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Citations for Millipore Sigma :
2501 - 2550 of 4463 citations for Recombinant Human IL12B Fc tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... AML cells were washed and cultured in Gartner’s medium (Alpha-MEM, 12.5% horse serum, 12.5% FCS, 1μM hydrocortisone (Sigma-Aldrich, St. Louis, MO, USA), 1% pen-strep (Sigma-Aldrich ...
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bioRxiv - Immunology 2023Quote: ... The tissue was incubated in RPMI/5%FCS/10mMHEPES for 5min before being digested in 10ml RPMI/5% FCS/10mM HEPES supplemented with 0.4mg/ml type VIII collagenase (Sigma Aldrich, UK) and 40μg/ml DNase I (Roche ...
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bioRxiv - Immunology 2023Quote: ... samples were serially diluted in MEM containing 2% FCS and 100 Units Penicillin / 0.1 mg Streptomycin (P/S) (Millipore Sigma, Germany). Vero E6 cells were incubated with 100 µl of ten-fold sample dilutions added in quadruplicates for 1 h at 37°C before 100 µl MEM containing 2% FCS and P/S were added per well and plates were incubated for 5 days at 37°C and 5% CO2 ...
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bioRxiv - Immunology 2023Quote: ... THP1 dual reporter cells were maintained in RPMI (Nacalai Tesque Inc.,Kyoto, Japan) supplemented with 10% FCS (Sigma-Aldrich, Missouri, USA), 1% penicillin/streptomycin (Nacalai Tesque Inc.,Kyoto ...
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bioRxiv - Cancer Biology 2023Quote: ... Newly diagnosed leukemic cells was partly used directly after sampling for in vitro and in vivo experiments and partly frozen in Foetal Calf Serum (FCS) containing 10% of Dimethylsufoxide (DMSO) (D8418, Sigma-Aldrich). Human T-ALL samples are described in Supplementary Table 1.
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bioRxiv - Cancer Biology 2023Quote: ... The gradients were then displaced into a UA-6 continuous UV detector (Teledyne ISCO) using a syringe pump (Brandel) containing Fluorinert FC-40 (Sigma #F9755) at a speed of 0.75 ml/min ...
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bioRxiv - Immunology 2023Quote: ... were co-cultured in round-bottomed 96-well plates in DC medium (RPMI-1640, 10% FCS, 100 U/mL Penicillin, 100 μg/mL Streptomycin, 2 mM L-glutamine [Sigma-Aldrich]) the presence of indicated concentrations of OVA323-339 peptide (Sigma ...
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bioRxiv - Microbiology 2023Quote: ... Isolates were standardised to 1×106 cells/mL in Roswell Park Memorial Institute (RPMI) media supplemented with 5% (v/v) foetal calf serum (FCS [Sigma-Aldrich]) to simulate organic load ...
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bioRxiv - Microbiology 2024Quote: ... BEAS-2B cells grown in 6-wells plates were first transduced with lentiviruses encoding Cas9 diluted in infection medium (DMEM, 2% FCS) containing 8 μg/ml Polybrene (Sigma Aldrich). At 16 h post transduction ...
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bioRxiv - Microbiology 2024Quote: ... The cells were maintained with DMEM+10%FCS for 3 hours and fixed with acetone/methanol (1:1 ratio) and stained with Giemsa stain (Sigma-Aldrich). Stained cell images were taken by EVOS XL imaging system (Life Technologies).
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bioRxiv - Cancer Biology 2024Quote: ... Cells (named T-ALL#2) were frozen and conserved in 90% Fetal Calf Serum (FCS) and 10% DMSO solution (D5879, Sigma Aldrich), or injected into CD42.1.2 C57BL/6 recipient mice ...
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bioRxiv - Synthetic Biology 2024Quote: ... we first prepared a 2% (w/w) mixture of surfactant (008 FluoroSurfactant, RAN Biotechnologies) and oil (FC-40 FluorinertTM, Sigma-Aldrich). Using Eppendorf DNA Lobind® tubes ...
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bioRxiv - Cancer Biology 2024Quote: ... Cells were washed with warm PBS + 1%FCS and incubated with 1:50 DAPI (70µg/ml, Sigma-Aldrich, cat. No. D9542) and 1:50 Annexin V-APC (Biolegend ...
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bioRxiv - Cell Biology 2021Quote: ... at a concentration of 1:500 was used to detect myc tagged ATR and incubated at room temperature for 1 h in blocking buffer (1 % BSA [Sigma], 0.2 % Tween-20 in 1x PBS) in a wet chamber ...
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bioRxiv - Biochemistry 2021Quote: ... and purified using DDDDK-tagged protein purification gel (MBL, Japan) followed by elution with 0.1 mg/ml FLAG Peptide (Sigma-Aldrich Co., St. Louis, Missouri, the US). To remove the flag peptide ...
