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Citations for Millipore Sigma :
2401 - 2450 of 6791 citations for Cleaved PARP Rabbit Recombinant mAb since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... TDP-43 (rabbit polyclonal C-terminal, Sigma); FUS (mouse monoclonal ...
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bioRxiv - Cell Biology 2024Quote: ... TDP-43 (rabbit polyclonal, C-terminal, Sigma); eIF2α (rabbit polyclonal ...
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bioRxiv - Neuroscience 2023Quote: ... Rabbit Anti-TBR2 (ab15894 Millipore,1:50). AlexaFluor secondary antibodies (Thermo Fisher Scientific ...
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bioRxiv - Bioengineering 2024Quote: ... Rabbit anti-Cx43 (1:400, Sigma, C6219) was used as a primary antibody with a goat anti-rabbit secondary antibody conjugated to either Alexa Fluor 488 or 633 (1:500 ...
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bioRxiv - Cancer Biology 2024Quote: ... rabbit anti-substance P (Sigma-Aldrich, AB1566), mouse anti-neurofilament-L (Cell Signaling ...
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bioRxiv - Cancer Biology 2024Quote: ... anti-rabbit Rrp1b (1 μg) (Millipore-Sigma), anti-rabbit HA (5 μg ...
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bioRxiv - Cancer Biology 2024Quote: ... anti-rabbit H4K20ac (5 μg) (Millipore-Sigma), and anti-rabbit p300 antibody (0.57 μg ...
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bioRxiv - Cancer Biology 2024Quote: ... anti-rabbit Rrp1b (1 μg) (Millipore-Sigma), anti-rabbit HA (5 μg ...
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bioRxiv - Cancer Biology 2024Quote: ... anti-rabbit H4K20ac (5 μg) (Millipore-Sigma), and anti-rabbit p300 antibody (0.57 μg ...
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bioRxiv - Cancer Biology 2024Quote: ... rabbit anti-H3Ac (Millipore Sigma, 06-599), mouse anti-Annexin V (Santa Cruz ...
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bioRxiv - Cell Biology 2024Quote: ... and anti-Rabbit IgG (A9196, Sigma-Aldrich) at 1:2,000 and 1:10,000 dilutions ...
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bioRxiv - Cell Biology 2024Quote: ... and rabbit anti-MCM2 (SP85) from Sigma; pSAMHD1 ProSci (Poway ...
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bioRxiv - Cell Biology 2024Quote: ... and rabbit-anti-GAPDH (1:5,000, Sigma). Secondary antibodies fused to HRP and ECL (ThermoScientific ...
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bioRxiv - Biochemistry 2023Quote: ... rabbit α-OGT (1:2,000 ; Sigma Aldrich).
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bioRxiv - Cell Biology 2024Quote: ... rabbit anti-pS1141WRN (Sigma-Aldrich, 1:1000); rabbit anti-pS1133WRN (Genscript-custom ...
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bioRxiv - Cell Biology 2021Quote: ... P3 2M (AMS biotechnology #GSC-6201G 2M or #GSC-6101G 7M) and LIF ESGRO® Recombinant Mouse LIF Protein (1000 units/mL) (Millipore # ESG1107). For induction of the transgene Stemolecule Doxycycline hyclate 10 mg (Stemgent#04-0016 ...
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bioRxiv - Developmental Biology 2020Quote: ... individual components can be added to 500 ml media bottle at the indicated final concentrations: 1) Recombinant Human Insulin (Sigma I-1882) – 12.5 µg/ml final concentration ...
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bioRxiv - Molecular Biology 2021Quote: ... The pelleted cells were lysed and used to purify the recombinant proteins with glutathione–Sepharose 4B beads (Sigma Aldrich #GE17-0756-01). The purified protein or whole cell lysate was incubated with the respective agarose beads at various time points with continuous agitation and washed before imaging ...
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bioRxiv - Cell Biology 2022Quote: ... were used as a substrate for phospho-PKCα in a 25 µl in vitro kinase reaction using 100 ng of active recombinant PKCα enzyme (Millipore-Sigma, MA), 5 µl of a lipid activator (Millipore-Sigma ...
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bioRxiv - Biophysics 2020Quote: Both unlabeled and 15N labeled recombinant full-length αS was expressed and purified in Rosetta™ 2(DE3) pLysS (EMD Millipore®) cells using IMPACT™ system protocol (New England Biolabs® ...
