GPRO), 10 μM gRNA in 2 μL of 10x Cas9 reaction buffer ...
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bioRxiv - Microbiology 2023Quote: ... were transformed with the lithium acetate method 45 and strains containing the recombinant plasmids were selected on YPD agar with 200 μg/mL geneticin (G418 – Sigma A1720-5G).
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bioRxiv - Cell Biology 2023Quote: The custom recombinant antibody-like anti-MAR binding reagent (anti-MAR) was generated and purified in-house (now available from Millipore Sigma, MABE1076) (Gibson et al. ...
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bioRxiv - Cell Biology 2024Quote: ... The recombinant protein from the cell lysate was purified using Glutathione Sepharose® 4B GST affinity columns (Millpore Sigma, GE17-0756-05). The purified PCM1-GST fusion protein was analyzed on SDS-PAGE stained with Coomassie Blue (Invitrogen) ...
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bioRxiv - Immunology 2024Quote: ... the nitrocellulose membranes containing the purified recombinant fusion proteins were incubated with mouse anti-His antibody [clone HIS.H8] (Millipore Sigma, Burlington, Massachusetts). HRP-conjugated rabbit anti-mouse IgG antibody (SouthernBiotech ...
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bioRxiv - Cell Biology 2022Quote: ... rabbit polyclonal antibody AB37086 (1:2500) and actin using a rabbit polyclonal antibody from Sigma-Aldrich (#A2066; 1:8000). HRP-tagged secondary antibodies (Bio-Rad ...
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bioRxiv - Cell Biology 2021Quote: ... and incubation overnight at 4°C with primary antibodies (rabbit anti-H3K9me3, Abcam, ab8898, 1:1,000; rabbit anti-H3K27me3, Millipore, 07-449 ...
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bioRxiv - Cell Biology 2021Quote: ... and then incubated overnight at 4°C with primary antibodies (rabbit anti-H3K9me3, Abcam, ab8898, 1:3,000; rabbit anti-H3K27me3, Millipore, 07-449 ...
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bioRxiv - Microbiology 2020Quote: ... horseradish peroxidase-conjugated rabbit anti-rat IgG and horseradish peroxidase-conjugated goat anti-rabbit IgG (Sigma, St. Louis, MO) were used for Western blotting at dilutions of 1:10,000 ...
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bioRxiv - Biochemistry 2021Quote: ... Primary rabbit polyclonal anti-(human Nav1.5), (residues: 1978-2016) (#ASC-013, Alomone Labs) and rabbit polyclonal anti-(actin) (A2066, Sigma), both used at a concentration of 1:200 were followed by a one-hour incubation with secondary Goat anti-(Rabbit IgG-HRP conjugate ...
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bioRxiv - Cancer Biology 2024Quote: ... samples stained with primary FBXO11 (mouse) and LONP1 (rabbit) antibodies were incubated with Anti-Rabbit PLUS (Sigma-Aldrich DUO92002) and Anti-Mouse MINUS (Sigma-Aldrich DUO92004 ...
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bioRxiv - Cell Biology 2023Quote: ... Rabbit or mouse primary antibodies were detected using a horseradish peroxidase-conjugated anti-rabbit IgG (Sigma AP307P, 1:4000) or anti-mouse IgG (Sigma AP308P ...
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bioRxiv - Developmental Biology 2023Quote: Membranes were incubated overnight in primary antibody o/n at 4°C: Rabbit ⍺-Vang (Rawls & Wolff, 2003) diluted 1:1,000 in 5% milk in TBST or Rabbit ⍺-Actin (Sigma) diluted 1:2,000 in 10% Seablock in TBST followed by 3x wash in TBST for 10 minutes each ...
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bioRxiv - Cell Biology 2023Quote: ... Rabbit anti-phospho-ERM (1/5000) (Roubinet et al., 2011) and rabbit anti-actin (Sigma-Aldrich A5060, 1/10,000) were also used ...
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bioRxiv - Cell Biology 2024Quote: ... Rabbit polyclonal anti-PEX14 was obtained from ProteinTech (cat. 10594-1-AP). Rabbit anti-calnexin (cat. C4731) and rabbit anti-TGN46 (cat. T7576) were from Sigma-Aldrich. Mouse anti-p230 was from BD Bioscience (cat ...
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bioRxiv - Molecular Biology 2019Quote: The recombinant IFNε protein was purified using a single-step High-Select High Flow (HF) nickel affinity chromatographic gel (Sigma-Aldrich, Cat. # H0537). The nickel affinity column (1.0 cm × 1.0 cm ...
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bioRxiv - Microbiology 2020Quote: ... were immunized intraperitoneally with 17 μg of recombinant NRP1 b1b2 mixed with an equal volume of complete Freund’s adjuvant (Sigma–Aldrich Chemie, Steinheim, Germany), followed by a booster immunization four weeks later of the same dose mixed with incomplete Freund’s adjuvant (Sigma–Aldrich) ...