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bioRxiv - Plant Biology 2021Quote: ... We prepared InsP7 by using 100ng of Mouse IP6K1 (mIP6K1) recombinant protein along with 100 µM of InsP6 (Sigma, USA) in a buffer containing 20 mM HEPES (pH 7.5) ...
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bioRxiv - Microbiology 2020Quote: ... T cells were then infected with HIVBaL and cultured for up to 7 days in RPMI medium additionally supplemented with 10 U/ml recombinant interleukin-2 (Sigma). The dividing cell lines HEK293T (ATCC ...
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bioRxiv - Epidemiology 2019Quote: ... and recombinant heat labile toxin β subunit protein [15,52,53] from enterotoxigenic Escherichia coli (ETEC LT β subunit) were purchased from Sigma Chemical (St ...
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bioRxiv - Bioengineering 2021Quote: ... Columns were washed with 20 column volumes PBS and recombinant proteins were eluted with 100 mM β-d-lactose (Sigma) prepared in PBS ...
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bioRxiv - Biochemistry 2021Quote: ... standard conditions (ii) with or without recombinant enzyme Agalsidase alpha (5µl/ml) (Takeda) and (iii) with or without wortmannin (75nM) (Sigma-Aldrich). Of note ...
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bioRxiv - Biochemistry 2020Quote: ... The recombinant proteins were overexpressed in E.coli BL21 (DE3) at 17 °C overnight and then purified by Glutathione-agarose (Sigma #G4510). Briefly ...
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bioRxiv - Neuroscience 2021Quote: ... Sample was then diluted 8-fold with 50 mM HEPES pH 8.0 and digested by the addition of 5 μg TrypZean recombinant trypsin (Sigma-Aldrich) and incubation for 8 hours at 30 °C ...
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bioRxiv - Plant Biology 2021Quote: ... residual trypsin activity was measured by monitoring the change in absorbance at 247 nm in presence of increasing concentration of recombinant purified Kunitz Trypsin inhibitor (KTI) when incubated with p-toluene-sulfonyl-L-Arg methyl ester (Sigma). To study the inhibitory action of P235 on KTI action ...
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bioRxiv - Plant Biology 2021Quote: ... Substrate specificity of recombinant AtPME2 activity was determined at pH 7.5 and 28°C using commercial citrus pectin (Sigma-Aldrich, DM >85% ...
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bioRxiv - Systems Biology 2020Quote: ... At time 0 medium was removed from cells and recombinant mouse tumor necrosis factor alpha (TNF-α, Sigma-Aldrich, #T7539) was added at concentrations 0-100 ng/mL as 5-minute pulse ...
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bioRxiv - Microbiology 2021Quote: Six-week-old Balb/cN mice were primed subcutaneously either with 30 μg of recombinant SARS-Cov-2 Spike protein (S) or Spike-RBD (RBD) in the complete Freund’s adjuvant (SIGMA-ALDRICH) and boosted three times at three-week intervals with 20 μg of the same antigen in the incomplete Freund`s adjuvant ...
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bioRxiv - Plant Biology 2021Quote: ... substrate specificity assays for recombinant PcIBMS1 and PcIPMS1 were determined with a spectrophotometric end-point assay with DTNB (Sigma-Aldrich). An aliquot of 1-5 μL of the protein preparation was added to an enzyme assay mixture (100 mM Tris ...
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bioRxiv - Biochemistry 2022Quote: ... and fractions judged by SDS-PAGE to contain the recombinant protein were pooled and desalted by size exclusion chromatography using 30K centrifugal spin column (Millipore).
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bioRxiv - Biochemistry 2022Quote: ... recombinant proteins were purified by anti-c-Myc Antibody Beads (10D11; Wako) or Anti-FLAG M2 affinity gel (Sigma-Aldrich) following the standard protocol ...
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bioRxiv - Molecular Biology 2021Quote: ... The fractions containing the recombinant protein were pooled and concentrated through 15 mL 10-kDa MWCO Amicon-Ultra centrifugal filters (Millipore). Protein concentrations were measured by absorbance at 280 nm in nanodrop (Thermo Scientific) ...
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bioRxiv - Microbiology 2021Quote: ... pFPV-TurboFP650 recombinant plasmids were selected on Trypticase Soya Agar (TSA - BioMérieux) containing 100 µg/mL of carbenicillin (Sigma-Aldrich) and clones which showed a purple color were selected for restriction analysis ...
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bioRxiv - Microbiology 2021Quote: ... The rabbit polyclonal anti‐Per1 antibody was generated against recombinant Per1 protein (recPer1) corresponding to bases 55‐462 of per1 CDS (Aste010406) expressed in pET‐42a (Novagen) commercially (GL Biochem Ltd ...
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bioRxiv - Immunology 2022Quote: ... 20 ng/ml recombinant IL-1β (Preprotech) or 100 ng/ml lipopolysaccharide from Escherichia coli O55:B5 (LPS, Sigma-Aldrich) for 6h at 37°C ...