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bioRxiv - Evolutionary Biology 2022Quote: The enzyme assay for the zebrafish Irg1-like was performed by incubating 7 µg recombinant protein with 1 mM cis-aconitate (Sigma A3412-1G) in 300 µl reaction buffer (10 mM HEPES ...
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bioRxiv - Cell Biology 2021Quote: ... The organoids were then allowed to grow for 5 days followed by 24 hour treatment with recombinant human protein TNF-α (H8916, Sigma Aldrich) at 40ng/ml or IFN-gamma (PHC4031 ...
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bioRxiv - Microbiology 2020Quote: ... 0.1 ng/ml human recombinant epithelial growth factor and 50 U/ml penicillin and 50 U/ml streptomycin (Sigma-Aldrich, MO, USA). The VK2 cells were seeded into 24-well tissue culture plates ...
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bioRxiv - Microbiology 2021Quote: ... were immunized intraperitoneally with 100 µl of a 1:1 emulsion containing 50 µg recombinant protein LukS or LukF and incomplete Freund’s adjuvant (Sigma-Aldrich, Taufkirchen, Germany). Mice were boosted with an emulsion of protein and incomplete Freund’s adjuvant at day 14 and 28 and bled after six weeks ...
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bioRxiv - Microbiology 2021Quote: N-terminal His-tagged recombinant proteins were expressed in Rosetta™ 2(DE3) pLysS competent cells (71403; Novagen, Inc., Madison, WI, USA) by induction with 0.5 mM isopropyl β-d-1-thiogalactopyranoside at 30°C for 4 h (N ...
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bioRxiv - Developmental Biology 2022Quote: ... containing the same supplements) freshly supplemented with 25 ng/mL of human recombinant FGF4 (prepared in house) and 1 μg/mL heparin (Sigma-Aldrich, USA). TSCs were passaged every 3-5 days ...
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bioRxiv - Cell Biology 2022Quote: Recombinant His6-EGFP used in the GFP intensity calibration (Figure 1B) was expressed in Rosetta 2 (DE3) competent cells (Millipore Sigma #71400) using pDual-EGFP plasmid (Addgene ...
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bioRxiv - Biochemistry 2023Quote: We performed the phosphorylation kinetics presented in Figure 4a using commercial recombinant kinases GST-p38βat 10 μg/mL (Sigma-Aldrich, ref. B4437), GST-Erk2 at 20 μg/mL (Sigma-Aldrich ...
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bioRxiv - Microbiology 2023Quote: BCBL-1/Cas9 cells containing pLenti SpBsmBI Guide Hygro (AAVS1 or TRAIL-R1 #1/2) were treated with the indicated serial dilutions of recombinant human TRAIL (Sigma-Aldrich, T9701) and absolute cell numbers were measured 24 hours later by flow cytometry as described above for cumulative growth curves.
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bioRxiv - Molecular Biology 2023Quote: ... The PCR products from each targeted exon were purified and mixed with 0.5 units of recombinant spCas9-GFP (Sigma-Aldrich, Cat # CAS9GPRO), 10 μM gRNA in 2 μL of 10x Cas9 reaction buffer ...
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bioRxiv - Microbiology 2023Quote: ... were transformed with the lithium acetate method 45 and strains containing the recombinant plasmids were selected on YPD agar with 200 μg/mL geneticin (G418 – Sigma A1720-5G).
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bioRxiv - Cell Biology 2023Quote: The custom recombinant antibody-like anti-MAR binding reagent (anti-MAR) was generated and purified in-house (now available from Millipore Sigma, MABE1076) (Gibson et al. ...
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bioRxiv - Developmental Biology 2024Quote: ... Serial dilutions from 2μg to 0.0625μg of recombinant SPARC (SRP3159, Sigma-Aldrich; S5174-25UG, Sigma-Aldrich; 942-SP-050, R&D Systems) were spotted and used as standards ...
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bioRxiv - Immunology 2024Quote: ... The coating of the Ibidi® µ-slides IV 0,4 Uncoated (Cat# 80601) with fibrin was performed by adding PBS mixed with recombinant human albumin (Sigma-Aldrich, Cat# A9731), fibrinogen from human plasma AF488 Conjugate (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2024Quote: TKO and QKO mESC were cultured in Serum/LIF media (DMEM-KO, 15% FBS, 1000 U/ml murine recombinant LIF (Millipore Sigma #ESG1106), 1× nonessential amino acids (100x ...