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bioRxiv - Biochemistry 2020Quote: ... The production of the (His)6-tagged recombinant proteins was confirmed by Western analysis using mouse monoclonal α-His-horseradish peroxidase conjugated antibodies (Sigma Aldrich, USA). Imaging of the protein bands on the Western blot was conducted using the ECL kit (ThermoScientific ...
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bioRxiv - Neuroscience 2020Quote: 100 μg of recombinant Tau 2N4R were incubated for 2h at room temperature in 100 mM sodium citrate pH 6 (Sigma, cat. no. W302600), 100 mM sodium cyanoborohydride (Sigma ...
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bioRxiv - Biophysics 2021Quote: ... The recombinant expression constructs were transfected into CHO Tet-On cells and expression was carried out in the presence of biotin as previously described.18 Culture medium containing secreted recombinant A1 or A1A2A3 protein was collected and concentrated five times using an Amicon Ultra-15 Centrifugal Filters (EMD Millipore, Billerica, MA). Free biotin was removed from the samples using a desalting PD-10 column (GE Healthcare ...
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bioRxiv - Genomics 2022Quote: ... in a 12-well cell culture plate coated with Human recombinant laminin 511 (BioLamina, Cat. LN511-0202) and fixed in 1xPBS with 3.7% formaldehyde (Millipore Sigma, Cat. F8775-25ML) for 10 mins and then permeabilized in ice-cold 70% (vol./vol. ...
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bioRxiv - Plant Biology 2022Quote: ... approximately 100 μg His-IAA17 recombinant proteins were treated with 200 μM GSNO and then labeled with biotin-maleimide (Sigma-Aldrich, Cat #: B1267). After biotinylated proteins were precipitated with cold acetone ...
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bioRxiv - Neuroscience 2022Quote: Organotypic entorhino-hippocampal tissue cultures (≥ 18 days in vitro) were treated with mouse recombinant Tumor Necrosis Factor α (60 pM or 6nM; Sigma, Cat# T7539) for 24h ...
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bioRxiv - Bioengineering 2023Quote: ... ethylenediaminetetraacetic acid (EDTA), iodoacetamide (IAM), urea, recombinant porcine trypsin, K2IrCl6, and phosphate buffered saline (PBS, pH 7.4) were purchased from Sigma-Aldrich (St. Louis, MO). L-histidine monohydrochloride monohydrate and L-histidine were purchased from Avantor (Allentown ...
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bioRxiv - Biochemistry 2024Quote: ... pCD44 was generated by pretreatment of recombinant murine CD44-Fc-AviTag with CK2α in PBS with 10 mM MgCl2 and 1 mM ATP (Sigma Aldrich, A1852-1VL). DNA was harvested from the libraries before and after sorting using Zymoprep Yeast Plasmid Miniprep II (Zymo Research ...
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bioRxiv - Microbiology 2023Quote: ... Cells that received Interferon-β pre-treatment were stimulated for 18 hours with 100 units of recombinant human Interferon-β protein (Millipore, IF014) prior to infection.
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bioRxiv - Cell Biology 2023Quote: ... East Brunswick, NJ, USA), human recombinant Stem Cell Factor (100ng/mL, supernatant SCF producing cell line) and dexamethasone (1μM; Sigma, St. Louis, MO, USA).5 EBL cultures were kept at a density of 0.7-1.5 million/mL by dilution with fresh medium ...
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bioRxiv - Microbiology 2024Quote: ... for NF10 production or with 50 μg of each of five recombinant LMWs in alum mixed with 200 ng/mouse pertussis toxin (Sigma-Aldrich, MO, USA) for QD8 production ...
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bioRxiv - Cell Biology 2019Quote: ... and incubated overnight at 4°C with primary antibodies (rabbit anti-TMEM98, Proteintech, 1:1000; rabbit anti-FLAG polyclonal, Sigma, 1:2000 ...
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bioRxiv - Plant Biology 2021Quote: ... PVDF membranes were blocked with 5% milk followed by an incubation with first antibodies for REM1.2/1.3 followed by 3 washes and an incubation with second α-rabbit antibody fused to HRP against rabbit (Sigma). Clarity Western 701 ECL (Bio-Rad ...
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bioRxiv - Molecular Biology 2020Quote: ... The membrane was washed twice more before incubating with the secondary antibody (Anti-goat IgG raised in rabbits for the Mu and Anti-rabbit IgG raised in goats for the Sigma) for 1-2 hours at a 1:30000 dilution in blocking buffer ...