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bioRxiv - Developmental Biology 2019Quote: ... The serum-containing mESCs medium was composed of high glucose DMEM (HyClone) supplemented with 1000 U/mL recombinant mouse leukaemia inhibitory factor (LIF) (Millipore), 100 μM non-essential amino acids (NEAA ...
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bioRxiv - Plant Biology 2019Quote: ... using a VP-ITC microcalorimeter (MicroCal) as described [34] after extensive dialysis of the purified recombinant protein in Chelex 100 (Sigma) to remove contaminating divalent cations ...
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bioRxiv - Developmental Biology 2020Quote: ... The trophectoderm was removed by immunosurgery and “epiblasts” were grown in gelatinised dishes in the presence of NDiff + 2i and ESGRO (recombinant mouse LIF Protein, Millipore) to establish ESC lines.
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bioRxiv - Cancer Biology 2021Quote: Primary HPMECs in EBM2 medium or ST1.6R cells in DMEM/1% FBS were cultured overnight and stimulated with 2 μg/ml of recombinant TNC (Merck Millipore) for 24 h ...
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bioRxiv - Cell Biology 2019Quote: ... Immune sera were generated by immunization of 6 weeks-old female NMRI mice (Charles Liver Laboratories) via subcutaneous injection of 100 µg recombinant protein emulsified in Freund’s incomplete adjuvant (Sigma-Aldrich) followed by a boost after 4 weeks with 50 µg of recombinant protein ...
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bioRxiv - Biochemistry 2019Quote: ... Proteins were digested for at least 14 h (overnight) with an animal-free recombinant bovine trypsin expressed in corn (Trypzean T3568 from Sigma) at 1/50 w/w and RT ...
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bioRxiv - Microbiology 2020Quote: ... chondrophila were first evidenced by incubating the purified recombinant enzymes overnight at 30°C with 10 mg.mL-1 glycogen from rabbit liver (Sigma-Aldrich) and 20 mM inorganic phosphate in 20 mM Tris-HCl buffer (pH 6.8) ...
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bioRxiv - Microbiology 2021Quote: ... kinetics was recorded as the HA loaded sensors were dipped into 1.5-fold serially diluted concentrations of recombinant GM2 (Sigma Aldrich). The reaction was carried out in a 1x kinetics buffer comprising of 1x PBS ...
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bioRxiv - Physiology 2020Quote: ... tau/NTF or N-terminal (243-441) tau/CTF cDNA was used to produce recombinant tau protein in Escherichia coli BL21 (DE3) (Merck Millipore). Purification was performed as described previously (Hasegawa et al. ...
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bioRxiv - Biophysics 2021Quote: Catalytic activity was evaluated in vitro using 20 pmol of purified recombinant proteins in a 200 µl reaction buffer supplemented with 20 mM p-nitrophenyl phosphate (Sigma) as substrate ...
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bioRxiv - Microbiology 2021Quote: ... Purification of the recombinant mucoricin was performed by Ni-NTA matrix affinity purification according to the manufacturers’ instructions (Sigma-Aldrich). The purity of the protein was confirmed by SDS-PAGE and quantified by a modified Lowry protein assay (Pierce).
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bioRxiv - Molecular Biology 2022Quote: ... and 1 μg of FhNEJ-Teg or recombinant protein in the presence or absence of 15 ng t-PA (Sigma) or 10 ng u-PA (Sigma) ...
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bioRxiv - Neuroscience 2023Quote: ... we randomly assigned Ai9:Tmem119cre mice to receive an intravenous injection of control solution or 0.05 mg/kg of recombinant IFNγ from mouse (Sigma #I4777), minutes before initiating the rupture of cortical capillaries and then 12h later ...
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bioRxiv - Molecular Biology 2024Quote: ... Further confirmation of purification of the recombinant protein was obtained by immunoblots probed with mouse anti-polyhistidine IgG (Sigma-Aldrich) and goat anti-mouse alkaline phosphatase-conjugated antibodies (Thermo Fisher Scientific) ...
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bioRxiv - Immunology 2024Quote: BMDM were polarized or not with recombinant IL-4 (10 ng mL−1, peprotech) for 24 hours and with LPS (10 ng/ml, Sigma) for 8 hours ...
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bioRxiv - Molecular Biology 2022Quote: ... Jurkat cells were infected with the recombinant viruses in the presence of 8 µg/ml polybrene (hexadimethrine bromide, Sigma-Aldrich) for 15 hours at 37°C ...
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bioRxiv - Microbiology 2023Quote: ... were subcutaneously injected with 100 µg of the pure recombinant protein emulsified in Freund’s incomplete adjuvant (Sigma Aldrich, Taufkirchen, DE) followed by a boost after 4 weeks ...