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bioRxiv - Molecular Biology 2024Quote: ... insulin-like growth factor 1 (IGF-1; 100 ng/ml; recombinant Human LONG R3 IGF-1, Sigma-Aldrich, Cat# I1271, MO, USA), rapamycin (10 ng/ml ...
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bioRxiv - Immunology 2024Quote: ... MASP-3 and MAP-1 was measured in an ELISA assay where 10 µg/ml recombinant human albumin (rHA) (Sigma-Aldrich, A9731) was coated on Nunc MaxiSorp flat-bottom 96-well plates (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2022Quote: ... rabbit polyclonal antibody AB37086 (1:2500) and actin using a rabbit polyclonal antibody from Sigma-Aldrich (#A2066; 1:8000). HRP-tagged secondary antibodies (Bio-Rad ...
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bioRxiv - Cell Biology 2021Quote: ... and incubation overnight at 4°C with primary antibodies (rabbit anti-H3K9me3, Abcam, ab8898, 1:1,000; rabbit anti-H3K27me3, Millipore, 07-449 ...
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bioRxiv - Cell Biology 2021Quote: ... and then incubated overnight at 4°C with primary antibodies (rabbit anti-H3K9me3, Abcam, ab8898, 1:3,000; rabbit anti-H3K27me3, Millipore, 07-449 ...
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bioRxiv - Microbiology 2020Quote: ... horseradish peroxidase-conjugated rabbit anti-rat IgG and horseradish peroxidase-conjugated goat anti-rabbit IgG (Sigma, St. Louis, MO) were used for Western blotting at dilutions of 1:10,000 ...
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bioRxiv - Biochemistry 2021Quote: ... Primary rabbit polyclonal anti-(human Nav1.5), (residues: 1978-2016) (#ASC-013, Alomone Labs) and rabbit polyclonal anti-(actin) (A2066, Sigma), both used at a concentration of 1:200 were followed by a one-hour incubation with secondary Goat anti-(Rabbit IgG-HRP conjugate ...
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bioRxiv - Cell Biology 2023Quote: ... Rabbit or mouse primary antibodies were detected using a horseradish peroxidase-conjugated anti-rabbit IgG (Sigma AP307P, 1:4000) or anti-mouse IgG (Sigma AP308P ...
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bioRxiv - Developmental Biology 2023Quote: Membranes were incubated overnight in primary antibody o/n at 4°C: Rabbit ⍺-Vang (Rawls & Wolff, 2003) diluted 1:1,000 in 5% milk in TBST or Rabbit ⍺-Actin (Sigma) diluted 1:2,000 in 10% Seablock in TBST followed by 3x wash in TBST for 10 minutes each ...
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bioRxiv - Cell Biology 2023Quote: ... Rabbit anti-phospho-ERM (1/5000) (Roubinet et al., 2011) and rabbit anti-actin (Sigma-Aldrich A5060, 1/10,000) were also used ...
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bioRxiv - Cancer Biology 2024Quote: ... samples stained with primary FBXO11 (mouse) and LONP1 (rabbit) antibodies were incubated with Anti-Rabbit PLUS (Sigma-Aldrich DUO92002) and Anti-Mouse MINUS (Sigma-Aldrich DUO92004 ...
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bioRxiv - Molecular Biology 2019Quote: The recombinant IFNε protein was purified using a single-step High-Select High Flow (HF) nickel affinity chromatographic gel (Sigma-Aldrich, Cat. # H0537). The nickel affinity column (1.0 cm × 1.0 cm ...
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bioRxiv - Microbiology 2020Quote: ... were immunized intraperitoneally with 17 μg of recombinant NRP1 b1b2 mixed with an equal volume of complete Freund’s adjuvant (Sigma–Aldrich Chemie, Steinheim, Germany), followed by a booster immunization four weeks later of the same dose mixed with incomplete Freund’s adjuvant (Sigma–Aldrich) ...
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bioRxiv - Biochemistry 2020Quote: ... The production of the (His)6-tagged recombinant proteins was confirmed by Western analysis using mouse monoclonal α-His-horseradish peroxidase conjugated antibodies (Sigma Aldrich, USA). Imaging of the protein bands on the Western blot was conducted using the ECL kit (ThermoScientific ...
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bioRxiv - Neuroscience 2020Quote: 100 μg of recombinant Tau 2N4R were incubated for 2h at room temperature in 100 mM sodium citrate pH 6 (Sigma, cat. no. W302600), 100 mM sodium cyanoborohydride (Sigma ...