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bioRxiv - Cell Biology 2020Quote: ... Proteins were immunodetected using 1:2500 dilution of the rabbit anti-TbKH polyclonal antibody and 1:15,000 dilution of goat anti-rabbit-HRP antibody (Sigma-Aldrich). The chemiluminescent protocol was used for developing as indicated below.
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bioRxiv - Cell Biology 2022Quote: ... 2013), rabbit anti-Cep97 (Dobbelaere et al., 2020), rabbit anti-Cnn (Dobbelaere et al., 2008) and mouse anti-Actin (Sigma); all primary antibodies were used at 1:500 dilution in blocking solution (Aydogan et al. ...
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bioRxiv - Cell Biology 2022Quote: ... overnight and immunostained using primary rabbit anti-Cox4 (1:1000) or anti-Por1 (dilution 1:5000) for mitochondrial porin and secondary anti-rabbit (Sigma) antibodies ...
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bioRxiv - Microbiology 2019Quote: ... mouse anti-rabbit IgG antibody:alkaline phosphatase (Sigma-Aldrich; anti-mouse IgG (whole molecule:alkaline phosphatase antibody produced in rabbit (Sigma-Aldrich), and anti-rabbit IgG ...
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bioRxiv - Immunology 2020Quote: ... C1q fixation was detected with rabbit anti-C1q antibodies (1/2000; in-house) and a goat anti-rabbit-HRP (1/2500; Millipore). TMB liquid substrate (Life Technologies ...
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bioRxiv - Genetics 2019Quote: ... The infectious blood meal was composed of a virus suspension diluted (1:3) in washed rabbit blood and resuspended at 50% (vol/vol) in dialyzed rabbit serum (R4505; Sigma). ATP was added to a final concentration of 5 μM ...
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bioRxiv - Microbiology 2022Quote: ... SY0917 (MA11010) SABC-Serum) (rabbit, 1:10,000) and a secondary anti-rabbit-HRP (goat) conjugate antibody (1:10,000, Sigma-Aldrich, #31460). The rest of the samples was prepared for mass spectrometric analysis as previously described (Hör et al. ...
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bioRxiv - Plant Biology 2024Quote: ... and subjected to immunoblot analysis with the relevant primary and secondary antibodies (anti HA rabbit polyclonal, Abcam ab20084; anti AV2 CP rabbit polyclonal, DSMZ RT-0953-0645/1; anti c-Myc mouse monoclonal, Sigma M4439 ...
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bioRxiv - Cell Biology 2023Quote: ... The pre-cleared chromatin was then incubated overnight at 4°C with 10 µL of either rabbit IgG (1 mg/mL, IGBMC facility) or rabbit anti-CTCF (07-729, Millipore). Immunoprecipitated chromatin was then adsorbed to 100 µL prepared protein A/protein G-Dynabeads for 3.5 h at 4°C on a rotating wheel ...
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bioRxiv - Cancer Biology 2023Quote: ... rabbit anti-CYR61 (Bio-Techne SAS #NB100-356; diluted 1:1000) or rabbit anti-beta Actin (Sigma #; diluted 1:2000). After three washes with TBST buffer [50 mM Tris/HCl pH 7.4 ...
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bioRxiv - Cancer Biology 2024Quote: ... anti-FLAG-tag (CST, rabbit, 1:1000), anti-total-ERK1/2 (CST, rabbit, 1:1000) diluted in PBS containing 1% BSA (Sigma); and secondary antibody anti-rabbit (CST ...
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bioRxiv - Immunology 2024Quote: ... C1q fixation was detected with rabbit anti-C1q antibodies (1/2000; in-house) and a goat anti-rabbit-HRP (1/2500; Millipore). For FcγR assays ...
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bioRxiv - Systems Biology 2024Quote: ... The cells were then incubated with 1ug/ul primary antibody (For TRUB1 staining, TRUB1 Rabbit anti-human polyclonal, 50-172-8037, Protein tech; for PUS7 staining, PUS7 Rabbit anti-human, HPA024116, Sigma) in 1% BSA/PBS-Triton (0.1% ...
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bioRxiv - Microbiology 2020Quote: ... Antibodies used were rabbit anti-ETV7 (Sigma, HPA029033), rabbit anti-IFIT1 (Cell Signaling ...
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bioRxiv - Biophysics 2021Quote: ... or/and rabbit anti-Tom20 antibody (HPA011562, Sigma). The secondary antibodies were DL633 labeled Goat anti-Mouse IgG (A-21235 ...
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bioRxiv - Cancer Biology 2021Quote: ... rabbit anti-CCSP (1:5000, Millipore, 07-623), rabbit anti-Keratin 5 (1:5000 ...
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bioRxiv - Neuroscience 2020Quote: ... rabbit anti-GAT3 (1:250, Millipore/Chemicon, AB1574); rabbi anti-NeuN (1:500 ...